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The map label for this gene is glyS

Identifier: 209696359

GI number: 209696359

Start: 3209940

End: 3212006

Strand: Direct

Name: glyS

Synonym: VSAL_I2965

Alternate gene names: 209696359

Gene position: 3209940-3212006 (Clockwise)

Preceding gene: 209696358

Following gene: 209696360

Centisome position: 96.53

GC content: 44.22

Gene sequence:

>2067_bases
ATGGCGAAGAATTTTCTAATCGAATTAGGTACAGAAGAGCTTCCACCGAAAGCACTTCGTTCATTAGCTGAAGCGTTTGC
TGCAAATTTTGAAGCCGGCCTTAAAGCGGCTGGTTTAGCTCATCAAGGCATTAAGTGGTATGCAACGCCTCGTCGTTTAG
CGCTTAAAATCGCTGAATTAGATGAAGGCCAAGCAGACAAAATCGTTGAAAAGCGTGGTCCTGCAATCGCTTCTGCATTT
GATGCTGACGGTAATCCAACTAAAGCAGCTCAAGGCTGGGCTCGTGGTAACGGTATTACGGTTGAGCAGGCTGAGCGTCT
AAAAACAGACAAAGGCGAATGGCTACTTCATAAAGAAGAAGTAAAAGGCCAACCGGTTAAAGGACTTGTTGTTGAATTAG
CAGCAAAAGCCCTGGCTGGTTTACCAATTCCTAAAGCAATGCGTTGGGGTAACTCAGACATTCAATTTATTCGTCCAGTG
AAAACACTGACAATCCTATTGGGTGACGAACTGATTGAAGGCACTATCTTAGGTGTTGCTTCTACTCGTACTATCCGTGG
CCACCGTTTCATGGGTGAAAGCGAGTTTACGATTGATTCTGCTGACCAATACCCTGCGATCTTAGAAGAACGCGGTAAAG
TAATGGCTGACTACGATGCGCGTAAAGCCATCATCCTTGCTGGTGCTAAAAAAGCAGCAGAAGCGGTTGGCGGTATTGCG
GATCTAGAAGACGAATTAGTTGAAGAAGTTACGTCTCTTGTTGAATGGCCAGTGGTATTGACCGCTAAGTTTGAGCAAGA
GTTCTTGAATGTTCCTTCTGAAGCGTTGGTTTACACAATGAAGGGCGATCAAAAGTACTTCCCTGTGTACGACCAAGAGA
AGAACCTACTTCCTAACTTCATCTTTGTTACTAACATCGAATCTAAAGAACCTCGTCATATTATCGAAGGTAATGAGAAA
GTGGTTCGCCCACGTCTTGCTGATGCTGAATTCTTCTTCAATACAGACCGTAAGCGCCCGCTTATCGATCGTCTGCCAGA
ACTAGAACAAGCAATATTCCAGAAGCAATTGGGCACAATCAAAGACAAAACAGACCGTATTACTGAACTTGCTGGCTACA
TCGCTGAGCAAATTGGTGCGGACGTTGAGAAATCGCAACGTGCAGGTCTTTTAGCTAAGTGTGACTTAATGACCTCAATG
GTATTTGAATTTACAGATACTCAAGGTGTTATGGGTATGCACTACGCAACTCATGATGGTGAAGATGCACAAGTCGCATT
AGCGCTTTACGAGCAATACATGCCTCGTTTCGCGGGTGATGACTTACCAAGTACCGATGTTTCTGCATCAGTAGCAATGG
CAGATAAGCTAGATACGCTAGTGGGTATCTTCGGCATTGGTCAAGCTCCTAAGGGGTCTGATCCATTTGCACTTCGTCGT
GCTGCGTTAGGTATCTTACGTATCATCGTTGAAAAAGGGTACAACTTAGATTTAGTTGACCTTGTTGCTAAAGCACAATC
TTTGTTTGGCGATAAATTAACGAATGCAAACGTAGACACTGATGTGATTGATTTCATGTTAGGCCGTTTCCGTGCATGGT
ATCAAGATGAAGGTTTCAGCGTAGATATCATCCAAGCGGTACTTGCTCGTCGTCCAACCAAGCCTGCTGATTTTGATCAA
CGTGTGAAAGCGGTATCTCACTTCCGTGAATTAGACGCGGCTGAGTCTCTAGCGGCAGCGAATAAGCGCGTAGGTAATAT
CCTGGCGAAATTCGATGGCGAACTGGCTCAAGAAATCGATCTAGCTCTTCTTCAAGAAGACGCTGAGAAAGTATTGGCTG
AGAAAGTTGAAATTCTAGCAGAAGCACTAGAGCCAGTATTTATTGCTGGTAATTATCAAGAAGCATTAAGCCGCCTAGCT
GAGCTACGTGAGCCTGTCGATGCGTTCTTTGATGGTGTAATGGTAATGGCTGACGATGAGGCGCTTAAGCTGAATCGTCT
AACTTTGCTGAATAAGCTACGTAACTTATTCTTAGATATTGCTGATATTTCTCTTCTTCAAAAATAG

Upstream 100 bases:

>100_bases
TAACTAAATCGGTGGCAGAAGCCTATTACGCATCACGTGAAGCGCTAGGCTTCCCAATGTGTCGCTCTACTAAACCAACA
GCGACAGAGGAGAAGTAATC

Downstream 100 bases:

>100_bases
AAATTCATATATCTGATGAAATACCTCGCTATGGCGGGGTATTTTTTTGTCTAAATATCGTGTTTTCGAAAATTAGAGGT
TAATTATATCGATAACAGTG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKNFLIELGTEELPPKALRSLAEAFAANFEAGLKAAGLAHQGIKWYATPRRLALKIAELDEGQADKIVEKRGPAIASAF
DADGNPTKAAQGWARGNGITVEQAERLKTDKGEWLLHKEEVKGQPVKGLVVELAAKALAGLPIPKAMRWGNSDIQFIRPV
KTLTILLGDELIEGTILGVASTRTIRGHRFMGESEFTIDSADQYPAILEERGKVMADYDARKAIILAGAKKAAEAVGGIA
DLEDELVEEVTSLVEWPVVLTAKFEQEFLNVPSEALVYTMKGDQKYFPVYDQEKNLLPNFIFVTNIESKEPRHIIEGNEK
VVRPRLADAEFFFNTDRKRPLIDRLPELEQAIFQKQLGTIKDKTDRITELAGYIAEQIGADVEKSQRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYATHDGEDAQVALALYEQYMPRFAGDDLPSTDVSASVAMADKLDTLVGIFGIGQAPKGSDPFALRR
AALGILRIIVEKGYNLDLVDLVAKAQSLFGDKLTNANVDTDVIDFMLGRFRAWYQDEGFSVDIIQAVLARRPTKPADFDQ
RVKAVSHFRELDAAESLAAANKRVGNILAKFDGELAQEIDLALLQEDAEKVLAEKVEILAEALEPVFIAGNYQEALSRLA
ELREPVDAFFDGVMVMADDEALKLNRLTLLNKLRNLFLDIADISLLQK

Sequences:

>Translated_688_residues
MAKNFLIELGTEELPPKALRSLAEAFAANFEAGLKAAGLAHQGIKWYATPRRLALKIAELDEGQADKIVEKRGPAIASAF
DADGNPTKAAQGWARGNGITVEQAERLKTDKGEWLLHKEEVKGQPVKGLVVELAAKALAGLPIPKAMRWGNSDIQFIRPV
KTLTILLGDELIEGTILGVASTRTIRGHRFMGESEFTIDSADQYPAILEERGKVMADYDARKAIILAGAKKAAEAVGGIA
DLEDELVEEVTSLVEWPVVLTAKFEQEFLNVPSEALVYTMKGDQKYFPVYDQEKNLLPNFIFVTNIESKEPRHIIEGNEK
VVRPRLADAEFFFNTDRKRPLIDRLPELEQAIFQKQLGTIKDKTDRITELAGYIAEQIGADVEKSQRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYATHDGEDAQVALALYEQYMPRFAGDDLPSTDVSASVAMADKLDTLVGIFGIGQAPKGSDPFALRR
AALGILRIIVEKGYNLDLVDLVAKAQSLFGDKLTNANVDTDVIDFMLGRFRAWYQDEGFSVDIIQAVLARRPTKPADFDQ
RVKAVSHFRELDAAESLAAANKRVGNILAKFDGELAQEIDLALLQEDAEKVLAEKVEILAEALEPVFIAGNYQEALSRLA
ELREPVDAFFDGVMVMADDEALKLNRLTLLNKLRNLFLDIADISLLQK
>Mature_687_residues
AKNFLIELGTEELPPKALRSLAEAFAANFEAGLKAAGLAHQGIKWYATPRRLALKIAELDEGQADKIVEKRGPAIASAFD
ADGNPTKAAQGWARGNGITVEQAERLKTDKGEWLLHKEEVKGQPVKGLVVELAAKALAGLPIPKAMRWGNSDIQFIRPVK
TLTILLGDELIEGTILGVASTRTIRGHRFMGESEFTIDSADQYPAILEERGKVMADYDARKAIILAGAKKAAEAVGGIAD
LEDELVEEVTSLVEWPVVLTAKFEQEFLNVPSEALVYTMKGDQKYFPVYDQEKNLLPNFIFVTNIESKEPRHIIEGNEKV
VRPRLADAEFFFNTDRKRPLIDRLPELEQAIFQKQLGTIKDKTDRITELAGYIAEQIGADVEKSQRAGLLAKCDLMTSMV
FEFTDTQGVMGMHYATHDGEDAQVALALYEQYMPRFAGDDLPSTDVSASVAMADKLDTLVGIFGIGQAPKGSDPFALRRA
ALGILRIIVEKGYNLDLVDLVAKAQSLFGDKLTNANVDTDVIDFMLGRFRAWYQDEGFSVDIIQAVLARRPTKPADFDQR
VKAVSHFRELDAAESLAAANKRVGNILAKFDGELAQEIDLALLQEDAEKVLAEKVEILAEALEPVFIAGNYQEALSRLAE
LREPVDAFFDGVMVMADDEALKLNRLTLLNKLRNLFLDIADISLLQK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=686, Percent_Identity=70.5539358600583, Blast_Score=976, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ALISL (B6EGT3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264290.1
- GeneID:   6988610
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2965
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75891; Mature: 75760

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNFLIELGTEELPPKALRSLAEAFAANFEAGLKAAGLAHQGIKWYATPRRLALKIAEL
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHCHHHCCCEEEECHHHHHEEHHHC
DEGQADKIVEKRGPAIASAFDADGNPTKAAQGWARGNGITVEQAERLKTDKGEWLLHKEE
CCCCHHHHHHHCCCCCEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEHHH
VKGQPVKGLVVELAAKALAGLPIPKAMRWGNSDIQFIRPVKTLTILLGDELIEGTILGVA
CCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
STRTIRGHRFMGESEFTIDSADQYPAILEERGKVMADYDARKAIILAGAKKAAEAVGGIA
CCCEECCCCCCCCCCCEECCCCCCHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHCCHH
DLEDELVEEVTSLVEWPVVLTAKFEQEFLNVPSEALVYTMKGDQKYFPVYDQEKNLLPNF
HHHHHHHHHHHHHHHCCHHEEEHHHHHHHCCCCHHEEEEECCCCEECCCCCCCCCCCCCE
IFVTNIESKEPRHIIEGNEKVVRPRLADAEFFFNTDRKRPLIDRLPELEQAIFQKQLGTI
EEEEECCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCCHHHHCHHHHHHHHHHHHCCH
KDKTDRITELAGYIAEQIGADVEKSQRAGLLAKCDLMTSMVFEFTDTQGVMGMHYATHDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EDAQVALALYEQYMPRFAGDDLPSTDVSASVAMADKLDTLVGIFGIGQAPKGSDPFALRR
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AALGILRIIVEKGYNLDLVDLVAKAQSLFGDKLTNANVDTDVIDFMLGRFRAWYQDEGFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VDIIQAVLARRPTKPADFDQRVKAVSHFRELDAAESLAAANKRVGNILAKFDGELAQEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LALLQEDAEKVLAEKVEILAEALEPVFIAGNYQEALSRLAELREPVDAFFDGVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
ALKLNRLTLLNKLRNLFLDIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNFLIELGTEELPPKALRSLAEAFAANFEAGLKAAGLAHQGIKWYATPRRLALKIAEL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHCHHHCCCEEEECHHHHHEEHHHC
DEGQADKIVEKRGPAIASAFDADGNPTKAAQGWARGNGITVEQAERLKTDKGEWLLHKEE
CCCCHHHHHHHCCCCCEECCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEHHH
VKGQPVKGLVVELAAKALAGLPIPKAMRWGNSDIQFIRPVKTLTILLGDELIEGTILGVA
CCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
STRTIRGHRFMGESEFTIDSADQYPAILEERGKVMADYDARKAIILAGAKKAAEAVGGIA
CCCEECCCCCCCCCCCEECCCCCCHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHCCHH
DLEDELVEEVTSLVEWPVVLTAKFEQEFLNVPSEALVYTMKGDQKYFPVYDQEKNLLPNF
HHHHHHHHHHHHHHHCCHHEEEHHHHHHHCCCCHHEEEEECCCCEECCCCCCCCCCCCCE
IFVTNIESKEPRHIIEGNEKVVRPRLADAEFFFNTDRKRPLIDRLPELEQAIFQKQLGTI
EEEEECCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCCHHHHCHHHHHHHHHHHHCCH
KDKTDRITELAGYIAEQIGADVEKSQRAGLLAKCDLMTSMVFEFTDTQGVMGMHYATHDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EDAQVALALYEQYMPRFAGDDLPSTDVSASVAMADKLDTLVGIFGIGQAPKGSDPFALRR
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AALGILRIIVEKGYNLDLVDLVAKAQSLFGDKLTNANVDTDVIDFMLGRFRAWYQDEGFS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VDIIQAVLARRPTKPADFDQRVKAVSHFRELDAAESLAAANKRVGNILAKFDGELAQEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LALLQEDAEKVLAEKVEILAEALEPVFIAGNYQEALSRLAELREPVDAFFDGVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
ALKLNRLTLLNKLRNLFLDIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA