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The map label for this gene is ydfI [H]

Identifier: 209551593

GI number: 209551593

Start: 4194192

End: 4195649

Strand: Reverse

Name: ydfI [H]

Synonym: Rleg2_4022

Alternate gene names: 209551593

Gene position: 4195649-4194192 (Counterclockwise)

Preceding gene: 209551594

Following gene: 209551585

Centisome position: 92.46

GC content: 63.44

Gene sequence:

>1458_bases
GTGAGCGAACGACTGCAGACCCTTTCCGGCCTCGCCCCGACGGCGAAGCTTCCCGCCTATGATCGCAGCCGGCTGAAAAG
CGGCATCCTGCATCTGGGGCCCGGCGCCTTTTTCCGTGCGCATTTCGCGCCGTTTACGGATGGCGCGCTCGCAGCCGACG
GCGGCGACTGGGGCATCGAGGTGGCAAGCCTGCGCACCGCCGATGTCGCCGACAATCTGAGTGCCCAGAACGGGCTCTAT
ACGACGTTGATCCGCGACACTTCGGGCACGACGGCTGAGGTGATCGGCTCGATCCTGAAGGCGCATGTGGCGCCGCGCGA
TCCGGCCGGCCTGCTGGCGCGGCTCGAAGATCCCGATATCCGCATCGTCAGCATGACGGTGACGGAAAAGGCCTATGGTT
TCGATCCCGCAACCGGCGGCCTCGACCTGAAACATCCCGATATCGTCGCCGACCTCGCCAACCGCCATGCACCGCGCGGC
GTCATCGGCTATCTCGTCGAGGGCCTTGCGCGCCGCCGGCAGAAGGGGATTGCGCCTTTTACGCCGCTCAGCTGCGACAA
CCTGCCGAGTAACGGCGCTGTTTTGAAACGCCTGGTGCTCGAATTCACCTCCCGTATCGATGCCGATCTGCATCGCTGGA
TCGAAGCGAATGTGCCCTTCCCGTCGACGATGGTCGACCGCATCACGCCGGCCAGCACCGACGCGACCTACGCCGATGCC
GAGCGGCTGACAGGCCGCACGGATATGGCGGCGATCGAGACGGAACCTTTTACGCAGTGGGTGATCGAAGACCATTTCGC
CAATGGCCGGCCGGCCTGGGAAAAGGTGCGCGGCGCGCTGATGGTCGAAGACGTCTCGGCCTATGAGAAGATGAAGCTCC
GGATGCTGAACGGCGCCCATTCGCTGCTCGCCTATCTCGGATATATCGGCGGCTATGAATTTATCCGCGACGTGATGGAC
GATGCCGCCCTGGCGGCACTTGCCTACCGCCACATGCATGCGGCGGCGAGAACGCTCGATCCGGTTCCCGGCATCGATCT
CGACGACTATGCCAATGAATTGATAGCACGCTTTGCAAACAAGGCGATTGCGCATCGCACCTACCAGATCGCCATGGACG
GCACACAGAAACTGCCGCAACGGCTGCTGGAACCGGCATGCGAAGCGCTGGCGCATGGCGATCGGGCGGAAACCTTTGCA
ATCGCCGTTGCGGCATGGATGCGTTATGCGCTCGGCGAGCACGGCAATGGCGAGCGCTACGAGCTGCGCGATCCCCGTGC
CGCGGAGATCGCCGCGCTGATTGCCGATGTCCCGCGCACAGGTCCGGCGATTTCGGCGGCGCTTTTTGCCCTGCCCGGCC
TCTTCCCCTCGGCTTTGACCGGACATCGGGCTTGGACACAGGATGTGTCGGATAAGCTGGAGATCCTGATCCAAGACGAT
CGGTTGCCGTTGTTTTGA

Upstream 100 bases:

>100_bases
TCCTCGCCGGGCTCGCCGCCGAGCACCGGATTTCCAAGAAGGAAGCCGAGATCGTGGCGGGCGAACTCTCCTATGGCAAT
GCGAAGAAGGCCTACAAGCT

Downstream 100 bases:

>100_bases
GGTTTTCCGACATAGGAGAGGGCGTGCCGTGGCCGCCCCCCTCTGCCCTGCCGGGCATCTCCCCCACAGGTGGGGAGATT
GGCTGGGCTCACTGGCTTCC

Product: Mannitol dehydrogenase domain

Products: D-fructuronate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSERLQTLSGLAPTAKLPAYDRSRLKSGILHLGPGAFFRAHFAPFTDGALAADGGDWGIEVASLRTADVADNLSAQNGLY
TTLIRDTSGTTAEVIGSILKAHVAPRDPAGLLARLEDPDIRIVSMTVTEKAYGFDPATGGLDLKHPDIVADLANRHAPRG
VIGYLVEGLARRRQKGIAPFTPLSCDNLPSNGAVLKRLVLEFTSRIDADLHRWIEANVPFPSTMVDRITPASTDATYADA
ERLTGRTDMAAIETEPFTQWVIEDHFANGRPAWEKVRGALMVEDVSAYEKMKLRMLNGAHSLLAYLGYIGGYEFIRDVMD
DAALAALAYRHMHAAARTLDPVPGIDLDDYANELIARFANKAIAHRTYQIAMDGTQKLPQRLLEPACEALAHGDRAETFA
IAVAAWMRYALGEHGNGERYELRDPRAAEIAALIADVPRTGPAISAALFALPGLFPSALTGHRAWTQDVSDKLEILIQDD
RLPLF

Sequences:

>Translated_485_residues
MSERLQTLSGLAPTAKLPAYDRSRLKSGILHLGPGAFFRAHFAPFTDGALAADGGDWGIEVASLRTADVADNLSAQNGLY
TTLIRDTSGTTAEVIGSILKAHVAPRDPAGLLARLEDPDIRIVSMTVTEKAYGFDPATGGLDLKHPDIVADLANRHAPRG
VIGYLVEGLARRRQKGIAPFTPLSCDNLPSNGAVLKRLVLEFTSRIDADLHRWIEANVPFPSTMVDRITPASTDATYADA
ERLTGRTDMAAIETEPFTQWVIEDHFANGRPAWEKVRGALMVEDVSAYEKMKLRMLNGAHSLLAYLGYIGGYEFIRDVMD
DAALAALAYRHMHAAARTLDPVPGIDLDDYANELIARFANKAIAHRTYQIAMDGTQKLPQRLLEPACEALAHGDRAETFA
IAVAAWMRYALGEHGNGERYELRDPRAAEIAALIADVPRTGPAISAALFALPGLFPSALTGHRAWTQDVSDKLEILIQDD
RLPLF
>Mature_484_residues
SERLQTLSGLAPTAKLPAYDRSRLKSGILHLGPGAFFRAHFAPFTDGALAADGGDWGIEVASLRTADVADNLSAQNGLYT
TLIRDTSGTTAEVIGSILKAHVAPRDPAGLLARLEDPDIRIVSMTVTEKAYGFDPATGGLDLKHPDIVADLANRHAPRGV
IGYLVEGLARRRQKGIAPFTPLSCDNLPSNGAVLKRLVLEFTSRIDADLHRWIEANVPFPSTMVDRITPASTDATYADAE
RLTGRTDMAAIETEPFTQWVIEDHFANGRPAWEKVRGALMVEDVSAYEKMKLRMLNGAHSLLAYLGYIGGYEFIRDVMDD
AALAALAYRHMHAAARTLDPVPGIDLDDYANELIARFANKAIAHRTYQIAMDGTQKLPQRLLEPACEALAHGDRAETFAI
AVAAWMRYALGEHGNGERYELRDPRAAEIAALIADVPRTGPAISAALFALPGLFPSALTGHRAWTQDVSDKLEILIQDDR
LPLF

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=463, Percent_Identity=45.1403887688985, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788497, Length=471, Percent_Identity=43.9490445859873, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790779, Length=448, Percent_Identity=46.6517857142857, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI48994885, Length=410, Percent_Identity=25.1219512195122, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790028, Length=307, Percent_Identity=27.0358306188925, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324401, Length=433, Percent_Identity=38.7990762124711, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6320765, Length=433, Percent_Identity=38.7990762124711, Blast_Score=311, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.57

Molecular weight: Translated: 52766; Mature: 52634

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERLQTLSGLAPTAKLPAYDRSRLKSGILHLGPGAFFRAHFAPFTDGALAADGGDWGIE
CCHHHHHHHCCCCCCCCCCHHHHHHHCCCEECCCCHHHHHHCCCCCCCCEEECCCCCCEE
VASLRTADVADNLSAQNGLYTTLIRDTSGTTAEVIGSILKAHVAPRDPAGLLARLEDPDI
EEEEEHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCE
RIVSMTVTEKAYGFDPATGGLDLKHPDIVADLANRHAPRGVIGYLVEGLARRRQKGIAPF
EEEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
TPLSCDNLPSNGAVLKRLVLEFTSRIDADLHRWIEANVPFPSTMVDRITPASTDATYADA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHH
ERLTGRTDMAAIETEPFTQWVIEDHFANGRPAWEKVRGALMVEDVSAYEKMKLRMLNGAH
HHHCCCCCHHEECCCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SLLAYLGYIGGYEFIRDVMDDAALAALAYRHMHAAARTLDPVPGIDLDDYANELIARFAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAIAHRTYQIAMDGTQKLPQRLLEPACEALAHGDRAETFAIAVAAWMRYALGEHGNGERY
HHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
ELRDPRAAEIAALIADVPRTGPAISAALFALPGLFPSALTGHRAWTQDVSDKLEILIQDD
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHEEEEECC
RLPLF
CCCCC
>Mature Secondary Structure 
SERLQTLSGLAPTAKLPAYDRSRLKSGILHLGPGAFFRAHFAPFTDGALAADGGDWGIE
CHHHHHHHCCCCCCCCCCHHHHHHHCCCEECCCCHHHHHHCCCCCCCCEEECCCCCCEE
VASLRTADVADNLSAQNGLYTTLIRDTSGTTAEVIGSILKAHVAPRDPAGLLARLEDPDI
EEEEEHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHEECCCCCE
RIVSMTVTEKAYGFDPATGGLDLKHPDIVADLANRHAPRGVIGYLVEGLARRRQKGIAPF
EEEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
TPLSCDNLPSNGAVLKRLVLEFTSRIDADLHRWIEANVPFPSTMVDRITPASTDATYADA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHH
ERLTGRTDMAAIETEPFTQWVIEDHFANGRPAWEKVRGALMVEDVSAYEKMKLRMLNGAH
HHHCCCCCHHEECCCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SLLAYLGYIGGYEFIRDVMDDAALAALAYRHMHAAARTLDPVPGIDLDDYANELIARFAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAIAHRTYQIAMDGTQKLPQRLLEPACEALAHGDRAETFAIAVAAWMRYALGEHGNGERY
HHHHHHEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
ELRDPRAAEIAALIADVPRTGPAISAALFALPGLFPSALTGHRAWTQDVSDKLEILIQDD
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHEEEEECC
RLPLF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD+

Specific reaction: D-mannonate + NAD+ = D-fructuronate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]