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The map label for this gene is rpoB2 [H]

Identifier: 21228375

GI number: 21228375

Start: 2700746

End: 2702341

Strand: Reverse

Name: rpoB2 [H]

Synonym: MM_2273

Alternate gene names: 21228375

Gene position: 2702341-2700746 (Counterclockwise)

Preceding gene: 21228376

Following gene: 21228374

Centisome position: 65.97

GC content: 46.8

Gene sequence:

>1596_bases
GTGCAGCACCATATAGACTCATTTAATAAGTTTATAGACTATGGGCTGCAAAAGATCATTGATGAACAGAGAATAATAGA
AACCGACATTGAGGACACTTATCTCAAGCTCGGCAAGATCCGGGTAGAGCATCCGGTGGTGAAGGAGGCAGACGGAGCGA
TTGAAAAGCTTTACCCGAATGAAGGGAGGCTCAGAAACCTTTCGTATTCAGGCCCCCTCTACCTTGAAATGAGTGTCGTA
AAAGACGGGATAGAGTCAGAGGCAATGGAAGCAAAAATCGGGCAGCTTCCTGTTATGGTCAAGTCCAAAATCTGTAACCT
CCTCGGACTCAGTGAGGTAGAGAAAGTCCGCTATGGAGAAGACCCTCTTGACCCCGGGGGATATTTCATCATCGGTGGTA
CGGAAAGGGTAGTTATGACCCTTGAGGACCTTGCTCCCAACAAAATCCTTGTTGAATTCGGAGAGCGCTACGGTGATGAA
ATAGAGGTTGCAAAAGTATTCTCACAGAAGAGAGGATACAGGGCACTCGTAATTGTGGAGAGAGGCAGGAAGTCCCTGCT
TGAGGTTTCTTTCCCATCGATATCCGGCAGAGTGTACTTTATTACACTTATGAGGGCTCTCGGGATAGTAACTGACGAAG
ATATCGTAAATGCGGTTTCGAGCGATCCCGAAATTATCAAGTTCATGCTGGAAAACCTTGAAGAAGCCGAAGTCGAAACC
CAGGAAGAGGCAATCGAGAAGATCGGGGCAAGGGTTGCTGCAGGGCAGGCCAAGGAATACCAGATAAAAAGGGCAAACTA
CGTAATTGACAGGTATCTTTTCCCACACCTTGGAAACGACATCAGGGACAGGGTCTCAAAAGCTCATTTCCTCGCAAGAA
TGGCAGAGTCCTGTTTTGAGCTGGCACTTAAAAAGCGTACTGAAGACGACAAAGACCACTACGCAAACAAGAGGCTTAAA
CTCGCAGGCGACCTGATGGAAGACCTTTTCAGAGTATCCTTTAACAGGCTGGCAAGGGACATAAAGTACCAGCTCGAACG
CGCAAACATGAGGAACAGGGAACTTAATGTTGCCACAATTGTAAGGGCAGACGTTTTAACTGACCGCCTGCAACACCCGC
TGGCAACAGGAAACTGGGTAGGAGGCAGGACAGGTGTTTCCCAGCTCCTTGACAGGAACGATTACATTTCTACACTTTCT
CACCTTCGCCGCGTAATTTCACCACTTTCCCGTGCCCAGCCTCACTTTGAAGCCCGTGACCTGCACGCAACTCAGTGGGG
GCGTCTCTGTCCTTCCGAAACTCCAGAAGGTCCGAATTGTGGCCTTGTGAAGAACTTTGCAGAAATGGTCGAACTCTCAA
CCGGGACAGAAGAAATAGAGAACATGAAAAAAATACTCTACGACCTTAATGTCGAAAAAGTGGTTGTGAAAGTGCCAGAA
ATCCCGGCAAAAATAAAAGAAATATTACTCGATGAATTCGGGGAAGAGATTGTTGAAGAGTTTGAAGAAACTCCTGAACC
CGAAACCAAATTCAAAGATGATGATTTCTACGATTATTCAGATATGGACGACTCTGGATCTATGTATGGTGACTGA

Upstream 100 bases:

>100_bases
CACCATTTATTGATTTTCATTTTACCAAAGCAAAGAGGGTGCTATCATCGCGTTAGAAAGCAGTATACTGTCGCAGGCCT
ATTTTACACGGGACAAGATA

Downstream 100 bases:

>100_bases
GGGGTTGATGTCAGATGGCTAAAGCAAAAGTATTCATAAACGGAGAGCTCGTCGGGACTCATGACAACCCTGCGGAACTC
GTAGAAGAGCTCAGAAAAAT

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MQHHIDSFNKFIDYGLQKIIDEQRIIETDIEDTYLKLGKIRVEHPVVKEADGAIEKLYPNEGRLRNLSYSGPLYLEMSVV
KDGIESEAMEAKIGQLPVMVKSKICNLLGLSEVEKVRYGEDPLDPGGYFIIGGTERVVMTLEDLAPNKILVEFGERYGDE
IEVAKVFSQKRGYRALVIVERGRKSLLEVSFPSISGRVYFITLMRALGIVTDEDIVNAVSSDPEIIKFMLENLEEAEVET
QEEAIEKIGARVAAGQAKEYQIKRANYVIDRYLFPHLGNDIRDRVSKAHFLARMAESCFELALKKRTEDDKDHYANKRLK
LAGDLMEDLFRVSFNRLARDIKYQLERANMRNRELNVATIVRADVLTDRLQHPLATGNWVGGRTGVSQLLDRNDYISTLS
HLRRVISPLSRAQPHFEARDLHATQWGRLCPSETPEGPNCGLVKNFAEMVELSTGTEEIENMKKILYDLNVEKVVVKVPE
IPAKIKEILLDEFGEEIVEEFEETPEPETKFKDDDFYDYSDMDDSGSMYGD

Sequences:

>Translated_531_residues
MQHHIDSFNKFIDYGLQKIIDEQRIIETDIEDTYLKLGKIRVEHPVVKEADGAIEKLYPNEGRLRNLSYSGPLYLEMSVV
KDGIESEAMEAKIGQLPVMVKSKICNLLGLSEVEKVRYGEDPLDPGGYFIIGGTERVVMTLEDLAPNKILVEFGERYGDE
IEVAKVFSQKRGYRALVIVERGRKSLLEVSFPSISGRVYFITLMRALGIVTDEDIVNAVSSDPEIIKFMLENLEEAEVET
QEEAIEKIGARVAAGQAKEYQIKRANYVIDRYLFPHLGNDIRDRVSKAHFLARMAESCFELALKKRTEDDKDHYANKRLK
LAGDLMEDLFRVSFNRLARDIKYQLERANMRNRELNVATIVRADVLTDRLQHPLATGNWVGGRTGVSQLLDRNDYISTLS
HLRRVISPLSRAQPHFEARDLHATQWGRLCPSETPEGPNCGLVKNFAEMVELSTGTEEIENMKKILYDLNVEKVVVKVPE
IPAKIKEILLDEFGEEIVEEFEETPEPETKFKDDDFYDYSDMDDSGSMYGD
>Mature_531_residues
MQHHIDSFNKFIDYGLQKIIDEQRIIETDIEDTYLKLGKIRVEHPVVKEADGAIEKLYPNEGRLRNLSYSGPLYLEMSVV
KDGIESEAMEAKIGQLPVMVKSKICNLLGLSEVEKVRYGEDPLDPGGYFIIGGTERVVMTLEDLAPNKILVEFGERYGDE
IEVAKVFSQKRGYRALVIVERGRKSLLEVSFPSISGRVYFITLMRALGIVTDEDIVNAVSSDPEIIKFMLENLEEAEVET
QEEAIEKIGARVAAGQAKEYQIKRANYVIDRYLFPHLGNDIRDRVSKAHFLARMAESCFELALKKRTEDDKDHYANKRLK
LAGDLMEDLFRVSFNRLARDIKYQLERANMRNRELNVATIVRADVLTDRLQHPLATGNWVGGRTGVSQLLDRNDYISTLS
HLRRVISPLSRAQPHFEARDLHATQWGRLCPSETPEGPNCGLVKNFAEMVELSTGTEEIENMKKILYDLNVEKVVVKVPE
IPAKIKEILLDEFGEEIVEEFEETPEPETKFKDDDFYDYSDMDDSGSMYGD

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=503, Percent_Identity=35.3876739562624, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI238908503, Length=490, Percent_Identity=30.8163265306122, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI238908505, Length=456, Percent_Identity=30.7017543859649, Blast_Score=216, Evalue=5e-56,
Organism=Homo sapiens, GI33469941, Length=515, Percent_Identity=24.2718446601942, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1790419, Length=144, Percent_Identity=34.0277777777778, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552304, Length=496, Percent_Identity=34.4758064516129, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI25144348, Length=492, Percent_Identity=29.0650406504065, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17506623, Length=529, Percent_Identity=23.2514177693762, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324725, Length=510, Percent_Identity=34.7058823529412, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6324781, Length=493, Percent_Identity=32.0486815415822, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6325267, Length=533, Percent_Identity=23.4521575984991, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17136444, Length=498, Percent_Identity=34.9397590361446, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI17647877, Length=474, Percent_Identity=30.8016877637131, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17136446, Length=491, Percent_Identity=21.7922606924644, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 60585; Mature: 60585

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHHIDSFNKFIDYGLQKIIDEQRIIETDIEDTYLKLGKIRVEHPVVKEADGAIEKLYPN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCHHHCCCHHHHHCCC
EGRLRNLSYSGPLYLEMSVVKDGIESEAMEAKIGQLPVMVKSKICNLLGLSEVEKVRYGE
CCCEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCC
DPLDPGGYFIIGGTERVVMTLEDLAPNKILVEFGERYGDEIEVAKVFSQKRGYRALVIVE
CCCCCCCEEEECCCCEEEEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEE
RGRKSLLEVSFPSISGRVYFITLMRALGIVTDEDIVNAVSSDPEIIKFMLENLEEAEVET
CCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QEEAIEKIGARVAAGQAKEYQIKRANYVIDRYLFPHLGNDIRDRVSKAHFLARMAESCFE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LALKKRTEDDKDHYANKRLKLAGDLMEDLFRVSFNRLARDIKYQLERANMRNRELNVATI
HHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VRADVLTDRLQHPLATGNWVGGRTGVSQLLDRNDYISTLSHLRRVISPLSRAQPHFEARD
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LHATQWGRLCPSETPEGPNCGLVKNFAEMVELSTGTEEIENMKKILYDLNVEKVVVKVPE
CCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHEEEECCC
IPAKIKEILLDEFGEEIVEEFEETPEPETKFKDDDFYDYSDMDDSGSMYGD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQHHIDSFNKFIDYGLQKIIDEQRIIETDIEDTYLKLGKIRVEHPVVKEADGAIEKLYPN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCHHHCCCHHHHHCCC
EGRLRNLSYSGPLYLEMSVVKDGIESEAMEAKIGQLPVMVKSKICNLLGLSEVEKVRYGE
CCCEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCC
DPLDPGGYFIIGGTERVVMTLEDLAPNKILVEFGERYGDEIEVAKVFSQKRGYRALVIVE
CCCCCCCEEEECCCCEEEEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEE
RGRKSLLEVSFPSISGRVYFITLMRALGIVTDEDIVNAVSSDPEIIKFMLENLEEAEVET
CCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QEEAIEKIGARVAAGQAKEYQIKRANYVIDRYLFPHLGNDIRDRVSKAHFLARMAESCFE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LALKKRTEDDKDHYANKRLKLAGDLMEDLFRVSFNRLARDIKYQLERANMRNRELNVATI
HHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VRADVLTDRLQHPLATGNWVGGRTGVSQLLDRNDYISTLSHLRRVISPLSRAQPHFEARD
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LHATQWGRLCPSETPEGPNCGLVKNFAEMVELSTGTEEIENMKKILYDLNVEKVVVKVPE
CCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHEEEECCC
IPAKIKEILLDEFGEEIVEEFEETPEPETKFKDDDFYDYSDMDDSGSMYGD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2495365; 11016950 [H]