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The map label for this gene is epsJ [H]

Identifier: 209696316

GI number: 209696316

Start: 3161538

End: 3162185

Strand: Reverse

Name: epsJ [H]

Synonym: VSAL_I2918

Alternate gene names: 209696316

Gene position: 3162185-3161538 (Counterclockwise)

Preceding gene: 209696317

Following gene: 209696315

Centisome position: 95.1

GC content: 39.97

Gene sequence:

>648_bases
ATGAATAAACGATCTCGAGGTTTTACGCTGATCGAGGTGATGGTCGCTATCTCTGTATTTGCCACATTAAGTTTTTCGGC
GTATCAAGTGGTGAATCAAGTACAGCGCAGTAATGCTCTCTCTTTGGAAAAAAGTGAACGAATTAAAGAATTACAGCGCA
GTTTGGTTTTTCTTGATAATGACTTTCGCCAAATTGTGGCGCGTCAATTCAGAAGCAATGGAGAAAAAGCAGATGGAAAG
TTAATTTCAGCCGATGACTATTTATTGGATTCAGAATCTCAAGGTATTTTGTTTGTTCGTTTAGGTTGGAAAAATCCACA
GGAAGCCTTCCCGCGAGGTGAGATCACCAAAGTGGGCTATCGCGTCGTTGAAAATAAACTTGAACGTTTATGGTGGCGTT
ATCCTGATACCCCTGTTGGTCAAAAGCCAGTGGTTACGCCATTAATCGATGGCGTCGAAGAGATTAAATTTGAATTTTAT
GACGGTAAAACATGGCAAAAGAAATGGCAGAAAAAGAATGAGCTGCCATTTGCTATTAAGCTTCTTATCGTGTTTGAAGA
TTATGGGGATGTAGATCGAATTTATCTTACGTCAGGTTATCAATTGGATGAAAGCAACAAAAATGACGATGAGTCCAAGG
GCGGTTAA

Upstream 100 bases:

>100_bases
ACGTCTAATATTTTAAAATCAGTGGACGTATCTGTAGCAACAACCAAAGACCAAAAATCCCCACTATTAACGGTAAGAAC
GTATGTTGCGCCGTAGTCAT

Downstream 100 bases:

>100_bases
TGAATAGAGTATTAATGAACCGTTCTCATAAAAATCAAAGCGGAGTTGCGTTGATTGTTGTATTGCTTCTATTAGCGATA
ATGGTGACGATTGCCGCTCA

Product: general secretion pathway protein J

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNKRSRGFTLIEVMVAISVFATLSFSAYQVVNQVQRSNALSLEKSERIKELQRSLVFLDNDFRQIVARQFRSNGEKADGK
LISADDYLLDSESQGILFVRLGWKNPQEAFPRGEITKVGYRVVENKLERLWWRYPDTPVGQKPVVTPLIDGVEEIKFEFY
DGKTWQKKWQKKNELPFAIKLLIVFEDYGDVDRIYLTSGYQLDESNKNDDESKGG

Sequences:

>Translated_215_residues
MNKRSRGFTLIEVMVAISVFATLSFSAYQVVNQVQRSNALSLEKSERIKELQRSLVFLDNDFRQIVARQFRSNGEKADGK
LISADDYLLDSESQGILFVRLGWKNPQEAFPRGEITKVGYRVVENKLERLWWRYPDTPVGQKPVVTPLIDGVEEIKFEFY
DGKTWQKKWQKKNELPFAIKLLIVFEDYGDVDRIYLTSGYQLDESNKNDDESKGG
>Mature_215_residues
MNKRSRGFTLIEVMVAISVFATLSFSAYQVVNQVQRSNALSLEKSERIKELQRSLVFLDNDFRQIVARQFRSNGEKADGK
LISADDYLLDSESQGILFVRLGWKNPQEAFPRGEITKVGYRVVENKLERLWWRYPDTPVGQKPVVTPLIDGVEEIKFEFY
DGKTWQKKWQKKNELPFAIKLLIVFEDYGDVDRIYLTSGYQLDESNKNDDESKGG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

Organism=Escherichia coli, GI1789728, Length=199, Percent_Identity=28.1407035175879, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 24915; Mature: 24915

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRSRGFTLIEVMVAISVFATLSFSAYQVVNQVQRSNALSLEKSERIKELQRSLVFLDN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
DFRQIVARQFRSNGEKADGKLISADDYLLDSESQGILFVRLGWKNPQEAFPRGEITKVGY
HHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCHHHHCCCCCHHHHHH
RVVENKLERLWWRYPDTPVGQKPVVTPLIDGVEEIKFEFYDGKTWQKKWQKKNELPFAIK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHEEEEECCCHHHHHHHHCCCCCEEEE
LLIVFEDYGDVDRIYLTSGYQLDESNKNDDESKGG
EEEEEECCCCCCEEEEECCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNKRSRGFTLIEVMVAISVFATLSFSAYQVVNQVQRSNALSLEKSERIKELQRSLVFLDN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
DFRQIVARQFRSNGEKADGKLISADDYLLDSESQGILFVRLGWKNPQEAFPRGEITKVGY
HHHHHHHHHHHCCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCHHHHCCCCCHHHHHH
RVVENKLERLWWRYPDTPVGQKPVVTPLIDGVEEIKFEFYDGKTWQKKWQKKNELPFAIK
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHEEEEECCCHHHHHHHHCCCCCEEEE
LLIVFEDYGDVDRIYLTSGYQLDESNKNDDESKGG
EEEEEECCCCCCEEEEECCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]