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The map label for this gene is leuO [H]

Identifier: 209696155

GI number: 209696155

Start: 2977409

End: 2978383

Strand: Direct

Name: leuO [H]

Synonym: VSAL_I2749

Alternate gene names: 209696155

Gene position: 2977409-2978383 (Clockwise)

Preceding gene: 209696154

Following gene: 209696156

Centisome position: 89.54

GC content: 38.87

Gene sequence:

>975_bases
ATGAGTATGTCTGAATTTTCCTCTGCATCTAATAGCGTTAGAGCAGAACAAGTATTACGTGGAGTAGATTTAAATCTACT
GACTGTTTTTGATGCAGTAATGCAAGAGCAAAATATTACTCGAGCAGCTCAAAACCTAGGTATGTCACAACCGGCAGTGA
GTAATGCGGTATCACGACTAAAAGTGATGTTTAATGATGAACTCTTTATTCGCCATGGTCGTGGTATTCAACCAACGCAA
CGTGCTCGTCAATTATTCGCACCAATGCGTCAAGCACTGCAATTGGTAAAAAATGAATTACCAAGTTCGACGTTTATTCC
TGAAACATCAATGAGACAATTTAAGCTCTCAATTTGTAGCCCTTCGGATATTCGTTTTGCTCCACGAATCATGAGAGATA
TTCAGCAAAAATCGCCTCATATTAGTTTAAAGTTGTCTGGTGAACATGAAGCGGATATTGCTCAAAAGCTTCGTTATCAA
GAAATTGACTTTGTGATTGATTATGTTCGTTTTGACGAACCTGGTTTTATGAGCACTGAGTTATTTACTGATGAATTAGT
GGTTGTTGCTGCCGTTAATCACCCTCGAATTCAGGGTATGATTACTAAAGAGCAATTGGCAAATGAGAAACATGCTGTTC
CTGCTCGTATTGAAAATACAAAAGGGTTTGCAGACCATTTATATCAAGATGCTATTTATGAGCAAGCTTACAAAGGGGCT
AGCATCAGCAACATTCTTTATGTTGTTTCTCAATCAGAGTTGATTACGGTTGCTCCTAAGTGGTTGGTCAGTTCAATGCC
AAACCGCGATCAACTGGCTATTCTTCCATTGCCAATTGAATCAAAAGGGATCAGTGGCTATTTAAGCTGGCATGACTCGT
ACCAAAAAGACAAAGGTCATATTTGGATGCGTGATCAATTGATGCTTATTTGTGGCGAAGAAGTCGTTGCTTCAAGTAAA
GGTATGATGTTTTAG

Upstream 100 bases:

>100_bases
GGCTAGCATACGTTCTGTCAATAACGTGTATAACTATAATAATATGCAGCTTACTTTTTCTTATTTTAATAATTTGCTGA
AAAATAGAGAGAGCCTTTAA

Downstream 100 bases:

>100_bases
CGTTGAAATACTCTAAAATTAGAAGAATAAAAGGCAGCATCATTGATGTTGCCTTTTTTGTAGGATCTCATTCGATATAA
ATGAAATGTATATTATTAAT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSMSEFSSASNSVRAEQVLRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKVMFNDELFIRHGRGIQPTQ
RARQLFAPMRQALQLVKNELPSSTFIPETSMRQFKLSICSPSDIRFAPRIMRDIQQKSPHISLKLSGEHEADIAQKLRYQ
EIDFVIDYVRFDEPGFMSTELFTDELVVVAAVNHPRIQGMITKEQLANEKHAVPARIENTKGFADHLYQDAIYEQAYKGA
SISNILYVVSQSELITVAPKWLVSSMPNRDQLAILPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSK
GMMF

Sequences:

>Translated_324_residues
MSMSEFSSASNSVRAEQVLRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKVMFNDELFIRHGRGIQPTQ
RARQLFAPMRQALQLVKNELPSSTFIPETSMRQFKLSICSPSDIRFAPRIMRDIQQKSPHISLKLSGEHEADIAQKLRYQ
EIDFVIDYVRFDEPGFMSTELFTDELVVVAAVNHPRIQGMITKEQLANEKHAVPARIENTKGFADHLYQDAIYEQAYKGA
SISNILYVVSQSELITVAPKWLVSSMPNRDQLAILPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSK
GMMF
>Mature_323_residues
SMSEFSSASNSVRAEQVLRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRLKVMFNDELFIRHGRGIQPTQR
ARQLFAPMRQALQLVKNELPSSTFIPETSMRQFKLSICSPSDIRFAPRIMRDIQQKSPHISLKLSGEHEADIAQKLRYQE
IDFVIDYVRFDEPGFMSTELFTDELVVVAAVNHPRIQGMITKEQLANEKHAVPARIENTKGFADHLYQDAIYEQAYKGAS
ISNILYVVSQSELITVAPKWLVSSMPNRDQLAILPLPIESKGISGYLSWHDSYQKDKGHIWMRDQLMLICGEEVVASSKG
MMF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=296, Percent_Identity=52.027027027027, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87081766, Length=267, Percent_Identity=26.2172284644195, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786820, Length=294, Percent_Identity=27.2108843537415, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=24.5733788395904, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36653; Mature: 36521

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSEFSSASNSVRAEQVLRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRL
CCCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVMFNDELFIRHGRGIQPTQRARQLFAPMRQALQLVKNELPSSTFIPETSMRQFKLSICS
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECC
PSDIRFAPRIMRDIQQKSPHISLKLSGEHEADIAQKLRYQEIDFVIDYVRFDEPGFMSTE
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCHH
LFTDELVVVAAVNHPRIQGMITKEQLANEKHAVPARIENTKGFADHLYQDAIYEQAYKGA
HHHCCEEEEEECCCCHHEEEEEHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCC
SISNILYVVSQSELITVAPKWLVSSMPNRDQLAILPLPIESKGISGYLSWHDSYQKDKGH
CHHHEEEEEECCCEEEECHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCC
IWMRDQLMLICGEEVVASSKGMMF
EEEECCEEEEECHHHHHCCCCCCC
>Mature Secondary Structure 
SMSEFSSASNSVRAEQVLRGVDLNLLTVFDAVMQEQNITRAAQNLGMSQPAVSNAVSRL
CCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVMFNDELFIRHGRGIQPTQRARQLFAPMRQALQLVKNELPSSTFIPETSMRQFKLSICS
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEECC
PSDIRFAPRIMRDIQQKSPHISLKLSGEHEADIAQKLRYQEIDFVIDYVRFDEPGFMSTE
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCHH
LFTDELVVVAAVNHPRIQGMITKEQLANEKHAVPARIENTKGFADHLYQDAIYEQAYKGA
HHHCCEEEEEECCCCHHEEEEEHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCC
SISNILYVVSQSELITVAPKWLVSSMPNRDQLAILPLPIESKGISGYLSWHDSYQKDKGH
CHHHEEEEEECCCEEEECHHHHHHCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCC
IWMRDQLMLICGEEVVASSKGMMF
EEEECCEEEEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]