Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 21227382

GI number: 21227382

Start: 1516086

End: 1516703

Strand: Reverse

Name: nth [H]

Synonym: MM_1280

Alternate gene names: 21227382

Gene position: 1516703-1516086 (Counterclockwise)

Preceding gene: 21227383

Following gene: 21227381

Centisome position: 37.03

GC content: 44.98

Gene sequence:

>618_bases
ATGGACACTGACGAACTCATGCGGAGACTTTTCGAACTTTATCCCGATGGCTGCACTGTAGATGTACGGGAGCCTTTTTT
TGCATTGATTTCCACTGTCATGTCACACAGAACCCGTGACGATGTGACCTACCCTGCAGCAAAGAAGCTTTTTGAAAGGT
TTTCAACCCCTGAGGAAATGGTCGAAGCTAACGTTGAGGATATTGAAGAGCTCATAAGAGACGTTGGTTTTTATCGGGTC
AAGGCAGGAAGGATAAAGGAAATATCCCGGATTTTACTGGAAGATTATAACGGCAAGGTCCCGGATGATATGGAGACTCT
CCTTAAACTCCCTGGAGTCGGCAGAAAAACAGCTAATTGTGTACTTGCGCATGCATTCCTTAAAGAAGATGCTCTTGCCG
TTGATACCCACGTCCACAGGATTTCTAACCGGCTAGGGCGGGTAGTAACAAAAAATCCCGAGGAAACGGAAATGGAATTA
AAAAAGCTCCTGCCGCAAAAATACTGGAGACATGTAAATATTTTGCTTGTAAAATTCGGGCAAAATGTCTGCCGGCCCAT
ATCTCCCAGGTGCGGGATTTGCATCCTGAATGATATCTGCCCTAAAATTCTCCTTTGA

Upstream 100 bases:

>100_bases
AGTTGAGCTTTCTCTGGAAAATTTTTTTCTTTTTTAGCTGAGAAAACAATCACCTTTTTAATCTGGTATTTTTTATATCA
AAAGAATACTAATTCTGGGC

Downstream 100 bases:

>100_bases
TCAGGATTTCAATCCTCCTTTGATCAGGATTCCTTTACTATTTTTTCTGTGAAACTAATCTGTATAATCGAATTCATGAG
GCATTGTGCATGGATAGAAA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MDTDELMRRLFELYPDGCTVDVREPFFALISTVMSHRTRDDVTYPAAKKLFERFSTPEEMVEANVEDIEELIRDVGFYRV
KAGRIKEISRILLEDYNGKVPDDMETLLKLPGVGRKTANCVLAHAFLKEDALAVDTHVHRISNRLGRVVTKNPEETEMEL
KKLLPQKYWRHVNILLVKFGQNVCRPISPRCGICILNDICPKILL

Sequences:

>Translated_205_residues
MDTDELMRRLFELYPDGCTVDVREPFFALISTVMSHRTRDDVTYPAAKKLFERFSTPEEMVEANVEDIEELIRDVGFYRV
KAGRIKEISRILLEDYNGKVPDDMETLLKLPGVGRKTANCVLAHAFLKEDALAVDTHVHRISNRLGRVVTKNPEETEMEL
KKLLPQKYWRHVNILLVKFGQNVCRPISPRCGICILNDICPKILL
>Mature_205_residues
MDTDELMRRLFELYPDGCTVDVREPFFALISTVMSHRTRDDVTYPAAKKLFERFSTPEEMVEANVEDIEELIRDVGFYRV
KAGRIKEISRILLEDYNGKVPDDMETLLKLPGVGRKTANCVLAHAFLKEDALAVDTHVHRISNRLGRVVTKNPEETEMEL
KKLLPQKYWRHVNILLVKFGQNVCRPISPRCGICILNDICPKILL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=41.0112359550562, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI115298648, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI190358497, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI6912520, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298650, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298654, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI115298652, Length=191, Percent_Identity=26.1780104712042, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1787920, Length=197, Percent_Identity=39.5939086294416, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI1789331, Length=184, Percent_Identity=25, Blast_Score=70, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=42.6136363636364, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=33.695652173913, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319304, Length=170, Percent_Identity=33.5294117647059, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=43.2584269662921, Blast_Score=153, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23629; Mature: 23629

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTDELMRRLFELYPDGCTVDVREPFFALISTVMSHRTRDDVTYPAAKKLFERFSTPEEM
CCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHH
VEANVEDIEELIRDVGFYRVKAGRIKEISRILLEDYNGKVPDDMETLLKLPGVGRKTANC
HHCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHH
VLAHAFLKEDALAVDTHVHRISNRLGRVVTKNPEETEMELKKLLPQKYWRHVNILLVKFG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVCRPISPRCGICILNDICPKILL
HHHCCCCCCCCCEEEHHHHHHHHCC
>Mature Secondary Structure
MDTDELMRRLFELYPDGCTVDVREPFFALISTVMSHRTRDDVTYPAAKKLFERFSTPEEM
CCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHH
VEANVEDIEELIRDVGFYRVKAGRIKEISRILLEDYNGKVPDDMETLLKLPGVGRKTANC
HHCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHH
VLAHAFLKEDALAVDTHVHRISNRLGRVVTKNPEETEMELKKLLPQKYWRHVNILLVKFG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVCRPISPRCGICILNDICPKILL
HHHCCCCCCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]