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The map label for this gene is epsF [H]

Identifier: 209696320

GI number: 209696320

Start: 3163698

End: 3164915

Strand: Reverse

Name: epsF [H]

Synonym: VSAL_I2922

Alternate gene names: 209696320

Gene position: 3164915-3163698 (Counterclockwise)

Preceding gene: 209696321

Following gene: 209696319

Centisome position: 95.18

GC content: 41.05

Gene sequence:

>1218_bases
ATGGCAGCCTTTGAATACAAAGCCCTCGAAAAAAATGGCAAACAGAAAAAAGGCGTAATGGAGGGCGATAATGCTCGCCA
AGTTCGCCAGCGTTTAAAAGAGCAAGGGTTAATTCCTGTTGAAGTCATTGAAACAAAATCAAAACAGCAAAAATCCGCTT
CTCAAGGGTTGAGTTTTAAGCGCGGCATCAGTGTGAATGATCTGTCGTTAATTACTCGTCAACTGGCGACGTTAGTTCAG
GCGGGTATGCCATTAGAAGAGTGTTTAAAAGCCGTTGCTGAGCAAGGTGAAAAACCGCATATTCGCAGTATGATGATGGG
CGTAAGATCAAAAGTAATTGAAGGTTATCCATTAGCTGAAAGCTTTGGTGGATACCCTCATGTGTTTGATGATTTATTTT
GTTCAATGGTGGCTGCGGGTGAAAAATCGGGTCACTTAGATACCGTATTAAATCGCTTAGCGGATTACGCTGAAAATCGT
CAAAAAATGCGCAGTAAATTACAACAAGCGATGATTTATCCGATCATGCTAACGTTAATTGCTGTGGCTGTTATCTCATT
CTTATTAGCGACCGTTGTGCCTAAAATTGTCGATCAATTTGTGCAAATGGGGCAAGGGCTTCCTGCCGTCACAGAGATAC
TTATCGCGGCCAGTAATTTTGTTGTTCACTGGGGTGTTGTTGTGGTGGTTGTCATTGTTGCGATTATTTTTGGGATCAAA
TGGCTATTAACGAAGCCTCATTTACGTTTGGCGTGGGATAAAAAGATCTTACGATTGCCTGTGGTTGGTAAGGTGGTTCG
TGGTCTAAATACCTCTCGTTTTGCTCGTACTCTGTCTATTTGTACTTCCAGTGCGATCCCTTTATTAGATGGGATGAAAG
TTGCGGCAGACGTAATGAGTAATAAATACTTTAAACAGCAAGTGCTTGAAGCTGCAGATAATGTACGTGAAGGGGCAAGT
TTGCGTATTTCTTTAGAGCAAACCAAATTGTTCCCCCCAATGATGTTACATATGATAGCCAGTGGTGAGCAAAGTGGAGA
GTTAGAGCAAATGCTAACGCGTTCTGCGGATAACCAAGACCGAGATTTTGAATCTTTAGTGAATATGGCTTTAGGTATTT
TTGAGCCATTATTAATTGTCTTTATGGCAGGTATTGTACTTTTCATTGTTGTTGCGACACTTATGCCAATAATCGAATTA
AACAATTTAGTTGGATAG

Upstream 100 bases:

>100_bases
ACGTAAAAATACTCGCGGTATTCAACAAGATGGTTTATTGAAAGTACAAAAAGGCATTACGACATTAGAAGAGGTGATGC
GAGTCACTCGGGAAGGATAA

Downstream 100 bases:

>100_bases
TGAATAGACACTATCCTTTTCTTTTTTATTACTATGTTGAATTTTTAACGTCAGGAGTCATTCATGCAAGTCAATTCCGC
TCAACGTAAACAAGGTGGCT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAAFEYKALEKNGKQKKGVMEGDNARQVRQRLKEQGLIPVEVIETKSKQQKSASQGLSFKRGISVNDLSLITRQLATLVQ
AGMPLEECLKAVAEQGEKPHIRSMMMGVRSKVIEGYPLAESFGGYPHVFDDLFCSMVAAGEKSGHLDTVLNRLADYAENR
QKMRSKLQQAMIYPIMLTLIAVAVISFLLATVVPKIVDQFVQMGQGLPAVTEILIAASNFVVHWGVVVVVVIVAIIFGIK
WLLTKPHLRLAWDKKILRLPVVGKVVRGLNTSRFARTLSICTSSAIPLLDGMKVAADVMSNKYFKQQVLEAADNVREGAS
LRISLEQTKLFPPMMLHMIASGEQSGELEQMLTRSADNQDRDFESLVNMALGIFEPLLIVFMAGIVLFIVVATLMPIIEL
NNLVG

Sequences:

>Translated_405_residues
MAAFEYKALEKNGKQKKGVMEGDNARQVRQRLKEQGLIPVEVIETKSKQQKSASQGLSFKRGISVNDLSLITRQLATLVQ
AGMPLEECLKAVAEQGEKPHIRSMMMGVRSKVIEGYPLAESFGGYPHVFDDLFCSMVAAGEKSGHLDTVLNRLADYAENR
QKMRSKLQQAMIYPIMLTLIAVAVISFLLATVVPKIVDQFVQMGQGLPAVTEILIAASNFVVHWGVVVVVVIVAIIFGIK
WLLTKPHLRLAWDKKILRLPVVGKVVRGLNTSRFARTLSICTSSAIPLLDGMKVAADVMSNKYFKQQVLEAADNVREGAS
LRISLEQTKLFPPMMLHMIASGEQSGELEQMLTRSADNQDRDFESLVNMALGIFEPLLIVFMAGIVLFIVVATLMPIIEL
NNLVG
>Mature_404_residues
AAFEYKALEKNGKQKKGVMEGDNARQVRQRLKEQGLIPVEVIETKSKQQKSASQGLSFKRGISVNDLSLITRQLATLVQA
GMPLEECLKAVAEQGEKPHIRSMMMGVRSKVIEGYPLAESFGGYPHVFDDLFCSMVAAGEKSGHLDTVLNRLADYAENRQ
KMRSKLQQAMIYPIMLTLIAVAVISFLLATVVPKIVDQFVQMGQGLPAVTEILIAASNFVVHWGVVVVVVIVAIIFGIKW
LLTKPHLRLAWDKKILRLPVVGKVVRGLNTSRFARTLSICTSSAIPLLDGMKVAADVMSNKYFKQQVLEAADNVREGASL
RISLEQTKLFPPMMLHMIASGEQSGELEQMLTRSADNQDRDFESLVNMALGIFEPLLIVFMAGIVLFIVVATLMPIIELN
NLVG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=406, Percent_Identity=43.5960591133005, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1786295, Length=406, Percent_Identity=23.8916256157635, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44708; Mature: 44576

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAFEYKALEKNGKQKKGVMEGDNARQVRQRLKEQGLIPVEVIETKSKQQKSASQGLSFK
CCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHH
RGISVNDLSLITRQLATLVQAGMPLEECLKAVAEQGEKPHIRSMMMGVRSKVIEGYPLAE
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHH
SFGGYPHVFDDLFCSMVAAGEKSGHLDTVLNRLADYAENRQKMRSKLQQAMIYPIMLTLI
HCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAVISFLLATVVPKIVDQFVQMGQGLPAVTEILIAASNFVVHWGVVVVVVIVAIIFGIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLLTKPHLRLAWDKKILRLPVVGKVVRGLNTSRFARTLSICTSSAIPLLDGMKVAADVMS
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NKYFKQQVLEAADNVREGASLRISLEQTKLFPPMMLHMIASGEQSGELEQMLTRSADNQD
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
RDFESLVNMALGIFEPLLIVFMAGIVLFIVVATLMPIIELNNLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]