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The map label for this gene is vcmD [H]

Identifier: 209696290

GI number: 209696290

Start: 3136681

End: 3138012

Strand: Reverse

Name: vcmD [H]

Synonym: VSAL_I2891

Alternate gene names: 209696290

Gene position: 3138012-3136681 (Counterclockwise)

Preceding gene: 209696291

Following gene: 209696288

Centisome position: 94.37

GC content: 39.26

Gene sequence:

>1332_bases
ATGTGGCAAGTCTTAAAAAACCTTACATTACACCGACGAGTATTAGCTCTGGCTATTCCAATGGTGTTGTCGAATATTAC
AACGCCACTACTTGGATTAGTTGATGCTGCTGTTATTGGGCATTTAGATCAGGCGTGGTATTTAGGTGGGGTTGCGGTTG
GCGGCACGATGATCAGCGTGACTTTTTGGTTACTTGGTTTTTTGCGTATGGCAACCACAGGACTTTCTGCTCAGGCCTAT
GGTGCTGAAGATAAAAAATTATTAAGCCAAACCTTTTCTCAGGGTATTTTTCTAGCTTTATGTTTTTCATTCATTCTGCT
TATTTTTCATCAGCCTCTCTCCTACGCTATTTTTTCTTTCAGCAATGCAACTCCAGAAGTGAAGATGTATGCAGAGCAAT
ATTTCTCTATTCGAATCTGGAGTGCACCAGCGGCATTGAGTAATTTGGTCATCATGGGCTGGTTACTCGGGACTCAGAAT
GCTCGTTATCCAATGTGGTTAGTGATTATTACAAACAGCATCAATATCGTTCTCGATTTATTATTTGTTGTTGGGTTTAA
TTGGAAGGTTGAAGGGGCGGCATTTGCTTCTGTATTAGCCGACTATGCCGCTTTGTTACTTGGTTTATTTTTTGTCTTTA
AACAAAAAGAAACATTGTATTTACCTCGTTTCTTGATGCCTCTGTCTGAATTGTTACTTGGGTTTAAACGCTTATTTAAA
TTAAATCGAGATATTTTCTTACGATCATTATGCCTTCAAGCGTGCTTTACTTTCATGACATTTAAGGGGGCGTCATTAGG
TGTTGATATTGTGGCAGCAAATGCGGTATTGATGAGTTTTTTGATGATGATATCTTATGGCATGGATGGTTTTGCTTATG
CTATGGAAGCCATGGTGGGAAAAGCGATTGGTGCAAAAAGCAAAACTCAGTTATCAGAATCATTGATTGGAATTACCTTT
TGGAGCTTTGCCATTAGCTTATTATTAAGTGTGGCTTTTGGTGTGTTTGGTGCTGGTTTAATTGGGATGATTTCTTCAAT
CACAGAAGTGCAAAATACGGCGTTAATCTATTTACCTTGGCTTATTGCTATGCCGTTGATATCCATGTGGTGTTTTTTAC
TTGATGGTATTTTTGTTGGTGCGACCAAAGGGTCTGAGATGAGAAATTCCATGTTTATCGCGATGCTGACGTTTTTCGTT
GTGTGGTGGCTAATGACGCCTTATGGTAACCATGCGTTATGGGCTGCAATTATCTCGTTTATGGGGATGCGAGGTATAAG
TCTTGCGATTACTTTTTATGTTCAATGGAAAAAAGGAGTTTTCCTATCATAG

Upstream 100 bases:

>100_bases
GTGCATATGAATTGGTAAAACGAAGCTTAAATTTGAAAAATTTTTGTATTGAGTCGAAGTAAGTAATAATGCCGTCACTT
TCTTTGTTGAGTACAAATCT

Downstream 100 bases:

>100_bases
GCCAAATACTCTATCATATTGATTAAAAATAATAAAAAAGGGTTAAGTAAACACTTAACCCTTTTTAGTATTTATTATAT
TTGAGTGTTTAAAAAAGTCG

Product: multidrug efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MWQVLKNLTLHRRVLALAIPMVLSNITTPLLGLVDAAVIGHLDQAWYLGGVAVGGTMISVTFWLLGFLRMATTGLSAQAY
GAEDKKLLSQTFSQGIFLALCFSFILLIFHQPLSYAIFSFSNATPEVKMYAEQYFSIRIWSAPAALSNLVIMGWLLGTQN
ARYPMWLVIITNSINIVLDLLFVVGFNWKVEGAAFASVLADYAALLLGLFFVFKQKETLYLPRFLMPLSELLLGFKRLFK
LNRDIFLRSLCLQACFTFMTFKGASLGVDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKSKTQLSESLIGITF
WSFAISLLLSVAFGVFGAGLIGMISSITEVQNTALIYLPWLIAMPLISMWCFLLDGIFVGATKGSEMRNSMFIAMLTFFV
VWWLMTPYGNHALWAAIISFMGMRGISLAITFYVQWKKGVFLS

Sequences:

>Translated_443_residues
MWQVLKNLTLHRRVLALAIPMVLSNITTPLLGLVDAAVIGHLDQAWYLGGVAVGGTMISVTFWLLGFLRMATTGLSAQAY
GAEDKKLLSQTFSQGIFLALCFSFILLIFHQPLSYAIFSFSNATPEVKMYAEQYFSIRIWSAPAALSNLVIMGWLLGTQN
ARYPMWLVIITNSINIVLDLLFVVGFNWKVEGAAFASVLADYAALLLGLFFVFKQKETLYLPRFLMPLSELLLGFKRLFK
LNRDIFLRSLCLQACFTFMTFKGASLGVDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKSKTQLSESLIGITF
WSFAISLLLSVAFGVFGAGLIGMISSITEVQNTALIYLPWLIAMPLISMWCFLLDGIFVGATKGSEMRNSMFIAMLTFFV
VWWLMTPYGNHALWAAIISFMGMRGISLAITFYVQWKKGVFLS
>Mature_443_residues
MWQVLKNLTLHRRVLALAIPMVLSNITTPLLGLVDAAVIGHLDQAWYLGGVAVGGTMISVTFWLLGFLRMATTGLSAQAY
GAEDKKLLSQTFSQGIFLALCFSFILLIFHQPLSYAIFSFSNATPEVKMYAEQYFSIRIWSAPAALSNLVIMGWLLGTQN
ARYPMWLVIITNSINIVLDLLFVVGFNWKVEGAAFASVLADYAALLLGLFFVFKQKETLYLPRFLMPLSELLLGFKRLFK
LNRDIFLRSLCLQACFTFMTFKGASLGVDIVAANAVLMSFLMMISYGMDGFAYAMEAMVGKAIGAKSKTQLSESLIGITF
WSFAISLLLSVAFGVFGAGLIGMISSITEVQNTALIYLPWLIAMPLISMWCFLLDGIFVGATKGSEMRNSMFIAMLTFFV
VWWLMTPYGNHALWAAIISFMGMRGISLAITFYVQWKKGVFLS

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=433, Percent_Identity=46.189376443418, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49156; Mature: 49156

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWQVLKNLTLHRRVLALAIPMVLSNITTPLLGLVDAAVIGHLDQAWYLGGVAVGGTMISV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TFWLLGFLRMATTGLSAQAYGAEDKKLLSQTFSQGIFLALCFSFILLIFHQPLSYAIFSF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
SNATPEVKMYAEQYFSIRIWSAPAALSNLVIMGWLLGTQNARYPMWLVIITNSINIVLDL
CCCCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHH
LFVVGFNWKVEGAAFASVLADYAALLLGLFFVFKQKETLYLPRFLMPLSELLLGFKRLFK
HHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LNRDIFLRSLCLQACFTFMTFKGASLGVDIVAANAVLMSFLMMISYGMDGFAYAMEAMVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KAIGAKSKTQLSESLIGITFWSFAISLLLSVAFGVFGAGLIGMISSITEVQNTALIYLPW
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LIAMPLISMWCFLLDGIFVGATKGSEMRNSMFIAMLTFFVVWWLMTPYGNHALWAAIISF
HHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MGMRGISLAITFYVQWKKGVFLS
HCCCCCEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MWQVLKNLTLHRRVLALAIPMVLSNITTPLLGLVDAAVIGHLDQAWYLGGVAVGGTMISV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TFWLLGFLRMATTGLSAQAYGAEDKKLLSQTFSQGIFLALCFSFILLIFHQPLSYAIFSF
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
SNATPEVKMYAEQYFSIRIWSAPAALSNLVIMGWLLGTQNARYPMWLVIITNSINIVLDL
CCCCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHH
LFVVGFNWKVEGAAFASVLADYAALLLGLFFVFKQKETLYLPRFLMPLSELLLGFKRLFK
HHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LNRDIFLRSLCLQACFTFMTFKGASLGVDIVAANAVLMSFLMMISYGMDGFAYAMEAMVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KAIGAKSKTQLSESLIGITFWSFAISLLLSVAFGVFGAGLIGMISSITEVQNTALIYLPW
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LIAMPLISMWCFLLDGIFVGATKGSEMRNSMFIAMLTFFVVWWLMTPYGNHALWAAIISF
HHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MGMRGISLAITFYVQWKKGVFLS
HCCCCCEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]