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The map label for this gene is malS [H]

Identifier: 209696360

GI number: 209696360

Start: 3212151

End: 3213815

Strand: Direct

Name: malS [H]

Synonym: VSAL_I2966

Alternate gene names: 209696360

Gene position: 3212151-3213815 (Clockwise)

Preceding gene: 209696359

Following gene: 209696361

Centisome position: 96.6

GC content: 42.22

Gene sequence:

>1665_bases
ATGAAATTATCTGTTTGTGCAACGTTTATCTCCCTTTCGCTATTGGTAGGATGTCAAAGTACGTCATTAACCTCGACAGA
TAGTTCTGATTCAATATCAACAGCTGATTCATCAAATCAAATTGAATCAACCACAAGCTCAAACAATAACACCGCTATTT
GTCCTGTATTAGGCATTCAACCAGTGACTGTTTCTGTGTCTGATTTATGGGCAAATGGTGATGTGATCACCGATGCTTAT
ACTGGGAATATTGCTCAGGTGGTTAATGGAGAGATAACGCTAACACCAAGTCGCCACAGTGGTGGGGTATTATTGTTGGA
ATCAGAAGAAGAAAAAGCGCGCTTCTCTTGGGATGGTGCGACGTTCTACCACATCGCAACGGATAGATTCTTTAATGGGA
ACAGTTGGAATGATCACAGTTATGGACGCCAAAGCACAAAAGAGGGTTTTCAGGGCGGAGATATTGTTGGCTTAACAAAA
AAACTCGATTACTTAGCCGAATTAGGCGTTGATGTCGTATGGATCTCCACTCCTCTAGAGCAAGTGCATGGGTGGTTACC
CAATAAGAGCGGCAATTCTCCTGAATACCCTTATGCCGGTGGTGCGACTCTCGATTGGACGCAACTGGATACCAACATGG
GCAGTGAGCAAGAAATGCGAACGTTTGTCGACCTAGCGCACCAATTGGGTATGCGAGTGATGTGGAGTGTGGATACGGCA
CCGCCAACACCAACCTTATCGGATTTACAGCAATTTTCAATTCAGCCGAATAATCCGGATGCGTTACCAAGCTATTGGAG
TGAATGGCAACCACAAGAAGGTCAAAACTTAACACATTATTTAGATGCTTTTAAAAATAAGAATGGTGAGATACCGGATT
GGTGGTCAGAAGCATGGATGGAAGAAAATAATGAAAACGCGATCACGTTGCCTGCGTTTTTTGCGAATAAGCCGTCAACT
AAAGTAAAAAGCAGTGAAAAGACAAAAAATGAGTACCTAGCTGGATGGATGAGTGCATGGGTGTCAGAGTTTGGGATTGA
TGGTTTTGTTGTTTCTAATTCAACACCAGAGTTAACTCAGAAGATCGAGCAAGAAGGGAAAGACGCTTTTACCCTGTGGA
ACAAAGACAACGCGTACAAAGCGTTAGAAAATGCGTCTTTTCGTGTGATTGATTTACAGCAACAGAATTACTTTAGCGAT
CAAATGCTGTCTTTAAAAAGCAATGATGAACCGCACTGTCTCACTAAGCTTAATGCTGAGTACGTAACTCAAAGCCAAGC
TGAAAAACCAAGTATGACTCGATTGACTTGGTTTGATGGTAAATCGTTATCTGATTCTTCAGTGGGCAGTGTAAACGCAG
ATAATTACGCGGATTACCGTGATGCAGCAACGGCGTTACTACTTTCTCCTAATGCGGTTTCTATTTGGTATGGGGATGAA
ACGGCCAAAATGGGAGGGATATACAGTAAGATGAATTGGAATGAGATAAAAGATCAACGTTGGTCGTTGCAGTCTCATTG
GAAAAAGCTCATCGAATTTAGAAATAATCACTCATCAATTGCGAATGGTGAGCATCAAGTGATCAAGCGTGAGCCTTACT
ATGCGTTTATTCGAAAGAATGAATCAGACAGTGTCATGGTGGTGTATACCGGTGAGAAAAAGTAG

Upstream 100 bases:

>100_bases
TTTTGTCTAAATATCGTGTTTTCGAAAATTAGAGGTTAATTATATCGATAACAGTGACTACAATTGGCACTCTTCTCTTT
TATTTAGGCTGCTGATTTCG

Downstream 100 bases:

>100_bases
TTTTTTTACTGTCATTAAAGTTTTACAAAATACCGGAGTAGAGTGTGATAATCGCCACGTTGGTTAAGTAAATAAGGTGG
TAATCTACGGTTAGGATTTG

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKLSVCATFISLSLLVGCQSTSLTSTDSSDSISTADSSNQIESTTSSNNNTAICPVLGIQPVTVSVSDLWANGDVITDAY
TGNIAQVVNGEITLTPSRHSGGVLLLESEEEKARFSWDGATFYHIATDRFFNGNSWNDHSYGRQSTKEGFQGGDIVGLTK
KLDYLAELGVDVVWISTPLEQVHGWLPNKSGNSPEYPYAGGATLDWTQLDTNMGSEQEMRTFVDLAHQLGMRVMWSVDTA
PPTPTLSDLQQFSIQPNNPDALPSYWSEWQPQEGQNLTHYLDAFKNKNGEIPDWWSEAWMEENNENAITLPAFFANKPST
KVKSSEKTKNEYLAGWMSAWVSEFGIDGFVVSNSTPELTQKIEQEGKDAFTLWNKDNAYKALENASFRVIDLQQQNYFSD
QMLSLKSNDEPHCLTKLNAEYVTQSQAEKPSMTRLTWFDGKSLSDSSVGSVNADNYADYRDAATALLLSPNAVSIWYGDE
TAKMGGIYSKMNWNEIKDQRWSLQSHWKKLIEFRNNHSSIANGEHQVIKREPYYAFIRKNESDSVMVVYTGEKK

Sequences:

>Translated_554_residues
MKLSVCATFISLSLLVGCQSTSLTSTDSSDSISTADSSNQIESTTSSNNNTAICPVLGIQPVTVSVSDLWANGDVITDAY
TGNIAQVVNGEITLTPSRHSGGVLLLESEEEKARFSWDGATFYHIATDRFFNGNSWNDHSYGRQSTKEGFQGGDIVGLTK
KLDYLAELGVDVVWISTPLEQVHGWLPNKSGNSPEYPYAGGATLDWTQLDTNMGSEQEMRTFVDLAHQLGMRVMWSVDTA
PPTPTLSDLQQFSIQPNNPDALPSYWSEWQPQEGQNLTHYLDAFKNKNGEIPDWWSEAWMEENNENAITLPAFFANKPST
KVKSSEKTKNEYLAGWMSAWVSEFGIDGFVVSNSTPELTQKIEQEGKDAFTLWNKDNAYKALENASFRVIDLQQQNYFSD
QMLSLKSNDEPHCLTKLNAEYVTQSQAEKPSMTRLTWFDGKSLSDSSVGSVNADNYADYRDAATALLLSPNAVSIWYGDE
TAKMGGIYSKMNWNEIKDQRWSLQSHWKKLIEFRNNHSSIANGEHQVIKREPYYAFIRKNESDSVMVVYTGEKK
>Mature_554_residues
MKLSVCATFISLSLLVGCQSTSLTSTDSSDSISTADSSNQIESTTSSNNNTAICPVLGIQPVTVSVSDLWANGDVITDAY
TGNIAQVVNGEITLTPSRHSGGVLLLESEEEKARFSWDGATFYHIATDRFFNGNSWNDHSYGRQSTKEGFQGGDIVGLTK
KLDYLAELGVDVVWISTPLEQVHGWLPNKSGNSPEYPYAGGATLDWTQLDTNMGSEQEMRTFVDLAHQLGMRVMWSVDTA
PPTPTLSDLQQFSIQPNNPDALPSYWSEWQPQEGQNLTHYLDAFKNKNGEIPDWWSEAWMEENNENAITLPAFFANKPST
KVKSSEKTKNEYLAGWMSAWVSEFGIDGFVVSNSTPELTQKIEQEGKDAFTLWNKDNAYKALENASFRVIDLQQQNYFSD
QMLSLKSNDEPHCLTKLNAEYVTQSQAEKPSMTRLTWFDGKSLSDSSVGSVNADNYADYRDAATALLLSPNAVSIWYGDE
TAKMGGIYSKMNWNEIKDQRWSLQSHWKKLIEFRNNHSSIANGEHQVIKREPYYAFIRKNESDSVMVVYTGEKK

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=569, Percent_Identity=33.7434094903339, Blast_Score=281, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 61960; Mature: 61960

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVCATFISLSLLVGCQSTSLTSTDSSDSISTADSSNQIESTTSSNNNTAICPVLGIQ
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
PVTVSVSDLWANGDVITDAYTGNIAQVVNGEITLTPSRHSGGVLLLESEEEKARFSWDGA
EEEEEEEHEECCCCEEEECCCCCCHHHCCCEEEEECCCCCCCEEEEECCCCCCEEEECCE
TFYHIATDRFFNGNSWNDHSYGRQSTKEGFQGGDIVGLTKKLDYLAELGVDVVWISTPLE
EEEEEEECEEECCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCHH
QVHGWLPNKSGNSPEYPYAGGATLDWTQLDTNMGSEQEMRTFVDLAHQLGMRVMWSVDTA
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
PPTPTLSDLQQFSIQPNNPDALPSYWSEWQPQEGQNLTHYLDAFKNKNGEIPDWWSEAWM
CCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
EENNENAITLPAFFANKPSTKVKSSEKTKNEYLAGWMSAWVSEFGIDGFVVSNSTPELTQ
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
KIEQEGKDAFTLWNKDNAYKALENASFRVIDLQQQNYFSDQMLSLKSNDEPHCLTKLNAE
HHHHCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEEECCHH
YVTQSQAEKPSMTRLTWFDGKSLSDSSVGSVNADNYADYRDAATALLLSPNAVSIWYGDE
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCEEEEEECCC
TAKMGGIYSKMNWNEIKDQRWSLQSHWKKLIEFRNNHSSIANGEHQVIKREPYYAFIRKN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCEEEEEEEC
ESDSVMVVYTGEKK
CCCCEEEEEECCCC
>Mature Secondary Structure
MKLSVCATFISLSLLVGCQSTSLTSTDSSDSISTADSSNQIESTTSSNNNTAICPVLGIQ
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCC
PVTVSVSDLWANGDVITDAYTGNIAQVVNGEITLTPSRHSGGVLLLESEEEKARFSWDGA
EEEEEEEHEECCCCEEEECCCCCCHHHCCCEEEEECCCCCCCEEEEECCCCCCEEEECCE
TFYHIATDRFFNGNSWNDHSYGRQSTKEGFQGGDIVGLTKKLDYLAELGVDVVWISTPLE
EEEEEEECEEECCCCCCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCHH
QVHGWLPNKSGNSPEYPYAGGATLDWTQLDTNMGSEQEMRTFVDLAHQLGMRVMWSVDTA
HHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
PPTPTLSDLQQFSIQPNNPDALPSYWSEWQPQEGQNLTHYLDAFKNKNGEIPDWWSEAWM
CCCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
EENNENAITLPAFFANKPSTKVKSSEKTKNEYLAGWMSAWVSEFGIDGFVVSNSTPELTQ
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
KIEQEGKDAFTLWNKDNAYKALENASFRVIDLQQQNYFSDQMLSLKSNDEPHCLTKLNAE
HHHHCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEEECCHH
YVTQSQAEKPSMTRLTWFDGKSLSDSSVGSVNADNYADYRDAATALLLSPNAVSIWYGDE
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCEEEEECCCEEEEEECCC
TAKMGGIYSKMNWNEIKDQRWSLQSHWKKLIEFRNNHSSIANGEHQVIKREPYYAFIRKN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCEEEEEEEC
ESDSVMVVYTGEKK
CCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]