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The map label for this gene is eche [H]

Identifier: 21228426

GI number: 21228426

Start: 2782040

End: 2783116

Strand: Direct

Name: eche [H]

Synonym: MM_2324

Alternate gene names: 21228426

Gene position: 2782040-2783116 (Clockwise)

Preceding gene: 21228425

Following gene: 21228427

Centisome position: 67.92

GC content: 41.5

Gene sequence:

>1077_bases
ATGACAACTGTAATACCTTTTGGTCCTCAGCACCCCGTTTTACCCGAACCGGTAGCGTTGAAACTTGAGCTTGACGACGA
TATTGTTGTTGGAGCACTTCCTTCAATAGGCTATGTGCACAGAGGGCTTGAGAAGTTCATAGACACAAAGGACTATGTTC
AAACAACATACATTTGTGAAAGAATCTGTGGAATATGCAGTGCCCTCCATGGTATAACTTATACACGGGCAGTTGAGAAA
TTATTCGATTTAGAACCCCCTGAAAGAGCTCAGTATATCAGGGTAATAGTTGGTGAACTCAATAGACTCCACAGTCACCT
TCTCTGGCTTGGTCTGTTTGCTGATGGTTTTGGATTTGAAAGCCTTTTTTATGAATGCTGGAAGTACAGAGAAGGAGTAC
AGGATGCAGCGGAAAGAATCTGTGGAAACAGGGTTATACACTCAATATCAAAGGTCGGAGGAGTTACCAGAGATTTGACC
AAAGAACATACTGATATGCTTCTGAAACTGTGCGATACACTGGAAGACCAAGTTAAAAACATTGAAAAGGTGTTCGTAAA
TAATTACACCGTTAAGAAAAGAACTAAGGGATTAGGTGTAATGTCAAAGCAGGTAGCATATGAGTTGGGAACTGCCGGTC
CTACATTGAGAGGAAGCGATAACGCCATTGACGTAAGAGATATAGATGACTGGGCAATTTATAAGGATCTCGGTTTTAAG
ACAGCTGTGGAGAAAGACGGAGATTGTTACTCAAGAACTAAAGTAAGGATCAACGAAGTACTTAATTCTATTGAAATAAT
AAGAAATGCAATCTCCAGGCTGCCTAATGGCGACATTTCAGTACCTGTAAAGGGCTTCCCTACCGGTGAAGCAATTATGA
GAACAGAGCAGCCCAGAGGTGAAGTTGTATATTACGTGAAAGGTAACGGTACTAAAAACCTGGAAAGGCTTAAAATTAGG
ACTCCTACCTTTGCAAACATACCTTCACTGCTAGTAATGCTCCCAGGCGTAAAACTCGCTGATGTTCCTATAATTGTACT
CACTATAGACCCCTGCATTAGCTGCACTGAAAGATGA

Upstream 100 bases:

>100_bases
ACTTTCGAAGGTTTAGCAATAGACTATAAAGGCTACCTTTACTTAACGCCAAACAGTCCGAAAGCTCCTTTAGCCTAAAG
CAATTTGAGAGGAGAATCAT

Downstream 100 bases:

>100_bases
AGTGGAAGGGTGATTAAAATGGGTATGCTAAACCTTGTATTATCAAACATTTCTCATAAACCGGCTACCAGACTTTATCC
CTTCGAGATAAGGGAAACTT

Product: ech hydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTTVIPFGPQHPVLPEPVALKLELDDDIVVGALPSIGYVHRGLEKFIDTKDYVQTTYICERICGICSALHGITYTRAVEK
LFDLEPPERAQYIRVIVGELNRLHSHLLWLGLFADGFGFESLFYECWKYREGVQDAAERICGNRVIHSISKVGGVTRDLT
KEHTDMLLKLCDTLEDQVKNIEKVFVNNYTVKKRTKGLGVMSKQVAYELGTAGPTLRGSDNAIDVRDIDDWAIYKDLGFK
TAVEKDGDCYSRTKVRINEVLNSIEIIRNAISRLPNGDISVPVKGFPTGEAIMRTEQPRGEVVYYVKGNGTKNLERLKIR
TPTFANIPSLLVMLPGVKLADVPIIVLTIDPCISCTER

Sequences:

>Translated_358_residues
MTTVIPFGPQHPVLPEPVALKLELDDDIVVGALPSIGYVHRGLEKFIDTKDYVQTTYICERICGICSALHGITYTRAVEK
LFDLEPPERAQYIRVIVGELNRLHSHLLWLGLFADGFGFESLFYECWKYREGVQDAAERICGNRVIHSISKVGGVTRDLT
KEHTDMLLKLCDTLEDQVKNIEKVFVNNYTVKKRTKGLGVMSKQVAYELGTAGPTLRGSDNAIDVRDIDDWAIYKDLGFK
TAVEKDGDCYSRTKVRINEVLNSIEIIRNAISRLPNGDISVPVKGFPTGEAIMRTEQPRGEVVYYVKGNGTKNLERLKIR
TPTFANIPSLLVMLPGVKLADVPIIVLTIDPCISCTER
>Mature_357_residues
TTVIPFGPQHPVLPEPVALKLELDDDIVVGALPSIGYVHRGLEKFIDTKDYVQTTYICERICGICSALHGITYTRAVEKL
FDLEPPERAQYIRVIVGELNRLHSHLLWLGLFADGFGFESLFYECWKYREGVQDAAERICGNRVIHSISKVGGVTRDLTK
EHTDMLLKLCDTLEDQVKNIEKVFVNNYTVKKRTKGLGVMSKQVAYELGTAGPTLRGSDNAIDVRDIDDWAIYKDLGFKT
AVEKDGDCYSRTKVRINEVLNSIEIIRNAISRLPNGDISVPVKGFPTGEAIMRTEQPRGEVVYYVKGNGTKNLERLKIRT
PTFANIPSLLVMLPGVKLADVPIIVLTIDPCISCTER

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=372, Percent_Identity=31.4516129032258, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI260898743, Length=370, Percent_Identity=31.3513513513514, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1789076, Length=361, Percent_Identity=35.1800554016621, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788832, Length=359, Percent_Identity=33.983286908078, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI145693162, Length=381, Percent_Identity=26.509186351706, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=383, Percent_Identity=28.1984334203655, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17555284, Length=383, Percent_Identity=28.1984334203655, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI221459469, Length=377, Percent_Identity=29.973474801061, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24638644, Length=372, Percent_Identity=29.3010752688172, Blast_Score=174, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 40085; Mature: 39954

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVIPFGPQHPVLPEPVALKLELDDDIVVGALPSIGYVHRGLEKFIDTKDYVQTTYICE
CCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHH
RICGICSALHGITYTRAVEKLFDLEPPERAQYIRVIVGELNRLHSHLLWLGLFADGFGFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SLFYECWKYREGVQDAAERICGNRVIHSISKVGGVTRDLTKEHTDMLLKLCDTLEDQVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEKVFVNNYTVKKRTKGLGVMSKQVAYELGTAGPTLRGSDNAIDVRDIDDWAIYKDLGFK
HHHHHHCCEEEHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHCCCH
TAVEKDGDCYSRTKVRINEVLNSIEIIRNAISRLPNGDISVPVKGFPTGEAIMRTEQPRG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCC
EVVYYVKGNGTKNLERLKIRTPTFANIPSLLVMLPGVKLADVPIIVLTIDPCISCTER
CEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCEECCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TTVIPFGPQHPVLPEPVALKLELDDDIVVGALPSIGYVHRGLEKFIDTKDYVQTTYICE
CCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHH
RICGICSALHGITYTRAVEKLFDLEPPERAQYIRVIVGELNRLHSHLLWLGLFADGFGFE
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SLFYECWKYREGVQDAAERICGNRVIHSISKVGGVTRDLTKEHTDMLLKLCDTLEDQVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IEKVFVNNYTVKKRTKGLGVMSKQVAYELGTAGPTLRGSDNAIDVRDIDDWAIYKDLGFK
HHHHHHCCEEEHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHCCCH
TAVEKDGDCYSRTKVRINEVLNSIEIIRNAISRLPNGDISVPVKGFPTGEAIMRTEQPRG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCC
EVVYYVKGNGTKNLERLKIRTPTFANIPSLLVMLPGVKLADVPIIVLTIDPCISCTER
CEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCEECCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]