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The map label for this gene is epsE [H]

Identifier: 209696321

GI number: 209696321

Start: 3164916

End: 3166415

Strand: Reverse

Name: epsE [H]

Synonym: VSAL_I2923

Alternate gene names: 209696321

Gene position: 3166415-3164916 (Counterclockwise)

Preceding gene: 209696322

Following gene: 209696320

Centisome position: 95.23

GC content: 42.53

Gene sequence:

>1500_bases
ATGGCGGACATGCTGTTTCCTCATTCTGTCGCGGTACTGCGTTTGCCTTTTTCATTTGCAAAAAGGCACTCAGTGGTTAT
TGAACTCAGACCTGAAGGTTGGGTGTTGTTCTATGTAACAACACCGTCACCGATGGTGTTGTGTGAAATTCGACGTGTAT
GTAAATCGGAATTTTCATTAAGCCAATTAGAAAAAGAAGAGTTCGATACGAAAGTGACTCAGGTATACCAACGTAATTCA
TCAGAAGCACAGCAATTGATGGAAGACATTGGGGCTGATAACGATGACTTCTTTTCGTTGGCTGAAGAACTGCCAGACAA
TGACGATTTATTAGAATCAGAAGATGATGCGCCCATTATTAAATTGATTAACGCCATGTTAGGTGAAGCAATCAAAGAAG
GGGCGTCGGATATTCACATTGAAACGTTCGAAAAAGTATTGTCGATTCGTTTTCGTGTCGATGGTATTTTGCGTGATGTC
ATTAGCCCAAGTCGTAAACTAGCGCCGTTATTGGTCTCTCGTGTGAAAGTGATGTCCAAGTTGGATATCGCCGAAAAACG
TGTCCCTCAAGATGGTCGTATTTCATTGCGTATCGGTGGACGAGCCGTCGATGTGCGTGTGTCTACCATGCCGTCGTCTC
ATGGTGAGCGTGTGGTAATGCGTTTATTGGATAAGAACGCCACTCGCCTTGATTTACACAGCTTAGGTATGACTGCGGAT
AATCATCAAAAATTCCAACATATCATTAAACGACCTCATGGCATTATTCTTGTTACTGGTCCAACTGGGTCTGGTAAATC
GACGACGTTATATGCAGGGTTAGGTGAATTAGACAGCACTCAATCAAACATATTAACGGTTGAAGACCCGATTGAATTTG
ATATTGATGGCATTGGTCAAACGCAAGTAAACCCAAAGGTTGATATGACGTTTGCTCGTGGTTTACGAGCGATTCTTCGT
CAAGATCCTGATGTGGTTATGATTGGTGAAATTCGAGATTTAGAAACCGCAGAAATTGCTGTTCAAGCGTCGTTAACGGG
TCACTTGGTGATGTCTACATTGCACACTAACACCGCTGTTGGTGCGATTACTCGTCTTCGAGATATGGGCATTGAGCCTT
TTCTTATCTCATCGTCTTTGCTTGGCGTTCTTGCTCAGCGCTTGGTTCGAACTCTATGTAAAGAGTGCAAGGCGCCTTAT
CAAGCGGATGATGAACAAAAGAAATTATTCTCTTTATTACCAGCGGATGACTTAGTGCTTTATAAGCCGAATGGTTGTGA
GCATTGTAATGGCAAAGGGTATCGTGGGCGTACTGGTATTCATGAATTGCTTATTGTTAATGAGAAAGTTCAGGAACTGA
TTCATAAAGAAGCCGGCGAGCAAGAGATCGAAAAAGAAGTACGTAAAAATACTCGCGGTATTCAACAAGATGGTTTATTG
AAAGTACAAAAAGGCATTACGACATTAGAAGAGGTGATGCGAGTCACTCGGGAAGGATAA

Upstream 100 bases:

>100_bases
TTCCGGTGTTACCAACATTTGATGAAGGCATAAAACACCCCGCTGAAATTCAAGCGTTCTTTGAACAGTTTGATGATGAA
CATAAGGATAAGGGAAAATA

Downstream 100 bases:

>100_bases
ATGGCAGCCTTTGAATACAAAGCCCTCGAAAAAAATGGCAAACAGAAAAAAGGCGTAATGGAGGGCGATAATGCTCGCCA
AGTTCGCCAGCGTTTAAAAG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MADMLFPHSVAVLRLPFSFAKRHSVVIELRPEGWVLFYVTTPSPMVLCEIRRVCKSEFSLSQLEKEEFDTKVTQVYQRNS
SEAQQLMEDIGADNDDFFSLAEELPDNDDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGILRDV
ISPSRKLAPLLVSRVKVMSKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAD
NHQKFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSTQSNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCKECKAPY
QADDEQKKLFSLLPADDLVLYKPNGCEHCNGKGYRGRTGIHELLIVNEKVQELIHKEAGEQEIEKEVRKNTRGIQQDGLL
KVQKGITTLEEVMRVTREG

Sequences:

>Translated_499_residues
MADMLFPHSVAVLRLPFSFAKRHSVVIELRPEGWVLFYVTTPSPMVLCEIRRVCKSEFSLSQLEKEEFDTKVTQVYQRNS
SEAQQLMEDIGADNDDFFSLAEELPDNDDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGILRDV
ISPSRKLAPLLVSRVKVMSKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAD
NHQKFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSTQSNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCKECKAPY
QADDEQKKLFSLLPADDLVLYKPNGCEHCNGKGYRGRTGIHELLIVNEKVQELIHKEAGEQEIEKEVRKNTRGIQQDGLL
KVQKGITTLEEVMRVTREG
>Mature_498_residues
ADMLFPHSVAVLRLPFSFAKRHSVVIELRPEGWVLFYVTTPSPMVLCEIRRVCKSEFSLSQLEKEEFDTKVTQVYQRNSS
EAQQLMEDIGADNDDFFSLAEELPDNDDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGILRDVI
SPSRKLAPLLVSRVKVMSKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTADN
HQKFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSTQSNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILRQ
DPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCKECKAPYQ
ADDEQKKLFSLLPADDLVLYKPNGCEHCNGKGYRGRTGIHELLIVNEKVQELIHKEAGEQEIEKEVRKNTRGIQQDGLLK
VQKGITTLEEVMRVTREG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=481, Percent_Identity=55.7172557172557, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=43.1168831168831, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=43.7869822485207, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55647; Mature: 55516

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADMLFPHSVAVLRLPFSFAKRHSVVIELRPEGWVLFYVTTPSPMVLCEIRRVCKSEFSL
CCCCCCCCCEEEEECCHHHHCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCH
SQLEKEEFDTKVTQVYQRNSSEAQQLMEDIGADNDDFFSLAEELPDNDDLLESEDDAPII
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
KLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGILRDVISPSRKLAPLLVSRVKVMSK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAD
HHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEHHHCCCCCC
NHQKFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSTQSNILTVEDPIEFDIDGIGQ
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCEECCCCCCC
TQVNPKVDMTFARGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAV
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECHHHHHHHHHHHCCHHH
GAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCKECKAPYQADDEQKKLFSLLPADDLVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
YKPNGCEHCNGKGYRGRTGIHELLIVNEKVQELIHKEAGEQEIEKEVRKNTRGIQQDGLL
ECCCCCCCCCCCCCCCCCCHHHHHEECHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCH
KVQKGITTLEEVMRVTREG
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADMLFPHSVAVLRLPFSFAKRHSVVIELRPEGWVLFYVTTPSPMVLCEIRRVCKSEFSL
CCCCCCCCEEEEECCHHHHCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHCCH
SQLEKEEFDTKVTQVYQRNSSEAQQLMEDIGADNDDFFSLAEELPDNDDLLESEDDAPII
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
KLINAMLGEAIKEGASDIHIETFEKVLSIRFRVDGILRDVISPSRKLAPLLVSRVKVMSK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTAD
HHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEHHHCCCCCC
NHQKFQHIIKRPHGIILVTGPTGSGKSTTLYAGLGELDSTQSNILTVEDPIEFDIDGIGQ
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCEECCCCCCC
TQVNPKVDMTFARGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVMSTLHTNTAV
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECHHHHHHHHHHHCCHHH
GAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCKECKAPYQADDEQKKLFSLLPADDLVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
YKPNGCEHCNGKGYRGRTGIHELLIVNEKVQELIHKEAGEQEIEKEVRKNTRGIQQDGLL
ECCCCCCCCCCCCCCCCCCHHHHHEECHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCH
KVQKGITTLEEVMRVTREG
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]