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The map label for this gene is epsK [H]

Identifier: 209696315

GI number: 209696315

Start: 3160504

End: 3161523

Strand: Reverse

Name: epsK [H]

Synonym: VSAL_I2917

Alternate gene names: 209696315

Gene position: 3161523-3160504 (Counterclockwise)

Preceding gene: 209696316

Following gene: 209696314

Centisome position: 95.08

GC content: 40.69

Gene sequence:

>1020_bases
ATGAACCGTTCTCATAAAAATCAAAGCGGAGTTGCGTTGATTGTTGTATTGCTTCTATTAGCGATAATGGTGACGATTGC
CGCTCAAATGTCAGAGCGTTTGTTTATTCAGTTCCATCGTGCTGAAAATCAGTTGAATCATCAACAAGCGTATTGGTATT
CCATTGGGGTTGAAGCACTAGCCAAGGCGGGTATTGAAGAAGCGTACAAAGACGGAGATACCATTAATTTAAGCCAGATG
TGGGCGACAGAAGAAACAACGTACCCATTAGATTACGGCGAAGCGTTTGGTGGAGTGAAAGATAAGCAAGCTTGTTTTAA
TTTAAATGCATTAAGTAATATAGAACCAAGTGCCGATCCGTCTGTACGTCCATTTTTAGTGACCGTATGGATGAATTTAT
TGGAATCTGTTGAGGTAGAAAATTATTTAGCGGAAGTGATCGCGGATTCTAGCTGGGAATACATGGATGCTGATGATGTG
GTTCGTTCGAGCTCTGGTGTTGAAGACAGTACGTATCAATCCTTTAAGCCTCCTTATTTAGCACCAAATGGATGGATTGC
AGATGTGAGTGAGTTACGAGCGATTAATGGTGTCACCGCTGAGGTTTTTAAAGCGGTATCGCCATTAACGTGTGCTATTC
CAACGGCGAACTGGTATTTGAATGTGAATACATTACAACCAGAGCAAGCAAACATTTTAGTGGCGTTATTTTCACCGAAT
TTAAGTGAAAGCGATGCTCAAGAGGTATTAGAAAACCGTCCGTTTGATGGCTGGGCAAGTGTTGAAGATTTTTTATCAGA
GTCGGCCATTAATCGAGTGGATGAGAATATAAAAACAAAAGCCAAACCGTATTTATCAATAGATAGTCAGTTTTTTGAGT
TAGATACTCAAGTATTAGTAGAAGAATCACGCGTACGTGTGCGTTCTTTATTGCACAGCAGTGATAAGAAAGTGGTGAAC
GTTATCCGTCGCCAATATGGAGGAATGAGTGAGCGAACATCTGATAATAAGGCTGAATAG

Upstream 100 bases:

>100_bases
ATGGGGATGTAGATCGAATTTATCTTACGTCAGGTTATCAATTGGATGAAAGCAACAAAAATGACGATGAGTCCAAGGGC
GGTTAATGAATAGAGTATTA

Downstream 100 bases:

>100_bases
TCAACCATCAGATCCCATTCAATGGATCGTCTGGTCACCAGAACAAAAAGAAATCATCGCTTCGGGCGAATTAGTTTCCT
CTGAAACGTTATCCTCGTTA

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Cholera toxin secretion protein epsK [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNRSHKNQSGVALIVVLLLLAIMVTIAAQMSERLFIQFHRAENQLNHQQAYWYSIGVEALAKAGIEEAYKDGDTINLSQM
WATEETTYPLDYGEAFGGVKDKQACFNLNALSNIEPSADPSVRPFLVTVWMNLLESVEVENYLAEVIADSSWEYMDADDV
VRSSSGVEDSTYQSFKPPYLAPNGWIADVSELRAINGVTAEVFKAVSPLTCAIPTANWYLNVNTLQPEQANILVALFSPN
LSESDAQEVLENRPFDGWASVEDFLSESAINRVDENIKTKAKPYLSIDSQFFELDTQVLVEESRVRVRSLLHSSDKKVVN
VIRRQYGGMSERTSDNKAE

Sequences:

>Translated_339_residues
MNRSHKNQSGVALIVVLLLLAIMVTIAAQMSERLFIQFHRAENQLNHQQAYWYSIGVEALAKAGIEEAYKDGDTINLSQM
WATEETTYPLDYGEAFGGVKDKQACFNLNALSNIEPSADPSVRPFLVTVWMNLLESVEVENYLAEVIADSSWEYMDADDV
VRSSSGVEDSTYQSFKPPYLAPNGWIADVSELRAINGVTAEVFKAVSPLTCAIPTANWYLNVNTLQPEQANILVALFSPN
LSESDAQEVLENRPFDGWASVEDFLSESAINRVDENIKTKAKPYLSIDSQFFELDTQVLVEESRVRVRSLLHSSDKKVVN
VIRRQYGGMSERTSDNKAE
>Mature_339_residues
MNRSHKNQSGVALIVVLLLLAIMVTIAAQMSERLFIQFHRAENQLNHQQAYWYSIGVEALAKAGIEEAYKDGDTINLSQM
WATEETTYPLDYGEAFGGVKDKQACFNLNALSNIEPSADPSVRPFLVTVWMNLLESVEVENYLAEVIADSSWEYMDADDV
VRSSSGVEDSTYQSFKPPYLAPNGWIADVSELRAINGVTAEVFKAVSPLTCAIPTANWYLNVNTLQPEQANILVALFSPN
LSESDAQEVLENRPFDGWASVEDFLSESAINRVDENIKTKAKPYLSIDSQFFELDTQVLVEESRVRVRSLLHSSDKKVVN
VIRRQYGGMSERTSDNKAE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=267, Percent_Identity=32.5842696629214, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 37921; Mature: 37921

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSHKNQSGVALIVVLLLLAIMVTIAAQMSERLFIQFHRAENQLNHQQAYWYSIGVEAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
AKAGIEEAYKDGDTINLSQMWATEETTYPLDYGEAFGGVKDKQACFNLNALSNIEPSADP
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCHHHHHCCCCCHHHHCCCCHHCCCCCCCCC
SVRPFLVTVWMNLLESVEVENYLAEVIADSSWEYMDADDVVRSSSGVEDSTYQSFKPPYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHCCCCCC
APNGWIADVSELRAINGVTAEVFKAVSPLTCAIPTANWYLNVNTLQPEQANILVALFSPN
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCEEEEEECCC
LSESDAQEVLENRPFDGWASVEDFLSESAINRVDENIKTKAKPYLSIDSQFFELDTQVLV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHH
EESRVRVRSLLHSSDKKVVNVIRRQYGGMSERTSDNKAE
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNRSHKNQSGVALIVVLLLLAIMVTIAAQMSERLFIQFHRAENQLNHQQAYWYSIGVEAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
AKAGIEEAYKDGDTINLSQMWATEETTYPLDYGEAFGGVKDKQACFNLNALSNIEPSADP
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCHHHHHCCCCCHHHHCCCCHHCCCCCCCCC
SVRPFLVTVWMNLLESVEVENYLAEVIADSSWEYMDADDVVRSSSGVEDSTYQSFKPPYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHCCCCCC
APNGWIADVSELRAINGVTAEVFKAVSPLTCAIPTANWYLNVNTLQPEQANILVALFSPN
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEECCCCCCCEEEEEECCC
LSESDAQEVLENRPFDGWASVEDFLSESAINRVDENIKTKAKPYLSIDSQFFELDTQVLV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHH
EESRVRVRSLLHSSDKKVVNVIRRQYGGMSERTSDNKAE
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]