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The map label for this gene is hyfB [H]

Identifier: 21227161

GI number: 21227161

Start: 1242273

End: 1244204

Strand: Direct

Name: hyfB [H]

Synonym: MM_1059

Alternate gene names: 21227161

Gene position: 1242273-1244204 (Clockwise)

Preceding gene: 21227160

Following gene: 21227162

Centisome position: 30.33

GC content: 47.36

Gene sequence:

>1932_bases
TTGCTCGAAACCTTAATGTATGGTGCAATCTCTGCCCTTGTATTCGGAACAGTCCTCCCTCTCCTGTACCGCAGTAAAAA
CATAAGAAAGGCTTCTTCTGGTCTTTCTCTTCTCTCATCAATCCTGCTTCTGGGCTTTGCGGGCATAATTCTTTACACCA
GAACAGAGCCTGTTTTTCCAGTTACCGGATTTCTTCCGGGCGCTGGTTTTACCCTGAGCGCAGACAGGCTTGCAGCCGGG
TTTATCCTGCTAATCGGAGCAGTAGTGCCCGGAGTTTCAATATATTCGGTAGAATACGTTGAACACGCTAAAAGCGAAGC
CAGAAAAAGCCTTCAGGCATCCCTTACAAATCTTTTCATCCTTGCCATGCTTATGGTCATTCTTGCAGGCAATATGGTAA
GTTTCCTGGTTTTCTGGGAAATAATGTCCCTTTCCTCTCTCCTCCTGGTTCTGCATGACTACTCTTCCGAGGAGAATAAA
AAAGCCGGCTTTTTCTACTTTGTAATGACCAATATAAGTACGGCTTTTCTTTTCGTGGGCTTCATAAGCCTCTTCAGGCT
GACGGGTTCTGCCGATTTCGGGCCTCTGGAATACCCTGCAGCAGATCTGGCTTTACCTTTCATCTTTCTGTTCATCGGAT
TTGGAGTCAAGGCAGGGCTTGTCCCTTTCCATAAATGGCTTCCCTATGCTCACCCTGCAGCCCCTTCAAGTGTTTCAGCC
CTGATGTCAGGAGTCATGCTGAAAGTTGCAGTTTACGGCTTCCTGCGTTTTCTGCTATCGGTATCTGCTCCCGAACTCTG
GTGGGGAGTGCTTATCCTTACAGCAGGCAGCATTTCAGCCCTTTTCGGGGTAATCTATGCCCTTAAAGAAAATGACATAA
AAAGGATGCTCGCCTACAGCAGCATTGAAAACATAGGAATTATTTTCACAGGAATCGGGCTGTATGCAGTATTTAAAGTT
GAGGGGCTTGAATCCCTTGCCCTCTTAAGCCTTGTAGGGGCCTGCTTCCATGCCTTTAACCATGCTGTCTTCAAAAGCCT
GCTCTTCCTCTGTGCAGGCTCAGTGGTCCATGCTACAGGCACGAGGAAAATCGAAGCATTTGGGGGGCTTGTAAAAAGCA
TGCCTGTAACCTCTGCCCTTTTCCTGATAGGTTCGGTATCTATTGCAGCCATGCCGCCAACAAATGGTTTTGCCGGCGAG
CTCCTGCTTTATCAGGCTTTCTTCCAGTCTTTTGCCGTAACAGATCCTCTACTGAACATATTTCTGATAATCGCCCTCTC
AAGCTTTGCCCTTACCGGAGCCCTTGCAGCTGCCCTTTTCGTAAAACTTTTCGGGATCACCTGCCTTGCAATCCCGCGCT
CTGAGAAGAGCAGGCTTGCAGAAGAAGTCCAGAAACCAATGCTTGTAGGGCCTGCAGTGCCGGCAGCTTTCTGTATACTG
GGCGGGTTGTTTTCAAAGCAGCTTCTATCTCTCGCAGGCTGGAAAATCGATTTTCCGGACCTTCTCTTGCTGGGGATTCT
TCTCGGGTTGATATACACAGCCCTTTTCGTAATAATCCGCTCAAAAGAAACGGGCCCTGCAAGGATTAGCGAGACATGGG
GATGCGGGATCCCGGCCCTTGCACCTTATATGGAATACAGCTCTGCAGGCTTTTCCGAGCCTCTTGTAATGATCTTCAAA
AGTATTTACAGGACAAAGATTGTGAACAGACCCGAGTACTTCGACGAAAAGGAAAGCCTCTTTAAGAACGGAAAGGTGGA
AATACGCCTGCTCCGCTTCTTTGAAGAATACCTTTACATCCCACCTGCGCGGGCAATTGATATTTTTTCAAAATATGTTT
CAAAACTGCAGAACGGAAAACTTGACAGTTATGTCCTTTACGCCTTTCTTGCAGTGATTGTACTAATTATAGCAACCGGA
GGCTTTGTATGA

Upstream 100 bases:

>100_bases
GCGGGACAGGGACTGAAAAAACAGGACTCTTTTCCCGAACTGGAAAACTGTTTTCAGCTTTTCTTTCAGCTGCCAGACCT
GGGTTTGAGGAGTGAATATA

Downstream 100 bases:

>100_bases
ACAGCTCGTTTCTGCTCTTCGCCCTCCTGAATCCCCTGTTTGCCCTGCTGGCAGCCCCTCTATACATGGCTCTGATTCGG
AAAGTGAAGGCATATGCCCA

Product: hydrogenase membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MLETLMYGAISALVFGTVLPLLYRSKNIRKASSGLSLLSSILLLGFAGIILYTRTEPVFPVTGFLPGAGFTLSADRLAAG
FILLIGAVVPGVSIYSVEYVEHAKSEARKSLQASLTNLFILAMLMVILAGNMVSFLVFWEIMSLSSLLLVLHDYSSEENK
KAGFFYFVMTNISTAFLFVGFISLFRLTGSADFGPLEYPAADLALPFIFLFIGFGVKAGLVPFHKWLPYAHPAAPSSVSA
LMSGVMLKVAVYGFLRFLLSVSAPELWWGVLILTAGSISALFGVIYALKENDIKRMLAYSSIENIGIIFTGIGLYAVFKV
EGLESLALLSLVGACFHAFNHAVFKSLLFLCAGSVVHATGTRKIEAFGGLVKSMPVTSALFLIGSVSIAAMPPTNGFAGE
LLLYQAFFQSFAVTDPLLNIFLIIALSSFALTGALAAALFVKLFGITCLAIPRSEKSRLAEEVQKPMLVGPAVPAAFCIL
GGLFSKQLLSLAGWKIDFPDLLLLGILLGLIYTALFVIIRSKETGPARISETWGCGIPALAPYMEYSSAGFSEPLVMIFK
SIYRTKIVNRPEYFDEKESLFKNGKVEIRLLRFFEEYLYIPPARAIDIFSKYVSKLQNGKLDSYVLYAFLAVIVLIIATG
GFV

Sequences:

>Translated_643_residues
MLETLMYGAISALVFGTVLPLLYRSKNIRKASSGLSLLSSILLLGFAGIILYTRTEPVFPVTGFLPGAGFTLSADRLAAG
FILLIGAVVPGVSIYSVEYVEHAKSEARKSLQASLTNLFILAMLMVILAGNMVSFLVFWEIMSLSSLLLVLHDYSSEENK
KAGFFYFVMTNISTAFLFVGFISLFRLTGSADFGPLEYPAADLALPFIFLFIGFGVKAGLVPFHKWLPYAHPAAPSSVSA
LMSGVMLKVAVYGFLRFLLSVSAPELWWGVLILTAGSISALFGVIYALKENDIKRMLAYSSIENIGIIFTGIGLYAVFKV
EGLESLALLSLVGACFHAFNHAVFKSLLFLCAGSVVHATGTRKIEAFGGLVKSMPVTSALFLIGSVSIAAMPPTNGFAGE
LLLYQAFFQSFAVTDPLLNIFLIIALSSFALTGALAAALFVKLFGITCLAIPRSEKSRLAEEVQKPMLVGPAVPAAFCIL
GGLFSKQLLSLAGWKIDFPDLLLLGILLGLIYTALFVIIRSKETGPARISETWGCGIPALAPYMEYSSAGFSEPLVMIFK
SIYRTKIVNRPEYFDEKESLFKNGKVEIRLLRFFEEYLYIPPARAIDIFSKYVSKLQNGKLDSYVLYAFLAVIVLIIATG
GFV
>Mature_643_residues
MLETLMYGAISALVFGTVLPLLYRSKNIRKASSGLSLLSSILLLGFAGIILYTRTEPVFPVTGFLPGAGFTLSADRLAAG
FILLIGAVVPGVSIYSVEYVEHAKSEARKSLQASLTNLFILAMLMVILAGNMVSFLVFWEIMSLSSLLLVLHDYSSEENK
KAGFFYFVMTNISTAFLFVGFISLFRLTGSADFGPLEYPAADLALPFIFLFIGFGVKAGLVPFHKWLPYAHPAAPSSVSA
LMSGVMLKVAVYGFLRFLLSVSAPELWWGVLILTAGSISALFGVIYALKENDIKRMLAYSSIENIGIIFTGIGLYAVFKV
EGLESLALLSLVGACFHAFNHAVFKSLLFLCAGSVVHATGTRKIEAFGGLVKSMPVTSALFLIGSVSIAAMPPTNGFAGE
LLLYQAFFQSFAVTDPLLNIFLIIALSSFALTGALAAALFVKLFGITCLAIPRSEKSRLAEEVQKPMLVGPAVPAAFCIL
GGLFSKQLLSLAGWKIDFPDLLLLGILLGLIYTALFVIIRSKETGPARISETWGCGIPALAPYMEYSSAGFSEPLVMIFK
SIYRTKIVNRPEYFDEKESLFKNGKVEIRLLRFFEEYLYIPPARAIDIFSKYVSKLQNGKLDSYVLYAFLAVIVLIIATG
GFV

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=288, Percent_Identity=31.25, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI251831116, Length=315, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=6e-16,
Organism=Escherichia coli, GI1788827, Length=627, Percent_Identity=33.8118022328549, Blast_Score=312, Evalue=4e-86,
Organism=Escherichia coli, GI2367154, Length=494, Percent_Identity=34.2105263157895, Blast_Score=222, Evalue=7e-59,
Organism=Escherichia coli, GI1788831, Length=366, Percent_Identity=31.1475409836066, Blast_Score=152, Evalue=8e-38,
Organism=Escherichia coli, GI1788614, Length=374, Percent_Identity=29.9465240641711, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788829, Length=388, Percent_Identity=28.0927835051546, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788613, Length=322, Percent_Identity=28.8819875776398, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693160, Length=282, Percent_Identity=26.9503546099291, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 69803; Mature: 69803

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETLMYGAISALVFGTVLPLLYRSKNIRKASSGLSLLSSILLLGFAGIILYTRTEPVFP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
VTGFLPGAGFTLSADRLAAGFILLIGAVVPGVSIYSVEYVEHAKSEARKSLQASLTNLFI
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LAMLMVILAGNMVSFLVFWEIMSLSSLLLVLHDYSSEENKKAGFFYFVMTNISTAFLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHH
FISLFRLTGSADFGPLEYPAADLALPFIFLFIGFGVKAGLVPFHKWLPYAHPAAPSSVSA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCCHHHHHCCCCCCCCCHHHHH
LMSGVMLKVAVYGFLRFLLSVSAPELWWGVLILTAGSISALFGVIYALKENDIKRMLAYS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
SIENIGIIFTGIGLYAVFKVEGLESLALLSLVGACFHAFNHAVFKSLLFLCAGSVVHATG
HHCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
TRKIEAFGGLVKSMPVTSALFLIGSVSIAAMPPTNGFAGELLLYQAFFQSFAVTDPLLNI
CCHHHHHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FLIIALSSFALTGALAAALFVKLFGITCLAIPRSEKSRLAEEVQKPMLVGPAVPAAFCIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCEECCCHHHHHHHH
GGLFSKQLLSLAGWKIDFPDLLLLGILLGLIYTALFVIIRSKETGPARISETWGCGIPAL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
APYMEYSSAGFSEPLVMIFKSIYRTKIVNRPEYFDEKESLFKNGKVEIRLLRFFEEYLYI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCC
PPARAIDIFSKYVSKLQNGKLDSYVLYAFLAVIVLIIATGGFV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLETLMYGAISALVFGTVLPLLYRSKNIRKASSGLSLLSSILLLGFAGIILYTRTEPVFP
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
VTGFLPGAGFTLSADRLAAGFILLIGAVVPGVSIYSVEYVEHAKSEARKSLQASLTNLFI
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LAMLMVILAGNMVSFLVFWEIMSLSSLLLVLHDYSSEENKKAGFFYFVMTNISTAFLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHH
FISLFRLTGSADFGPLEYPAADLALPFIFLFIGFGVKAGLVPFHKWLPYAHPAAPSSVSA
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHCCCCHHHHHCCCCCCCCCHHHHH
LMSGVMLKVAVYGFLRFLLSVSAPELWWGVLILTAGSISALFGVIYALKENDIKRMLAYS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
SIENIGIIFTGIGLYAVFKVEGLESLALLSLVGACFHAFNHAVFKSLLFLCAGSVVHATG
HHCCCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
TRKIEAFGGLVKSMPVTSALFLIGSVSIAAMPPTNGFAGELLLYQAFFQSFAVTDPLLNI
CCHHHHHHHHHHHCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FLIIALSSFALTGALAAALFVKLFGITCLAIPRSEKSRLAEEVQKPMLVGPAVPAAFCIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCEECCCHHHHHHHH
GGLFSKQLLSLAGWKIDFPDLLLLGILLGLIYTALFVIIRSKETGPARISETWGCGIPAL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
APYMEYSSAGFSEPLVMIFKSIYRTKIVNRPEYFDEKESLFKNGKVEIRLLRFFEEYLYI
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCC
PPARAIDIFSKYVSKLQNGKLDSYVLYAFLAVIVLIIATGGFV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]