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The map label for this gene is maeB [H]

Identifier: 209696151

GI number: 209696151

Start: 2971385

End: 2972632

Strand: Direct

Name: maeB [H]

Synonym: VSAL_I2745

Alternate gene names: 209696151

Gene position: 2971385-2972632 (Clockwise)

Preceding gene: 209696150

Following gene: 209696153

Centisome position: 89.36

GC content: 42.39

Gene sequence:

>1248_bases
ATGTCTGAAGATTTTCGCGAACAAGCTCTCCATTATCACGCTTACCCAACGCCGGGTAAAATTGAAATAGCACTGACTAA
ACCCGCCGATACGGTAGAAGACCTTGCTTTGGCTTATAGCCCAGGAGTAGCTGAACCAGTACGTGAAATCGCGAAAAACC
CAGACGATGTTTATAAATACACGTCTAAAGGCAATATGGTTGCGGTTATTTCAAATGGTACGGCTATTTTAGGCTTAGGT
AATTTAGGTCCTCTGGCTTCAAAACCTGTAATGGAAGGCAAATCATTACTTTTCAAACGATTTGCCGGTCTAGATTCGAT
TGATATCGAAGTGAAACATCGCACTATTGATGAATTTGTAGATACAGTGGCGAACATTGCAGATACTTTTGGTGGTATTA
ATTTAGAAGACATCAAAGCCCCTGATTGTTTTGAAATTGAACGTCGTTTAATTGAACGTTGTGATATTCCTGTATTCCAC
GATGATCAGCACGGTACTGCAATTGTAACGGCAGCGGGCATGTTGAATGCTATCGAGCTTCAAGGTAAGAAGTTAGAAGA
CGCGATCATTGTTTGTTTAGGTGCGGGTGCCGCGGCGGTTGCCTGCATGGAAATGCTGATTAAATGTGGCGCGATGCGCG
AGAAGATCTACATGCTTGATCGTAAAGGCGTAATTCACACTCGTCGTGATGACATTAATGAATATAAACAACGTTTTGCG
AACAACACAGATAAACGTACGCTTGAAGATGTTATTGAAGGCGCTGATTTATTCTTAGGGGTATCGGGTCCTAACCTATT
GCCACCTGAAGCGCTTAAATTAATGGCTGATAAGCCCGTTGTATTTGCTTGTTCAAATCCAGATCCTGAGATCAAACCAG
AATTGGCTCATGAAGTTCGTAATGATTTAATTATGGGTACTGGTCGTTCTGATTATCCTAACCAAGTAAATAACGTACTT
TGTTTCCCATTTATCTTCCGTGGTGCACTCGATGTTCGTGCAAGCGTGATTAATGATGAAATGAAATTGGCTGCGGTTGA
AGCAATTCGTCAATTAACGAAAGAACCTGTGCCTGTCGAAGTATTAAAAGCGGCGGGTGTTGATTCTTTAGAGTTTGGCA
AAGGTTACATCATTCCTAAACCGATGGATCCTCGTTTACTTCCTCGAGTTGCAAAAGCGGTAGCACAAGCCGCGGTTGAT
TCAGGTGTCGCTCGTATTGAAATGCCAGAAGGATACATGGAAGCGTAA

Upstream 100 bases:

>100_bases
TCTGTAAGCGAAGACTATGAAGTTTGAGCTAGATCAACTATTATATAGTTTACTATTTTGAAACAATTCTCTTATATTTA
TTAGCATTCAGGAAAACCCC

Downstream 100 bases:

>100_bases
ACTGCTTTTATATATTAATTAAAGTAAAAAGGCTTCCTACGTCATTGATGAGGAAGCCTTTTTATTAGAGGGAATTAAAT
TATGGGTTTACCACCAAACG

Product: NADP-dependent malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSEDFREQALHYHAYPTPGKIEIALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLG
NLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIERCDIPVFH
DDQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMEMLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
NNTDKRTLEDVIEGADLFLGVSGPNLLPPEALKLMADKPVVFACSNPDPEIKPELAHEVRNDLIMGTGRSDYPNQVNNVL
CFPFIFRGALDVRASVINDEMKLAAVEAIRQLTKEPVPVEVLKAAGVDSLEFGKGYIIPKPMDPRLLPRVAKAVAQAAVD
SGVARIEMPEGYMEA

Sequences:

>Translated_415_residues
MSEDFREQALHYHAYPTPGKIEIALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLG
NLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIERCDIPVFH
DDQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMEMLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
NNTDKRTLEDVIEGADLFLGVSGPNLLPPEALKLMADKPVVFACSNPDPEIKPELAHEVRNDLIMGTGRSDYPNQVNNVL
CFPFIFRGALDVRASVINDEMKLAAVEAIRQLTKEPVPVEVLKAAGVDSLEFGKGYIIPKPMDPRLLPRVAKAVAQAAVD
SGVARIEMPEGYMEA
>Mature_414_residues
SEDFREQALHYHAYPTPGKIEIALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLGN
LGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIERCDIPVFHD
DQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMEMLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFAN
NTDKRTLEDVIEGADLFLGVSGPNLLPPEALKLMADKPVVFACSNPDPEIKPELAHEVRNDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASVINDEMKLAAVEAIRQLTKEPVPVEVLKAAGVDSLEFGKGYIIPKPMDPRLLPRVAKAVAQAAVDS
GVARIEMPEGYMEA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=357, Percent_Identity=26.0504201680672, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI62420882, Length=351, Percent_Identity=28.7749287749288, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62420880, Length=351, Percent_Identity=28.7749287749288, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI239049447, Length=351, Percent_Identity=28.7749287749288, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4505145, Length=351, Percent_Identity=27.9202279202279, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI270265879, Length=317, Percent_Identity=27.7602523659306, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=58.3743842364532, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI87081919, Length=515, Percent_Identity=24.4660194174757, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537199, Length=414, Percent_Identity=25.8454106280193, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322823, Length=308, Percent_Identity=25.974025974026, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707242, Length=342, Percent_Identity=26.9005847953216, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI78707238, Length=342, Percent_Identity=26.9005847953216, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281362674, Length=456, Percent_Identity=25.219298245614, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI78707240, Length=342, Percent_Identity=26.9005847953216, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281362672, Length=456, Percent_Identity=25.219298245614, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356279, Length=456, Percent_Identity=25.219298245614, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24646388, Length=349, Percent_Identity=27.5071633237822, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646386, Length=349, Percent_Identity=27.5071633237822, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78707232, Length=351, Percent_Identity=23.3618233618234, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363505, Length=366, Percent_Identity=24.8633879781421, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI78707236, Length=366, Percent_Identity=24.8633879781421, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281363503, Length=366, Percent_Identity=23.224043715847, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 45329; Mature: 45198

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDFREQALHYHAYPTPGKIEIALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKY
CCHHHHHHHHEECCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHEE
TSKGNMVAVISNGTAILGLGNLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFV
CCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
DTVANIADTFGGINLEDIKAPDCFEIERRLIERCDIPVFHDDQHGTAIVTAAGMLNAIEL
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHH
QGKKLEDAIIVCLGAGAAAVACMEMLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHC
NNTDKRTLEDVIEGADLFLGVSGPNLLPPEALKLMADKPVVFACSNPDPEIKPELAHEVR
CCCCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
NDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASVINDEMKLAAVEAIRQLTKEPVPVE
HCEEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VLKAAGVDSLEFGKGYIIPKPMDPRLLPRVAKAVAQAAVDSGVARIEMPEGYMEA
HHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCC
>Mature Secondary Structure 
SEDFREQALHYHAYPTPGKIEIALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKY
CHHHHHHHHEECCCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHEE
TSKGNMVAVISNGTAILGLGNLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFV
CCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
DTVANIADTFGGINLEDIKAPDCFEIERRLIERCDIPVFHDDQHGTAIVTAAGMLNAIEL
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHH
QGKKLEDAIIVCLGAGAAAVACMEMLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHC
NNTDKRTLEDVIEGADLFLGVSGPNLLPPEALKLMADKPVVFACSNPDPEIKPELAHEVR
CCCCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
NDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASVINDEMKLAAVEAIRQLTKEPVPVE
HCEEECCCCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VLKAAGVDSLEFGKGYIIPKPMDPRLLPRVAKAVAQAAVDSGVARIEMPEGYMEA
HHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]