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The map label for this gene is nuoB [H]

Identifier: 212223636

GI number: 212223636

Start: 449304

End: 449876

Strand: Reverse

Name: nuoB [H]

Synonym: TON_0489

Alternate gene names: 212223636

Gene position: 449876-449304 (Counterclockwise)

Preceding gene: 212223637

Following gene: 212223635

Centisome position: 24.35

GC content: 52.88

Gene sequence:

>573_bases
ATGGTGGACTGGAGACTCTTTGAACCTCTCTTTAATTATGCGAGAAAGAAGAGCCTTTGGATTGTGTCGTTCTGTACCGG
ATGCGGCGGTATAGAGATGCCGCCCCTCATGACGTCAAGGTATGACCTAGAGCGATTCGGTATGATACCGGACCCGAGTC
CAAGACAGTACGACCTCTTCCTCATCACGGGCTACGTTACGCCAAAGACCCTCAAAAGAATCATAATCACCTACGAAATG
GCACCTGATCCAAAGTACGTCCTGGCGCACGGCTCCTGCCCGCTCAACGGTGGAATCTACTGGGACGCCTACAACGCTAT
CAAGCACCTCGACAAGTACATCCCGGTCGATGTCGTCATAGCCGGATGCATGCCGCGGCCAGAGGCAGTCATGGACGGAA
TCCAGAAGATAATGGAGATGATTGAGAACGGAACAGCAGACGGATGGAAGAGGTACAAGGAGAACTACGAGTGGTACAAG
AAGAACCAGGACGAGCTCTTCGGCGAAGGATGGCGTGAGAGGGAAGCCAGAAGGTGGATCCCATGGCTGGTGGACAAGAA
AAAGGAGGAGTGA

Upstream 100 bases:

>100_bases
CCGAGGTTCACGATTGACCAGGCGGCCAAGTTCTACTGGAAGTGGCCGACCATCATAGCCGCACTCGGAGCGATACTTGC
AAGCCTGTGAGGTGATGAAA

Downstream 100 bases:

>100_bases
ACGAGATGGGGGAAGTTAAGTGGGAGAGAGAGCAGATGCTCGTTGACAAAATCCTTGAAAAAGCCCCCTACGCCGAGGGC
AAGGTGCGGCGCGAACGGAG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MVDWRLFEPLFNYARKKSLWIVSFCTGCGGIEMPPLMTSRYDLERFGMIPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPLNGGIYWDAYNAIKHLDKYIPVDVVIAGCMPRPEAVMDGIQKIMEMIENGTADGWKRYKENYEWYK
KNQDELFGEGWREREARRWIPWLVDKKKEE

Sequences:

>Translated_190_residues
MVDWRLFEPLFNYARKKSLWIVSFCTGCGGIEMPPLMTSRYDLERFGMIPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPLNGGIYWDAYNAIKHLDKYIPVDVVIAGCMPRPEAVMDGIQKIMEMIENGTADGWKRYKENYEWYK
KNQDELFGEGWREREARRWIPWLVDKKKEE
>Mature_190_residues
MVDWRLFEPLFNYARKKSLWIVSFCTGCGGIEMPPLMTSRYDLERFGMIPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPLNGGIYWDAYNAIKHLDKYIPVDVVIAGCMPRPEAVMDGIQKIMEMIENGTADGWKRYKENYEWYK
KNQDELFGEGWREREARRWIPWLVDKKKEE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=155, Percent_Identity=34.8387096774194, Blast_Score=111, Evalue=3e-25,
Organism=Escherichia coli, GI1788624, Length=133, Percent_Identity=39.0977443609023, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789074, Length=131, Percent_Identity=37.4045801526718, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=33.5483870967742, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859983, Length=163, Percent_Identity=33.1288343558282, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24642371, Length=163, Percent_Identity=33.1288343558282, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24651058, Length=155, Percent_Identity=33.5483870967742, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 22402; Mature: 22402

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDWRLFEPLFNYARKKSLWIVSFCTGCGGIEMPPLMTSRYDLERFGMIPDPSPRQYDLF
CCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
LITGYVTPKTLKRIIITYEMAPDPKYVLAHGSCPLNGGIYWDAYNAIKHLDKYIPVDVVI
EEEECCCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCHHHHE
AGCMPRPEAVMDGIQKIMEMIENGTADGWKRYKENYEWYKKNQDELFGEGWREREARRWI
ECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCHHHHCCCHHHHHHHHCC
PWLVDKKKEE
CHHHCCCCCC
>Mature Secondary Structure
MVDWRLFEPLFNYARKKSLWIVSFCTGCGGIEMPPLMTSRYDLERFGMIPDPSPRQYDLF
CCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEE
LITGYVTPKTLKRIIITYEMAPDPKYVLAHGSCPLNGGIYWDAYNAIKHLDKYIPVDVVI
EEEECCCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCHHHHE
AGCMPRPEAVMDGIQKIMEMIENGTADGWKRYKENYEWYKKNQDELFGEGWREREARRWI
ECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHCCCHHHHCCCHHHHHHHHCC
PWLVDKKKEE
CHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA