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The map label for this gene is yqhM [H]

Identifier: 212224683

GI number: 212224683

Start: 1405939

End: 1406688

Strand: Reverse

Name: yqhM [H]

Synonym: TON_1532

Alternate gene names: 212224683

Gene position: 1406688-1405939 (Counterclockwise)

Preceding gene: 212224684

Following gene: 212224680

Centisome position: 76.14

GC content: 57.07

Gene sequence:

>750_bases
ATGAGGTTCATTCCGCTCATAGTTGCCCGACCCGAGGTTCAGATGGCGATAGACGAGGCAATAATGCGCGCTAGAATCGA
GGGCAAGGTCCCCGACACGGTGAGGCTCTACGCTTTCAGTCCCAGCTCGGTAACTATAGGCCGCTTCCAGAGCGTTAGGC
ACGATGTTGACCTCGATGTTGCCGGGAAGCTCGGAATCCCTGTTGTAAGGCGCATAACCGGCGGCGGAAGCGTCTTCCAC
GACGAGTTCGGTGAGATAACCTATTCTGTGGTCGTCGGCGAGGACTACCATCCCGCCCTGAGGAACGTCGAGGAGAGCTA
CCGCTACCTGGCTGGTCCGCTCGTCGATGCTCTGAAGGAGCTTGGCCTCGACGCCGGCTTCTCCGGCCTTAACGACATCG
TTGCCAACGGCAAGAAGATAAGCGGCTCTGCCCAGACGAGGAGAAAGGGGGTAATCCTCCAGCACGGCACGTTCATGTAC
GCGACGAGGGTGGATGTACTCGCTAAGGTTCTGAGGGTCTCGAAGGCAAAGCTTTCCGATAAAGGAGTTTCGAGCATCTG
GGAGAGGGTTACGACGCTGGAGCGCGAGGGAATAAAGCTGAACCGCTGGGAGACCTACGAGCTGCTGAGAGACAAGTTCT
TTGCTGCGTTTGAGCTGGAAGAAGGCCAGCTGACGGACTACGAGCTCGAGCTTGCAGAGAAACTGATAGAGGAGAGGTAC
GGAAACCCGGAGTGGAACGAGATGAGGTAA

Upstream 100 bases:

>100_bases
TCTGCTCAGTGGTGACCGCTTTCATCACCCGTCAGGAGGGCTTGGGCGTCATCATCGCTGGGCTTAAAAACGCTCTTGAT
TTTTTAACCTTGGTGATAGC

Downstream 100 bases:

>100_bases
GAGGCTATTTTCCCAGAGATTCTATCCCCCTTCTCAGCCTTTTCACCTTCCGATTCATCGTCCAGAGTGTAATGATTTGG
AGTAGTCTATCATGTACTTG

Product: lipoate-protein ligase A, N-terminal section

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVRHDVDLDVAGKLGIPVVRRITGGGSVFH
DEFGEITYSVVVGEDYHPALRNVEESYRYLAGPLVDALKELGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMY
ATRVDVLAKVLRVSKAKLSDKGVSSIWERVTTLEREGIKLNRWETYELLRDKFFAAFELEEGQLTDYELELAEKLIEERY
GNPEWNEMR

Sequences:

>Translated_249_residues
MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVRHDVDLDVAGKLGIPVVRRITGGGSVFH
DEFGEITYSVVVGEDYHPALRNVEESYRYLAGPLVDALKELGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMY
ATRVDVLAKVLRVSKAKLSDKGVSSIWERVTTLEREGIKLNRWETYELLRDKFFAAFELEEGQLTDYELELAEKLIEERY
GNPEWNEMR
>Mature_249_residues
MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVRHDVDLDVAGKLGIPVVRRITGGGSVFH
DEFGEITYSVVVGEDYHPALRNVEESYRYLAGPLVDALKELGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMY
ATRVDVLAKVLRVSKAKLSDKGVSSIWERVTTLEREGIKLNRWETYELLRDKFFAAFELEEGQLTDYELELAEKLIEERY
GNPEWNEMR

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790846, Length=213, Percent_Identity=28.6384976525822, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 28081; Mature: 28081

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVRHDVDLDV
CCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHCCCCCC
AGKLGIPVVRRITGGGSVFHDEFGEITYSVVVGEDYHPALRNVEESYRYLAGPLVDALKE
CCCCCCHHHEEECCCCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMYATRVDVLAKVLRVSKAKLSD
CCCCCCCCHHHHHHHCCCCCCCCHHHHHCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCC
KGVSSIWERVTTLEREGIKLNRWETYELLRDKFFAAFELEEGQLTDYELELAEKLIEERY
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHC
GNPEWNEMR
CCCCCCCCC
>Mature Secondary Structure
MRFIPLIVARPEVQMAIDEAIMRARIEGKVPDTVRLYAFSPSSVTIGRFQSVRHDVDLDV
CCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHHHCCCCCC
AGKLGIPVVRRITGGGSVFHDEFGEITYSVVVGEDYHPALRNVEESYRYLAGPLVDALKE
CCCCCCHHHEEECCCCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGLDAGFSGLNDIVANGKKISGSAQTRRKGVILQHGTFMYATRVDVLAKVLRVSKAKLSD
CCCCCCCCHHHHHHHCCCCCCCCHHHHHCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCC
KGVSSIWERVTTLEREGIKLNRWETYELLRDKFFAAFELEEGQLTDYELELAEKLIEERY
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHC
GNPEWNEMR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]