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The map label for this gene is speB [C]

Identifier: 21227025

GI number: 21227025

Start: 1089822

End: 1090697

Strand: Direct

Name: speB [C]

Synonym: MM_0923

Alternate gene names: 21227025

Gene position: 1089822-1090697 (Clockwise)

Preceding gene: 21227024

Following gene: 21227031

Centisome position: 26.6

GC content: 47.26

Gene sequence:

>876_bases
ATGTTTTTACCCAATTCCTTTATAGATGCCCTCGCAGACTACGAATCTGCACGCTACGTAATTTTTGGCGTACCCTTTGA
TAATACCTCTTCATACCGTGCAGGCAGCCGCTGGGCTCCGGATGCTATGAGGCAGGTTTCTGCAAATTTTGAGAGTTATA
ACCCGGTTTTCGACATAGACCTTGTAGACCTTCCGATATATGATGCAGGAAACCTGGAAACTTCAGCCTCGGTTGACGAG
ACCCTGAGAGACCTTTACGAAGAGGTAAAGGCTCTGCTGAACAACGGAAAGCTTCCTATTATGCTTGGGGGCGAGCACTC
CCTGACTTATTCTACGGTCAAAGCCTGTGCGGAATTCGCAGGGGATGATTTCGGAGTCCTTATTCTTGATGCCCACTTTG
ACCTCAGGGAGGAGTACAGAGGGTTCAAACACAACCATGCATGTGTATCCCGGAATATTCTGGAAAATATCACGGATAAT
CTCGTTTCTATAGGTATAAGGAGCGGGCCCGAAGAAGAATGGATTTTTGCCAGAGAAAATAAGCTGAAATACTACACGGC
AGATGATGTTGAATCCATAGGCATGGTAGAAGTCCTCAAAGAAGCGATTGAATCGCTTGACTGCAGCCAGCTCTACCTTT
CCCTTGATATGGATGCAATAGATCCCGCGTATGCCCCCGGACTGGGAACACCTGAACCTTTCGGGCTGAGTGCCAGGGAT
GTCAGGACTGCGATAAGGACCCTTGCCCCTTACTCAATGGCTTTCGATGTTGTTGAAATTGCCCCTGAATACGACTCCGG
ACAGACAGCGATGCTCGGAGCAAAACTTATGAGAGAATTTATTGCCTCTCATGCAAAAAGCTGCATCAAAAAATAA

Upstream 100 bases:

>100_bases
AAAAACAGACAAACAAAAACTCAGGTTAATACAGAGCCTGAGTTCTCTTTTTTACTAATTAAACAATTTACATCAGGTAT
AATTTACATCAGGTAAGAGT

Downstream 100 bases:

>100_bases
AAGGATAGAATGAAAACATGAGCTATGAACTGTAAATCTACTGCAGGTAAAACGGTAAAAACAGGAAAATATTAGCTCTC
AGTATCAGCTTTCAGGAAGC

Product: agmatinase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MFLPNSFIDALADYESARYVIFGVPFDNTSSYRAGSRWAPDAMRQVSANFESYNPVFDIDLVDLPIYDAGNLETSASVDE
TLRDLYEEVKALLNNGKLPIMLGGEHSLTYSTVKACAEFAGDDFGVLILDAHFDLREEYRGFKHNHACVSRNILENITDN
LVSIGIRSGPEEEWIFARENKLKYYTADDVESIGMVEVLKEAIESLDCSQLYLSLDMDAIDPAYAPGLGTPEPFGLSARD
VRTAIRTLAPYSMAFDVVEIAPEYDSGQTAMLGAKLMREFIASHAKSCIKK

Sequences:

>Translated_291_residues
MFLPNSFIDALADYESARYVIFGVPFDNTSSYRAGSRWAPDAMRQVSANFESYNPVFDIDLVDLPIYDAGNLETSASVDE
TLRDLYEEVKALLNNGKLPIMLGGEHSLTYSTVKACAEFAGDDFGVLILDAHFDLREEYRGFKHNHACVSRNILENITDN
LVSIGIRSGPEEEWIFARENKLKYYTADDVESIGMVEVLKEAIESLDCSQLYLSLDMDAIDPAYAPGLGTPEPFGLSARD
VRTAIRTLAPYSMAFDVVEIAPEYDSGQTAMLGAKLMREFIASHAKSCIKK
>Mature_291_residues
MFLPNSFIDALADYESARYVIFGVPFDNTSSYRAGSRWAPDAMRQVSANFESYNPVFDIDLVDLPIYDAGNLETSASVDE
TLRDLYEEVKALLNNGKLPIMLGGEHSLTYSTVKACAEFAGDDFGVLILDAHFDLREEYRGFKHNHACVSRNILENITDN
LVSIGIRSGPEEEWIFARENKLKYYTADDVESIGMVEVLKEAIESLDCSQLYLSLDMDAIDPAYAPGLGTPEPFGLSARD
VRTAIRTLAPYSMAFDVVEIAPEYDSGQTAMLGAKLMREFIASHAKSCIKK

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=276, Percent_Identity=31.8840579710145, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI10947139, Length=257, Percent_Identity=26.4591439688716, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4502215, Length=307, Percent_Identity=25.4071661237785, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789306, Length=264, Percent_Identity=31.0606060606061, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71996843, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24638886, Length=290, Percent_Identity=25.5172413793103, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32336; Mature: 32336

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLPNSFIDALADYESARYVIFGVPFDNTSSYRAGSRWAPDAMRQVSANFESYNPVFDID
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEE
LVDLPIYDAGNLETSASVDETLRDLYEEVKALLNNGKLPIMLGGEHSLTYSTVKACAEFA
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHC
GDDFGVLILDAHFDLREEYRGFKHNHACVSRNILENITDNLVSIGIRSGPEEEWIFAREN
CCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
KLKYYTADDVESIGMVEVLKEAIESLDCSQLYLSLDMDAIDPAYAPGLGTPEPFGLSARD
CEEEEECCCHHHCCHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHH
VRTAIRTLAPYSMAFDVVEIAPEYDSGQTAMLGAKLMREFIASHAKSCIKK
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLPNSFIDALADYESARYVIFGVPFDNTSSYRAGSRWAPDAMRQVSANFESYNPVFDID
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEE
LVDLPIYDAGNLETSASVDETLRDLYEEVKALLNNGKLPIMLGGEHSLTYSTVKACAEFA
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHC
GDDFGVLILDAHFDLREEYRGFKHNHACVSRNILENITDNLVSIGIRSGPEEEWIFAREN
CCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
KLKYYTADDVESIGMVEVLKEAIESLDCSQLYLSLDMDAIDPAYAPGLGTPEPFGLSARD
CEEEEECCCHHHCCHHHHHHHHHHHCCHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHH
VRTAIRTLAPYSMAFDVVEIAPEYDSGQTAMLGAKLMREFIASHAKSCIKK
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]