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The map label for this gene is birA [H]

Identifier: 209696275

GI number: 209696275

Start: 3116236

End: 3117195

Strand: Reverse

Name: birA [H]

Synonym: VSAL_I2876

Alternate gene names: 209696275

Gene position: 3117195-3116236 (Counterclockwise)

Preceding gene: 209696276

Following gene: 209696272

Centisome position: 93.75

GC content: 38.65

Gene sequence:

>960_bases
ATGAAGGAACACTTGAAAAAGCTAGAAATATTGAAGGCGCTCAGTGATGGTTCTTTTCATTCAGGTGAGGACTTAGGTGA
TGACATTGGCGTATCCAGAGCTGCCATAAGCAAGCATATCAAGGGGATTCAGGAGTGGGGATTATCAATATATAGAATTC
AGGGTAAAGGTTATGCTTTATCTTCTCCTTTAGAATTACTCTCAGTAGATCAGGTAATATGTAAGGATCTGCCACAAACT
TCAATTCTTGGTGTTGTTGATTCTACAAATCAGTTCTTATTGGACCAAATGACTGTGCTTAAAAAAGGGCAATCTTGCTT
TGCTGAATATCAAGTGGCAGGGCGAGGAAGGAGAGGTCGAACATGGGTATCTCCATTCGGTAGTAATATCTACTGTTCTA
TATTCTGGAGATTTGATAATGGACTTGCCGCAACCATGGGATTAAGTTTAGCAATTGGAATTGCTGTTGTTGACGCCCTA
GAAGTTATTGGTTGTAAAGGCTTAAAGCTGAAATGGCCGAATGATATTTATTGGAATGAATTAAAGTTAGCAGGTGTTCT
TATTGAGCTTTCCGGTCAATCAGGAGGAAGTGCAGATGTGGTTATTGGAATTGGCATCAATATAGAGTTGAATGAAAAAT
CTGCTTTAGTGATTGATCAACCTTGGGTTGATTTGATGACTATATTAGGTGAAAGGAACATCAGGAATAAGCTTGCGAGT
GAATTATTAGTATCCTTAAATAAAACATTACTTGAGTTTGAGCATTCGGGGCTACAGTCTTTCATCAACCGATGGAAAGA
ACTTGATAATTTTATAGATGAAAGCATCACGCTTACCATGGGTAGCCAATCTATTCATGGTGTTTGTCGAGGTATAAATT
CGCAAGGTGCGATATTGCTTGAACGTAACTGTATCGTCGAATCTTATTTCGGTGGTGAGATTTCAGTTAGAAAAGCGTGA

Upstream 100 bases:

>100_bases
ATGTTCAAAAAACAATTTTTGATATTTATCATATAGAGTTGGAACATGAAGTTCGATTTTATTTTTCGGGCAAAGAAACG
TCACTCTCGGAGTTGTTTGA

Downstream 100 bases:

>100_bases
TTATTTTTTTATCGATACGTATTCAATAGAATGATGTTCACCTTTCGTTAGAATTAAACTGGCTCTTTCTTTGGTAGGTA
ATATATTTTCAGTTAAATTT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKEHLKKLEILKALSDGSFHSGEDLGDDIGVSRAAISKHIKGIQEWGLSIYRIQGKGYALSSPLELLSVDQVICKDLPQT
SILGVVDSTNQFLLDQMTVLKKGQSCFAEYQVAGRGRRGRTWVSPFGSNIYCSIFWRFDNGLAATMGLSLAIGIAVVDAL
EVIGCKGLKLKWPNDIYWNELKLAGVLIELSGQSGGSADVVIGIGINIELNEKSALVIDQPWVDLMTILGERNIRNKLAS
ELLVSLNKTLLEFEHSGLQSFINRWKELDNFIDESITLTMGSQSIHGVCRGINSQGAILLERNCIVESYFGGEISVRKA

Sequences:

>Translated_319_residues
MKEHLKKLEILKALSDGSFHSGEDLGDDIGVSRAAISKHIKGIQEWGLSIYRIQGKGYALSSPLELLSVDQVICKDLPQT
SILGVVDSTNQFLLDQMTVLKKGQSCFAEYQVAGRGRRGRTWVSPFGSNIYCSIFWRFDNGLAATMGLSLAIGIAVVDAL
EVIGCKGLKLKWPNDIYWNELKLAGVLIELSGQSGGSADVVIGIGINIELNEKSALVIDQPWVDLMTILGERNIRNKLAS
ELLVSLNKTLLEFEHSGLQSFINRWKELDNFIDESITLTMGSQSIHGVCRGINSQGAILLERNCIVESYFGGEISVRKA
>Mature_319_residues
MKEHLKKLEILKALSDGSFHSGEDLGDDIGVSRAAISKHIKGIQEWGLSIYRIQGKGYALSSPLELLSVDQVICKDLPQT
SILGVVDSTNQFLLDQMTVLKKGQSCFAEYQVAGRGRRGRTWVSPFGSNIYCSIFWRFDNGLAATMGLSLAIGIAVVDAL
EVIGCKGLKLKWPNDIYWNELKLAGVLIELSGQSGGSADVVIGIGINIELNEKSALVIDQPWVDLMTILGERNIRNKLAS
ELLVSLNKTLLEFEHSGLQSFINRWKELDNFIDESITLTMGSQSIHGVCRGINSQGAILLERNCIVESYFGGEISVRKA

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=46.25, Blast_Score=317, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35002; Mature: 35002

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHLKKLEILKALSDGSFHSGEDLGDDIGVSRAAISKHIKGIQEWGLSIYRIQGKGYAL
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
SSPLELLSVDQVICKDLPQTSILGVVDSTNQFLLDQMTVLKKGQSCFAEYQVAGRGRRGR
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
TWVSPFGSNIYCSIFWRFDNGLAATMGLSLAIGIAVVDALEVIGCKGLKLKWPNDIYWNE
EEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCE
LKLAGVLIELSGQSGGSADVVIGIGINIELNEKSALVIDQPWVDLMTILGERNIRNKLAS
EEEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHCCCHHHHHHHH
ELLVSLNKTLLEFEHSGLQSFINRWKELDNFIDESITLTMGSQSIHGVCRGINSQGAILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEE
ERNCIVESYFGGEISVRKA
ECCEEEEEECCCEEEEECC
>Mature Secondary Structure
MKEHLKKLEILKALSDGSFHSGEDLGDDIGVSRAAISKHIKGIQEWGLSIYRIQGKGYAL
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHCCEEEEECCCCEEE
SSPLELLSVDQVICKDLPQTSILGVVDSTNQFLLDQMTVLKKGQSCFAEYQVAGRGRRGR
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
TWVSPFGSNIYCSIFWRFDNGLAATMGLSLAIGIAVVDALEVIGCKGLKLKWPNDIYWNE
EEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCE
LKLAGVLIELSGQSGGSADVVIGIGINIELNEKSALVIDQPWVDLMTILGERNIRNKLAS
EEEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCEEEEECCHHHHHHHHCCCHHHHHHHH
ELLVSLNKTLLEFEHSGLQSFINRWKELDNFIDESITLTMGSQSIHGVCRGINSQGAILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCEEE
ERNCIVESYFGGEISVRKA
ECCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]