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The map label for this gene is dacA [H]

Identifier: 209694545

GI number: 209694545

Start: 1084621

End: 1085796

Strand: Reverse

Name: dacA [H]

Synonym: VSAL_I0982

Alternate gene names: 209694545

Gene position: 1085796-1084621 (Counterclockwise)

Preceding gene: 209694546

Following gene: 209694544

Centisome position: 32.65

GC content: 38.1

Gene sequence:

>1176_bases
ATGAAAAATTCAGCTAAACAGTTAAAACGAATCATCTTATCGAGCTTTATTTTAGGCTCCACCGTTGCTGTAGCTGCTCC
TGCAGTTATTCCAAATGCACCACAAATAGCTGCAAAAGGCTACGTACTAATGGATTATCATTCAGGCAAAGTTCTGGCTG
AAAAAGACATGAATGTTCGTATGAATCCTGCAAGCTTAACCAAAATGATGACAAGCTATGTTATCGGTCAAGAGATTGCG
AGTGGTAATATTTCAATGGATGACGATGTTGTCATCAGTAAGAACGCATGGGCGAAAAACTTCCCAGGTTCTTCTAAAAT
GTTTATTGAAGTCGGTAGCGTTGTTAAAGTTCGTGATTTAAACCAAGGTATTATCGTTCAATCAGGTAATGATGCTTGTG
TGGCAATGGCTGAACATGTTGCTGGTTCAGAAGACGCTTTTGTTGATCTAATGAATGCATGGTCTAAAACATTAAATCTT
CAAAACACACACTTTGCAAACGTTCATGGTTTAGATCGTGACGACCTATATTCAACACCTTATGACATGGCATTAATTGG
CCAAGCATTGATCCGTGATGTACCCGAAGAATATCGCGTATACTCTGAAAAATCATATACTTATAACGGCATCACACAAT
ATAACCGTAATGGCCTATTGTGGGATAAAAGCATGAATGTAGATGGCATCAAAACAGGTCATACTGACGGTGCAGGTTAC
AATCTAGTAAGCTCAGCAACCGAAGGTAAAATGCGTTTAGTTGCTGTAGTAATGGGAACTAAATCAGGTAATGCTCGTAA
AGCAGAAAGCAAAAAATTACTGAATTATGGTTTCCGTTTCTTCGAAACAGTTTCTCCACACAAAGCAAACGAAACTTTCG
TAACAGAAAAAGTATGGATGGGTAGCACGGATGAAGTAGCCCTTGGCGTTGCTGAAGACACTTATGTTACTTTACCTCGT
GGTCAAGCGAAGAAAATGACAGCAAGCTTTGTTCTAGAAAAAACATTGAAAGCACCAATATCTAAAGGTGATGTCGTTGG
TAAACTTTTCTATCAAATTGATGGTGAAGATATTGCTGAGTATCCTCTACTTGCTTTAAATAATGTTGACGAAGGTGGTA
TTTTCAGTCGTCTTATTGATTACATTGTTTTGACATTTAAAGGTTGGTTAAGCTAA

Upstream 100 bases:

>100_bases
TCTCATAGAAAATCAGTTATTTAATTACACATCTGGTTATGATATTCAAATCTGCTATTATGTACGCAGTTTCACTACCT
CAAAGTGCAAAGTTATTATT

Downstream 100 bases:

>100_bases
ACGCTGACTAACTAATTGATTAAGGCGGCTTAGGCAGCCTTTTTTATACCTGATTTTTAGTGATGTAAACTCGATGACTT
ATAACTCTTGTTATCTGACT

Product: penicillin-binding protein 5 precursor (D-alanyl-D-alanin carboxypeptidase fraction A)

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKNSAKQLKRIILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIA
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSVVKVRDLNQGIIVQSGNDACVAMAEHVAGSEDAFVDLMNAWSKTLNL
QNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSYTYNGITQYNRNGLLWDKSMNVDGIKTGHTDGAGY
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLKAPISKGDVVGKLFYQIDGEDIAEYPLLALNNVDEGGIFSRLIDYIVLTFKGWLS

Sequences:

>Translated_391_residues
MKNSAKQLKRIILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIA
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSVVKVRDLNQGIIVQSGNDACVAMAEHVAGSEDAFVDLMNAWSKTLNL
QNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSYTYNGITQYNRNGLLWDKSMNVDGIKTGHTDGAGY
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLKAPISKGDVVGKLFYQIDGEDIAEYPLLALNNVDEGGIFSRLIDYIVLTFKGWLS
>Mature_391_residues
MKNSAKQLKRIILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVRMNPASLTKMMTSYVIGQEIA
SGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSVVKVRDLNQGIIVQSGNDACVAMAEHVAGSEDAFVDLMNAWSKTLNL
QNTHFANVHGLDRDDLYSTPYDMALIGQALIRDVPEEYRVYSEKSYTYNGITQYNRNGLLWDKSMNVDGIKTGHTDGAGY
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWMGSTDEVALGVAEDTYVTLPR
GQAKKMTASFVLEKTLKAPISKGDVVGKLFYQIDGEDIAEYPLLALNNVDEGGIFSRLIDYIVLTFKGWLS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=397, Percent_Identity=54.6599496221663, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787062, Length=379, Percent_Identity=51.7150395778364, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI87082022, Length=382, Percent_Identity=45.2879581151832, Blast_Score=347, Evalue=6e-97,
Organism=Escherichia coli, GI87082054, Length=237, Percent_Identity=26.5822784810127, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42837; Mature: 42837

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSAKQLKRIILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVR
CCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCEE
MNPASLTKMMTSYVIGQEIASGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSVVKVRDL
ECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEC
NQGIIVQSGNDACVAMAEHVAGSEDAFVDLMNAWSKTLNLQNTHFANVHGLDRDDLYSTP
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCC
YDMALIGQALIRDVPEEYRVYSEKSYTYNGITQYNRNGLLWDKSMNVDGIKTGHTDGAGY
CHHHHHHHHHHHHCCHHHHHHCCCCCEECCCEEECCCCEEEECCCCCCCCCCCCCCCCCC
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWM
CHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSTDEVALGVAEDTYVTLPRGQAKKMTASFVLEKTLKAPISKGDVVGKLFYQIDGEDIAE
CCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHC
YPLLALNNVDEGGIFSRLIDYIVLTFKGWLS
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNSAKQLKRIILSSFILGSTVAVAAPAVIPNAPQIAAKGYVLMDYHSGKVLAEKDMNVR
CCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCEE
MNPASLTKMMTSYVIGQEIASGNISMDDDVVISKNAWAKNFPGSSKMFIEVGSVVKVRDL
ECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEEC
NQGIIVQSGNDACVAMAEHVAGSEDAFVDLMNAWSKTLNLQNTHFANVHGLDRDDLYSTP
CCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCC
YDMALIGQALIRDVPEEYRVYSEKSYTYNGITQYNRNGLLWDKSMNVDGIKTGHTDGAGY
CHHHHHHHHHHHHCCHHHHHHCCCCCEECCCEEECCCCEEEECCCCCCCCCCCCCCCCCC
NLVSSATEGKMRLVAVVMGTKSGNARKAESKKLLNYGFRFFETVSPHKANETFVTEKVWM
CHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSTDEVALGVAEDTYVTLPRGQAKKMTASFVLEKTLKAPISKGDVVGKLFYQIDGEDIAE
CCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHC
YPLLALNNVDEGGIFSRLIDYIVLTFKGWLS
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]