Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 209695596

GI number: 209695596

Start: 2312740

End: 2314683

Strand: Reverse

Name: topB [H]

Synonym: VSAL_I2155

Alternate gene names: 209695596

Gene position: 2314683-2312740 (Counterclockwise)

Preceding gene: 209695597

Following gene: 209695595

Centisome position: 69.61

GC content: 41.56

Gene sequence:

>1944_bases
ATGTCACGATTATTTATTGCAGAAAAACCAAGTTTAGGGCGAGCGATTGCAGCTGGCTTAGCCAAGCCACAAAAAAATCA
CCAAGGCTATATTGAATGTGGTAATGGTGACATTGTGACTTGGTGTATCGGTCACTTATTAGAACAAGTAGAGCCAGATG
CTTATGACGACAAGTATAAATCTTGGCGTATGGAGCATCTGCCAATTGTGCCTGAACAGTGGCAACTTCGACCTAGAAAG
TCATCGAGTAAGCAATTGACGGTGATTCGAAAATTACTTAAAACGGCCTCTCAAGTTATTCACGCGGGAGATCCCGATCG
AGAAGGTCAGTTATTAGTTGATGAAGTCCTTGATTACTTAAAGTTATCGAAGACAAAAAAAGCGGCGACTCAACGTTTGC
TTATTAGTGATTTGAACTTACCCGCGGTAAAACGTGCCTTATCTGCTATGAGAAGTAATCAAGAATTTATTCCTCTTTCT
ATCTCTGCATTAGCTCGTTCTCGTGCTGATTGGCTATATGGCATGAACATGACTCGAGCTTATACTTTGCTAGGGCAACG
AGGTGGATATCAAGGTGTTTTATCAGTAGGCCGAGTACAAACACCCGTTTTGGGACTTGTGACCCGTCGAGATGAAGAGA
TCGCACATTTTATTCCTAACGCGTATTTTACGTTGGACGCGCTTATCCCTTACCATAATCCAAACCTCAATGGTGGATTA
CCCTTTGATATTCGTGCTAAATGGAAACCAAGCGAGGCATGTCAGCAATGGCAAGATGAAGATGGGCGAGTTCTTAATCT
CAAATTAGTCGAAAATGTTGCCCAACGAATTGCGAATCAACCTGCCACAGTGACTAAAGCTGAAAATAAAGAAACGAAGC
AGAATGCGCCTTTGCCGTATTCATTATCTGCATTGCAAATTGATGCATCAAAACGATATAACTTAAGTGCTCAGCAAGTA
CTGGATTCTTGTCAGGCGCTTTATGAAAAGCATAAATTAATTACGTACCCGCGTTCGGACAGCCGTTATTTACCCAAAGA
TCACTATAAACAGCGTGAACAAGTGTGTGCTGCGATCGGGAATAATGACAAGCAGTTATCTAATGCCGTGAATAATGCCG
ATGTAAATTTGCGTTCTCGGGCTTGGAACGACAGTAAAGTAGATGCTCACCATGCGATTATTCCAACCCCTAAATCCAGT
GGGGCTGCAGGGTTATCTGGCTATGAAGAAAAGGTGTATCACCTTATTGCACGTCAGTATTTAATGCAATTTTATCCGGC
CGCTATTTATGCGGAAGCAGAACTTATTTTTGATATTGCGGGTGGTGTATTTTCTGCAAAAGGAAAACAAATGCTTTTTG
CAGGGTGGCGTATATTATTAGGTAAAACACACACCCAAAATGCTGACGATGATGTAGCGAATAAAGTACCTCCATTAAAA
AAAGGGGAAGTACATACGTGTCGTGAGGGAGAGATTAAACACAAAATGACAGAGCCGCCAAAATACTTTACCGAAGCCAC
CTTATTACAAGCAATGACAGGGATAGCACGCTTTGTTGAAGATAAAGAGTTGAAGAAAGTATTAAGGGATACCGATGGTC
TAGGAACAGAAGCGACTCGTGCCGGTATTCTAGATACTCTTTTTAAACGTCAATTATTGATTCGTAATGGAAAAAATATT
AATGCGAGTGACGCGGGAAAAGGCTTAATTCACGCATTGCCTAGAGAAGCAACTTATCCTGATATGACGGCTCATTGGGA
GTTCCAGTTACAAGGCATGACAGAGAAAACGTATTCTTATCGTCCATTTATGCAGTCTCTGGAAACAAAAGTGACTAACT
TTATGCAAGGGGTACAACAAGGTCCGGTTCCTGATTCGCTGAGATCATTAAAAAGTCCGACAAAACCGCAATTTAAACGA
CGAAAAAGCGCAAAGAAATCGTAG

Upstream 100 bases:

>100_bases
CTGCAAATTTATAATAGCGATTAGATTGACTAGAAAACTTGATAACTGTCTATTCGTACAGGTATAGTGCTGATGTTATT
CACAACATCAGCACTTTTTT

Downstream 100 bases:

>100_bases
AGTTAGATCAGTATCATCGAACTTGAAAATATAATCAGTTAATCTTACTTCTCATTTCTAAGCGATTAATATAAGAAGTA
AGTATGACATTTAAGCATGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MSRLFIAEKPSLGRAIAAGLAKPQKNHQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVPEQWQLRPRK
SSSKQLTVIRKLLKTASQVIHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALSAMRSNQEFIPLS
ISALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIAHFIPNAYFTLDALIPYHNPNLNGGL
PFDIRAKWKPSEACQQWQDEDGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSALQIDASKRYNLSAQQV
LDSCQALYEKHKLITYPRSDSRYLPKDHYKQREQVCAAIGNNDKQLSNAVNNADVNLRSRAWNDSKVDAHHAIIPTPKSS
GAAGLSGYEEKVYHLIARQYLMQFYPAAIYAEAELIFDIAGGVFSAKGKQMLFAGWRILLGKTHTQNADDDVANKVPPLK
KGEVHTCREGEIKHKMTEPPKYFTEATLLQAMTGIARFVEDKELKKVLRDTDGLGTEATRAGILDTLFKRQLLIRNGKNI
NASDAGKGLIHALPREATYPDMTAHWEFQLQGMTEKTYSYRPFMQSLETKVTNFMQGVQQGPVPDSLRSLKSPTKPQFKR
RKSAKKS

Sequences:

>Translated_647_residues
MSRLFIAEKPSLGRAIAAGLAKPQKNHQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVPEQWQLRPRK
SSSKQLTVIRKLLKTASQVIHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALSAMRSNQEFIPLS
ISALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIAHFIPNAYFTLDALIPYHNPNLNGGL
PFDIRAKWKPSEACQQWQDEDGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSALQIDASKRYNLSAQQV
LDSCQALYEKHKLITYPRSDSRYLPKDHYKQREQVCAAIGNNDKQLSNAVNNADVNLRSRAWNDSKVDAHHAIIPTPKSS
GAAGLSGYEEKVYHLIARQYLMQFYPAAIYAEAELIFDIAGGVFSAKGKQMLFAGWRILLGKTHTQNADDDVANKVPPLK
KGEVHTCREGEIKHKMTEPPKYFTEATLLQAMTGIARFVEDKELKKVLRDTDGLGTEATRAGILDTLFKRQLLIRNGKNI
NASDAGKGLIHALPREATYPDMTAHWEFQLQGMTEKTYSYRPFMQSLETKVTNFMQGVQQGPVPDSLRSLKSPTKPQFKR
RKSAKKS
>Mature_646_residues
SRLFIAEKPSLGRAIAAGLAKPQKNHQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYKSWRMEHLPIVPEQWQLRPRKS
SSKQLTVIRKLLKTASQVIHAGDPDREGQLLVDEVLDYLKLSKTKKAATQRLLISDLNLPAVKRALSAMRSNQEFIPLSI
SALARSRADWLYGMNMTRAYTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIAHFIPNAYFTLDALIPYHNPNLNGGLP
FDIRAKWKPSEACQQWQDEDGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPYSLSALQIDASKRYNLSAQQVL
DSCQALYEKHKLITYPRSDSRYLPKDHYKQREQVCAAIGNNDKQLSNAVNNADVNLRSRAWNDSKVDAHHAIIPTPKSSG
AAGLSGYEEKVYHLIARQYLMQFYPAAIYAEAELIFDIAGGVFSAKGKQMLFAGWRILLGKTHTQNADDDVANKVPPLKK
GEVHTCREGEIKHKMTEPPKYFTEATLLQAMTGIARFVEDKELKKVLRDTDGLGTEATRAGILDTLFKRQLLIRNGKNIN
ASDAGKGLIHALPREATYPDMTAHWEFQLQGMTEKTYSYRPFMQSLETKVTNFMQGVQQGPVPDSLRSLKSPTKPQFKRR
KSAKKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=636, Percent_Identity=22.3270440251572, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1788061, Length=654, Percent_Identity=55.6574923547401, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=580, Percent_Identity=22.9310344827586, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=513, Percent_Identity=24.7563352826511, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72732; Mature: 72601

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLFIAEKPSLGRAIAAGLAKPQKNHQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYK
CCCEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
SWRMEHLPIVPEQWQLRPRKSSSKQLTVIRKLLKTASQVIHAGDPDREGQLLVDEVLDYL
HCCHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSKTKKAATQRLLISDLNLPAVKRALSAMRSNQEFIPLSISALARSRADWLYGMNMTRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIAHFIPNAYFTLDALIPYHNPNLNGGL
HHHHHCCCCCHHHHHHCCHHCCHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
PFDIRAKWKPSEACQQWQDEDGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPY
CEEEEECCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCE
SLSALQIDASKRYNLSAQQVLDSCQALYEKHKLITYPRSDSRYLPKDHYKQREQVCAAIG
ECEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHC
NNDKQLSNAVNNADVNLRSRAWNDSKVDAHHAIIPTPKSSGAAGLSGYEEKVYHLIARQY
CCHHHHHHHHCCCCCEEHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFYPAAIYAEAELIFDIAGGVFSAKGKQMLFAGWRILLGKTHTQNADDDVANKVPPLK
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHEECCCCCCCCCHHHHHCCCCCC
KGEVHTCREGEIKHKMTEPPKYFTEATLLQAMTGIARFVEDKELKKVLRDTDGLGTEATR
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AGILDTLFKRQLLIRNGKNINASDAGKGLIHALPREATYPDMTAHWEFQLQGMTEKTYSY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHH
RPFMQSLETKVTNFMQGVQQGPVPDSLRSLKSPTKPQFKRRKSAKKS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SRLFIAEKPSLGRAIAAGLAKPQKNHQGYIECGNGDIVTWCIGHLLEQVEPDAYDDKYK
CCEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
SWRMEHLPIVPEQWQLRPRKSSSKQLTVIRKLLKTASQVIHAGDPDREGQLLVDEVLDYL
HCCHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KLSKTKKAATQRLLISDLNLPAVKRALSAMRSNQEFIPLSISALARSRADWLYGMNMTRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
YTLLGQRGGYQGVLSVGRVQTPVLGLVTRRDEEIAHFIPNAYFTLDALIPYHNPNLNGGL
HHHHHCCCCCHHHHHHCCHHCCHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
PFDIRAKWKPSEACQQWQDEDGRVLNLKLVENVAQRIANQPATVTKAENKETKQNAPLPY
CEEEEECCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCE
SLSALQIDASKRYNLSAQQVLDSCQALYEKHKLITYPRSDSRYLPKDHYKQREQVCAAIG
ECEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHC
NNDKQLSNAVNNADVNLRSRAWNDSKVDAHHAIIPTPKSSGAAGLSGYEEKVYHLIARQY
CCHHHHHHHHCCCCCEEHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LMQFYPAAIYAEAELIFDIAGGVFSAKGKQMLFAGWRILLGKTHTQNADDDVANKVPPLK
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHEECCCCCCCCCHHHHHCCCCCC
KGEVHTCREGEIKHKMTEPPKYFTEATLLQAMTGIARFVEDKELKKVLRDTDGLGTEATR
CCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AGILDTLFKRQLLIRNGKNINASDAGKGLIHALPREATYPDMTAHWEFQLQGMTEKTYSY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHH
RPFMQSLETKVTNFMQGVQQGPVPDSLRSLKSPTKPQFKRRKSAKKS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]