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The map label for this gene is phnE [H]

Identifier: 209551685

GI number: 209551685

Start: 4295192

End: 4296535

Strand: Reverse

Name: phnE [H]

Synonym: Rleg2_4114

Alternate gene names: 209551685

Gene position: 4296535-4295192 (Counterclockwise)

Preceding gene: 209551686

Following gene: 209551684

Centisome position: 94.68

GC content: 61.31

Gene sequence:

>1344_bases
ATGACGGTGATCGACGCAAACCGCATGAACGAAATCGAAGCGCGCTATCCGGAATATTTCCACCGGTCGTTCCGCCAGCG
TTTCGGCGGGCTGATGATCCTGATCGCGACGCTGCTCTACAGCCTCTATGCCGTCTGGTTCTTCGACCTGCCCAAGCTTT
TCGCCGAAGCCCATTGGGAGCGCGTCGGCATCTATCTCAGCCAATGGGTGAGCTACGACGTTCAGCCGGAATTCCGCATC
GAGGACGACGGCTCGATCGACATCCGCTATCCGCGCTTCTCGCCGCTCGGCGACAATCCGCATCCGGACTGGCTGGTCAA
CAATCCCGATGGCAGCATCACCGTTTCGATCAGCGGCACCAGCCGCACCGTCACCGTTTCGAAGAGCCAGACGATCGTCA
CCGCCCACGGCATCAGCGTCCCGGTCGATGTTTCGAGCGGTGCGCCGAAAGTCGTCGGACCAGTGCCCGGCTGGATGACC
GTCTATGATGACAATGTGCTCGCCGATCTCGGCTTTGCCGGCAATGTCAGCATCTCCGTCGATCGGGTGAAAATCCGCAA
GCGTTTCATCGGCTGGGCGAATTTCATCTTCGACACGCAATCGTCCTTCTTCGACAAGCCGGTCGGCGAGGTCGTCGGCC
TGATCGTCTCCGGGCCGCGCATCAAGCCCGACCAGTCCAATCTGTCGCTCGCCTTCGACGACATCTGGAACAACAGTGAA
TGGCAGCACGGCGACGTCTGGACCAAGCTTTTCCAGACGATCGTGATGGCATTCCTCGGCACGCTGCTGGGGTCGCTCAC
CGCCTTCCCGCTCGCCTTCCTGGCGGCGCGCAATATCACGCCGAACCGGCTGCTCAATCAGATCCTGAAGCGCTTTTTCG
ATTTCCTGCGCTCGGTGGACATGCTGATCTGGGCGCTGTTCCTGACGCGCGCCTTCGGGCCCGGCCCGCTTGCCGGCAGC
GGCGCGATCTTCCTCACCGAGACCGGCACGCTCGGCAAGCTCTATTCCGAAGGGCTGGAGAATATCGACAACAAGCCGCG
GGAAGGCATCAAATCGACCGGCGCCCAGCCGGTGCTTGTCCAGCGTTACGGCATTATGCCGCAGATCGTTCCCGTCATCG
TCAGCCAGACGCTCTACCAATGGGAATCGAACGTGCGCGGCGCGACCATCATCGGCGCCGTCGGCGCGGGCGGCATCGGG
CTTAAATTGTGGGAGGCGATGCGCACCAACGCCAACTGGGAAAACGTTGCCTACATGGTCATCCTGATCCTGATCGTCGT
CTTCCTTTTCGACGCCGCTTCGAACGCGCTGCGCCACCGGCTGATGGGCACGAAAACCCACTGA

Upstream 100 bases:

>100_bases
CTGCTGTTCGTGACGATCGTCGCCGTCGACCAGTTCTCCGCATGGCTGCGCCGCCGCCTCGTCGGCGAGCATGCCTTCCT
CCTGCAACATTGAGGCTGCC

Downstream 100 bases:

>100_bases
TTTATCCGCTCGAGAGGTCGTGGCGGCTGCTCCCCATCATCATTCTGTCACGGAAATCTTTTAGCCGGGAGCTGCTTGCG
GTTCGCCGCCAAATCACTGC

Product: phosphonate ABC transporter inner membrane subunit

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTVIDANRMNEIEARYPEYFHRSFRQRFGGLMILIATLLYSLYAVWFFDLPKLFAEAHWERVGIYLSQWVSYDVQPEFRI
EDDGSIDIRYPRFSPLGDNPHPDWLVNNPDGSITVSISGTSRTVTVSKSQTIVTAHGISVPVDVSSGAPKVVGPVPGWMT
VYDDNVLADLGFAGNVSISVDRVKIRKRFIGWANFIFDTQSSFFDKPVGEVVGLIVSGPRIKPDQSNLSLAFDDIWNNSE
WQHGDVWTKLFQTIVMAFLGTLLGSLTAFPLAFLAARNITPNRLLNQILKRFFDFLRSVDMLIWALFLTRAFGPGPLAGS
GAIFLTETGTLGKLYSEGLENIDNKPREGIKSTGAQPVLVQRYGIMPQIVPVIVSQTLYQWESNVRGATIIGAVGAGGIG
LKLWEAMRTNANWENVAYMVILILIVVFLFDAASNALRHRLMGTKTH

Sequences:

>Translated_447_residues
MTVIDANRMNEIEARYPEYFHRSFRQRFGGLMILIATLLYSLYAVWFFDLPKLFAEAHWERVGIYLSQWVSYDVQPEFRI
EDDGSIDIRYPRFSPLGDNPHPDWLVNNPDGSITVSISGTSRTVTVSKSQTIVTAHGISVPVDVSSGAPKVVGPVPGWMT
VYDDNVLADLGFAGNVSISVDRVKIRKRFIGWANFIFDTQSSFFDKPVGEVVGLIVSGPRIKPDQSNLSLAFDDIWNNSE
WQHGDVWTKLFQTIVMAFLGTLLGSLTAFPLAFLAARNITPNRLLNQILKRFFDFLRSVDMLIWALFLTRAFGPGPLAGS
GAIFLTETGTLGKLYSEGLENIDNKPREGIKSTGAQPVLVQRYGIMPQIVPVIVSQTLYQWESNVRGATIIGAVGAGGIG
LKLWEAMRTNANWENVAYMVILILIVVFLFDAASNALRHRLMGTKTH
>Mature_446_residues
TVIDANRMNEIEARYPEYFHRSFRQRFGGLMILIATLLYSLYAVWFFDLPKLFAEAHWERVGIYLSQWVSYDVQPEFRIE
DDGSIDIRYPRFSPLGDNPHPDWLVNNPDGSITVSISGTSRTVTVSKSQTIVTAHGISVPVDVSSGAPKVVGPVPGWMTV
YDDNVLADLGFAGNVSISVDRVKIRKRFIGWANFIFDTQSSFFDKPVGEVVGLIVSGPRIKPDQSNLSLAFDDIWNNSEW
QHGDVWTKLFQTIVMAFLGTLLGSLTAFPLAFLAARNITPNRLLNQILKRFFDFLRSVDMLIWALFLTRAFGPGPLAGSG
AIFLTETGTLGKLYSEGLENIDNKPREGIKSTGAQPVLVQRYGIMPQIVPVIVSQTLYQWESNVRGATIIGAVGAGGIGL
KLWEAMRTNANWENVAYMVILILIVVFLFDAASNALRHRLMGTKTH

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 49828; Mature: 49697

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIDANRMNEIEARYPEYFHRSFRQRFGGLMILIATLLYSLYAVWFFDLPKLFAEAHWE
CCEECCCCCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGIYLSQWVSYDVQPEFRIEDDGSIDIRYPRFSPLGDNPHPDWLVNNPDGSITVSISGT
HHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEECC
SRTVTVSKSQTIVTAHGISVPVDVSSGAPKVVGPVPGWMTVYDDNVLADLGFAGNVSISV
CEEEEEECCCEEEEECCCEEEEECCCCCCCEECCCCCCEEEECCCEEEECCCCCCEEEEE
DRVKIRKRFIGWANFIFDTQSSFFDKPVGEVVGLIVSGPRIKPDQSNLSLAFDDIWNNSE
HHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCC
WQHGDVWTKLFQTIVMAFLGTLLGSLTAFPLAFLAARNITPNRLLNQILKRFFDFLRSVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MLIWALFLTRAFGPGPLAGSGAIFLTETGTLGKLYSEGLENIDNKPREGIKSTGAQPVLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHH
QRYGIMPQIVPVIVSQTLYQWESNVRGATIIGAVGAGGIGLKLWEAMRTNANWENVAYMV
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHH
ILILIVVFLFDAASNALRHRLMGTKTH
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVIDANRMNEIEARYPEYFHRSFRQRFGGLMILIATLLYSLYAVWFFDLPKLFAEAHWE
CEECCCCCHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGIYLSQWVSYDVQPEFRIEDDGSIDIRYPRFSPLGDNPHPDWLVNNPDGSITVSISGT
HHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCEEEECCCCEEEEEEECC
SRTVTVSKSQTIVTAHGISVPVDVSSGAPKVVGPVPGWMTVYDDNVLADLGFAGNVSISV
CEEEEEECCCEEEEECCCEEEEECCCCCCCEECCCCCCEEEECCCEEEECCCCCCEEEEE
DRVKIRKRFIGWANFIFDTQSSFFDKPVGEVVGLIVSGPRIKPDQSNLSLAFDDIWNNSE
HHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCC
WQHGDVWTKLFQTIVMAFLGTLLGSLTAFPLAFLAARNITPNRLLNQILKRFFDFLRSVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MLIWALFLTRAFGPGPLAGSGAIFLTETGTLGKLYSEGLENIDNKPREGIKSTGAQPVLV
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHH
QRYGIMPQIVPVIVSQTLYQWESNVRGATIIGAVGAGGIGLKLWEAMRTNANWENVAYMV
HHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHH
ILILIVVFLFDAASNALRHRLMGTKTH
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]