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The map label for this gene is rfcS [H]

Identifier: 21227528

GI number: 21227528

Start: 1708793

End: 1709818

Strand: Direct

Name: rfcS [H]

Synonym: MM_1426

Alternate gene names: 21227528

Gene position: 1708793-1709818 (Clockwise)

Preceding gene: 21227527

Following gene: 21227529

Centisome position: 41.72

GC content: 46.59

Gene sequence:

>1026_bases
ATGCAGGATCTCTGGACTCTAAAATACAGGGCAGACACTCTTGAAAGGCTGCTCGGTAACGAACATGCCGTGAAAACGCT
TTCCGAACTGGTCCAGTCCAGGACCCTGCCCCATCTCATTTTTTACGGCCCTGAAAATTCGGGAAAAACAACGGCTGCTC
TTGCTCTTGCTAGGCAGCTCTATGGAGATACATGGAAAAACAACTTTGCATATTTTAACGCTTCGGATTTTTTCAACCAG
GGAAAACGTTATCTTGTGCGGGACAGGCGTTTTGTCCGTTTTATAGGGACTGATGACCCGAAAAAAATCTACAAAAGCGT
GATCGATATTTTTAAGGAAATCATTAACGAGTATGCCGGAATGGCGCCTCTAGACGCTGATTATAAGCTTATTTATATCG
ATAATGCGGAATCTCTTAGTTCGGATGCACAGCACGCCCTGAGGCGAATCATGGAAAAGTACAGTGCAACCTGCAGGTTT
ATCCTTTCCACTACAAGACCTTCAAAACTCATTTCTCCTCTCCGCTCAAGAGGCCTTCAGGTCTTTTTTGCCTATGTTTC
GGATTCCCTCGTGAAGCCCCATCTCGAGAGGATCGCCCTTTCCGAGGACCTGGAGCTTTCAGATGGGGCGCTTGATGCGA
TCCTTTATCTTTCTAAGGGAAATATTGCAAGAGCTGTTCAGACTCTGCAGCTCGCTGCCCTTAAAGCTGAAAGCGCTGAA
ATTACGGAAGAGATCGTCTTTGAAGCTACCATGAAAGGCAGGGACGAAACTGTAGATTCGTTGCTTGAGGCTGCACTTGC
AGGCGACTTCCCCAGAGGGCGCCAGCTCATAGATGAAATGATAATTGAAAAAGGGCTTTCAGGGACTGATATCCTCGAAG
GTCTTTCCGAAGCCCTTATCGATTCTGGAGAACGCGATAAGGATGTCGCACGTCTTATTATAGAAATATCAGATGCCGAT
TCAAGGCTTACAGATGCCACCAGTGAGAGAATCCAGCTCGAAAAGCTGATTTCCACATTCTCCTGA

Upstream 100 bases:

>100_bases
TGCAGATGAAAATGGAAACGGAAGCATAACAGAGTAAATATTGAGAAAAAAGTAAAGGCACTGAAGTGACATTTAAATGC
GGGAAAAATAGGTGGAATCA

Downstream 100 bases:

>100_bases
ATTTTTTCAAAAATTAAATCACCTTTTCAAAACTGGGCTCTGTAGAAATCCTATTTGATGTAAATTATTTCTACAACTTT
TTATTTTGTGCTACTTTTGA

Product: replication factor C small subunit 2

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQDLWTLKYRADTLERLLGNEHAVKTLSELVQSRTLPHLIFYGPENSGKTTAALALARQLYGDTWKNNFAYFNASDFFNQ
GKRYLVRDRRFVRFIGTDDPKKIYKSVIDIFKEIINEYAGMAPLDADYKLIYIDNAESLSSDAQHALRRIMEKYSATCRF
ILSTTRPSKLISPLRSRGLQVFFAYVSDSLVKPHLERIALSEDLELSDGALDAILYLSKGNIARAVQTLQLAALKAESAE
ITEEIVFEATMKGRDETVDSLLEAALAGDFPRGRQLIDEMIIEKGLSGTDILEGLSEALIDSGERDKDVARLIIEISDAD
SRLTDATSERIQLEKLISTFS

Sequences:

>Translated_341_residues
MQDLWTLKYRADTLERLLGNEHAVKTLSELVQSRTLPHLIFYGPENSGKTTAALALARQLYGDTWKNNFAYFNASDFFNQ
GKRYLVRDRRFVRFIGTDDPKKIYKSVIDIFKEIINEYAGMAPLDADYKLIYIDNAESLSSDAQHALRRIMEKYSATCRF
ILSTTRPSKLISPLRSRGLQVFFAYVSDSLVKPHLERIALSEDLELSDGALDAILYLSKGNIARAVQTLQLAALKAESAE
ITEEIVFEATMKGRDETVDSLLEAALAGDFPRGRQLIDEMIIEKGLSGTDILEGLSEALIDSGERDKDVARLIIEISDAD
SRLTDATSERIQLEKLISTFS
>Mature_341_residues
MQDLWTLKYRADTLERLLGNEHAVKTLSELVQSRTLPHLIFYGPENSGKTTAALALARQLYGDTWKNNFAYFNASDFFNQ
GKRYLVRDRRFVRFIGTDDPKKIYKSVIDIFKEIINEYAGMAPLDADYKLIYIDNAESLSSDAQHALRRIMEKYSATCRF
ILSTTRPSKLISPLRSRGLQVFFAYVSDSLVKPHLERIALSEDLELSDGALDAILYLSKGNIARAVQTLQLAALKAESAE
ITEEIVFEATMKGRDETVDSLLEAALAGDFPRGRQLIDEMIIEKGLSGTDILEGLSEALIDSGERDKDVARLIIEISDAD
SRLTDATSERIQLEKLISTFS

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI6677723, Length=340, Percent_Identity=30.5882352941176, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI194306571, Length=337, Percent_Identity=30.5637982195846, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI194306567, Length=337, Percent_Identity=30.5637982195846, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI4506491, Length=345, Percent_Identity=27.536231884058, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI31881687, Length=345, Percent_Identity=27.536231884058, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI31563534, Length=228, Percent_Identity=30.2631578947368, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI194306569, Length=234, Percent_Identity=30.3418803418803, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71986063, Length=330, Percent_Identity=28.7878787878788, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17541988, Length=341, Percent_Identity=27.2727272727273, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17554730, Length=300, Percent_Identity=28.3333333333333, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17541990, Length=254, Percent_Identity=27.9527559055118, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322528, Length=348, Percent_Identity=31.0344827586207, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324039, Length=338, Percent_Identity=26.6272189349112, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324478, Length=296, Percent_Identity=27.3648648648649, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319562, Length=282, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI19921076, Length=339, Percent_Identity=28.3185840707965, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI18859927, Length=334, Percent_Identity=28.1437125748503, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17647857, Length=293, Percent_Identity=27.6450511945392, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19921136, Length=345, Percent_Identity=25.2173913043478, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 38349; Mature: 38349

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLWTLKYRADTLERLLGNEHAVKTLSELVQSRTLPHLIFYGPENSGKTTAALALARQL
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
YGDTWKNNFAYFNASDFFNQGKRYLVRDRRFVRFIGTDDPKKIYKSVIDIFKEIINEYAG
HCCCCCCCCEEECCHHHHHCCCHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHCC
MAPLDADYKLIYIDNAESLSSDAQHALRRIMEKYSATCRFILSTTRPSKLISPLRSRGLQ
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHH
VFFAYVSDSLVKPHLERIALSEDLELSDGALDAILYLSKGNIARAVQTLQLAALKAESAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHH
ITEEIVFEATMKGRDETVDSLLEAALAGDFPRGRQLIDEMIIEKGLSGTDILEGLSEALI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DSGERDKDVARLIIEISDADSRLTDATSERIQLEKLISTFS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDLWTLKYRADTLERLLGNEHAVKTLSELVQSRTLPHLIFYGPENSGKTTAALALARQL
CCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
YGDTWKNNFAYFNASDFFNQGKRYLVRDRRFVRFIGTDDPKKIYKSVIDIFKEIINEYAG
HCCCCCCCCEEECCHHHHHCCCHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHCC
MAPLDADYKLIYIDNAESLSSDAQHALRRIMEKYSATCRFILSTTRPSKLISPLRSRGLQ
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHH
VFFAYVSDSLVKPHLERIALSEDLELSDGALDAILYLSKGNIARAVQTLQLAALKAESAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCHHHHHHHHHHHHHHHHHHH
ITEEIVFEATMKGRDETVDSLLEAALAGDFPRGRQLIDEMIIEKGLSGTDILEGLSEALI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
DSGERDKDVARLIIEISDADSRLTDATSERIQLEKLISTFS
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA