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The map label for this gene is pilD [H]

Identifier: 209696040

GI number: 209696040

Start: 2848634

End: 2849521

Strand: Direct

Name: pilD [H]

Synonym: VSAL_I2632

Alternate gene names: 209696040

Gene position: 2848634-2849521 (Clockwise)

Preceding gene: 209696039

Following gene: 209696041

Centisome position: 85.67

GC content: 39.86

Gene sequence:

>888_bases
ATGGCTGCTTTTGAATACTACCCTTGGCTCTTTCCTCTCTTTGCTACTTTATTTGGCCTACTCGTCGGTAGCTTTTTAAA
TGTGGTCATCTACCGTTTACCCATAATGATGGAAAAAGAATGGAAAAAAGAATGCACTGAGTGCTTTCCTGATTTGAAAT
CCACACCAAAAACAGCGGATATAGAAGAAAAATTCAATCTAAGTGTTCCTCGTTCACGCTGCCCTAAATGCAATACTCAA
ATAAAATTTTACGATAACATTCCCGTTATTAGCTGGTTGTTGTTGCGTGGAAAATGCCGTCAATGCAGTAATCCTATTAG
CTTCCGTTATCCTGCCATTGAGCTTCTGTCAGGCGCAATGTGTTTTGGCGTCTCTTACTTATTACCATTTTCTTATTTTG
CTATTGCTGCCGTTCTTTTTACTCTAACGCTTATTGCATTAACCTTTATTGATATCGATACCATGTTACTCCCCGATCAA
ATAACGCTGCCATTACTGTGGACTGGCCTATACTTATCCTTAGTCGGTTGGAGTTCAGTCAGCTTAATGGATTCGGTCAT
TGGAGCCATGGCTGGTTATTTAATATTATGGTCCATTTATTGGGCATTTAAATTATTAACCGGTAAAGAAGGCATGGGTT
ATGGGGACTTTAAATTATTAGCCGCACTTGGTGCTTGGCTTGGTTGGCAACAATTGCCATTAATCGTTTTATTGTCTTCT
ATTGTTGGTGCCATCATTGGCATTGCAATGTTAAGGGCACAAAAAAATGGGTATGACAAAGCCATTCCATTTGGTCCTTA
TCTCGCGATTGCAGGTTGGATATGTTTATTGTGGGGCCAAGAAATTAGCCAATGGTACTTCACCTCAATTCTAGGGTTTC
CCGTATGA

Upstream 100 bases:

>100_bases
TAATTCAACCATGTTACCGCGACTCTCTCCTACTTCATTGCACCGAACTCTGATAAAATTAATTAACTAAAAATATAATT
AATATCGGATGCAATTTCAC

Downstream 100 bases:

>100_bases
GTTATGTCATCGCTATTACGGGTGGTATAGGCAGTGGTAAAACGACAATTGCAGATAAATTTAACCAATTATATGACATT
GATATTGTTGATGCCGATAT

Product: type IV prepilin peptidase PilD (type IV-A prepilin peptidase PilD) (leader peptidase PilD)

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAAFEYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKECTECFPDLKSTPKTADIEEKFNLSVPRSRCPKCNTQ
IKFYDNIPVISWLLLRGKCRQCSNPISFRYPAIELLSGAMCFGVSYLLPFSYFAIAAVLFTLTLIALTFIDIDTMLLPDQ
ITLPLLWTGLYLSLVGWSSVSLMDSVIGAMAGYLILWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIVLLSS
IVGAIIGIAMLRAQKNGYDKAIPFGPYLAIAGWICLLWGQEISQWYFTSILGFPV

Sequences:

>Translated_295_residues
MAAFEYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKECTECFPDLKSTPKTADIEEKFNLSVPRSRCPKCNTQ
IKFYDNIPVISWLLLRGKCRQCSNPISFRYPAIELLSGAMCFGVSYLLPFSYFAIAAVLFTLTLIALTFIDIDTMLLPDQ
ITLPLLWTGLYLSLVGWSSVSLMDSVIGAMAGYLILWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIVLLSS
IVGAIIGIAMLRAQKNGYDKAIPFGPYLAIAGWICLLWGQEISQWYFTSILGFPV
>Mature_294_residues
AAFEYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKECTECFPDLKSTPKTADIEEKFNLSVPRSRCPKCNTQI
KFYDNIPVISWLLLRGKCRQCSNPISFRYPAIELLSGAMCFGVSYLLPFSYFAIAAVLFTLTLIALTFIDIDTMLLPDQI
TLPLLWTGLYLSLVGWSSVSLMDSVIGAMAGYLILWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIVLLSSI
VGAIIGIAMLRAQKNGYDKAIPFGPYLAIAGWICLLWGQEISQWYFTSILGFPV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=269, Percent_Identity=38.2899628252788, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789732, Length=149, Percent_Identity=45.6375838926174, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33226; Mature: 33095

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAFEYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKECTECFPDLKSTPKTAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IEEKFNLSVPRSRCPKCNTQIKFYDNIPVISWLLLRGKCRQCSNPISFRYPAIELLSGAM
CHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CFGVSYLLPFSYFAIAAVLFTLTLIALTFIDIDTMLLPDQITLPLLWTGLYLSLVGWSSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
SLMDSVIGAMAGYLILWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIVLLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHH
IVGAIIGIAMLRAQKNGYDKAIPFGPYLAIAGWICLLWGQEISQWYFTSILGFPV
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339 [H]