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The map label for this gene is echF [H]

Identifier: 21228427

GI number: 21228427

Start: 2783126

End: 2783506

Strand: Direct

Name: echF [H]

Synonym: MM_2325

Alternate gene names: 21228427

Gene position: 2783126-2783506 (Clockwise)

Preceding gene: 21228426

Following gene: 21228428

Centisome position: 67.94

GC content: 40.94

Gene sequence:

>381_bases
GTGATTAAAATGGGTATGCTAAACCTTGTATTATCAAACATTTCTCATAAACCGGCTACCAGACTTTATCCCTTCGAGAT
AAGGGAAACTTATAAAGAGTTTAAAGGAAGGATTGTTATCAATCCTGAAAACTGTATCCTCTGCGGGCTATGTCAAAAGA
AATGTCCACCGGATGCGATTACAGTTACCAAAGCAGACAAAACGTGGGAAATCAACCTGTTCCGGTGTATTATGTGCACA
GAGTGCGTTACTGGTTGCCCGAAAGACTGCCTATCAATATCCAATGAAAGGCCAAAAACCGGTGCGAAAGAAACTGTTAA
AGTAGTGGTTCCGATAGTAGAGAAACCAAAAGCGGCATCAAAAGCAGCGTCTCCAAAATAA

Upstream 100 bases:

>100_bases
ACTGCTAGTAATGCTCCCAGGCGTAAAACTCGCTGATGTTCCTATAATTGTACTCACTATAGACCCCTGCATTAGCTGCA
CTGAAAGATGAAGTGGAAGG

Downstream 100 bases:

>100_bases
AAATTTCATTTAAATTTTTATTTTTCTACTGGAATCAACGTTATTTTTTCAGTTTTCCGGTTAAAAACAATTTTAAGTTC
CCAAGTCTAAAAAATAGTTT

Product: ech hydrogenase subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MIKMGMLNLVLSNISHKPATRLYPFEIRETYKEFKGRIVINPENCILCGLCQKKCPPDAITVTKADKTWEINLFRCIMCT
ECVTGCPKDCLSISNERPKTGAKETVKVVVPIVEKPKAASKAASPK

Sequences:

>Translated_126_residues
MIKMGMLNLVLSNISHKPATRLYPFEIRETYKEFKGRIVINPENCILCGLCQKKCPPDAITVTKADKTWEINLFRCIMCT
ECVTGCPKDCLSISNERPKTGAKETVKVVVPIVEKPKAASKAASPK
>Mature_126_residues
MIKMGMLNLVLSNISHKPATRLYPFEIRETYKEFKGRIVINPENCILCGLCQKKCPPDAITVTKADKTWEINLFRCIMCT
ECVTGCPKDCLSISNERPKTGAKETVKVVVPIVEKPKAASKAASPK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=95, Percent_Identity=34.7368421052632, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 14014; Mature: 14014

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
10.3 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMGMLNLVLSNISHKPATRLYPFEIRETYKEFKGRIVINPENCILCGLCQKKCPPDAI
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCE
TVTKADKTWEINLFRCIMCTECVTGCPKDCLSISNERPKTGAKETVKVVVPIVEKPKAAS
EEEECCCEEEEHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC
KAASPK
CCCCCC
>Mature Secondary Structure
MIKMGMLNLVLSNISHKPATRLYPFEIRETYKEFKGRIVINPENCILCGLCQKKCPPDAI
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCE
TVTKADKTWEINLFRCIMCTECVTGCPKDCLSISNERPKTGAKETVKVVVPIVEKPKAAS
EEEECCCEEEEHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC
KAASPK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA