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The map label for this gene is msbB [H]

Identifier: 209695614

GI number: 209695614

Start: 2336134

End: 2337075

Strand: Direct

Name: msbB [H]

Synonym: VSAL_I2175

Alternate gene names: 209695614

Gene position: 2336134-2337075 (Clockwise)

Preceding gene: 209695612

Following gene: 209695615

Centisome position: 70.26

GC content: 39.17

Gene sequence:

>942_bases
ATGAGCGAAAAAATCGATAAAAATGTATATAACCCAACTTTTCAATGGGCATTCCTTCATCCTAGATATTGGCCTACGTG
GTTAGCTGTTGGTTTTGGTATTATTTTAGCGTTTGTTCCTTTTCGATTGCGCGATAAACTTGCCGCTAAAATCGTTAAAT
TAGTTTTAAAGAAACCAAGCAGTGGTATTAAACGAGCGAAGTTGAATATTGCGACGTGTTTTCCTGAAAAATCTGAGCAA
GAAAAACAAGAATTGTTAGAAAAAACATACATTACGGGAGCTCAAGTTATGCTTGGTCTTTCTGAGCTGCTTGTTCGTAG
TCGTAAGCATAATGAAGCGCGTGGTATTTTTGTTGGTGGAGAAAATTTATTTCCTTTACTTGAACAAAAAGAGAATATTA
TTATTTTAGCCCCTCATGCTTGGGCTATCGATTTCCCTGCTATTTTACTTGCATCGCGTGGTTACCATGTGACTACGATG
ATGAAACCACAGAAAAACCCAATCACTGATTGGTTAATGCATGTTCAGCGTATGCAATATGGTGGGAAGATTTACGCACG
TAGCGCTGGAATAAAACCATTGATTAAAGCGATTAAAGAAGGGTACTTAGCGTATTACCTTCCAGATGAAGATCATGGGC
CACAAAACAGTGTTTTTGTTCCTTTTTTCGGTACGCAAAAAGCAACGCTTAAAGGCTTTGGTAAATTGGCGCGCTTGTCT
AAAGCGAAAGTGGTTCCAATGTTGCCATCATATAATGCAGAAACGGGTCAATTTGAAATTATTACCTTACCTGCGATTGC
CGATTTCCCAGCAGGTGATGAAGAAACAGACGCTCGGTCAATGAATATTGCGATCGAACAATTACTTGAGGGACATCCTG
AGCAATATATGTGGGTGCTTAATTTATTGCGTTCTCGACCAGATGGCAGTCGTCTCTATTAA

Upstream 100 bases:

>100_bases
CGTGGATGTAATTGAAAAGCCATTCCATTTCCTTGGTTTCTATCAGTAATGCTTTGTTATATCATAATCAGTTCATAATA
AAGAATAAGTGAAGCGATTC

Downstream 100 bases:

>100_bases
ATAATAGAGAATGAGGTCGATATCGTTTATAGCCCGTTTAGTGGAAACACTAAGCGGGCTTTTTATTGCTATAAATTGTT
AATAATAGTGCTCTTATTGA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: KDO2-lipid A, cold adapted; [acyl carrier protein]; KDO2-lipid A [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSEKIDKNVYNPTFQWAFLHPRYWPTWLAVGFGIILAFVPFRLRDKLAAKIVKLVLKKPSSGIKRAKLNIATCFPEKSEQ
EKQELLEKTYITGAQVMLGLSELLVRSRKHNEARGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTM
MKPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFGTQKATLKGFGKLARLS
KAKVVPMLPSYNAETGQFEIITLPAIADFPAGDEETDARSMNIAIEQLLEGHPEQYMWVLNLLRSRPDGSRLY

Sequences:

>Translated_313_residues
MSEKIDKNVYNPTFQWAFLHPRYWPTWLAVGFGIILAFVPFRLRDKLAAKIVKLVLKKPSSGIKRAKLNIATCFPEKSEQ
EKQELLEKTYITGAQVMLGLSELLVRSRKHNEARGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTM
MKPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFGTQKATLKGFGKLARLS
KAKVVPMLPSYNAETGQFEIITLPAIADFPAGDEETDARSMNIAIEQLLEGHPEQYMWVLNLLRSRPDGSRLY
>Mature_312_residues
SEKIDKNVYNPTFQWAFLHPRYWPTWLAVGFGIILAFVPFRLRDKLAAKIVKLVLKKPSSGIKRAKLNIATCFPEKSEQE
KQELLEKTYITGAQVMLGLSELLVRSRKHNEARGIFVGGENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMM
KPQKNPITDWLMHVQRMQYGGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFGTQKATLKGFGKLARLSK
AKVVPMLPSYNAETGQFEIITLPAIADFPAGDEETDARSMNIAIEQLLEGHPEQYMWVLNLLRSRPDGSRLY

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=38.0645161290323, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1787292, Length=310, Percent_Identity=32.9032258064516, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082094, Length=309, Percent_Identity=26.8608414239482, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35487; Mature: 35356

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKIDKNVYNPTFQWAFLHPRYWPTWLAVGFGIILAFVPFRLRDKLAAKIVKLVLKKPS
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGIKRAKLNIATCFPEKSEQEKQELLEKTYITGAQVMLGLSELLVRSRKHNEARGIFVGG
CCCHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECC
ENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMMKPQKNPITDWLMHVQRMQY
CCCCHHHCCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFGTQKATLKGFGKLARLS
CCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KAKVVPMLPSYNAETGQFEIITLPAIADFPAGDEETDARSMNIAIEQLLEGHPEQYMWVL
HCCEEECCCCCCCCCCCEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
NLLRSRPDGSRLY
HHHHCCCCCCCCC
>Mature Secondary Structure 
SEKIDKNVYNPTFQWAFLHPRYWPTWLAVGFGIILAFVPFRLRDKLAAKIVKLVLKKPS
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGIKRAKLNIATCFPEKSEQEKQELLEKTYITGAQVMLGLSELLVRSRKHNEARGIFVGG
CCCHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECC
ENLFPLLEQKENIIILAPHAWAIDFPAILLASRGYHVTTMMKPQKNPITDWLMHVQRMQY
CCCCHHHCCCCCEEEECCCCCHHCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
GGKIYARSAGIKPLIKAIKEGYLAYYLPDEDHGPQNSVFVPFFGTQKATLKGFGKLARLS
CCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KAKVVPMLPSYNAETGQFEIITLPAIADFPAGDEETDARSMNIAIEQLLEGHPEQYMWVL
HCCEEECCCCCCCCCCCEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
NLLRSRPDGSRLY
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-(palmitoleoyl)-lipid IVA; myristoyl-ACP; KDO2-(lauroyl)-lipid IVA [C]

Specific reaction: KDO2-(palmitoleoyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A, cold adapted + [acyl carrier protein] KDO2-(lauroyl)-lipid IVA + myristoyl-ACP = KDO2-lipid A + [acyl carrier protein] [C]

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]