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The map label for this gene is tyrS

Identifier: 209694434

GI number: 209694434

Start: 952023

End: 953303

Strand: Direct

Name: tyrS

Synonym: VSAL_I0855

Alternate gene names: 209694434

Gene position: 952023-953303 (Clockwise)

Preceding gene: 209694432

Following gene: 209694437

Centisome position: 28.63

GC content: 40.98

Gene sequence:

>1281_bases
ATGACAGCAACAAATGAATTATTGCAGGATTTAAAAGCTCGTGGGCTTATTGCACAGTGTACAGCAGATGAAGAACTTGC
TGAGCACCTATCTACAGACTGTCGTACTCTCTATTGTGGTTTCGATCCGACAGCAGACAGCTTACATATTGGTAGCCTTG
TTCCTCTTTTAGTGCTTAAACGTTTTCAACAAGCAGGTCATAAGCCATTAGCGCTTGTTGGCGGTGCAACTGGCCTAATT
GGTGACCCAAGCTTTAAAGCGGCTGAGCGTCAATTAAATACATCTGATGTTGTTGGTGATTGGGTTAATAAAATCCGTGC
ACAAGTGTCTGCATTTGTTGATTTTAATGAATCAAAAAATGGCGCAGAAGTGGTGAATAACCTAGATTGGATTGGTGAAA
TCAATGTAATCGAATTCATGCGTGATATCGGCAAGCACTTCAGCGTAAACTCAATGATTCAAAAAGAATCAGTGAAGCAG
CGTATTGACCGTGAAGGTTCTGGTATTTCATTTACTGAATTCAGCTACATGCTACTTCAATCATACGATTTTGCTGCATT
GAATAAAGCGAAAGAATGTACGCTTCAAATCGGTGGTTCTGATCAATGGGGTAACATTACTGGCGGTATCGACCTAACTC
GCCGTATGAACCGTAATAAAGTATTTGGTTTAACGCTACCTCTAGTGACTAAATCCGACGGTACTAAATTCGGCAAAACA
GAATCAGGTACAATTTGGTTAGACCCAAGCAAAACGTCACCATACGCATTCTTCCAATTCTGGTTGGGAACAGCAGATGC
TGATGTATATGATTTCTTACGTTTCTTCACATTCTTATCTGTTGATGAGATTGCTGCTTTTGAAGAGAGTGACAAAAGTG
TACAAGGTCGTCCTGCAGGCCAAGGTGTACTAGCTAAAGAAGTGACTCGCTTAGTGCATGGTGAAGAAGGTTTAGCGTCT
TCTGAGCGCATTACAGCCGCGTTATTCTCTGGTGACTTAGCCTCTCTAACTGAAACCGATTTAGCACAATTAGCACAAGA
TGGTCTACCAACGACTGAACTTGAGGCTTCTGAGCAAACTATCGTTGAAGTACTGACTCAATCTGAACTAGCAAAATCAA
ATAAGATGGCGCGTGAATTTATTGGTAACGGTGCGGTTTCAGTGAATGGTGAAAAAGTGGCTGATGCTGAAGTGATTCTG
AAAAAAGAAGACGCATTATTCGGTAAATACAGCGTAATTAAGCGTGGTAAGAAACTGTTTAACTTATACATCTGGAAATA
A

Upstream 100 bases:

>100_bases
GAAGTGCCTTTTTAGTCACTAACTCTGTTATTTAGCCGCTAAATGTGTAAAATCGAGACTTCTTTTTTATACCGAAATTT
AGAGTACAAGTAGGTTATCT

Downstream 100 bases:

>100_bases
TATTAGCTTCCGTTGATGAGGGATTTGTCTCTCATTAATGGGAGATAAATTATTCATTGTTCAGATAATAAAAAAGCGAA
CCGACAAGGTTCGCTTTTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLI
GDPSFKAAERQLNTSDVVGDWVNKIRAQVSAFVDFNESKNGAEVVNNLDWIGEINVIEFMRDIGKHFSVNSMIQKESVKQ
RIDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
ESGTIWLDPSKTSPYAFFQFWLGTADADVYDFLRFFTFLSVDEIAAFEESDKSVQGRPAGQGVLAKEVTRLVHGEEGLAS
SERITAALFSGDLASLTETDLAQLAQDGLPTTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADAEVIL
KKEDALFGKYSVIKRGKKLFNLYIWK

Sequences:

>Translated_426_residues
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLI
GDPSFKAAERQLNTSDVVGDWVNKIRAQVSAFVDFNESKNGAEVVNNLDWIGEINVIEFMRDIGKHFSVNSMIQKESVKQ
RIDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
ESGTIWLDPSKTSPYAFFQFWLGTADADVYDFLRFFTFLSVDEIAAFEESDKSVQGRPAGQGVLAKEVTRLVHGEEGLAS
SERITAALFSGDLASLTETDLAQLAQDGLPTTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADAEVIL
KKEDALFGKYSVIKRGKKLFNLYIWK
>Mature_425_residues
TATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLKRFQQAGHKPLALVGGATGLIG
DPSFKAAERQLNTSDVVGDWVNKIRAQVSAFVDFNESKNGAEVVNNLDWIGEINVIEFMRDIGKHFSVNSMIQKESVKQR
IDREGSGISFTEFSYMLLQSYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKTE
SGTIWLDPSKTSPYAFFQFWLGTADADVYDFLRFFTFLSVDEIAAFEESDKSVQGRPAGQGVLAKEVTRLVHGEEGLASS
ERITAALFSGDLASLTETDLAQLAQDGLPTTELEASEQTIVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADAEVILK
KEDALFGKYSVIKRGKKLFNLYIWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=440, Percent_Identity=34.3181818181818, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=62.5, Blast_Score=564, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI25145802, Length=440, Percent_Identity=34.7727272727273, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI25145805, Length=317, Percent_Identity=32.807570977918, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325160, Length=372, Percent_Identity=38.1720430107527, Blast_Score=253, Evalue=3e-68,
Organism=Drosophila melanogaster, GI20130383, Length=423, Percent_Identity=37.8250591016548, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_ALISL (B6EHW3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262362.1
- GeneID:   6986687
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0855
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46741; Mature: 46610

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 38-38 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLK
CCCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
RFQQAGHKPLALVGGATGLIGDPSFKAAERQLNTSDVVGDWVNKIRAQVSAFVDFNESKN
HHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCC
GAEVVNNLDWIGEINVIEFMRDIGKHFSVNSMIQKESVKQRIDREGSGISFTEFSYMLLQ
HHHHHHCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
CCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEEEEEEECCCCCCCCCC
ESGTIWLDPSKTSPYAFFQFWLGTADADVYDFLRFFTFLSVDEIAAFEESDKSVQGRPAG
CCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QGVLAKEVTRLVHGEEGLASSERITAALFSGDLASLTETDLAQLAQDGLPTTELEASEQT
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADAEVILKKEDALFGKYSVIKRGKKLF
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCHHHEEECCCHHHHHHHHHHCCHHHH
NLYIWK
EEEECC
>Mature Secondary Structure 
TATNELLQDLKARGLIAQCTADEELAEHLSTDCRTLYCGFDPTADSLHIGSLVPLLVLK
CCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCEEHHHHHHHHHHH
RFQQAGHKPLALVGGATGLIGDPSFKAAERQLNTSDVVGDWVNKIRAQVSAFVDFNESKN
HHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCC
GAEVVNNLDWIGEINVIEFMRDIGKHFSVNSMIQKESVKQRIDREGSGISFTEFSYMLLQ
HHHHHHCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
SYDFAALNKAKECTLQIGGSDQWGNITGGIDLTRRMNRNKVFGLTLPLVTKSDGTKFGKT
CCCHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEEEEEEECCCCCCCCCC
ESGTIWLDPSKTSPYAFFQFWLGTADADVYDFLRFFTFLSVDEIAAFEESDKSVQGRPAG
CCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QGVLAKEVTRLVHGEEGLASSERITAALFSGDLASLTETDLAQLAQDGLPTTELEASEQT
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVEVLTQSELAKSNKMAREFIGNGAVSVNGEKVADAEVILKKEDALFGKYSVIKRGKKLF
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHCCHHHEEECCCHHHHHHHHHHCCHHHH
NLYIWK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA