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The map label for this gene is mdtL [H]

Identifier: 209696386

GI number: 209696386

Start: 3242186

End: 3243391

Strand: Direct

Name: mdtL [H]

Synonym: VSAL_I2992

Alternate gene names: 209696386

Gene position: 3242186-3243391 (Clockwise)

Preceding gene: 209696382

Following gene: 209696387

Centisome position: 97.5

GC content: 43.62

Gene sequence:

>1206_bases
ATGCACACGCCAAGCTCAAGACCATCAGATAAAAAATTGATCACCCTTATGGTGATGCTCGTTTTATTCAGTCCGTTAGC
GATTGATATTTATCTGCCTGCATTACCGATGATGGTAAGCGAATTCCAAGTTGCATCAACATTAGGACAAGACACGATAA
CGTGGTTCATGTTCAGTATGGGATTAGGCCAATTGTTTGCTGGTCCATTAGCGGATCGTTATGGCCGTCGTCCTATCGCT
CTTACTGGTATTGTTATTTATGGTTTGAGTGCGGCATTAGCGTATTCAGCTCAAGAAATGGAAATGCTGTTGTTGGCTCG
TTTACTTCAAGGTTTTGGTGCCTGTGCGACTTCTGTTGCGGCGTTCGCATCAGTACGAGACTCATTTGGTGCAGAGAAGA
GTGGTCGAATGATCAGTTATTTGAATGGCGCGATTTGTTTTATTCCTGCGTTAGCACCAATTTTAGGCAGTTATTTAACG
ATGCATTTTGGTTGGCGTTCAAACTTCAGTTTCATGGCAGGGTTTGCATTAGTTAGTTTTGTGATTATCGCGTTGAACTA
TAAAGAAACAAAACCATCAAATACCTTACAAACAGGGCGATTGATCAGTCCATCTCGTTATTGGTCAGTGCTAAAGCATC
CCGCCTTTTTGTTCCATGCGACATTATGTATGTTAGCGATGGGCGTCATTTTAGCGTATGTGACATCGGCACCGGTATTA
CTGATGGATAAACTTGGGCTTACAATGCAAGAGTTTACCAATTGGTTTGCGGTGAATGCGGTACTAAATATCATGGCATG
TTTTGCTGCACCAAAATTCATGATCAAATTCGGAACTCGTAATGCATTACTTTTCGGCCTCAGTATTTTAGTGGTTGCCG
GTGGGTTAATGTTAGGGTTCCAACATATTCAGCAAGCTTGGGCGTTTATGATCCCGATTTTCTTCTCGTCAGTAGGCTTC
GCATGGGTACTGGGTTCGGCGGCAGGTAAGGCACTAGCACCATTCGGTGATAGAGCAGGAACTGCGGCAGCATTATTAGG
GCTGTTTCAAATGAGTGGTGCGGGAATCATTGTTGGCACAATGCAAAGATTAGCACTCCCTTCAGCACAACTCTTAGCAC
TAATGATGTGGCTTCTCATCCCAGGCTTGCTGATCCTATTAAGTCGCGTTGGTAAACGATGGCATTCAGATCCAATTACT
GAATAA

Upstream 100 bases:

>100_bases
ATATTCCAAATTAGAATGTTAGATCTGAATATAAACCATTTCTGTTCATATCTCTAATTGAGTACACTCCCGATTCAAAC
ATTAGGAGTTAACACGAAAC

Downstream 100 bases:

>100_bases
TTTCAATTGATGAATAAAAAAACAGCAACATTGCTTTTTTAATAGATCGAGATAAGATGGTTTTAGTGTATATTCACCCC
TTATCTCGTTTTAATCGTTA

Product: MFS transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MHTPSSRPSDKKLITLMVMLVLFSPLAIDIYLPALPMMVSEFQVASTLGQDTITWFMFSMGLGQLFAGPLADRYGRRPIA
LTGIVIYGLSAALAYSAQEMEMLLLARLLQGFGACATSVAAFASVRDSFGAEKSGRMISYLNGAICFIPALAPILGSYLT
MHFGWRSNFSFMAGFALVSFVIIALNYKETKPSNTLQTGRLISPSRYWSVLKHPAFLFHATLCMLAMGVILAYVTSAPVL
LMDKLGLTMQEFTNWFAVNAVLNIMACFAAPKFMIKFGTRNALLFGLSILVVAGGLMLGFQHIQQAWAFMIPIFFSSVGF
AWVLGSAAGKALAPFGDRAGTAAALLGLFQMSGAGIIVGTMQRLALPSAQLLALMMWLLIPGLLILLSRVGKRWHSDPIT
E

Sequences:

>Translated_401_residues
MHTPSSRPSDKKLITLMVMLVLFSPLAIDIYLPALPMMVSEFQVASTLGQDTITWFMFSMGLGQLFAGPLADRYGRRPIA
LTGIVIYGLSAALAYSAQEMEMLLLARLLQGFGACATSVAAFASVRDSFGAEKSGRMISYLNGAICFIPALAPILGSYLT
MHFGWRSNFSFMAGFALVSFVIIALNYKETKPSNTLQTGRLISPSRYWSVLKHPAFLFHATLCMLAMGVILAYVTSAPVL
LMDKLGLTMQEFTNWFAVNAVLNIMACFAAPKFMIKFGTRNALLFGLSILVVAGGLMLGFQHIQQAWAFMIPIFFSSVGF
AWVLGSAAGKALAPFGDRAGTAAALLGLFQMSGAGIIVGTMQRLALPSAQLLALMMWLLIPGLLILLSRVGKRWHSDPIT
E
>Mature_401_residues
MHTPSSRPSDKKLITLMVMLVLFSPLAIDIYLPALPMMVSEFQVASTLGQDTITWFMFSMGLGQLFAGPLADRYGRRPIA
LTGIVIYGLSAALAYSAQEMEMLLLARLLQGFGACATSVAAFASVRDSFGAEKSGRMISYLNGAICFIPALAPILGSYLT
MHFGWRSNFSFMAGFALVSFVIIALNYKETKPSNTLQTGRLISPSRYWSVLKHPAFLFHATLCMLAMGVILAYVTSAPVL
LMDKLGLTMQEFTNWFAVNAVLNIMACFAAPKFMIKFGTRNALLFGLSILVVAGGLMLGFQHIQQAWAFMIPIFFSSVGF
AWVLGSAAGKALAPFGDRAGTAAALLGLFQMSGAGIIVGTMQRLALPSAQLLALMMWLLIPGLLILLSRVGKRWHSDPIT
E

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=337, Percent_Identity=30.8605341246291, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788509, Length=371, Percent_Identity=26.9541778975741, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI48994889, Length=189, Percent_Identity=31.2169312169312, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082312, Length=338, Percent_Identity=27.5147928994083, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790794, Length=228, Percent_Identity=27.1929824561404, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787065, Length=232, Percent_Identity=24.5689655172414, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324264, Length=205, Percent_Identity=26.3414634146341, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43445; Mature: 43445

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]