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The map label for this gene is phnE [H]

Identifier: 209551686

GI number: 209551686

Start: 4296543

End: 4297502

Strand: Reverse

Name: phnE [H]

Synonym: Rleg2_4115

Alternate gene names: 209551686

Gene position: 4297502-4296543 (Counterclockwise)

Preceding gene: 209551687

Following gene: 209551685

Centisome position: 94.7

GC content: 60.94

Gene sequence:

>960_bases
ATGACTGTTGCCGATACACAGCCGCACATGCAGAGCACCAGGGATATCCGCACCGCCTGGGAGCGGATGGTCGCCAGGCG
CCGTTTCTACACCATCCTCGGCCTGGTTATCCTGATTGCGGCTTTCGTCAGTTCCGTCCGCTTCGCCGACGAGAGCAATG
CCGGCCATTTCTTCGACCGCCTGCCGCATCTCTTCGACTTTTTGAGCTGGCTCATCCCCAAGGATTGGAACGATGTCTGG
CGCGCGCTGTTCGACATTGCGAGCGTCAACGACAAGGGCGGCGAGGAGTTCAATTTCGACAAGGGCCGCGTCTATATTTC
AGGCGCCTTCTACATTCCCGAATGTTTCGAGCTGATGATCGTCACGATCAATGTGGCGCTGATCTCGACGATCGTCGCCT
TCGTCTTTGCCGTTCCGTTGAGTTTTTTTGCCGCGCGCAACCTGACGAGTTCCTGGCCGCTGCGCGTCTTCACCAAGCGC
CTGATGGAATTCCTGCGCGCCTTCCCCGAGATCGTCATTGCCGGCCTGTTTTCGGCGATCCTGTCGATCGGGCCGGTCGC
CGCCATCATCGCCATCACCCTGCACACGATCGGCGCGCTCGGCAAACTCTTCTACGAAGTGGCAGAGAATATCGACATGA
AGCCGCATGAGGGCATGAAGGCCGTCGGCGCCAACTGGTGGGAGCGCGTGCGCTTCGCGGCTCTGCCGCAGGTGCTACCG
AATTTCACCTCCTATGCGCTCTTGCGCCTCGAGATCAACGTGCGTGCCTCGACCATCATCGGCGCCGTCGGCGGCGGCGG
TATCGGCGAGGAACTGAAGCTTTCGATCTCGCGCGGCTTCGGCGCCAAGACGATGGCGCTGGTTCTGCTGCTGTTCGTGA
CGATCGTCGCCGTCGACCAGTTCTCCGCATGGCTGCGCCGCCGCCTCGTCGGCGAGCATGCCTTCCTCCTGCAACATTGA

Upstream 100 bases:

>100_bases
TCGTCGAAGTGCGTAAGGCTGCCATCGGCGGCTGATCGGCCGTTGGAATGCGGGGCGGCCGGAAACGGCCGCCCTTTATC
TTCGATCGGGCGAGGCGTCC

Downstream 100 bases:

>100_bases
GGCTGCCATGACGGTGATCGACGCAAACCGCATGAACGAAATCGAAGCGCGCTATCCGGAATATTTCCACCGGTCGTTCC
GCCAGCGTTTCGGCGGGCTG

Product: phosphonate ABC transporter inner membrane subunit

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTVADTQPHMQSTRDIRTAWERMVARRRFYTILGLVILIAAFVSSVRFADESNAGHFFDRLPHLFDFLSWLIPKDWNDVW
RALFDIASVNDKGGEEFNFDKGRVYISGAFYIPECFELMIVTINVALISTIVAFVFAVPLSFFAARNLTSSWPLRVFTKR
LMEFLRAFPEIVIAGLFSAILSIGPVAAIIAITLHTIGALGKLFYEVAENIDMKPHEGMKAVGANWWERVRFAALPQVLP
NFTSYALLRLEINVRASTIIGAVGGGGIGEELKLSISRGFGAKTMALVLLLFVTIVAVDQFSAWLRRRLVGEHAFLLQH

Sequences:

>Translated_319_residues
MTVADTQPHMQSTRDIRTAWERMVARRRFYTILGLVILIAAFVSSVRFADESNAGHFFDRLPHLFDFLSWLIPKDWNDVW
RALFDIASVNDKGGEEFNFDKGRVYISGAFYIPECFELMIVTINVALISTIVAFVFAVPLSFFAARNLTSSWPLRVFTKR
LMEFLRAFPEIVIAGLFSAILSIGPVAAIIAITLHTIGALGKLFYEVAENIDMKPHEGMKAVGANWWERVRFAALPQVLP
NFTSYALLRLEINVRASTIIGAVGGGGIGEELKLSISRGFGAKTMALVLLLFVTIVAVDQFSAWLRRRLVGEHAFLLQH
>Mature_318_residues
TVADTQPHMQSTRDIRTAWERMVARRRFYTILGLVILIAAFVSSVRFADESNAGHFFDRLPHLFDFLSWLIPKDWNDVWR
ALFDIASVNDKGGEEFNFDKGRVYISGAFYIPECFELMIVTINVALISTIVAFVFAVPLSFFAARNLTSSWPLRVFTKRL
MEFLRAFPEIVIAGLFSAILSIGPVAAIIAITLHTIGALGKLFYEVAENIDMKPHEGMKAVGANWWERVRFAALPQVLPN
FTSYALLRLEINVRASTIIGAVGGGGIGEELKLSISRGFGAKTMALVLLLFVTIVAVDQFSAWLRRRLVGEHAFLLQH

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35759; Mature: 35628

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVADTQPHMQSTRDIRTAWERMVARRRFYTILGLVILIAAFVSSVRFADESNAGHFFDR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LPHLFDFLSWLIPKDWNDVWRALFDIASVNDKGGEEFNFDKGRVYISGAFYIPECFELMI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHH
VTINVALISTIVAFVFAVPLSFFAARNLTSSWPLRVFTKRLMEFLRAFPEIVIAGLFSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIGPVAAIIAITLHTIGALGKLFYEVAENIDMKPHEGMKAVGANWWERVRFAALPQVLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NFTSYALLRLEINVRASTIIGAVGGGGIGEELKLSISRGFGAKTMALVLLLFVTIVAVDQ
CHHHHEEEEEEEEEEHHHHEECCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHH
FSAWLRRRLVGEHAFLLQH
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVADTQPHMQSTRDIRTAWERMVARRRFYTILGLVILIAAFVSSVRFADESNAGHFFDR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LPHLFDFLSWLIPKDWNDVWRALFDIASVNDKGGEEFNFDKGRVYISGAFYIPECFELMI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHH
VTINVALISTIVAFVFAVPLSFFAARNLTSSWPLRVFTKRLMEFLRAFPEIVIAGLFSAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIGPVAAIIAITLHTIGALGKLFYEVAENIDMKPHEGMKAVGANWWERVRFAALPQVLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NFTSYALLRLEINVRASTIIGAVGGGGIGEELKLSISRGFGAKTMALVLLLFVTIVAVDQ
CHHHHEEEEEEEEEEHHHHEECCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHHHHHH
FSAWLRRRLVGEHAFLLQH
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]