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The map label for this gene is hycD [H]

Identifier: 21227162

GI number: 21227162

Start: 1244201

End: 1245142

Strand: Direct

Name: hycD [H]

Synonym: MM_1060

Alternate gene names: 21227162

Gene position: 1244201-1245142 (Clockwise)

Preceding gene: 21227161

Following gene: 21227163

Centisome position: 30.37

GC content: 48.41

Gene sequence:

>942_bases
ATGAACAGCTCGTTTCTGCTCTTCGCCCTCCTGAATCCCCTGTTTGCCCTGCTGGCAGCCCCTCTATACATGGCTCTGAT
TCGGAAAGTGAAGGCATATGCCCAGGGAAGAAAGGGGCCGGGGCTCTTTCAGGCCTATTACGACTTAAAAAAGCTCATGA
AAAAAGAAACGGTGTATTCCCCTGTTTCTTCATGGGTCATGCGGTTTACTCCGTACCTTAACCTTTCAACCCTTCTTGTC
TGCTCACTTTTCGTACCTCTGGTATTTGTCCCCGAACCTGCCGGAGGGTTGGGGAACATAATTGTTTTCCTGTACCTTCT
GGCGCTTGAAAGGTTCTTTACCGCCCTTGGAGGGCTTGATGCAGGCAGTGCATTCGGAGGCATGGGCAGTTCAAGGTCAA
TGAGCCTTGCTGCTGTAATCGAACCTACCATGGTAATTTCTTTTGCAGCTCTGGCATTTGTCCTGAAAAGCCTTAACCTG
CACCAGATGTTTACACTTACCGCAGGCAAGGTTCTTCCAATCAGCCCGACCCTTGTCCTTGTCGCAATCTCCCTCTTCAT
TATCCTGATTGTGGAGACCGGCAGAATCCCAGTGGACAACCCTGCAACTCACCTTGAACTTACCATGATTAATGAGGCAA
TGATCCTTGAACAGGCAGGAAAGAACCTTGCCCTGCTCGAGCTTGCACATGCCCTTAAACAGCTCCTGTTGATGGGAATT
TTGATTAACACAATCCTCCCGGTAGGGCTCAGTACTGAGATAGACGCAGGAAAAATTGCAGTTTCAGCCCTTCTCTTCCT
TGTAAAGGCAGGGCTGCTTGCAATAATAATAGGGCTCTTTGAATCATCCCTTGCAAAGATGAGGCTATTCAGGCTTCCCG
CATTTTACGTCATGGCGTTTTTCTTTTCAGCCATTACAATCCTTATCGAGGTGTTTGCATGA

Upstream 100 bases:

>100_bases
CAAAATATGTTTCAAAACTGCAGAACGGAAAACTTGACAGTTATGTCCTTTACGCCTTTCTTGCAGTGATTGTACTAATT
ATAGCAACCGGAGGCTTTGT

Downstream 100 bases:

>100_bases
TTGACCCGACCTTCCTTGAAGGCATAATTAAGATCCTTTTCGTCTTTGTCCTCATCAGTGCAGGGTTGATCATTTCAACC
CGAAACCTGAGTTCGGTATT

Product: formate hydrogenlyase subunit 4

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNSSFLLFALLNPLFALLAAPLYMALIRKVKAYAQGRKGPGLFQAYYDLKKLMKKETVYSPVSSWVMRFTPYLNLSTLLV
CSLFVPLVFVPEPAGGLGNIIVFLYLLALERFFTALGGLDAGSAFGGMGSSRSMSLAAVIEPTMVISFAALAFVLKSLNL
HQMFTLTAGKVLPISPTLVLVAISLFIILIVETGRIPVDNPATHLELTMINEAMILEQAGKNLALLELAHALKQLLLMGI
LINTILPVGLSTEIDAGKIAVSALLFLVKAGLLAIIIGLFESSLAKMRLFRLPAFYVMAFFFSAITILIEVFA

Sequences:

>Translated_313_residues
MNSSFLLFALLNPLFALLAAPLYMALIRKVKAYAQGRKGPGLFQAYYDLKKLMKKETVYSPVSSWVMRFTPYLNLSTLLV
CSLFVPLVFVPEPAGGLGNIIVFLYLLALERFFTALGGLDAGSAFGGMGSSRSMSLAAVIEPTMVISFAALAFVLKSLNL
HQMFTLTAGKVLPISPTLVLVAISLFIILIVETGRIPVDNPATHLELTMINEAMILEQAGKNLALLELAHALKQLLLMGI
LINTILPVGLSTEIDAGKIAVSALLFLVKAGLLAIIIGLFESSLAKMRLFRLPAFYVMAFFFSAITILIEVFA
>Mature_313_residues
MNSSFLLFALLNPLFALLAAPLYMALIRKVKAYAQGRKGPGLFQAYYDLKKLMKKETVYSPVSSWVMRFTPYLNLSTLLV
CSLFVPLVFVPEPAGGLGNIIVFLYLLALERFFTALGGLDAGSAFGGMGSSRSMSLAAVIEPTMVISFAALAFVLKSLNL
HQMFTLTAGKVLPISPTLVLVAISLFIILIVETGRIPVDNPATHLELTMINEAMILEQAGKNLALLELAHALKQLLLMGI
LINTILPVGLSTEIDAGKIAVSALLFLVKAGLLAIIIGLFESSLAKMRLFRLPAFYVMAFFFSAITILIEVFA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=298, Percent_Identity=30.8724832214765, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI87082115, Length=289, Percent_Identity=33.2179930795848, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33924; Mature: 33924

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFLLFALLNPLFALLAAPLYMALIRKVKAYAQGRKGPGLFQAYYDLKKLMKKETVYS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PVSSWVMRFTPYLNLSTLLVCSLFVPLVFVPEPAGGLGNIIVFLYLLALERFFTALGGLD
HHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGSAFGGMGSSRSMSLAAVIEPTMVISFAALAFVLKSLNLHQMFTLTAGKVLPISPTLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCHHHHH
VAISLFIILIVETGRIPVDNPATHLELTMINEAMILEQAGKNLALLELAHALKQLLLMGI
HHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LINTILPVGLSTEIDAGKIAVSALLFLVKAGLLAIIIGLFESSLAKMRLFRLPAFYVMAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFSAITILIEVFA
HHHHHHHHHHHCC
>Mature Secondary Structure
MNSSFLLFALLNPLFALLAAPLYMALIRKVKAYAQGRKGPGLFQAYYDLKKLMKKETVYS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PVSSWVMRFTPYLNLSTLLVCSLFVPLVFVPEPAGGLGNIIVFLYLLALERFFTALGGLD
HHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGSAFGGMGSSRSMSLAAVIEPTMVISFAALAFVLKSLNLHQMFTLTAGKVLPISPTLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCHHHHH
VAISLFIILIVETGRIPVDNPATHLELTMINEAMILEQAGKNLALLELAHALKQLLLMGI
HHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LINTILPVGLSTEIDAGKIAVSALLFLVKAGLLAIIIGLFESSLAKMRLFRLPAFYVMAF
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFSAITILIEVFA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]