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The map label for this gene is gyrB [H]

Identifier: 21228521

GI number: 21228521

Start: 2881210

End: 2883114

Strand: Direct

Name: gyrB [H]

Synonym: MM_2419

Alternate gene names: 21228521

Gene position: 2881210-2883114 (Clockwise)

Preceding gene: 21228520

Following gene: 21228522

Centisome position: 70.34

GC content: 47.03

Gene sequence:

>1905_bases
ATGAGTGATAAACAGGTTTATGACGCTTCGCACATCCAGGTTCTTGAAGGCCTGGAGGCTGTCCGAAAACGCCCGAGCAT
GTATATAGGGAGCACAGATGGGCGCGGGCTCCACCACCTAGTCTATGAGGTTGTAGACAACAGTATTGATGAAGCTCTTG
CAGGCTTCTGTTCAGAGATCGATGTCACGATCAATCCCAATGGAAGCGTGACTGTAATTGACAATGGAAGGGGTATCCCT
ATCGATCCGCACCCGGTGCATAAGAAATCGGCACTTGAGGTCGTGATGACGATTTTGCACGCGGGAGGAAAATTTGACAA
GAATACTTACAAGGTCTCCGGGGGCCTGCACGGGGTGGGGGTCTCGGTAGTAAATGCCCTTTCGGAATGGACCGAAGTGG
AAGTAAGCAGAGATGGAAAGAAGTACTTCCAGCGTTATATCCGCGGAAAACCTGAATCCGATGTCCGGGAAATAGGAACT
TCGGATACCACAGGCACAAAAACCACTTTCAAACCGGACACAAAAATCTTTGAGACAACTGACTTCCAGTATGATATCCT
GTTAAATAGGCTCAGGGAACTTGCTTTTCTGAACCGCGGCCTTATAATCAATCTGAAGGATTTAAGAAGCCCCGAAGGTC
AGGCAGAGACTTTCAACTATAGCGGAGGGATAATTGAGTTTGTGGAATATCTTAATAAGAAAAAACAGCCCCTCCATGAA
AAACCGATCTATTTCGAAAGACAGAGAGATGATATGGTAGTGGAAATTGCAATGCAGTACACAAACGGTTACACTGAAAA
CGTGTACTCTTTTGCAAACAACATTAACACCCACGAAGGCGGGACCCATATTATCGGTTTTAAGACTGCCCTTACAAGGG
TAGCCAACGATTACATAAAGGCCAATAAGCTCTCCAAGGAAGACGAAAAGCTGACAGGAGACGATGTAAGGGAAGGTCTG
ACTGCGATTATCAGTGTCAAGCTAATGGAGCCCCAGTTTGAAGGGCAGACCAAGACAAAGCTCGGAAACAGTGAGGTCAA
AGGGATAGTTGACTCCCTTGTAACTGACGGGCTTGCAGAGTACTTTGAAGAAAACCCCAAAGTTGCCAATATTATCCTTG
ACAAAGCCCTTCTTGCCCAGAGAGCTAGAGAGGCTGCAAAGAAGGCAAGGGAACTTACGCGGAGAAAGAGTGCGCTTGAG
GTCAGCACCCTGCCGGGAAAACTTGCAGACTGTTCTAATAAGAACCCTGCAGAATGTGAGATCTATATTGTTGAAGGAGA
CTCTGCAGGCGGTTCTGCAAAGCAGGGCAGAGACCGAAGCTTCCAGGCAATTCTGCCTCTCAGGGGCAAGATCCTTAATG
TAGAAAAGTCCAGGCTTGCAAAGATCCTGAAAAACAACGAGGTCATTTCACTTATTACAGCCATAGGGACAGGAGTAAGT
GAAGACTTTGATCTTGAAAACGCACGCTACCACAAAATCATTATTATGACCGATGCCGATGTGGACGGCGCTCATATCCG
AACCCTTCTTCTGACCCTGTTCTTCCGTTATATGAGGCATCTGATAGATGCAGGGTACGTATACATTGCCCAGCCGCCTC
TTTACAAGATAAAGAAAGGAAAAATGGATTACTATGCGTATTCGGATAAAGAGCTGGCAGATAAGTTAAAAGAACTCGGG
GAGAAAGGCGTTGCCATCCAGCGTTACAAAGGTCTTGGAGAAATGAACCCTGGGCAGCTCTGGGAAACCACCATGGACCC
TGAGAGCCGGACCCTTTTGCAGGTTACTCTGGAAGATGCAATCCGAGCTGACGAAATCTTCCGTGTACTTATGGGAGATG
AGGTCGAACCTCGCAGAAACTTCATCGAAACACATGCAAAAGAGGTTGTGGACCTGGATATCTGA

Upstream 100 bases:

>100_bases
ATTTCGAACCGAGTTTCCCTGCGAGTTTTAAAAAATGTATAATATGTTACGATATTTAAAATAAATAACTTTACCAGATT
TTTATGGAGAATAAACCACG

Downstream 100 bases:

>100_bases
GGTGGTGAAAAATGGCAGAAAATGAAGACAAAAACAAATCAGAAGCAGAAATGAAGAAATCATACCAGGCAACTCTTATT
CCTGAAAGTCCAGTAGAAAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSDKQVYDASHIQVLEGLEAVRKRPSMYIGSTDGRGLHHLVYEVVDNSIDEALAGFCSEIDVTINPNGSVTVIDNGRGIP
IDPHPVHKKSALEVVMTILHAGGKFDKNTYKVSGGLHGVGVSVVNALSEWTEVEVSRDGKKYFQRYIRGKPESDVREIGT
SDTTGTKTTFKPDTKIFETTDFQYDILLNRLRELAFLNRGLIINLKDLRSPEGQAETFNYSGGIIEFVEYLNKKKQPLHE
KPIYFERQRDDMVVEIAMQYTNGYTENVYSFANNINTHEGGTHIIGFKTALTRVANDYIKANKLSKEDEKLTGDDVREGL
TAIISVKLMEPQFEGQTKTKLGNSEVKGIVDSLVTDGLAEYFEENPKVANIILDKALLAQRAREAAKKARELTRRKSALE
VSTLPGKLADCSNKNPAECEIYIVEGDSAGGSAKQGRDRSFQAILPLRGKILNVEKSRLAKILKNNEVISLITAIGTGVS
EDFDLENARYHKIIIMTDADVDGAHIRTLLLTLFFRYMRHLIDAGYVYIAQPPLYKIKKGKMDYYAYSDKELADKLKELG
EKGVAIQRYKGLGEMNPGQLWETTMDPESRTLLQVTLEDAIRADEIFRVLMGDEVEPRRNFIETHAKEVVDLDI

Sequences:

>Translated_634_residues
MSDKQVYDASHIQVLEGLEAVRKRPSMYIGSTDGRGLHHLVYEVVDNSIDEALAGFCSEIDVTINPNGSVTVIDNGRGIP
IDPHPVHKKSALEVVMTILHAGGKFDKNTYKVSGGLHGVGVSVVNALSEWTEVEVSRDGKKYFQRYIRGKPESDVREIGT
SDTTGTKTTFKPDTKIFETTDFQYDILLNRLRELAFLNRGLIINLKDLRSPEGQAETFNYSGGIIEFVEYLNKKKQPLHE
KPIYFERQRDDMVVEIAMQYTNGYTENVYSFANNINTHEGGTHIIGFKTALTRVANDYIKANKLSKEDEKLTGDDVREGL
TAIISVKLMEPQFEGQTKTKLGNSEVKGIVDSLVTDGLAEYFEENPKVANIILDKALLAQRAREAAKKARELTRRKSALE
VSTLPGKLADCSNKNPAECEIYIVEGDSAGGSAKQGRDRSFQAILPLRGKILNVEKSRLAKILKNNEVISLITAIGTGVS
EDFDLENARYHKIIIMTDADVDGAHIRTLLLTLFFRYMRHLIDAGYVYIAQPPLYKIKKGKMDYYAYSDKELADKLKELG
EKGVAIQRYKGLGEMNPGQLWETTMDPESRTLLQVTLEDAIRADEIFRVLMGDEVEPRRNFIETHAKEVVDLDI
>Mature_633_residues
SDKQVYDASHIQVLEGLEAVRKRPSMYIGSTDGRGLHHLVYEVVDNSIDEALAGFCSEIDVTINPNGSVTVIDNGRGIPI
DPHPVHKKSALEVVMTILHAGGKFDKNTYKVSGGLHGVGVSVVNALSEWTEVEVSRDGKKYFQRYIRGKPESDVREIGTS
DTTGTKTTFKPDTKIFETTDFQYDILLNRLRELAFLNRGLIINLKDLRSPEGQAETFNYSGGIIEFVEYLNKKKQPLHEK
PIYFERQRDDMVVEIAMQYTNGYTENVYSFANNINTHEGGTHIIGFKTALTRVANDYIKANKLSKEDEKLTGDDVREGLT
AIISVKLMEPQFEGQTKTKLGNSEVKGIVDSLVTDGLAEYFEENPKVANIILDKALLAQRAREAAKKARELTRRKSALEV
STLPGKLADCSNKNPAECEIYIVEGDSAGGSAKQGRDRSFQAILPLRGKILNVEKSRLAKILKNNEVISLITAIGTGVSE
DFDLENARYHKIIIMTDADVDGAHIRTLLLTLFFRYMRHLIDAGYVYIAQPPLYKIKKGKMDYYAYSDKELADKLKELGE
KGVAIQRYKGLGEMNPGQLWETTMDPESRTLLQVTLEDAIRADEIFRVLMGDEVEPRRNFIETHAKEVVDLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=635, Percent_Identity=25.511811023622, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI19913406, Length=604, Percent_Identity=25.1655629139073, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI48994957, Length=550, Percent_Identity=56.5454545454545, Blast_Score=600, Evalue=1e-172,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=40.4458598726115, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17535065, Length=603, Percent_Identity=26.865671641791, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=24.3333333333333, Blast_Score=127, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=205, Percent_Identity=29.2682926829268, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=639, Percent_Identity=26.4475743348983, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=663, Percent_Identity=25.4901960784314, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71069; Mature: 70938

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKQVYDASHIQVLEGLEAVRKRPSMYIGSTDGRGLHHLVYEVVDNSIDEALAGFCSEI
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCC
DVTINPNGSVTVIDNGRGIPIDPHPVHKKSALEVVMTILHAGGKFDKNTYKVSGGLHGVG
EEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHH
VSVVNALSEWTEVEVSRDGKKYFQRYIRGKPESDVREIGTSDTTGTKTTFKPDTKIFETT
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEECCCCCEEECC
DFQYDILLNRLRELAFLNRGLIINLKDLRSPEGQAETFNYSGGIIEFVEYLNKKKQPLHE
CCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCC
KPIYFERQRDDMVVEIAMQYTNGYTENVYSFANNINTHEGGTHIIGFKTALTRVANDYIK
CCCEEECCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHH
ANKLSKEDEKLTGDDVREGLTAIISVKLMEPQFEGQTKTKLGNSEVKGIVDSLVTDGLAE
HHCCCCHHHCCCCHHHHHHHHHHHEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
YFEENPKVANIILDKALLAQRAREAAKKARELTRRKSALEVSTLPGKLADCSNKNPAECE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHCCCCCCCCEEE
IYIVEGDSAGGSAKQGRDRSFQAILPLRGKILNVEKSRLAKILKNNEVISLITAIGTGVS
EEEEECCCCCCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHCCCCHHHHHHHHCCCCC
EDFDLENARYHKIIIMTDADVDGAHIRTLLLTLFFRYMRHLIDAGYVYIAQPPLYKIKKG
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCC
KMDYYAYSDKELADKLKELGEKGVAIQRYKGLGEMNPGQLWETTMDPESRTLLQVTLEDA
CCCEEECCCHHHHHHHHHHHHCCCEEHHHCCCCCCCCCCCEECCCCCCCCEEEEEEHHHH
IRADEIFRVLMGDEVEPRRNFIETHAKEVVDLDI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDKQVYDASHIQVLEGLEAVRKRPSMYIGSTDGRGLHHLVYEVVDNSIDEALAGFCSEI
CCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCC
DVTINPNGSVTVIDNGRGIPIDPHPVHKKSALEVVMTILHAGGKFDKNTYKVSGGLHGVG
EEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHH
VSVVNALSEWTEVEVSRDGKKYFQRYIRGKPESDVREIGTSDTTGTKTTFKPDTKIFETT
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCEEECCCCCEEECC
DFQYDILLNRLRELAFLNRGLIINLKDLRSPEGQAETFNYSGGIIEFVEYLNKKKQPLHE
CCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCC
KPIYFERQRDDMVVEIAMQYTNGYTENVYSFANNINTHEGGTHIIGFKTALTRVANDYIK
CCCEEECCCCCEEEEEEHHHCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHH
ANKLSKEDEKLTGDDVREGLTAIISVKLMEPQFEGQTKTKLGNSEVKGIVDSLVTDGLAE
HHCCCCHHHCCCCHHHHHHHHHHHEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHH
YFEENPKVANIILDKALLAQRAREAAKKARELTRRKSALEVSTLPGKLADCSNKNPAECE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHCCCCCCCCEEE
IYIVEGDSAGGSAKQGRDRSFQAILPLRGKILNVEKSRLAKILKNNEVISLITAIGTGVS
EEEEECCCCCCCCCCCCCCCCEEEECCCCEEECCCHHHHHHHHCCCCHHHHHHHHCCCCC
EDFDLENARYHKIIIMTDADVDGAHIRTLLLTLFFRYMRHLIDAGYVYIAQPPLYKIKKG
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCC
KMDYYAYSDKELADKLKELGEKGVAIQRYKGLGEMNPGQLWETTMDPESRTLLQVTLEDA
CCCEEECCCHHHHHHHHHHHHCCCEEHHHCCCCCCCCCCCEECCCCCCCCEEEEEEHHHH
IRADEIFRVLMGDEVEPRRNFIETHAKEVVDLDI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]