Click here to switch to the map view.

The map label for this gene is punA [H]

Identifier: 209551746

GI number: 209551746

Start: 4350906

End: 4351706

Strand: Direct

Name: punA [H]

Synonym: Rleg2_4175

Alternate gene names: 209551746

Gene position: 4350906-4351706 (Clockwise)

Preceding gene: 209551745

Following gene: 209551747

Centisome position: 95.88

GC content: 63.55

Gene sequence:

>801_bases
ATGAAGGCGACGGTCGATCTGCTTGCCGCATTGCTCGGCGCCATCAAGCCGCGCCACGGCATCGTGCTCGGCTCCGGCCT
CGGCTCTCTCGTTGGTCAGCTGGAGGGCGCCGTGCGTATTCCCTATCGCGACCTGCCGGGCTTTCCCGTCAGCGCCGTCT
CCGGCCATGCCGGCGAGGTCGTTGCCGGCCGGCTCGGCGGCGTGCCCGTCGTCATGCTCTCCGGCCGCGTGCATTATTAC
GAGAAGGGCGATGCCAACGCCATGCGCCTGCCGATCGAGGTGCTGAAGTCGCTCGGCGTCGAGGCGCTGATCCTGACCAA
TTCGGCCGGATCGCTCCGCGACGACATGCCGCCCGGTTCGGTGATGCAAATCACTGATCACATCAACTATTCCGGCATGA
ACCCGCTGATCGGCGAGGAAAGCGACCACCGTTTCGTCGGCATGACCAATGCTTACGATGCCGGCCTCGCGACGGCGATG
CAGAAGGCAGCGGCAAAGCTCAAAATCGAGCTGGCGCAAGGTGTGTACATGTGGTTCTCCGGCCCGAGCTTCGAAACGCC
GGCCGAAATCCGCATGGCGCGCATCCTCGGTGCCGATGCCGTCGGCATGTCGACGGTGCCGGAGGTGATCATCGCAAGAA
TGCTGGGTCTGAGGGTTGCCGCCGCCTCCGTTATCACCAACTATGGGGCGGGTATGACCGGCAATGAGCTCAGCCATGAA
GAAACCAAGGACATGGCGCCCATCGGCGGTGCCCGTCTCGCCGCCATATTGAAAGAGATGATTGCGGCCAGAACCGGATA
A

Upstream 100 bases:

>100_bases
CCTCCAAGGACACGAAGATCTATCTCTGCGATGAGGCGGGCGTGCAGAAAACCATGACGATGGAAGAGCTCCTTCCCTTC
AGCTTCGAGACCGAACTCGG

Downstream 100 bases:

>100_bases
TTTTAGGCCGGGTCGGCGTAAAGTCTGAATCCTGTTCTACATTAGATAGTGAGAGCATGCTGTCATGAGAAAACCGCTCA
CACTTTTCGGCATCATGCTC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKATVDLLAALLGAIKPRHGIVLGSGLGSLVGQLEGAVRIPYRDLPGFPVSAVSGHAGEVVAGRLGGVPVVMLSGRVHYY
EKGDANAMRLPIEVLKSLGVEALILTNSAGSLRDDMPPGSVMQITDHINYSGMNPLIGEESDHRFVGMTNAYDAGLATAM
QKAAAKLKIELAQGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVIIARMLGLRVAAASVITNYGAGMTGNELSHE
ETKDMAPIGGARLAAILKEMIAARTG

Sequences:

>Translated_266_residues
MKATVDLLAALLGAIKPRHGIVLGSGLGSLVGQLEGAVRIPYRDLPGFPVSAVSGHAGEVVAGRLGGVPVVMLSGRVHYY
EKGDANAMRLPIEVLKSLGVEALILTNSAGSLRDDMPPGSVMQITDHINYSGMNPLIGEESDHRFVGMTNAYDAGLATAM
QKAAAKLKIELAQGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVIIARMLGLRVAAASVITNYGAGMTGNELSHE
ETKDMAPIGGARLAAILKEMIAARTG
>Mature_266_residues
MKATVDLLAALLGAIKPRHGIVLGSGLGSLVGQLEGAVRIPYRDLPGFPVSAVSGHAGEVVAGRLGGVPVVMLSGRVHYY
EKGDANAMRLPIEVLKSLGVEALILTNSAGSLRDDMPPGSVMQITDHINYSGMNPLIGEESDHRFVGMTNAYDAGLATAM
QKAAAKLKIELAQGVYMWFSGPSFETPAEIRMARILGADAVGMSTVPEVIIARMLGLRVAAASVITNYGAGMTGNELSHE
ETKDMAPIGGARLAAILKEMIAARTG

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=223, Percent_Identity=46.6367713004484, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI47132622, Length=234, Percent_Identity=28.6324786324786, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1788746, Length=242, Percent_Identity=47.1074380165289, Blast_Score=215, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17541190, Length=256, Percent_Identity=37.890625, Blast_Score=170, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323238, Length=224, Percent_Identity=44.6428571428571, Blast_Score=172, Evalue=4e-44,
Organism=Drosophila melanogaster, GI45552885, Length=238, Percent_Identity=38.655462184874, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45552887, Length=238, Percent_Identity=38.655462184874, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24656090, Length=238, Percent_Identity=38.655462184874, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24656093, Length=238, Percent_Identity=38.655462184874, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=34.7280334728033, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20130079, Length=229, Percent_Identity=28.82096069869, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459247, Length=206, Percent_Identity=25.7281553398058, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 27764; Mature: 27764

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATVDLLAALLGAIKPRHGIVLGSGLGSLVGQLEGAVRIPYRDLPGFPVSAVSGHAGEV
CCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCEECCCCCCCCCCCHHCCCCCCCE
VAGRLGGVPVVMLSGRVHYYEKGDANAMRLPIEVLKSLGVEALILTNSAGSLRDDMPPGS
EEHHCCCCEEEEEECEEEEEECCCCCEEECHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
VMQITDHINYSGMNPLIGEESDHRFVGMTNAYDAGLATAMQKAAAKLKIELAQGVYMWFS
EEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCCEEEEEC
GPSFETPAEIRMARILGADAVGMSTVPEVIIARMLGLRVAAASVITNYGAGMTGNELSHE
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ETKDMAPIGGARLAAILKEMIAARTG
HHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKATVDLLAALLGAIKPRHGIVLGSGLGSLVGQLEGAVRIPYRDLPGFPVSAVSGHAGEV
CCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCEECCCCCCCCCCCHHCCCCCCCE
VAGRLGGVPVVMLSGRVHYYEKGDANAMRLPIEVLKSLGVEALILTNSAGSLRDDMPPGS
EEHHCCCCEEEEEECEEEEEECCCCCEEECHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
VMQITDHINYSGMNPLIGEESDHRFVGMTNAYDAGLATAMQKAAAKLKIELAQGVYMWFS
EEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEECCCEEEEEC
GPSFETPAEIRMARILGADAVGMSTVPEVIIARMLGLRVAAASVITNYGAGMTGNELSHE
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ETKDMAPIGGARLAAILKEMIAARTG
HHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]