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The map label for this gene is metB [H]

Identifier: 209696153

GI number: 209696153

Start: 2973481

End: 2974647

Strand: Direct

Name: metB [H]

Synonym: VSAL_I2747

Alternate gene names: 209696153

Gene position: 2973481-2974647 (Clockwise)

Preceding gene: 209696151

Following gene: 209696154

Centisome position: 89.42

GC content: 43.87

Gene sequence:

>1167_bases
ATGAGCGAGAGAAAACCTGCAACTATTGCTGTTCGTACTGGCATTGAAACCGATTCACAACACAATGCGGTTGTGCCTCC
TATCTATTTATCGACTAATTATAGCTTTCCAGAATTTGGAGATGTTCCAACGTACGATTACGCTCGCTCAGGGAATCCAA
CCCGAACTCAATTAGAAACAACATTGTCTGATTTAGAGTGTGGTGCGGGGGCGGTGGTGACGAACTGTGGCACTGCGGCT
CTTAATTTAATGGTATCAGCCTTGCTTGGTCCTGATGATTTAATTGTTGCCCCTCATGACTGCTATGGCGGCACTTATCG
ACTCTTTAACACCAGAGCAAATAAAGGTGATTTTCAAACGTTGTTTGTGGATCAGTCTAATGCCGCCGCTTTAGATGAAG
CGCTTGCTAAAAAGCCAAAACTGATCTTATTAGAAACGCCTTCCAATCCATTAGTTCGCGTAGTCGATATTGCCGAAATT
TGTAAAAAAGCGGCAGAAGTCGGTGCATTAGTTGCGGTTGATAACACGTTCCTTTCTCCTGTATTACAGCAGCCACTGAC
TCTTGGGGCTGATTTCGTTCTTCATTCCACAACAAAATACATTAATGGGCATTCAGACGTTGTTGGTGGTGTGATCATAT
CTAAAACGAAAGAACATGCCGATGAATTGGCATGGTGGGGGAACTGCATTGGTGCAACAGGTACTGCATTTGATAGCTAT
TTAACCCTGCGAGGAATTCGTACTCTTAGTGCTCGTATGCGAGTGCATGAAGAAAATGCGATGGAAGTATTAAAATGCTT
ACAACAGCAAGAGTTAGTGGGAACGATCTATCACCCTAGTCTACCTGAGCACCCAGGGCATGAAATAGCAAAGCGTCAGC
AAGCGGGTTTTGGTGCAATGCTGAGTTTTGAATTAAACGGTTCGTTAGACCAACTTAAAGTTTTTGTGAAAGCGTTAAAA
TATTTCTCTTTAGCTGAATCGTTAGGAGGAGTAGAAAGTCTGATTGCTCATCCGGGAACGATGACACATAGAGCGATGTC
AGATGAAGCTCAGGCTGCTGCCGGTTTGGTGCCGACGTTATTACGTATTTCTGTTGGCCTAGAAGACAGTAGAGATTTAA
TCGCAGATCTTACACAAGCGTTTGCTGTGGCTGCAGAGGTGAAATAA

Upstream 100 bases:

>100_bases
ACGTCTAGAAGTATTGACGTCTCATTTTGTGATGGTAAAGTAGAAGAATATAACTTATGGATTAGATTATACCACTGCAT
AGCAGTTTGGAAGGGAAATA

Downstream 100 bases:

>100_bases
TGACAACGTCTTTGGTGCAACGTCAGTTACATAAATTTGGTGGCAGCAGTCTTTCTGATCCTGAGTGTTATCGCCGTGTG
GCAACCATCTTAAAAGAATA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPTYDYARSGNPTRTQLETTLSDLECGAGAVVTNCGTAA
LNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNAAALDEALAKKPKLILLETPSNPLVRVVDIAEI
CKKAAEVGALVAVDNTFLSPVLQQPLTLGADFVLHSTTKYINGHSDVVGGVIISKTKEHADELAWWGNCIGATGTAFDSY
LTLRGIRTLSARMRVHEENAMEVLKCLQQQELVGTIYHPSLPEHPGHEIAKRQQAGFGAMLSFELNGSLDQLKVFVKALK
YFSLAESLGGVESLIAHPGTMTHRAMSDEAQAAAGLVPTLLRISVGLEDSRDLIADLTQAFAVAAEVK

Sequences:

>Translated_388_residues
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPTYDYARSGNPTRTQLETTLSDLECGAGAVVTNCGTAA
LNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNAAALDEALAKKPKLILLETPSNPLVRVVDIAEI
CKKAAEVGALVAVDNTFLSPVLQQPLTLGADFVLHSTTKYINGHSDVVGGVIISKTKEHADELAWWGNCIGATGTAFDSY
LTLRGIRTLSARMRVHEENAMEVLKCLQQQELVGTIYHPSLPEHPGHEIAKRQQAGFGAMLSFELNGSLDQLKVFVKALK
YFSLAESLGGVESLIAHPGTMTHRAMSDEAQAAAGLVPTLLRISVGLEDSRDLIADLTQAFAVAAEVK
>Mature_387_residues
SERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPTYDYARSGNPTRTQLETTLSDLECGAGAVVTNCGTAAL
NLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNAAALDEALAKKPKLILLETPSNPLVRVVDIAEIC
KKAAEVGALVAVDNTFLSPVLQQPLTLGADFVLHSTTKYINGHSDVVGGVIISKTKEHADELAWWGNCIGATGTAFDSYL
TLRGIRTLSARMRVHEENAMEVLKCLQQQELVGTIYHPSLPEHPGHEIAKRQQAGFGAMLSFELNGSLDQLKVFVKALKY
FSLAESLGGVESLIAHPGTMTHRAMSDEAQAAAGLVPTLLRISVGLEDSRDLIADLTQAFAVAAEVK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=382, Percent_Identity=40.5759162303665, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI299473759, Length=382, Percent_Identity=38.2198952879581, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI25453487, Length=379, Percent_Identity=36.1477572559367, Blast_Score=201, Evalue=7e-52,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=60.8923884514436, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789383, Length=350, Percent_Identity=31.1428571428571, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=43.1937172774869, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=42.5196850393701, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=33.2439678284182, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=389, Percent_Identity=38.3033419023136, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6321254, Length=415, Percent_Identity=34.9397590361446, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=32.1678321678322, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=31.7258883248731, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=27.639751552795, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322970, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922554, Length=383, Percent_Identity=40.4699738903394, Blast_Score=280, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41695; Mature: 41564

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPTYDYARSGNPTRTQLET
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TLSDLECGAGAVVTNCGTAALNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQT
HHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCCCCEEE
LFVDQSNAAALDEALAKKPKLILLETPSNPLVRVVDIAEICKKAAEVGALVAVDNTFLSP
EEEECCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEECHHHHHH
VLQQPLTLGADFVLHSTTKYINGHSDVVGGVIISKTKEHADELAWWGNCIGATGTAFDSY
HHHCHHHCCCHHEEECHHHHCCCCCHHHCCCEECCCHHHHHHHHHHHCCCCCCCHHHHHH
LTLRGIRTLSARMRVHEENAMEVLKCLQQQELVGTIYHPSLPEHPGHEIAKRQQAGFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELNGSLDQLKVFVKALKYFSLAESLGGVESLIAHPGTMTHRAMSDEAQAAAGLVPTL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCHHHHHHHHHHHHH
LRISVGLEDSRDLIADLTQAFAVAAEVK
HHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPTYDYARSGNPTRTQLET
CCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TLSDLECGAGAVVTNCGTAALNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQT
HHHHHCCCCCCEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCCCCEEE
LFVDQSNAAALDEALAKKPKLILLETPSNPLVRVVDIAEICKKAAEVGALVAVDNTFLSP
EEEECCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCEEEEECHHHHHH
VLQQPLTLGADFVLHSTTKYINGHSDVVGGVIISKTKEHADELAWWGNCIGATGTAFDSY
HHHCHHHCCCHHEEECHHHHCCCCCHHHCCCEECCCHHHHHHHHHHHCCCCCCCHHHHHH
LTLRGIRTLSARMRVHEENAMEVLKCLQQQELVGTIYHPSLPEHPGHEIAKRQQAGFGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELNGSLDQLKVFVKALKYFSLAESLGGVESLIAHPGTMTHRAMSDEAQAAAGLVPTL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHCHHHHHHHHHHHHH
LRISVGLEDSRDLIADLTQAFAVAAEVK
HHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]