Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 209695641

GI number: 209695641

Start: 2368327

End: 2368959

Strand: Reverse

Name: rluE [H]

Synonym: VSAL_I2204

Alternate gene names: 209695641

Gene position: 2368959-2368327 (Counterclockwise)

Preceding gene: 209695643

Following gene: 209695635

Centisome position: 71.24

GC content: 41.86

Gene sequence:

>633_bases
ATGACATTCAAGCGTTCTCCAAATTGGAAAAAACCGGCCTTTAAGAAAAAAACCACACAAAAGCCACGCCCTGCACCAGA
AGATCGTAAAGTCCTTATCTTTAATAAACCTTTTGATGTATTGAGTCAATTTACCGATGATCAGCATCGACAAACTCTTG
CAGATTTCATTTCTGTGAAGGATGTTTACGCAGCAGGACGCCTTGATCGTGACAGTGAAGGATTATTAATTCTTACCAAT
GATGGTGTGCTTCAAGCACGGTTAACACAACCTGAATCTAAGTCACCAAAAACCTATTGGGTACAAGTTGAAGGTGCTCC
GACAGAAGACGATTTAGAGAAGCTTCGCCAAGGCGTTGAATTAAAAGATGGGATGACACTTCCCGCAAAAGTTGAAGTAA
TGACAGAGCCTGAACTATGGGAAAGAAACCCTCCCGTTCGCTTTAGAGCAGCGATTCCGACAACATGGATAGCAGTGACT
ATTATTGAAGGAAGAAACAGACAAGTTCGCCGAATGACGGCTCACATTGGGTTTCCAACGTTACGCTTGATTCGTTACTC
TATGGGAGATTGGACGATCAAAGATGTCCCTCATCGAGAATGGAAAGAAGTGACAATTAATAGTAATAAATAG

Upstream 100 bases:

>100_bases
TTTTTATTAGTCTTTTGTTTCACGCGCGTACTATAGCGAAAAGCCGTGAACTTTAAAACTAAACACCACAAAAGCTTTAC
AATTTTGCAAGGTAGTTAAC

Downstream 100 bases:

>100_bases
AAATGTAGCAAATAAGTTAAAAACAACTACATATTTACGTACTTTTCACTTAAACTAAATAGATAATCACATGAGAATAA
AGGAATAAAAATGAAGATAA

Product: ribosomal large subunit pseudouridine synthase E

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTFKRSPNWKKPAFKKKTTQKPRPAPEDRKVLIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTN
DGVLQARLTQPESKSPKTYWVQVEGAPTEDDLEKLRQGVELKDGMTLPAKVEVMTEPELWERNPPVRFRAAIPTTWIAVT
IIEGRNRQVRRMTAHIGFPTLRLIRYSMGDWTIKDVPHREWKEVTINSNK

Sequences:

>Translated_210_residues
MTFKRSPNWKKPAFKKKTTQKPRPAPEDRKVLIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTN
DGVLQARLTQPESKSPKTYWVQVEGAPTEDDLEKLRQGVELKDGMTLPAKVEVMTEPELWERNPPVRFRAAIPTTWIAVT
IIEGRNRQVRRMTAHIGFPTLRLIRYSMGDWTIKDVPHREWKEVTINSNK
>Mature_209_residues
TFKRSPNWKKPAFKKKTTQKPRPAPEDRKVLIFNKPFDVLSQFTDDQHRQTLADFISVKDVYAAGRLDRDSEGLLILTND
GVLQARLTQPESKSPKTYWVQVEGAPTEDDLEKLRQGVELKDGMTLPAKVEVMTEPELWERNPPVRFRAAIPTTWIAVTI
IEGRNRQVRRMTAHIGFPTLRLIRYSMGDWTIKDVPHREWKEVTINSNK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=60.6217616580311, Blast_Score=251, Evalue=2e-68,
Organism=Escherichia coli, GI1790453, Length=186, Percent_Identity=30.6451612903226, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=32.967032967033, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=31.8681318681319, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 24338; Mature: 24207

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKRSPNWKKPAFKKKTTQKPRPAPEDRKVLIFNKPFDVLSQFTDDQHRQTLADFISVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHH
DVYAAGRLDRDSEGLLILTNDGVLQARLTQPESKSPKTYWVQVEGAPTEDDLEKLRQGVE
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
LKDGMTLPAKVEVMTEPELWERNPPVRFRAAIPTTWIAVTIIEGRNRQVRRMTAHIGFPT
CCCCCCCCEEEEEEECCHHHCCCCCEEEEEECCCEEEEEEEEECCCCEEHHHHHHCCCCH
LRLIRYSMGDWTIKDVPHREWKEVTINSNK
HHHEEHHCCCEEECCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TFKRSPNWKKPAFKKKTTQKPRPAPEDRKVLIFNKPFDVLSQFTDDQHRQTLADFISVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHH
DVYAAGRLDRDSEGLLILTNDGVLQARLTQPESKSPKTYWVQVEGAPTEDDLEKLRQGVE
HHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHCCCC
LKDGMTLPAKVEVMTEPELWERNPPVRFRAAIPTTWIAVTIIEGRNRQVRRMTAHIGFPT
CCCCCCCCEEEEEEECCHHHCCCCCEEEEEECCCEEEEEEEEECCCCEEHHHHHHCCCCH
LRLIRYSMGDWTIKDVPHREWKEVTINSNK
HHHEEHHCCCEEECCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA