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The map label for this gene is folD

Identifier: 209695646

GI number: 209695646

Start: 2376454

End: 2377311

Strand: Reverse

Name: folD

Synonym: VSAL_I2209

Alternate gene names: 209695646

Gene position: 2377311-2376454 (Counterclockwise)

Preceding gene: 209695648

Following gene: 209695645

Centisome position: 71.49

GC content: 44.17

Gene sequence:

>858_bases
ATGACCGCTCAAATTATCGATGGAAAACTTATTTCTCAAACTGTTCGCTCTGAAGTCGGTGCTCGTGTAAAAGCCCGCAT
AGAGGCCGGACTGCGTGCTCCTGGTCTAGCCGTTGTTTTGGTAGGACAAGATCCTGCGTCTCAAGTCTATGTAGGAAGTA
AACGCCGTGCCTGTGAAGAAGTTGGATTCGTATCAAAATCTTATGATTTACCAACCACGACCACAGAAGCGGAATTGCTG
ACATTAATTGATACACTAAATCAAGATCCTGAAATCGATGGTATTTTGGTTCAGTTACCTCTTCCTGCAGGAATGGATAG
CACTAAAATTTTAGAGCATATCGATCCAGAAAAAGACGTCGATGGATTTCACCCGTACAACGTAGGACGTCTATCTCAAC
GTATTCCAAAATTACGTTCATGCACACCAAAAGGCATTATCACTCTACTTGATCGCTACAATATTCAAGTTCGAGGGATG
CACGCTGTTGTCGTTGGGGCCTCAAATATTGTTGGTCGCCCGATGACCCTTGAATTGCTTCTTGCTGGTTGCACCACGAC
GACTTGTCACCGTTTCACAAAAGATTTAGAGCATCACATTCGCCAAGCTGATCTTGTTGTGGTTGCGGTAGGCAAACCAA
ACTTTATCCCTGGAGAATGGATAAAAGAAGGCGCTGTTGTGGTCGATGTTGGTATTAACCGTCTAGACACAGGTAAGCTC
ATTGGTGACGTTGAATTTGATGTAGCCAAAACAAAAGCAAGCTATATCACTCCCGTTCCTGGTGGCGTTGGTCCAATGAC
CGTAGCAAGTTTAATTGAAAACACATTATTGGCTTGCGAGCAATTCCACAGCAAATAG

Upstream 100 bases:

>100_bases
TTCAAAAACAGCAAAAAGCAAACGTTTGCGTTGTAGCTATTATTATTTAATTCTGAGACAATGCGCACGTCAATCAGTCC
AAGCCCTTGAAAGGATCACT

Downstream 100 bases:

>100_bases
TCATCAAGCCTATTAATCAAAATGTCAATTAGCGGACTTTATTAAGTTCGCTTTTTTTATACCTGCGTGTTGCCGCATTT
CTCGATCAATTTTTATTCTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAQIIDGKLISQTVRSEVGARVKARIEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTTEAELL
TLIDTLNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGM
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEHHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDTGKL
IGDVEFDVAKTKASYITPVPGGVGPMTVASLIENTLLACEQFHSK

Sequences:

>Translated_285_residues
MTAQIIDGKLISQTVRSEVGARVKARIEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTTEAELL
TLIDTLNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGM
HAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEHHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDTGKL
IGDVEFDVAKTKASYITPVPGGVGPMTVASLIENTLLACEQFHSK
>Mature_284_residues
TAQIIDGKLISQTVRSEVGARVKARIEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEEVGFVSKSYDLPTTTTEAELLT
LIDTLNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDVDGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMH
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKDLEHHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDTGKLI
GDVEFDVAKTKASYITPVPGGVGPMTVASLIENTLLACEQFHSK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=299, Percent_Identity=42.1404682274247, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=43.3898305084746, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=43.1972789115646, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=29.2929292929293, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=74.5583038869258, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=41.1764705882353, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=43.4782608695652, Blast_Score=236, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=42.9065743944637, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=289, Percent_Identity=28.0276816608997, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=47.8260869565217, Blast_Score=251, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=47.8260869565217, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=41.7241379310345, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=41.7241379310345, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=41.7241379310345, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=41.7241379310345, Blast_Score=211, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ALISL (B6EIM4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263575.1
- GeneID:   6989008
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2209
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK10792
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30779; Mature: 30648

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIIDGKLISQTVRSEVGARVKARIEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEE
CCCCCCCCHHHHHHHHHHHCCHHEEHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPTTTTEAELLTLIDTLNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCEEEEEEEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEHHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDTGKL
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECCCEECCCCE
IGDVEFDVAKTKASYITPVPGGVGPMTVASLIENTLLACEQFHSK
EECEEEHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQIIDGKLISQTVRSEVGARVKARIEAGLRAPGLAVVLVGQDPASQVYVGSKRRACEE
CCCCCCCHHHHHHHHHHHCCHHEEHHHCCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFVSKSYDLPTTTTEAELLTLIDTLNQDPEIDGILVQLPLPAGMDSTKILEHIDPEKDV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLSQRIPKLRSCTPKGIITLLDRYNIQVRGMHAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCHHHHCCCCHHHHHHHHHCCEEEEEEEEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKDLEHHIRQADLVVVAVGKPNFIPGEWIKEGAVVVDVGINRLDTGKL
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEECCCEECCCCE
IGDVEFDVAKTKASYITPVPGGVGPMTVASLIENTLLACEQFHSK
EECEEEHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA