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The map label for this gene is dusB [H]

Identifier: 209696242

GI number: 209696242

Start: 3077458

End: 3078426

Strand: Direct

Name: dusB [H]

Synonym: VSAL_I2838

Alternate gene names: 209696242

Gene position: 3077458-3078426 (Clockwise)

Preceding gene: 209696241

Following gene: 209696243

Centisome position: 92.55

GC content: 44.99

Gene sequence:

>969_bases
TTGCAGATAGGTAAATATACATTAAAGAACAATCTTATCGTTGCTCCTATGGCTGGTGTGACTGATAAACCTTTTCGTGA
GTTATGTATGCGTTACGGCGCAGGCATGGCTGTGAGCGAGATGATGTCTTCTAATCCAAAACTGTGGAAAACATCTAAGT
CCCAAAATCGCATGGTACATGAAGGTGAATCTGGTATTCGCTCGGTACAAATTGCAGGGGCAGATCCTAAGTTAATGGCG
GAAGCAGCGCGATACTGTGTTGAGACTGGTGCGCAAATCATCGATATCAATATGGGATGCCCTGCTAAGAAAGTGAATAA
GAAGCTTGCAGGCTCAGCGCTCCTACAACGCCCAGATTTGATTGAAGAGATCTTACTGGCAGTTGTGAAAGCGGTTGATG
TTCCTGTCACTCTGAAAACGCGTACAGGCTGGGATCCGGACAATAGAAACTGTGTTCAGGTTGCAAAAATGGCAGAACAA
TGCGGCATTCAAGCTTTAGCGCTCCATGGGCGTACCAGAGCTTGTATGTACAAAGGTGATGCTGAGTACGACAACATTAA
AGCGGTAAAAGAAGCGATTTCGATTCCGGTTATAGCAAACGGTGATATTGACTCACCGGAAAAGGCTCGTTTTGTGCTTG
ATTACACCGGCGCAGACGCTTTAATGATCGGCCGACCTGCACAAGGTAGGCCATGGATTTTTCAAGAAATCCAACATTAT
TTAGAAACGGGCGAATTCATGCCACCTCTTCCAATTGAAGAAGTGAAGGCCACTTTATTGGGTCATGTTCAAGCGCTTCA
CCTATTTTATGGTGAGTTTTTAGGCCCTCGCATTGCTCGTAAGCATGTGGGTTGGTATTTAAAAGAGCACGAACAAGCAA
ATGAATTCCGCCGCGGCTTTAATGCTATTGAAGATGCCCATCAGCAACTTCAACAGCTTGAAAGCTATTTCGACCACGTT
GCATCATAA

Upstream 100 bases:

>100_bases
TTACACAAACAATTTGTAAATGCTCAAAATTTGGTCTTTTCAGGCGAGGAAAAAATGCGTAAAATGCGCGCCCTTGCTGA
CACAAACGTGCGTGGATGTT

Downstream 100 bases:

>100_bases
TAACAGAAGAGCTAAACAGAATATGTTCGATCAATCTATGACTTCAGAAGCATTAACAGTTACAACAGTTACAGCACAAG
ACCAGATTACTCAGAAGCCA

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQIGKYTLKNNLIVAPMAGVTDKPFRELCMRYGAGMAVSEMMSSNPKLWKTSKSQNRMVHEGESGIRSVQIAGADPKLMA
EAARYCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVKAVDVPVTLKTRTGWDPDNRNCVQVAKMAEQ
CGIQALALHGRTRACMYKGDAEYDNIKAVKEAISIPVIANGDIDSPEKARFVLDYTGADALMIGRPAQGRPWIFQEIQHY
LETGEFMPPLPIEEVKATLLGHVQALHLFYGEFLGPRIARKHVGWYLKEHEQANEFRRGFNAIEDAHQQLQQLESYFDHV
AS

Sequences:

>Translated_322_residues
MQIGKYTLKNNLIVAPMAGVTDKPFRELCMRYGAGMAVSEMMSSNPKLWKTSKSQNRMVHEGESGIRSVQIAGADPKLMA
EAARYCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVKAVDVPVTLKTRTGWDPDNRNCVQVAKMAEQ
CGIQALALHGRTRACMYKGDAEYDNIKAVKEAISIPVIANGDIDSPEKARFVLDYTGADALMIGRPAQGRPWIFQEIQHY
LETGEFMPPLPIEEVKATLLGHVQALHLFYGEFLGPRIARKHVGWYLKEHEQANEFRRGFNAIEDAHQQLQQLESYFDHV
AS
>Mature_322_residues
MQIGKYTLKNNLIVAPMAGVTDKPFRELCMRYGAGMAVSEMMSSNPKLWKTSKSQNRMVHEGESGIRSVQIAGADPKLMA
EAARYCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDLIEEILLAVVKAVDVPVTLKTRTGWDPDNRNCVQVAKMAEQ
CGIQALALHGRTRACMYKGDAEYDNIKAVKEAISIPVIANGDIDSPEKARFVLDYTGADALMIGRPAQGRPWIFQEIQHY
LETGEFMPPLPIEEVKATLLGHVQALHLFYGEFLGPRIARKHVGWYLKEHEQANEFRRGFNAIEDAHQQLQQLESYFDHV
AS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=32.7659574468085, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=32.3529411764706, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=32.15859030837, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=33.201581027668, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=264, Percent_Identity=29.9242424242424, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=69.1588785046729, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1788462, Length=259, Percent_Identity=32.046332046332, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=325, Percent_Identity=23.6923076923077, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=241, Percent_Identity=31.9502074688797, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=33.4821428571429, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=265, Percent_Identity=30.188679245283, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=260, Percent_Identity=28.4615384615385, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=33.1896551724138, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=28.3524904214559, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=27.0742358078603, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=243, Percent_Identity=32.5102880658436, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24580595, Length=246, Percent_Identity=30.4878048780488, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=246, Percent_Identity=30.4878048780488, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=30.4878048780488, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=26.9911504424779, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35837; Mature: 35837

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGKYTLKNNLIVAPMAGVTDKPFRELCMRYGAGMAVSEMMSSNPKLWKTSKSQNRMVH
CCCCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCHHH
EGESGIRSVQIAGADPKLMAEAARYCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDL
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
IEEILLAVVKAVDVPVTLKTRTGWDPDNRNCVQVAKMAEQCGIQALALHGRTRACMYKGD
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEECCC
AEYDNIKAVKEAISIPVIANGDIDSPEKARFVLDYTGADALMIGRPAQGRPWIFQEIQHY
CCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LETGEFMPPLPIEEVKATLLGHVQALHLFYGEFLGPRIARKHVGWYLKEHEQANEFRRGF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAIEDAHQQLQQLESYFDHVAS
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGKYTLKNNLIVAPMAGVTDKPFRELCMRYGAGMAVSEMMSSNPKLWKTSKSQNRMVH
CCCCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCHHH
EGESGIRSVQIAGADPKLMAEAARYCVETGAQIIDINMGCPAKKVNKKLAGSALLQRPDL
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
IEEILLAVVKAVDVPVTLKTRTGWDPDNRNCVQVAKMAEQCGIQALALHGRTRACMYKGD
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEECCC
AEYDNIKAVKEAISIPVIANGDIDSPEKARFVLDYTGADALMIGRPAQGRPWIFQEIQHY
CCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LETGEFMPPLPIEEVKATLLGHVQALHLFYGEFLGPRIARKHVGWYLKEHEQANEFRRGF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAIEDAHQQLQQLESYFDHVAS
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]