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The map label for this gene is metL [H]

Identifier: 209696154

GI number: 209696154

Start: 2974647

End: 2977067

Strand: Direct

Name: metL [H]

Synonym: VSAL_I2748

Alternate gene names: 209696154

Gene position: 2974647-2977067 (Clockwise)

Preceding gene: 209696153

Following gene: 209696155

Centisome position: 89.46

GC content: 42.09

Gene sequence:

>2421_bases
ATGACAACGTCTTTGGTGCAACGTCAGTTACATAAATTTGGTGGCAGCAGTCTTTCTGATCCTGAGTGTTATCGCCGTGT
GGCAACCATCTTAAAAGAATATTCAAACGAGCATGATCTTGTTGTTGTTTCTGCCGCGGGTAATACAACAAACCGTATTC
TTGAATGGATTGATAACTTATCTAAAGATGGTCGTTTGGCTCACGATACATTGGAGTCTTTGCGTCATTATCAGCAACAG
TTAATTTCTGAATTGTTAGAGCCAGATCAGGCGGAAGTATTGCAGGCATTGTTGCATAAAGAGTTGATTGCAATCGGTCA
ATTACATGCTCCGCTAAATGAAGTTCAACAAGCCGATGTGCTTGGTCATGGTGAAATATGGTCGTCTCGTTTGTTGTCGG
CTTATTTAAACCAGATTGAATTAAAATCGGCACCGTTGGATTCTCGCCATTTTTTAAGAGCAGAAAGAGGGGCTCAGCCT
GAGGTTGATCGTGTTCTTTCATGGCCTTTATTTAAAGAACAAATAGCACAACACCCTCATGGCCGTCTTGTTATTACTGG
ATTTATGGCCCGTGACCAAAAGGGTGAAACCGTATTATTAGGGCGTAATGGATCGGATTACTCAGCAACTATGATTGGTG
CATTAGCTGAAGTTTCTCGTGTTACGATTTGGAGTGATGTTGCTGGGGTATTTAGTGCGGATCCTAGAAAAGTGGAAGAC
GCTTGTTTATTGCCGCTACTTCGTTTGGATGAAGCCAGTGAATTGGCTCGTTTAGCTGCCCCTGTTTTACATAGCAGAAC
ATTACAGCCAGTAGCGCAAAGTGCGTTAGATTTGAGTCTACGTTGCAGTTATCAACCAGAATCGGGTTCAACACGCATTG
AACGTGTCTTGGCCTCTGGTCGTGGCGCTAAAATTGTTACTTCGTTGGATGATGTGTACTTAATTCAACTTGGCTTTGGC
CGTGGGCAAGATTTTGAACGAGCTCAAACCGAGGTGCTTTCTCTTTTAACTCGTGCTCAACTTCAACCTCTTGCGTTAGA
AGCACAAGCCGATAAATTTACTTTATTACTTGCCTACACTTCAGAGATCGTCAGTGATGCTTTAGATATGCTGCAAAATA
CAGCGGTGCATGCTGAAATTAAATTAAAAGAAGGGTACTCAATGGTTGCAGCAGTAGGTGCTGGTGTAACGGCTAATGCC
ATTCATAGCCATGGTTTTTACCATCAATTAAAAAATAGACCTTTAGAGTTTGTGTGTGAATCGGAATCAGGGTTAAGTCT
TGTTGCTATTCTTCGTAAATTAGACACCGATGAATTGATCAATGAGATTCATACCAGTTTATTTCAAGCTCAGAAACGAG
TTGGCCTTGTTTTGTGTGGTCACGGTAATATTGGTGCGCAGTGGCTTACATTGTTTACTCAGCAAAAACGAATTTTAGAA
AAACGTCGAGGCATGATTTTTTCATTAGTCGGAGTCGTTGACAGTCATCGATACTGGTTCGATTTTGACGGTATCGATAG
TGCATCGGTAGTCAGCCATTTTGATGATGAAGCGGTGAGCTACGAAGAATTTGAATGGATTGAAAAAATCGGTTCAGTCC
AAGGATACGATGACGTTGTTATTTTAGATGTAACGGCAAGCTCTGAGCTTGCAAGTCAATACACTAAAATTGCAGAACAA
GGCATGCACTTGATCTCTGCAAATAAGGTCGCTGGTTCAGCACCAAGTCATGAATATCAAGCGGTTGTTGATGCGTTTGC
AAAAACCGGTCGACATTGGTTATACAATGCAACGGTTGGCGCGGGATTACCAATTAATCATACTGTGCAAGATTTGCGTG
AAAGCGGAGACAGCATTGTTGCACTCTCTGGGATCTTTTCTGGCACTCTTTCTTGGTTATTCCAACAATATACTGGAGAG
ACTCCCTTTACTGAGTTAGTCGATTTAGCATGGCAACAAGGATTGACAGAGCCAGATCCTAGGCATGATTTAGATGGTTC
CGATGTAATGAGAAAGCTTGTTATTTTGGCAAGAGAGTCAGGGTTACAAATCGAACCGAATCAAATCGAAGTCGAATCGT
TGGTTCCCGCCGAATTACAGCAAGTGAGTCTTGATGACTTCTTGGCAAAAGGTGATGCCTTAAATGAAGCATTATCGGAA
CGTTTGGAACAAGCGCAGCGAGAACAAAAAGTACTTCGTTATGTTGCCCGTTTGAATAAAAATGGCAGTGCAACGGTTGG
GATTGAAGCGCTGTCTGAACATCATGCATTGGCTAATTTATTACCGTGCGATAATATTTTTGCGATTGAAAGTCAGTGGT
ATAAAGATAACCCTTTAGTGATAAGAGGGCCAGGCGCCGGAAGAGAGGTTACCGCTGGAGCAATCCAGTCTGATTTGAAT
AAAATTTCAGCGTTATTTTAA

Upstream 100 bases:

>100_bases
TGGTGCCGACGTTATTACGTATTTCTGTTGGCCTAGAAGACAGTAGAGATTTAATCGCAGATCTTACACAAGCGTTTGCT
GTGGCTGCAGAGGTGAAATA

Downstream 100 bases:

>100_bases
CCAAATGTAATACTTATTAACGTTTCGTTATATTTTAAACCATGCCATGTTGCATGGTTTTTTTCGTTTAACGTAGGAAA
AATCGGAAACTATTGATATT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MTTSLVQRQLHKFGGSSLSDPECYRRVATILKEYSNEHDLVVVSAAGNTTNRILEWIDNLSKDGRLAHDTLESLRHYQQQ
LISELLEPDQAEVLQALLHKELIAIGQLHAPLNEVQQADVLGHGEIWSSRLLSAYLNQIELKSAPLDSRHFLRAERGAQP
EVDRVLSWPLFKEQIAQHPHGRLVITGFMARDQKGETVLLGRNGSDYSATMIGALAEVSRVTIWSDVAGVFSADPRKVED
ACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLDDVYLIQLGFG
RGQDFERAQTEVLSLLTRAQLQPLALEAQADKFTLLLAYTSEIVSDALDMLQNTAVHAEIKLKEGYSMVAAVGAGVTANA
IHSHGFYHQLKNRPLEFVCESESGLSLVAILRKLDTDELINEIHTSLFQAQKRVGLVLCGHGNIGAQWLTLFTQQKRILE
KRRGMIFSLVGVVDSHRYWFDFDGIDSASVVSHFDDEAVSYEEFEWIEKIGSVQGYDDVVILDVTASSELASQYTKIAEQ
GMHLISANKVAGSAPSHEYQAVVDAFAKTGRHWLYNATVGAGLPINHTVQDLRESGDSIVALSGIFSGTLSWLFQQYTGE
TPFTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILARESGLQIEPNQIEVESLVPAELQQVSLDDFLAKGDALNEALSE
RLEQAQREQKVLRYVARLNKNGSATVGIEALSEHHALANLLPCDNIFAIESQWYKDNPLVIRGPGAGREVTAGAIQSDLN
KISALF

Sequences:

>Translated_806_residues
MTTSLVQRQLHKFGGSSLSDPECYRRVATILKEYSNEHDLVVVSAAGNTTNRILEWIDNLSKDGRLAHDTLESLRHYQQQ
LISELLEPDQAEVLQALLHKELIAIGQLHAPLNEVQQADVLGHGEIWSSRLLSAYLNQIELKSAPLDSRHFLRAERGAQP
EVDRVLSWPLFKEQIAQHPHGRLVITGFMARDQKGETVLLGRNGSDYSATMIGALAEVSRVTIWSDVAGVFSADPRKVED
ACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLDDVYLIQLGFG
RGQDFERAQTEVLSLLTRAQLQPLALEAQADKFTLLLAYTSEIVSDALDMLQNTAVHAEIKLKEGYSMVAAVGAGVTANA
IHSHGFYHQLKNRPLEFVCESESGLSLVAILRKLDTDELINEIHTSLFQAQKRVGLVLCGHGNIGAQWLTLFTQQKRILE
KRRGMIFSLVGVVDSHRYWFDFDGIDSASVVSHFDDEAVSYEEFEWIEKIGSVQGYDDVVILDVTASSELASQYTKIAEQ
GMHLISANKVAGSAPSHEYQAVVDAFAKTGRHWLYNATVGAGLPINHTVQDLRESGDSIVALSGIFSGTLSWLFQQYTGE
TPFTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILARESGLQIEPNQIEVESLVPAELQQVSLDDFLAKGDALNEALSE
RLEQAQREQKVLRYVARLNKNGSATVGIEALSEHHALANLLPCDNIFAIESQWYKDNPLVIRGPGAGREVTAGAIQSDLN
KISALF
>Mature_805_residues
TTSLVQRQLHKFGGSSLSDPECYRRVATILKEYSNEHDLVVVSAAGNTTNRILEWIDNLSKDGRLAHDTLESLRHYQQQL
ISELLEPDQAEVLQALLHKELIAIGQLHAPLNEVQQADVLGHGEIWSSRLLSAYLNQIELKSAPLDSRHFLRAERGAQPE
VDRVLSWPLFKEQIAQHPHGRLVITGFMARDQKGETVLLGRNGSDYSATMIGALAEVSRVTIWSDVAGVFSADPRKVEDA
CLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLDDVYLIQLGFGR
GQDFERAQTEVLSLLTRAQLQPLALEAQADKFTLLLAYTSEIVSDALDMLQNTAVHAEIKLKEGYSMVAAVGAGVTANAI
HSHGFYHQLKNRPLEFVCESESGLSLVAILRKLDTDELINEIHTSLFQAQKRVGLVLCGHGNIGAQWLTLFTQQKRILEK
RRGMIFSLVGVVDSHRYWFDFDGIDSASVVSHFDDEAVSYEEFEWIEKIGSVQGYDDVVILDVTASSELASQYTKIAEQG
MHLISANKVAGSAPSHEYQAVVDAFAKTGRHWLYNATVGAGLPINHTVQDLRESGDSIVALSGIFSGTLSWLFQQYTGET
PFTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILARESGLQIEPNQIEVESLVPAELQQVSLDDFLAKGDALNEALSER
LEQAQREQKVLRYVARLNKNGSATVGIEALSEHHALANLLPCDNIFAIESQWYKDNPLVIRGPGAGREVTAGAIQSDLNK
ISALF

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=804, Percent_Identity=59.5771144278607, Blast_Score=959, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=839, Percent_Identity=28.3671036948748, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790455, Length=456, Percent_Identity=25.219298245614, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322599, Length=251, Percent_Identity=37.0517928286853, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320893, Length=359, Percent_Identity=26.7409470752089, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88964; Mature: 88832

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSLVQRQLHKFGGSSLSDPECYRRVATILKEYSNEHDLVVVSAAGNTTNRILEWIDNL
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
SKDGRLAHDTLESLRHYQQQLISELLEPDQAEVLQALLHKELIAIGQLHAPLNEVQQADV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
LGHGEIWSSRLLSAYLNQIELKSAPLDSRHFLRAERGAQPEVDRVLSWPLFKEQIAQHPH
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCC
GRLVITGFMARDQKGETVLLGRNGSDYSATMIGALAEVSRVTIWSDVAGVFSADPRKVED
CCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCC
RGAKIVTSLDDVYLIQLGFGRGQDFERAQTEVLSLLTRAQLQPLALEAQADKFTLLLAYT
CCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHH
SEIVSDALDMLQNTAVHAEIKLKEGYSMVAAVGAGVTANAIHSHGFYHQLKNRPLEFVCE
HHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEC
SESGLSLVAILRKLDTDELINEIHTSLFQAQKRVGLVLCGHGNIGAQWLTLFTQQKRILE
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
KRRGMIFSLVGVVDSHRYWFDFDGIDSASVVSHFDDEAVSYEEFEWIEKIGSVQGYDDVV
HHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHCCHHHHHHHHHHCCCCCCCCEE
ILDVTASSELASQYTKIAEQGMHLISANKVAGSAPSHEYQAVVDAFAKTGRHWLYNATVG
EEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
AGLPINHTVQDLRESGDSIVALSGIFSGTLSWLFQQYTGETPFTELVDLAWQQGLTEPDP
CCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
RHDLDGSDVMRKLVILARESGLQIEPNQIEVESLVPAELQQVSLDDFLAKGDALNEALSE
CCCCCHHHHHHHHHHHHHCCCCEECCCCEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHH
RLEQAQREQKVLRYVARLNKNGSATVGIEALSEHHALANLLPCDNIFAIESQWYKDNPLV
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
IRGPGAGREVTAGAIQSDLNKISALF
EECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSLVQRQLHKFGGSSLSDPECYRRVATILKEYSNEHDLVVVSAAGNTTNRILEWIDNL
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCC
SKDGRLAHDTLESLRHYQQQLISELLEPDQAEVLQALLHKELIAIGQLHAPLNEVQQADV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
LGHGEIWSSRLLSAYLNQIELKSAPLDSRHFLRAERGAQPEVDRVLSWPLFKEQIAQHPH
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCC
GRLVITGFMARDQKGETVLLGRNGSDYSATMIGALAEVSRVTIWSDVAGVFSADPRKVED
CCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ACLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCC
RGAKIVTSLDDVYLIQLGFGRGQDFERAQTEVLSLLTRAQLQPLALEAQADKFTLLLAYT
CCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEHHHHH
SEIVSDALDMLQNTAVHAEIKLKEGYSMVAAVGAGVTANAIHSHGFYHQLKNRPLEFVCE
HHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEC
SESGLSLVAILRKLDTDELINEIHTSLFQAQKRVGLVLCGHGNIGAQWLTLFTQQKRILE
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
KRRGMIFSLVGVVDSHRYWFDFDGIDSASVVSHFDDEAVSYEEFEWIEKIGSVQGYDDVV
HHCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHCCHHHHHHHHHHCCCCCCCCEE
ILDVTASSELASQYTKIAEQGMHLISANKVAGSAPSHEYQAVVDAFAKTGRHWLYNATVG
EEEECCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
AGLPINHTVQDLRESGDSIVALSGIFSGTLSWLFQQYTGETPFTELVDLAWQQGLTEPDP
CCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
RHDLDGSDVMRKLVILARESGLQIEPNQIEVESLVPAELQQVSLDDFLAKGDALNEALSE
CCCCCHHHHHHHHHHHHHCCCCEECCCCEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHH
RLEQAQREQKVLRYVARLNKNGSATVGIEALSEHHALANLLPCDNIFAIESQWYKDNPLV
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
IRGPGAGREVTAGAIQSDLNKISALF
EECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]