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The map label for this gene is clcA [H]

Identifier: 209695615

GI number: 209695615

Start: 2337528

End: 2338928

Strand: Direct

Name: clcA [H]

Synonym: VSAL_I2176

Alternate gene names: 209695615

Gene position: 2337528-2338928 (Clockwise)

Preceding gene: 209695614

Following gene: 209695620

Centisome position: 70.3

GC content: 43.4

Gene sequence:

>1401_bases
ATGCCTAAATCACCCAATTTCCGCTTTTTTCAAGGCGGTCTGTTAAAACACCGTACCACACCGAAATTCTTAACAACTCA
AACTGAATATTTTTCATTGCTTTTTTTATCGGCACTCGTTGGGGGGATCACCGGCTCTGTAGTTGCATTATTCGAAGAGT
CTATTCGTTGGATCACAGAGCAGCGTTTAACGTTTGTTCATACGCTTCCTTACGCTGAATATATTGCTCTGGTTGCGGCT
GTTTTAGCCGGGGCGGCTATGGCTGCGGTTGGTTTTTGGTTAACGTTTAAATTTGCACCAGAAGTGGCGGGCAGTGGTAT
TCCCCATATTGAAGGGGCACTTGATGGTACGCATGAAATCCGGTGGCATAGAGTATTACCGATTAAGTTTATTGCTGGCA
CATTAACTATTGGATCCGGGATGGTACTGGGTCGAGAAGGGCCATCTATTCAAATTGGTGGTGCCGTTGGACGAATGATT
GCCAGTAAAGGTAAGCGTTTTAGTCACACAACTCATATTTTAACCGCAGCAGGTGCGGCGGCGGGGTTGGCTGCGGCATT
TAATGCTCCTCTTGCTGGTATCCTATTTGTTGTCGAAGAAATGCGTCCGCAGTTTCGTTACAGTATTACGTCGATTAAAT
GCGTTACATTAGCGACAGCAGTCGCCACTATTGTTATGCGTTCTTTGCATGGACAAGCCGCGTTCTTGGATATACCTCAC
TTTGATGTCCCTCCATTACGATCTCTTATCTTATTTGCTGTTTTGGGTATTTTATTTGGGGTGATTGGGGTTAACTTTAA
TAAGTGGATTTTGAAAGGGACAAGTCGAGTTAAAGAATATCATCAAAATAAATTATCACGATTAGTGCTTGTTGGTGCGA
TGTTTGGTGGTACTTTTGCTTTTTTACAATTGGTGGTTCCTAAACTGTCAGGCAGTGGGATGGAGATCATTACAGAAATG
TTTCATAGTCCAATGATGTGGATGGTAATGGTTGGATTTTTTGTTCTGAGAATTATTGTTACCGTCGCTTGTTTTGCATC
TGGTGCTCCGGGGGGGATTTTTGCCCCAATGCTAACTCTAGGTACCTTATTAGGTTTGTCTTATGGCATCGTGATGGCGG
CTTGGTTTCCTGAATGGGTTTCAGAACCCGGAGTTTACGCGATTGCAGGTATGGGGGCACTGTTTGCTGCCACAGTTCGA
GCTCCGGTAACGGGGATAATACTCGTTGTTGAAATGACAAATAATTATCAACTAATTTTACCATTATTAGTGACGTGCCT
AGGGGCTACCTTTATTGCGCAAGCTTTAGGCGGTCAACCGTTGTATTCAGCATTACTCAAACAATCGTTACTTGAGGATA
AAGAGTTGAGGGTTGATAAAAATAGCCCAGCAAGTAATTAA

Upstream 100 bases:

>100_bases
TTTTCTCAAAGCGTATACTTCTTATTACATTATACCTCTTTAGTTCCACGTGTTCTCTGTTCAAATAAAAATGCCGTTTA
GCGCTAAGTTGACCAAATAA

Downstream 100 bases:

>100_bases
AATAGATAACTCAGTATTAATTGACTCGAAATGAAGTCCACTGCAATACCATCTGCAACCATACGTCCCATCGTGTAACT
TGCTGCTATATTCATCGAAT

Product: chloride channel protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPKSPNFRFFQGGLLKHRTTPKFLTTQTEYFSLLFLSALVGGITGSVVALFEESIRWITEQRLTFVHTLPYAEYIALVAA
VLAGAAMAAVGFWLTFKFAPEVAGSGIPHIEGALDGTHEIRWHRVLPIKFIAGTLTIGSGMVLGREGPSIQIGGAVGRMI
ASKGKRFSHTTHILTAAGAAAGLAAAFNAPLAGILFVVEEMRPQFRYSITSIKCVTLATAVATIVMRSLHGQAAFLDIPH
FDVPPLRSLILFAVLGILFGVIGVNFNKWILKGTSRVKEYHQNKLSRLVLVGAMFGGTFAFLQLVVPKLSGSGMEIITEM
FHSPMMWMVMVGFFVLRIIVTVACFASGAPGGIFAPMLTLGTLLGLSYGIVMAAWFPEWVSEPGVYAIAGMGALFAATVR
APVTGIILVVEMTNNYQLILPLLVTCLGATFIAQALGGQPLYSALLKQSLLEDKELRVDKNSPASN

Sequences:

>Translated_466_residues
MPKSPNFRFFQGGLLKHRTTPKFLTTQTEYFSLLFLSALVGGITGSVVALFEESIRWITEQRLTFVHTLPYAEYIALVAA
VLAGAAMAAVGFWLTFKFAPEVAGSGIPHIEGALDGTHEIRWHRVLPIKFIAGTLTIGSGMVLGREGPSIQIGGAVGRMI
ASKGKRFSHTTHILTAAGAAAGLAAAFNAPLAGILFVVEEMRPQFRYSITSIKCVTLATAVATIVMRSLHGQAAFLDIPH
FDVPPLRSLILFAVLGILFGVIGVNFNKWILKGTSRVKEYHQNKLSRLVLVGAMFGGTFAFLQLVVPKLSGSGMEIITEM
FHSPMMWMVMVGFFVLRIIVTVACFASGAPGGIFAPMLTLGTLLGLSYGIVMAAWFPEWVSEPGVYAIAGMGALFAATVR
APVTGIILVVEMTNNYQLILPLLVTCLGATFIAQALGGQPLYSALLKQSLLEDKELRVDKNSPASN
>Mature_465_residues
PKSPNFRFFQGGLLKHRTTPKFLTTQTEYFSLLFLSALVGGITGSVVALFEESIRWITEQRLTFVHTLPYAEYIALVAAV
LAGAAMAAVGFWLTFKFAPEVAGSGIPHIEGALDGTHEIRWHRVLPIKFIAGTLTIGSGMVLGREGPSIQIGGAVGRMIA
SKGKRFSHTTHILTAAGAAAGLAAAFNAPLAGILFVVEEMRPQFRYSITSIKCVTLATAVATIVMRSLHGQAAFLDIPHF
DVPPLRSLILFAVLGILFGVIGVNFNKWILKGTSRVKEYHQNKLSRLVLVGAMFGGTFAFLQLVVPKLSGSGMEIITEMF
HSPMMWMVMVGFFVLRIIVTVACFASGAPGGIFAPMLTLGTLLGLSYGIVMAAWFPEWVSEPGVYAIAGMGALFAATVRA
PVTGIILVVEMTNNYQLILPLLVTCLGATFIAQALGGQPLYSALLKQSLLEDKELRVDKNSPASN

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557473, Length=439, Percent_Identity=26.1958997722096, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI189217923, Length=439, Percent_Identity=26.1958997722096, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI189217921, Length=439, Percent_Identity=26.1958997722096, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI153252026, Length=413, Percent_Identity=24.9394673123487, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI55770838, Length=412, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI55770840, Length=412, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI119433677, Length=413, Percent_Identity=26.3922518159806, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786350, Length=437, Percent_Identity=47.3684210526316, Blast_Score=355, Evalue=4e-99,
Organism=Escherichia coli, GI87081943, Length=321, Percent_Identity=29.595015576324, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996455, Length=500, Percent_Identity=23.8, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996452, Length=500, Percent_Identity=23.8, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17532615, Length=400, Percent_Identity=22.5, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17559050, Length=521, Percent_Identity=25.3358925143954, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71980828, Length=485, Percent_Identity=22.2680412371134, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71980824, Length=485, Percent_Identity=22.2680412371134, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71980820, Length=490, Percent_Identity=22.0408163265306, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71985148, Length=217, Percent_Identity=29.4930875576037, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322500, Length=362, Percent_Identity=23.2044198895028, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665008, Length=469, Percent_Identity=26.6524520255864, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45551566, Length=424, Percent_Identity=25.7075471698113, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281361606, Length=456, Percent_Identity=25.4385964912281, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161078208, Length=456, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281361608, Length=456, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573073, Length=456, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573075, Length=456, Percent_Identity=25.4385964912281, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28573071, Length=456, Percent_Identity=25.4385964912281, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 50064; Mature: 49933

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSPNFRFFQGGLLKHRTTPKFLTTQTEYFSLLFLSALVGGITGSVVALFEESIRWITE
CCCCCCCEEECCCCHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLTFVHTLPYAEYIALVAAVLAGAAMAAVGFWLTFKFAPEVAGSGIPHIEGALDGTHEI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCCE
RWHRVLPIKFIAGTLTIGSGMVLGREGPSIQIGGAVGRMIASKGKRFSHTTHILTAAGAA
EEEEEEHHHHHHHHHEECCCEEEECCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGLAAAFNAPLAGILFVVEEMRPQFRYSITSIKCVTLATAVATIVMRSLHGQAAFLDIPH
HHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
FDVPPLRSLILFAVLGILFGVIGVNFNKWILKGTSRVKEYHQNKLSRLVLVGAMFGGTFA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLQLVVPKLSGSGMEIITEMFHSPMMWMVMVGFFVLRIIVTVACFASGAPGGIFAPMLTL
HHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GTLLGLSYGIVMAAWFPEWVSEPGVYAIAGMGALFAATVRAPVTGIILVVEMTNNYQLIL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCEEEEEEEEECCCCEEHH
PLLVTCLGATFIAQALGGQPLYSALLKQSLLEDKELRVDKNSPASN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
PKSPNFRFFQGGLLKHRTTPKFLTTQTEYFSLLFLSALVGGITGSVVALFEESIRWITE
CCCCCCEEECCCCHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRLTFVHTLPYAEYIALVAAVLAGAAMAAVGFWLTFKFAPEVAGSGIPHIEGALDGTHEI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCCE
RWHRVLPIKFIAGTLTIGSGMVLGREGPSIQIGGAVGRMIASKGKRFSHTTHILTAAGAA
EEEEEEHHHHHHHHHEECCCEEEECCCCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGLAAAFNAPLAGILFVVEEMRPQFRYSITSIKCVTLATAVATIVMRSLHGQAAFLDIPH
HHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
FDVPPLRSLILFAVLGILFGVIGVNFNKWILKGTSRVKEYHQNKLSRLVLVGAMFGGTFA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLQLVVPKLSGSGMEIITEMFHSPMMWMVMVGFFVLRIIVTVACFASGAPGGIFAPMLTL
HHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GTLLGLSYGIVMAAWFPEWVSEPGVYAIAGMGALFAATVRAPVTGIILVVEMTNNYQLIL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCEEEEEEEEECCCCEEHH
PLLVTCLGATFIAQALGGQPLYSALLKQSLLEDKELRVDKNSPASN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA