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The map label for this gene is purB [H]

Identifier: 212224078

GI number: 212224078

Start: 855641

End: 856996

Strand: Reverse

Name: purB [H]

Synonym: TON_0929

Alternate gene names: 212224078

Gene position: 856996-855641 (Counterclockwise)

Preceding gene: 212224082

Following gene: 212224076

Centisome position: 46.38

GC content: 51.99

Gene sequence:

>1356_bases
ATGGCCGTTCATCCGATTGATTATCGCTACGGTAGCGAGGAGATGAGGAGAATTTGGGATGAGGAGAACAAGCTTCAGAA
GCTACTCGATGTTGAGGCTGCCTTGGCTAGGGCTCACGCCAAGCTCGGCAACATCCCCGAAGAGAGCGCCCGCGTTATTT
CCGAGAGAGCTAACACCAAGTGGGTTAAGCTCGAACGCGTTAAGGAGATAGAGAACGAGATACACCATGACATAATGGCC
GTCGTTAAGGCCCTGAGTGAGGTCTGCGGCGAACATGGCAAGTACGTTCATCTAGGTGCGACCTCAAACGACATCATCGA
CACGGCTAACGCTCTTCTCATCAAGGAGTCCCTTGCGATAATCGAGAAGGATCTTAGGGAGCTCCGCTCAATTCTCAAGA
AGCTCGCGGAGGAGCACAAGTACACGGTCTGTGTCGGGAGAACCCACGGGCAACATGCTGTTCCTACAACATACGGCATG
AAGTTTGCCCTCTGGCTCGATGAGATACAGAGACACATAGACAGGATAGAGGAGCTGAAGGAAAGAGTTTTGGTTGGCCA
GATGAGCGGTGCAGTTGGAACGATGGCGTCTTTCGGCGATAAGGGCCTCGAAATTCAGCGTCTCGTCATGGAGGATCTCG
GTCTCAGGCCTGCCAGAATAAGCAGCCAGGTAATCCAGCGCGACGTTTACGCGGAACTAATGATGGTTCTCGCGCTTATA
GCTTCCACTCTCGACAAGATAGCCCTCGAAATCAGAAACCTCCAGAGAACTGAAATCCTTGAAGTGAGCGAGCCCTTCGG
AAAGAAGCAGGTGGGCTCCTCGACAATGCCCCACAAGAGGAACCCCATACGGAGCGAGAAGGTGAGTGGCTTGGCGAGGG
TTCTCTACTCCAATGTCATTCCTGCACTGCTCAACAACCCCCTATGGCACGAGAGGGATCTGACGAACTCCTCTGTCGAG
CGCGTTATTCTTCCTGAGAGCTTTGTCCTACTTGACGAGATGCTCAAGAGCATGAAGAAGGTTCTCTCGGGGCTTGAATT
CTTCCCGGAGAACATTAAGAGGAACCTCTACATGACCCACAACCTCATAATGGCCGAGCCGCTTATGCTCGAGCTTACTG
AAAGGGGCATGGGAAGGCAGGAGGCGCATGAATTGGTGAGAGGACTGGCAATGAAGGCGTTCTACGAGAAGAGGGACCTC
ATGGAGATCGCGAAGGAGAGTGAGGAAGTCAGAAAGTTCCTCAGTGAGGAAGACTTTGAGAGCCTGAAGCCGGAGAACTA
TATTGGCATCGCGCCTCAGATAGTTGATAACGTAATTGCTTATATAGGGGAGAAAGAGCGAAAAGAAGGGATGTAA

Upstream 100 bases:

>100_bases
GGCTTTAATGAATTTATCGAATATCCATCTCTGCTCCTGCATGTTTCTGTTAAAGTAAAATGTTTTAAGCCGTAAGGATT
GCCATAGGCCTGGTGATAGG

Downstream 100 bases:

>100_bases
GGCTTTAATTTATTGTCTCTTATTCTCTACATCGTTTTTGTTGTTTTGTCCACTTCGTCGAATTTATCTCTCAAATTTTT
GGGCGAATTACTACGTTTAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTKWVKLERVKEIENEIHHDIMA
VVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLAIIEKDLRELRSILKKLAEEHKYTVCVGRTHGQHAVPTTYGM
KFALWLDEIQRHIDRIEELKERVLVGQMSGAVGTMASFGDKGLEIQRLVMEDLGLRPARISSQVIQRDVYAELMMVLALI
ASTLDKIALEIRNLQRTEILEVSEPFGKKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVIPALLNNPLWHERDLTNSSVE
RVILPESFVLLDEMLKSMKKVLSGLEFFPENIKRNLYMTHNLIMAEPLMLELTERGMGRQEAHELVRGLAMKAFYEKRDL
MEIAKESEEVRKFLSEEDFESLKPENYIGIAPQIVDNVIAYIGEKERKEGM

Sequences:

>Translated_451_residues
MAVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTKWVKLERVKEIENEIHHDIMA
VVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLAIIEKDLRELRSILKKLAEEHKYTVCVGRTHGQHAVPTTYGM
KFALWLDEIQRHIDRIEELKERVLVGQMSGAVGTMASFGDKGLEIQRLVMEDLGLRPARISSQVIQRDVYAELMMVLALI
ASTLDKIALEIRNLQRTEILEVSEPFGKKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVIPALLNNPLWHERDLTNSSVE
RVILPESFVLLDEMLKSMKKVLSGLEFFPENIKRNLYMTHNLIMAEPLMLELTERGMGRQEAHELVRGLAMKAFYEKRDL
MEIAKESEEVRKFLSEEDFESLKPENYIGIAPQIVDNVIAYIGEKERKEGM
>Mature_450_residues
AVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTKWVKLERVKEIENEIHHDIMAV
VKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLAIIEKDLRELRSILKKLAEEHKYTVCVGRTHGQHAVPTTYGMK
FALWLDEIQRHIDRIEELKERVLVGQMSGAVGTMASFGDKGLEIQRLVMEDLGLRPARISSQVIQRDVYAELMMVLALIA
STLDKIALEIRNLQRTEILEVSEPFGKKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVIPALLNNPLWHERDLTNSSVER
VILPESFVLLDEMLKSMKKVLSGLEFFPENIKRNLYMTHNLIMAEPLMLELTERGMGRQEAHELVRGLAMKAFYEKRDLM
EIAKESEEVRKFLSEEDFESLKPENYIGIAPQIVDNVIAYIGEKERKEGM

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=459, Percent_Identity=28.7581699346405, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=29.8701298701299, Blast_Score=179, Evalue=5e-45,
Organism=Escherichia coli, GI1787376, Length=465, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790398, Length=399, Percent_Identity=24.5614035087719, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=424, Percent_Identity=28.0660377358491, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32564234, Length=337, Percent_Identity=28.486646884273, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553882, Length=351, Percent_Identity=27.0655270655271, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565146, Length=345, Percent_Identity=27.2463768115942, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=452, Percent_Identity=30.3097345132743, Blast_Score=189, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6324993, Length=347, Percent_Identity=24.7838616714697, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=29.7029702970297, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24640179, Length=297, Percent_Identity=27.6094276094276, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24640177, Length=297, Percent_Identity=27.6094276094276, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI78710009, Length=295, Percent_Identity=27.7966101694915, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24662684, Length=221, Percent_Identity=28.9592760180996, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221473854, Length=319, Percent_Identity=25.0783699059561, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI78706858, Length=319, Percent_Identity=25.0783699059561, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51497; Mature: 51365

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTK
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
WVKLERVKEIENEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
IEKDLRELRSILKKLAEEHKYTVCVGRTHGQHAVPTTYGMKFALWLDEIQRHIDRIEELK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVLVGQMSGAVGTMASFGDKGLEIQRLVMEDLGLRPARISSQVIQRDVYAELMMVLALI
HHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASTLDKIALEIRNLQRTEILEVSEPFGKKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVI
HHHHHHHHHHHHHCHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PALLNNPLWHERDLTNSSVERVILPESFVLLDEMLKSMKKVLSGLEFFPENIKRNLYMTH
HHHHCCCCCCCCCCCCHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NLIMAEPLMLELTERGMGRQEAHELVRGLAMKAFYEKRDLMEIAKESEEVRKFLSEEDFE
HHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SLKPENYIGIAPQIVDNVIAYIGEKERKEGM
HCCCCCCCCCCHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure 
AVHPIDYRYGSEEMRRIWDEENKLQKLLDVEAALARAHAKLGNIPEESARVISERANTK
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
WVKLERVKEIENEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
IEKDLRELRSILKKLAEEHKYTVCVGRTHGQHAVPTTYGMKFALWLDEIQRHIDRIEELK
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVLVGQMSGAVGTMASFGDKGLEIQRLVMEDLGLRPARISSQVIQRDVYAELMMVLALI
HHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASTLDKIALEIRNLQRTEILEVSEPFGKKQVGSSTMPHKRNPIRSEKVSGLARVLYSNVI
HHHHHHHHHHHHHCHHHHHHHCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PALLNNPLWHERDLTNSSVERVILPESFVLLDEMLKSMKKVLSGLEFFPENIKRNLYMTH
HHHHCCCCCCCCCCCCHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NLIMAEPLMLELTERGMGRQEAHELVRGLAMKAFYEKRDLMEIAKESEEVRKFLSEEDFE
HHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SLKPENYIGIAPQIVDNVIAYIGEKERKEGM
HCCCCCCCCCCHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]