Click here to switch to the map view.

The map label for this gene is fpoH [H]

Identifier: 212223637

GI number: 212223637

Start: 449887

End: 450816

Strand: Reverse

Name: fpoH [H]

Synonym: TON_0490

Alternate gene names: 212223637

Gene position: 450816-449887 (Counterclockwise)

Preceding gene: 212223638

Following gene: 212223636

Centisome position: 24.4

GC content: 52.8

Gene sequence:

>930_bases
ATGCTTGAGGTAGCGCTGAAGGCTTTATTCCTCCTAATTTATGCGACCTTCGTCGGGTTCATGTTCATGGGAATAATCAG
GAAAGTGAGCGCAAGGATACACAGAAGGGTAGGACCGCCAATCTATCAGCCCATACTTGACACCATCAAGTTCTTCGGCA
AGAAGGAGAACATTACCCACGGATTAATCTACGACTTCGGCATCATCTACGCCCTTGGAGCTACGGTACTGGCTTTGTTC
TTCATCCCGCTGGGAAGCGTCAGCATACTCAGGGCCTACGGGGACCTCATCCTCATCACCTTCCTCCTCGAGATACCGAT
GCTGGGAATAATGTTCGCTGCAATGAGCTCAGGAAACCCCTATGCAGGAATAGGCGCCCAGAGGGCCCTGCTTACTATGC
TGGCCATTCAGGTGCCCCTCGGCTTCGCGATAATAGCACTCGCAGAGTTTTACGGAACCTTCAGCACCTACGAGATAGTC
ATGGCTCAGCAGATCAGCGGATGGAGTATAATACACCTGCCGCTCCTCTTAGCCGCCATAGCCTACGACATCGTCCTTCA
GGCAATGTTCGGCAAGGAGCCCTTCGACATCATGATTGCACCAGGTGAAATCTCCCTCGGTCCGATGGTCGAGTTCGGCG
GAAAACACATGGGGATACTCCAGATACAGCACGCCATAGCCCTCTTCGCGGAGACGCTGTTCTTCAGCAACATATTCCTA
GGCGGAGCAGTTATTACTGCCTTTGCAAGCCCGATACTGAACACCATAGCCAGCCTTGCAATACTCCTCGTCAAGCAGAT
AGCGGTGCTCCTCATAGCGGTATTCGTCAGCACGATATTCCCGAGGTTCACGATTGACCAGGCGGCCAAGTTCTACTGGA
AGTGGCCGACCATCATAGCCGCACTCGGAGCGATACTTGCAAGCCTGTGA

Upstream 100 bases:

>100_bases
CGAGACCATGAGGAACTACGTCTACAATGGAAACGTCCAGAGCTATGCCTGGTATCTTGCCATAGTCCTGTTAATCCTCG
CTATGTGGGGGGTGTGAAGA

Downstream 100 bases:

>100_bases
GGTGATGAAAATGGTGGACTGGAGACTCTTTGAACCTCTCTTTAATTATGCGAGAAAGAAGAGCCTTTGGATTGTGTCGT
TCTGTACCGGATGCGGCGGT

Product: NADH dehydrogenase subunit

Products: NAD+; ubiquinol

Alternate protein names: F(420)H(2) dehydrogenase subunit H; FPO subunit H [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLEVALKALFLLIYATFVGFMFMGIIRKVSARIHRRVGPPIYQPILDTIKFFGKKENITHGLIYDFGIIYALGATVLALF
FIPLGSVSILRAYGDLILITFLLEIPMLGIMFAAMSSGNPYAGIGAQRALLTMLAIQVPLGFAIIALAEFYGTFSTYEIV
MAQQISGWSIIHLPLLLAAIAYDIVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGILQIQHAIALFAETLFFSNIFL
GGAVITAFASPILNTIASLAILLVKQIAVLLIAVFVSTIFPRFTIDQAAKFYWKWPTIIAALGAILASL

Sequences:

>Translated_309_residues
MLEVALKALFLLIYATFVGFMFMGIIRKVSARIHRRVGPPIYQPILDTIKFFGKKENITHGLIYDFGIIYALGATVLALF
FIPLGSVSILRAYGDLILITFLLEIPMLGIMFAAMSSGNPYAGIGAQRALLTMLAIQVPLGFAIIALAEFYGTFSTYEIV
MAQQISGWSIIHLPLLLAAIAYDIVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGILQIQHAIALFAETLFFSNIFL
GGAVITAFASPILNTIASLAILLVKQIAVLLIAVFVSTIFPRFTIDQAAKFYWKWPTIIAALGAILASL
>Mature_309_residues
MLEVALKALFLLIYATFVGFMFMGIIRKVSARIHRRVGPPIYQPILDTIKFFGKKENITHGLIYDFGIIYALGATVLALF
FIPLGSVSILRAYGDLILITFLLEIPMLGIMFAAMSSGNPYAGIGAQRALLTMLAIQVPLGFAIIALAEFYGTFSTYEIV
MAQQISGWSIIHLPLLLAAIAYDIVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGILQIQHAIALFAETLFFSNIFL
GGAVITAFASPILNTIASLAILLVKQIAVLLIAVFVSTIFPRFTIDQAAKFYWKWPTIIAALGAILASL

Specific function: FPO shuttles electrons from F(420)H(2), via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H(2):heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=275, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082115, Length=198, Percent_Identity=29.2929292929293, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788618, Length=300, Percent_Identity=25.3333333333333, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023021
- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.6.5.3

Molecular weight: Translated: 33672; Mature: 33672

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVALKALFLLIYATFVGFMFMGIIRKVSARIHRRVGPPIYQPILDTIKFFGKKENITH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
GLIYDFGIIYALGATVLALFFIPLGSVSILRAYGDLILITFLLEIPMLGIMFAAMSSGNP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YAGIGAQRALLTMLAIQVPLGFAIIALAEFYGTFSTYEIVMAQQISGWSIIHLPLLLAAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AYDIVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGILQIQHAIALFAETLFFSNIFL
HHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGAVITAFASPILNTIASLAILLVKQIAVLLIAVFVSTIFPRFTIDQAAKFYWKWPTIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALGAILASL
HHHHHHHCC
>Mature Secondary Structure
MLEVALKALFLLIYATFVGFMFMGIIRKVSARIHRRVGPPIYQPILDTIKFFGKKENITH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
GLIYDFGIIYALGATVLALFFIPLGSVSILRAYGDLILITFLLEIPMLGIMFAAMSSGNP
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YAGIGAQRALLTMLAIQVPLGFAIIALAEFYGTFSTYEIVMAQQISGWSIIHLPLLLAAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AYDIVLQAMFGKEPFDIMIAPGEISLGPMVEFGGKHMGILQIQHAIALFAETLFFSNIFL
HHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GGAVITAFASPILNTIASLAILLVKQIAVLLIAVFVSTIFPRFTIDQAAKFYWKWPTIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALGAILASL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10751389; 12125824 [H]