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The map label for this gene is pilB [H]

Identifier: 209696039

GI number: 209696039

Start: 2845569

End: 2847260

Strand: Direct

Name: pilB [H]

Synonym: VSAL_I2630

Alternate gene names: 209696039

Gene position: 2845569-2847260 (Clockwise)

Preceding gene: 209696038

Following gene: 209696040

Centisome position: 85.58

GC content: 42.26

Gene sequence:

>1692_bases
ATGCACACCAACCTAGCTTCAATCTTGCGTCAAGCTGAACTCATCAGCTTGACGCAAGAAAATACCATTATCGAACACGT
TCAATCAGGGGCACACACTGTGCCTTCTGCGCTTTTAGCTGCTCATATTTTTACACCTGATGAATTAATCAGTCATCTTT
CTCAAATTTTTGGTTTGGATGTCATTAATGTCGCTCAATATGATTACGCCACGTGCTGTCAAAAATTAGGACTAAGAGAG
CTCATAACCCAACATCAAGCATTGCCGATTCATTATGATCACGGCTTGATTCGACTCGCAGTGAGTGATCCAAGTATTGC
TCAAATTGAGGATGATTTTCGTTTCACTACCAACAAACAAATTGAATTAGTGTTAGCTGACGAAGGACAGCTTCAAGCGG
CCATTCGGCGTCTTTATGGGAAAGGGTTATCGGATTCACTGCCATCACAAAATAAAGAAATCACCCAAGATGAGCTCGCA
TCACTGGTTGATATATCAAATGATGAAATGGTATTCACAGAAGATCTCAGCCAAGACGACGCCCCGGTTACCCGTTTTAT
AAACCAAATATTACTAGAGGCCGTTCGAAAAGGCGTTTCTGATATTCACTTTGAACCTTATGAAGAAACTTATCGTGTGC
GTTTTCGTTGTGATGGGATTTTAGTAGAGAACAGTAGTCCTTCTTCTCATTTAAGTCGTCATCTTTCAGCCCGCTTAAAA
ATCATGGCAAAACTTGATATCGCAGAGCGACGATTACCACAAGACGGACGCATCAAACTTAAACTGAACAGCGAAGTCTC
CATTGATATGCGCGTGTCCTCTCTTCCTACGTTATGGGGAGAGAAGATTGTTCTTCGATTATTAGACAGCAGCGCGGCCA
GTTTGGATATCGACAAACTCGGCTACAGTGACGCCCAAAAAGAGTTATACCTAACGGCATTAAAACGCCCTCAAGGCATG
ATCTTAATGACGGGCCCGACCGGCAGTGGCAAAACCGTATCTCTTTACTCTGGCCTAAACATTTTAAATACGACAGAAAA
AAACATCTCCACCGCAGAAGATCCTGTCGAAATAAATCTGAATGGCATTAACCAAGTTCAAGTAAACCCTAAAATTGGAT
TTACCTTTGCTTACGCCTTGCGATCATTTCTTCGTCAAGATCCCGATATTGTAATGGTCGGAGAGATCCGAGATCTAGAA
ACAGCCGAAATCGCAGTAAAAGCCGCACAAACTGGGCACTTAGTTCTTTCAACACTTCATACCAACTCAGCGGCAGAAAC
AGTGGTACGTTTAGGAAATATGGGGATCGAGAATTTTAACCTTGCCTCCTCTCTTACTCTTATCATTGCTCAACGATTAG
CAAGAAGGCTTTGCCCTCATTGCAAAGCTATCGATCCATTAACCCCAGAGATCAGAGAAGAATATGGTATTGATTTATTA
GGCACTGTTTTTCAAGCGCACACTGAAGGCTGTAATGAATGTACGCATGGCTATTCAGGGCGAATTGGTATTTACGAAGT
GATGCCCTTTACCCGAGAGCTTTCCATTGCGATTTTAAAAGGCGCAACGGTAAGACAGATTGAACAACTTGCTATCAAGC
AAGGCATGGATAATTTAGCCACATCAGGAATTAAACGCCTCGAAGATGGTACAACCAGCTTACAAGAATTAACTCGTGTT
CTGTATTTTTAA

Upstream 100 bases:

>100_bases
AAACATTAACACTAGCAAGAAATTCTGACACTGGTTGGTCTTGTACTCCATCTACAGGTGTTCCTGTTATGGATGGTTGC
AAAATAGTAACACCATAATA

Downstream 100 bases:

>100_bases
TCACTCAGCAGTAAAGTGAATTTCCCCATGCTAAAAAAAACAACAAAAGTAAAAACACCGACCTTAAAAAACTTCCATTG
GCGTGGTATCAATAGCACAG

Product: type IV pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLDVINVAQYDYATCCQKLGLRE
LITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQIELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELA
SLVDISNDEMVFTEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKLGYSDAQKELYLTALKRPQGM
ILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINLNGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLATSGIKRLEDGTTSLQELTRV
LYF

Sequences:

>Translated_563_residues
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLDVINVAQYDYATCCQKLGLRE
LITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQIELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELA
SLVDISNDEMVFTEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKLGYSDAQKELYLTALKRPQGM
ILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINLNGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLATSGIKRLEDGTTSLQELTRV
LYF
>Mature_563_residues
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLDVINVAQYDYATCCQKLGLRE
LITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQIELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELA
SLVDISNDEMVFTEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKLGYSDAQKELYLTALKRPQGM
ILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINLNGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLATSGIKRLEDGTTSLQELTRV
LYF

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=416, Percent_Identity=47.8365384615385, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1786296, Length=490, Percent_Identity=41.4285714285714, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=32.6241134751773, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62438; Mature: 62438

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VINVAQYDYATCCQKLGLRELITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQ
HHHHHHCCHHHHHHHCCHHHHHHHCCCCEEEECCCEEEEEECCCCCEEECCCCEECCCCE
IELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELASLVDISNDEMVFTEDLSQDD
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCC
APVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
CHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKL
HHHHCCHHHHHCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHC
GYSDAQKELYLTALKRPQGMILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINL
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEE
NGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEECCCCCEEEEEEC
TNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHH
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLA
HHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHEEEEECCCHHHHHHHHHHHHCHHHHH
TSGIKRLEDGTTSLQELTRVLYF
HHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VINVAQYDYATCCQKLGLRELITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQ
HHHHHHCCHHHHHHHCCHHHHHHHCCCCEEEECCCEEEEEECCCCCEEECCCCEECCCCE
IELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELASLVDISNDEMVFTEDLSQDD
EEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCC
APVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
CHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHH
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKL
HHHHCCHHHHHCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHC
GYSDAQKELYLTALKRPQGMILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINL
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEE
NGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLETAEIAVKAAQTGHLVLSTLH
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEEEECCCCCEEEEEEC
TNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHH
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLA
HHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHEEEEECCCHHHHHHHHHHHHCHHHHH
TSGIKRLEDGTTSLQELTRVLYF
HHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]