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The map label for this gene is yfeJ [H]

Identifier: 21228282

GI number: 21228282

Start: 2585728

End: 2586450

Strand: Reverse

Name: yfeJ [H]

Synonym: MM_2180

Alternate gene names: 21228282

Gene position: 2586450-2585728 (Counterclockwise)

Preceding gene: 21228285

Following gene: 21228280

Centisome position: 63.14

GC content: 45.23

Gene sequence:

>723_bases
ATGAAAATCCACTGCCTCCAGCACCTGAAAAACGAGACCCTTGGAAACATAGGAACCTGGGTGACTCTGAAAGGGCACAG
CCTTACAAAAACCCTGCCTTGTGAAAAATCTGCTTTTCCTGATCCCGCAGAATTTGATATGCTTCTGATAATGGGCGGCA
CTATGAGTGTTTATCAGGAGAAAGAGTACACCTGGCTGAAACCTGAAAAAGAGTTTGTAAAAAAAGTAATAGGCATGGGT
AAACCTGTACTGGGAAGCTGTTTCGGGGCACAGTTGATTGCAGATGTTCTGGGAGGAAAAGTTACCAGAAACCGGTTCAA
GGAGATAGGCTGGCACAGGGTAAAAGCTGTTGCAGAGGAAAATTCGAATAATGAAGGAAAAATAATCTCAGGGCTTCCTG
CAGGCTTATTTCCCGAGTTTACCGCATTTATGTGGCATGGTGACACTTTCGAAATCCCAGAAGGTGCGGTAAGGCTTTTC
AAAAGCGAAGCCTGCCAGAACCAGGGTTTCATTTATGGGGATAATGTCCTCGGGCTGCAGTTCCACCCTGAAACCGACAG
GCAGTGGATATGTAACCTGATAGAAGATTCGGGACATGAGCTTGTTGAAGGAGAATATATCCAGCCGGGAGAAGAAATGC
TCAAAAACGAGCACTTTCTTGAAGGCTCCCGGAAAATTGCTTTTTCCCTCATGGACTGGTTTGAGAAAAAATGTGGAAAT
TGA

Upstream 100 bases:

>100_bases
AATGTCTTTGATTACCGGGAAAACCTGATTTTGAAAAAATAAAACAGTACTGAAATCGAAACTAAAGTGAAACTGAAATC
GAACCCAAAGTGAAAACCCC

Downstream 100 bases:

>100_bases
ATTGAAAGTGGATAGCCACAAGTAAAAATCTTTAAACCTTCAGGCAGGAGCGGATATTAATTGCTCCGTCCTTAGATAAA
TTCCCACGGTTCCTTTTTAA

Product: GMP synthase

Products: AMP; diphosphate; GMP; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIHCLQHLKNETLGNIGTWVTLKGHSLTKTLPCEKSAFPDPAEFDMLLIMGGTMSVYQEKEYTWLKPEKEFVKKVIGMG
KPVLGSCFGAQLIADVLGGKVTRNRFKEIGWHRVKAVAEENSNNEGKIISGLPAGLFPEFTAFMWHGDTFEIPEGAVRLF
KSEACQNQGFIYGDNVLGLQFHPETDRQWICNLIEDSGHELVEGEYIQPGEEMLKNEHFLEGSRKIAFSLMDWFEKKCGN

Sequences:

>Translated_240_residues
MKIHCLQHLKNETLGNIGTWVTLKGHSLTKTLPCEKSAFPDPAEFDMLLIMGGTMSVYQEKEYTWLKPEKEFVKKVIGMG
KPVLGSCFGAQLIADVLGGKVTRNRFKEIGWHRVKAVAEENSNNEGKIISGLPAGLFPEFTAFMWHGDTFEIPEGAVRLF
KSEACQNQGFIYGDNVLGLQFHPETDRQWICNLIEDSGHELVEGEYIQPGEEMLKNEHFLEGSRKIAFSLMDWFEKKCGN
>Mature_240_residues
MKIHCLQHLKNETLGNIGTWVTLKGHSLTKTLPCEKSAFPDPAEFDMLLIMGGTMSVYQEKEYTWLKPEKEFVKKVIGMG
KPVLGSCFGAQLIADVLGGKVTRNRFKEIGWHRVKAVAEENSNNEGKIISGLPAGLFPEFTAFMWHGDTFEIPEGAVRLF
KSEACQNQGFIYGDNVLGLQFHPETDRQWICNLIEDSGHELVEGEYIQPGEEMLKNEHFLEGSRKIAFSLMDWFEKKCGN

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2

Molecular weight: Translated: 27116; Mature: 27116

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHCLQHLKNETLGNIGTWVTLKGHSLTKTLPCEKSAFPDPAEFDMLLIMGGTMSVYQE
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHC
KEYTWLKPEKEFVKKVIGMGKPVLGSCFGAQLIADVLGGKVTRNRFKEIGWHRVKAVAEE
CCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
NSNNEGKIISGLPAGLFPEFTAFMWHGDTFEIPEGAVRLFKSEACQNQGFIYGDNVLGLQ
CCCCCCCEEECCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEEE
FHPETDRQWICNLIEDSGHELVEGEYIQPGEEMLKNEHFLEGSRKIAFSLMDWFEKKCGN
ECCCCCHHHHHHHHHCCCCCEECCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIHCLQHLKNETLGNIGTWVTLKGHSLTKTLPCEKSAFPDPAEFDMLLIMGGTMSVYQE
CCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHC
KEYTWLKPEKEFVKKVIGMGKPVLGSCFGAQLIADVLGGKVTRNRFKEIGWHRVKAVAEE
CCCCEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
NSNNEGKIISGLPAGLFPEFTAFMWHGDTFEIPEGAVRLFKSEACQNQGFIYGDNVLGLQ
CCCCCCCEEECCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEEE
FHPETDRQWICNLIEDSGHELVEGEYIQPGEEMLKNEHFLEGSRKIAFSLMDWFEKKCGN
ECCCCCHHHHHHHHHCCCCCEECCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 1 {NH4+}} 0.42 {6-thioXMP}} 0.35 {8-azaXMP}} 0.53 {ATP}} [C]

Substrates: ATP; xanthosine 5'-phosphate; L-glutamine; H2O

Specific reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609 [H]