Click here to switch to the map view.

The map label for this gene is rpoB1 [H]

Identifier: 21228374

GI number: 21228374

Start: 2698917

End: 2700731

Strand: Reverse

Name: rpoB1 [H]

Synonym: MM_2272

Alternate gene names: 21228374

Gene position: 2700731-2698917 (Counterclockwise)

Preceding gene: 21228375

Following gene: 21228373

Centisome position: 65.93

GC content: 48.82

Gene sequence:

>1815_bases
ATGGCTAAAGCAAAAGTATTCATAAACGGAGAGCTCGTCGGGACTCATGACAACCCTGCGGAACTCGTAGAAGAGCTCAG
AAAAATGAGGCGGCAGGGATTCATATCCAGACAGGTCAATATAGCTCTAAATGACAGTACCAGGGAAGTAATTATAAATT
CCGATATGGGCAGGGCAAGAAGACCTCTTCTGGTTGTGGAAAATGGTTCTCCCAAAGTAACCGAAGAGCATGTACAGAAA
CTGAAGAACAAGGAAATCAGTTTTGACGACCTTGTGAAAGAAGGCTGTGTGGAGTATCTGGATGCCGAAGAAGAGGAAAA
CGCATACATTGCCCTCTATGAGTCCGACATTACTGACAGGCATACCCATATGGAGATCGACCCCGAAATGATGCTCGGCA
TCTGTACCGGGATGGTTCCTTACCCGGAACACAACGCATCTCCGCGTAATACCATGGGTGCAGCCATGATCAAACAGTGT
ATCGGAGTTTCCACAGCAAACCAGAAGTTAAGGCCGGATACCCGTGCCCACGTCCTTCACTATCCTCAGAGGGCACTCAC
CAGAACAAGAACATCCGAAGCAATAGGCTTCGATGACAGACCTGCAGGCCAGAACTTCGTAGTAGCAATTCTGTCTTATG
AAGGATATAATATTGAAGACGCCCTGATCTTCAACAGGGGTTCAATCGATAGAGGGCTCGGGAGAAGTCATTTCCTCAGG
ACTTTTGAGGGTGAAGAAAGAAGGTACCCCGGTGGACAGGAAGATAAATTCGAAATTCCTGACTCCGAATACCGCGGAGC
CCGCAGTGCAGAAGCATATGCAAACCTTGACGTGGATGGGCTTGTGAACCCTGAGACCCCTGTAGGCCCCAATGACGTGC
TTATAGGAAAGACCAGCCCTCCCAGGTTCCTTGAAGAGCCTTCCGATTTCGGGATTGCAGTAGAACAGAGAAGAGAAAGC
TCTGTTACCATGCGGTCTAACGAAACAGGGATTGTGGACACAGTAATTCTTACAGAATCCATTAACGGAACCCGCCTTGC
CAAAGTAAAGGTAAGGGATGAGAGGATCCCGGACATAGGGGATAAGTTCGCTTCCAGGCACGGGCAAAAAGGAGTTATCG
GCTTAAAAGTCCCTATTGCGGACATGCCTTTTACGGAATCAGGCCTCACCCCTGATCTGATGATTAACCCGCACGCAATT
CCTTCACGTATGACTGTAGGACACGTCCTGGAAATGATCGGCGGGAAAGTGGGCTCAATGGAAGGCAGAAGAGTAAATGC
AACAGCCTTCTCAGGAGAAAAAGAGGACGATTTAAGAGAAGCCCTGAAAAAACACGGCTTTGCCCACACAGGAAAAGAGG
TTTTCTATGACGGAGCAACAGGCAGGATGATCCCTGCAGATGTCTTCGTTGGTGTAATCCTTTACCAGAAACTTCACCAC
ATGGTTACTTCCAAGATGCACGCAAGGTCTCGCGGACCCGTACAGGTGCTTACCCGTCAGCCGACTGAGGGTCGTGCAAG
GGAAGGTGGTCTCAGGTTCGGGGAAATGGAGCGTGATGTTCTGGTAGGGCACGGAGCAGCCATGTCCTTAAAAGAAAGAT
TGCTTGACGAGTCTGACAAAGTGGTTGAACTTGTCTGCTCGCACTGTGGAATGGTTGCAACATACGACAAGCAGAGGAAC
GTAGCTTTCTGTTCGGCCTGCGGTGCTGACACTGATATATATCCTGTAGAGATGAGCTATGCTTTCAAGCTGTTACTTGA
CGAAATTAAGTCTCTTGGAGTCGCTCCCAGGCTTAACCTTGAAGATGCTGTATAA

Upstream 100 bases:

>100_bases
CTCCTGAACCCGAAACCAAATTCAAAGATGATGATTTCTACGATTATTCAGATATGGACGACTCTGGATCTATGTATGGT
GACTGAGGGGTTGATGTCAG

Downstream 100 bases:

>100_bases
GTGAGGTGGACATATGGCAAACGTACCTCCAATTCCTAAAAGAATTTCTTCTATCAAATTCGGCTTAATGTCCCCAAAAG
AAATCCGTAAGATGAGTGCA

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MAKAKVFINGELVGTHDNPAELVEELRKMRRQGFISRQVNIALNDSTREVIINSDMGRARRPLLVVENGSPKVTEEHVQK
LKNKEISFDDLVKEGCVEYLDAEEEENAYIALYESDITDRHTHMEIDPEMMLGICTGMVPYPEHNASPRNTMGAAMIKQC
IGVSTANQKLRPDTRAHVLHYPQRALTRTRTSEAIGFDDRPAGQNFVVAILSYEGYNIEDALIFNRGSIDRGLGRSHFLR
TFEGEERRYPGGQEDKFEIPDSEYRGARSAEAYANLDVDGLVNPETPVGPNDVLIGKTSPPRFLEEPSDFGIAVEQRRES
SVTMRSNETGIVDTVILTESINGTRLAKVKVRDERIPDIGDKFASRHGQKGVIGLKVPIADMPFTESGLTPDLMINPHAI
PSRMTVGHVLEMIGGKVGSMEGRRVNATAFSGEKEDDLREALKKHGFAHTGKEVFYDGATGRMIPADVFVGVILYQKLHH
MVTSKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLVGHGAAMSLKERLLDESDKVVELVCSHCGMVATYDKQRN
VAFCSACGADTDIYPVEMSYAFKLLLDEIKSLGVAPRLNLEDAV

Sequences:

>Translated_604_residues
MAKAKVFINGELVGTHDNPAELVEELRKMRRQGFISRQVNIALNDSTREVIINSDMGRARRPLLVVENGSPKVTEEHVQK
LKNKEISFDDLVKEGCVEYLDAEEEENAYIALYESDITDRHTHMEIDPEMMLGICTGMVPYPEHNASPRNTMGAAMIKQC
IGVSTANQKLRPDTRAHVLHYPQRALTRTRTSEAIGFDDRPAGQNFVVAILSYEGYNIEDALIFNRGSIDRGLGRSHFLR
TFEGEERRYPGGQEDKFEIPDSEYRGARSAEAYANLDVDGLVNPETPVGPNDVLIGKTSPPRFLEEPSDFGIAVEQRRES
SVTMRSNETGIVDTVILTESINGTRLAKVKVRDERIPDIGDKFASRHGQKGVIGLKVPIADMPFTESGLTPDLMINPHAI
PSRMTVGHVLEMIGGKVGSMEGRRVNATAFSGEKEDDLREALKKHGFAHTGKEVFYDGATGRMIPADVFVGVILYQKLHH
MVTSKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLVGHGAAMSLKERLLDESDKVVELVCSHCGMVATYDKQRN
VAFCSACGADTDIYPVEMSYAFKLLLDEIKSLGVAPRLNLEDAV
>Mature_603_residues
AKAKVFINGELVGTHDNPAELVEELRKMRRQGFISRQVNIALNDSTREVIINSDMGRARRPLLVVENGSPKVTEEHVQKL
KNKEISFDDLVKEGCVEYLDAEEEENAYIALYESDITDRHTHMEIDPEMMLGICTGMVPYPEHNASPRNTMGAAMIKQCI
GVSTANQKLRPDTRAHVLHYPQRALTRTRTSEAIGFDDRPAGQNFVVAILSYEGYNIEDALIFNRGSIDRGLGRSHFLRT
FEGEERRYPGGQEDKFEIPDSEYRGARSAEAYANLDVDGLVNPETPVGPNDVLIGKTSPPRFLEEPSDFGIAVEQRRESS
VTMRSNETGIVDTVILTESINGTRLAKVKVRDERIPDIGDKFASRHGQKGVIGLKVPIADMPFTESGLTPDLMINPHAIP
SRMTVGHVLEMIGGKVGSMEGRRVNATAFSGEKEDDLREALKKHGFAHTGKEVFYDGATGRMIPADVFVGVILYQKLHHM
VTSKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLVGHGAAMSLKERLLDESDKVVELVCSHCGMVATYDKQRNV
AFCSACGADTDIYPVEMSYAFKLLLDEIKSLGVAPRLNLEDAV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4505941, Length=611, Percent_Identity=45.3355155482815, Blast_Score=526, Evalue=1e-149,
Organism=Homo sapiens, GI238908505, Length=598, Percent_Identity=42.9765886287625, Blast_Score=501, Evalue=1e-141,
Organism=Homo sapiens, GI238908503, Length=598, Percent_Identity=42.9765886287625, Blast_Score=501, Evalue=1e-141,
Organism=Homo sapiens, GI212286172, Length=523, Percent_Identity=32.1223709369025, Blast_Score=285, Evalue=1e-76,
Organism=Homo sapiens, GI33469941, Length=523, Percent_Identity=32.1223709369025, Blast_Score=285, Evalue=1e-76,
Organism=Escherichia coli, GI1790419, Length=312, Percent_Identity=32.0512820512821, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17552304, Length=607, Percent_Identity=43.8220757825371, Blast_Score=524, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI25144348, Length=604, Percent_Identity=41.5562913907285, Blast_Score=501, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17506623, Length=533, Percent_Identity=30.9568480300188, Blast_Score=285, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324725, Length=639, Percent_Identity=42.8794992175274, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324781, Length=604, Percent_Identity=43.5430463576159, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6325267, Length=480, Percent_Identity=36.0416666666667, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17136444, Length=609, Percent_Identity=45.6486042692939, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI17647877, Length=597, Percent_Identity=42.8810720268007, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI17136446, Length=620, Percent_Identity=31.7741935483871, Blast_Score=291, Evalue=7e-79,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 67024; Mature: 66893

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKVFINGELVGTHDNPAELVEELRKMRRQGFISRQVNIALNDSTREVIINSDMGRAR
CCCEEEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCC
RPLLVVENGSPKVTEEHVQKLKNKEISFDDLVKEGCVEYLDAEEEENAYIALYESDITDR
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTHMEIDPEMMLGICTGMVPYPEHNASPRNTMGAAMIKQCIGVSTANQKLRPDTRAHVLH
CCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
YPQRALTRTRTSEAIGFDDRPAGQNFVVAILSYEGYNIEDALIFNRGSIDRGLGRSHFLR
CCHHHHHHHHCHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHH
TFEGEERRYPGGQEDKFEIPDSEYRGARSAEAYANLDVDGLVNPETPVGPNDVLIGKTSP
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCC
PRFLEEPSDFGIAVEQRRESSVTMRSNETGIVDTVILTESINGTRLAKVKVRDERIPDIG
CHHHCCCCCCCEEHECCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHH
DKFASRHGQKGVIGLKVPIADMPFTESGLTPDLMINPHAIPSRMTVGHVLEMIGGKVGSM
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCC
EGRRVNATAFSGEKEDDLREALKKHGFAHTGKEVFYDGATGRMIPADVFVGVILYQKLHH
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
MVTSKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLVGHGAAMSLKERLLDESDK
HHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHH
VVELVCSHCGMVATYDKQRNVAFCSACGADTDIYPVEMSYAFKLLLDEIKSLGVAPRLNL
HHHHHHHHCCEEEEECCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC
EDAV
CCCC
>Mature Secondary Structure 
AKAKVFINGELVGTHDNPAELVEELRKMRRQGFISRQVNIALNDSTREVIINSDMGRAR
CCEEEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCC
RPLLVVENGSPKVTEEHVQKLKNKEISFDDLVKEGCVEYLDAEEEENAYIALYESDITDR
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTHMEIDPEMMLGICTGMVPYPEHNASPRNTMGAAMIKQCIGVSTANQKLRPDTRAHVLH
CCEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
YPQRALTRTRTSEAIGFDDRPAGQNFVVAILSYEGYNIEDALIFNRGSIDRGLGRSHFLR
CCHHHHHHHHCHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHH
TFEGEERRYPGGQEDKFEIPDSEYRGARSAEAYANLDVDGLVNPETPVGPNDVLIGKTSP
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEECCCC
PRFLEEPSDFGIAVEQRRESSVTMRSNETGIVDTVILTESINGTRLAKVKVRDERIPDIG
CHHHCCCCCCCEEHECCCCCCEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCHH
DKFASRHGQKGVIGLKVPIADMPFTESGLTPDLMINPHAIPSRMTVGHVLEMIGGKVGSM
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCC
EGRRVNATAFSGEKEDDLREALKKHGFAHTGKEVFYDGATGRMIPADVFVGVILYQKLHH
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
MVTSKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLVGHGAAMSLKERLLDESDK
HHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHH
VVELVCSHCGMVATYDKQRNVAFCSACGADTDIYPVEMSYAFKLLLDEIKSLGVAPRLNL
HHHHHHHHCCEEEEECCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC
EDAV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]