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The map label for this gene is folC [H]

Identifier: 209551528

GI number: 209551528

Start: 4105418

End: 4106746

Strand: Direct

Name: folC [H]

Synonym: Rleg2_3957

Alternate gene names: 209551528

Gene position: 4105418-4106746 (Clockwise)

Preceding gene: 209551527

Following gene: 209551541

Centisome position: 90.47

GC content: 62.3

Gene sequence:

>1329_bases
GTGAGTGAGGCAGCACAGGAGATCGACAAGCTCATGGGATTGCATCCCAAGGGTTTTGATCTGTCTCTCGATCGCATCAC
CCGTCTACTCGATGTGCTCGGCAATCCGCACAGGAAACTGCCGCCGGTGATCCATGTCGCCGGCACCAACGGCAAGGGGT
CGGTCACCGCCTTCTGCCGCGCTCTGCTCGAAGCCGGCGGCTACAGCGCCCATGTTCACACCTCGCCGCATCTCGTCAAC
TGGCACGAGCGTTATCGCATCGGTGTGGCGGGCGGCCGCGGGCAGCTTGTCGATGACGCCGTCTTTGCCGAGGCCTTGCG
CCGTGTGGCCGATGCCAATGCCGGACAGCACATCACCGTCTTCGAAATCCTGACGGCGGTCACCTTCATCCTGTTTTCCG
AACAGCCGGCCGATGCCGCGATCATCGAAGTCGGCCTCGGCGGCCGGTTCGATGCCACCAATGTCATTACCGATCCGGGC
GTCTCGGTGATCATGCCGATCTCGCTGGATCACCAGCCCTATCTCGGCGACAGGGTCGAGCTGATCGCCGCCGAAAAGGC
CGGCATCATGAAGCCGGGACTTCCCGTCGTCATCGGTCATCAGGAATATGATGCAGCCCTCGACGTGCTGATGTCGACGG
CCGAACGGCTGCATTGCCCGAGCGCCGTCTTCGGGCAGGATTTCATGGCGCATGAGGAATATGGCCGGCTCGTCTATCAG
GACGAATTCGGTCTGGCCGACCTGCCGCTGCCGCGCCTTCCCGGCCGCCATCAATATGCCAATGCCGCCGCTGCCATCCG
CGCCGTCAAGGCGGCGGGCTTCACCGTTACCGAGACGATGATGGAAAAGGCGATGAATTCGGTGGAATGGCCGGGCCGGC
TGCAGCGTCTCGGCGAAGGCCGGCTGCTGCAACATGCACCCGCCGGTGCTGAGATCTGGATCGATGGCGGGCACAATCCC
GGGGCCGGCGAAGTGATCGCCGAAGCCATGGCCAATTTCGAAGAGCGCCAGTCTCGGCCGCTTTTTCTGATCATCGGCAT
GATCAACACCAAGGATCCGGTCGGCTATTTCAAGGCCTTTGCCGGCTTGGTCGAGAAGGTTTTCTGCGTGCCGATCCGCA
ACAGCGATGCGATGATCGACCCGGTGATCTTGTCGAACGCCGCCTATGATGCCGGCCTGGTTGCCGAACCGATGTCGACC
GTCGGCGATGCGCTGGAAGCCATAAAAGCAGTCCTCGATCCGGAGGGTTTACCCCCGCGCATCCTCGTCGGCGGCTCTCT
CTATCTCGTCGGCGATGTGCTTTCCGACAACGGCACGCCTCCGAAGTGA

Upstream 100 bases:

>100_bases
GGCGAACAACATGAATAGCGGCGCGATCGCCCTGGCGGCGAGCGCCTGATAACCGATAAGCAGGCGGGGTGCCGAATTGA
TACCCAGAGGCCAAACCGCG

Downstream 100 bases:

>100_bases
TCGGTGAAATGAACAATACGAAATGAAAAAAGCCCGGTCGAAGCCGGGCTTTTTCGTCGATGTGAATCGGGCGATATCAG
GCGGCGCTGGAAATCCAGTT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSEAAQEIDKLMGLHPKGFDLSLDRITRLLDVLGNPHRKLPPVIHVAGTNGKGSVTAFCRALLEAGGYSAHVHTSPHLVN
WHERYRIGVAGGRGQLVDDAVFAEALRRVADANAGQHITVFEILTAVTFILFSEQPADAAIIEVGLGGRFDATNVITDPG
VSVIMPISLDHQPYLGDRVELIAAEKAGIMKPGLPVVIGHQEYDAALDVLMSTAERLHCPSAVFGQDFMAHEEYGRLVYQ
DEFGLADLPLPRLPGRHQYANAAAAIRAVKAAGFTVTETMMEKAMNSVEWPGRLQRLGEGRLLQHAPAGAEIWIDGGHNP
GAGEVIAEAMANFEERQSRPLFLIIGMINTKDPVGYFKAFAGLVEKVFCVPIRNSDAMIDPVILSNAAYDAGLVAEPMST
VGDALEAIKAVLDPEGLPPRILVGGSLYLVGDVLSDNGTPPK

Sequences:

>Translated_442_residues
MSEAAQEIDKLMGLHPKGFDLSLDRITRLLDVLGNPHRKLPPVIHVAGTNGKGSVTAFCRALLEAGGYSAHVHTSPHLVN
WHERYRIGVAGGRGQLVDDAVFAEALRRVADANAGQHITVFEILTAVTFILFSEQPADAAIIEVGLGGRFDATNVITDPG
VSVIMPISLDHQPYLGDRVELIAAEKAGIMKPGLPVVIGHQEYDAALDVLMSTAERLHCPSAVFGQDFMAHEEYGRLVYQ
DEFGLADLPLPRLPGRHQYANAAAAIRAVKAAGFTVTETMMEKAMNSVEWPGRLQRLGEGRLLQHAPAGAEIWIDGGHNP
GAGEVIAEAMANFEERQSRPLFLIIGMINTKDPVGYFKAFAGLVEKVFCVPIRNSDAMIDPVILSNAAYDAGLVAEPMST
VGDALEAIKAVLDPEGLPPRILVGGSLYLVGDVLSDNGTPPK
>Mature_441_residues
SEAAQEIDKLMGLHPKGFDLSLDRITRLLDVLGNPHRKLPPVIHVAGTNGKGSVTAFCRALLEAGGYSAHVHTSPHLVNW
HERYRIGVAGGRGQLVDDAVFAEALRRVADANAGQHITVFEILTAVTFILFSEQPADAAIIEVGLGGRFDATNVITDPGV
SVIMPISLDHQPYLGDRVELIAAEKAGIMKPGLPVVIGHQEYDAALDVLMSTAERLHCPSAVFGQDFMAHEEYGRLVYQD
EFGLADLPLPRLPGRHQYANAAAAIRAVKAAGFTVTETMMEKAMNSVEWPGRLQRLGEGRLLQHAPAGAEIWIDGGHNPG
AGEVIAEAMANFEERQSRPLFLIIGMINTKDPVGYFKAFAGLVEKVFCVPIRNSDAMIDPVILSNAAYDAGLVAEPMSTV
GDALEAIKAVLDPEGLPPRILVGGSLYLVGDVLSDNGTPPK

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=369, Percent_Identity=28.1842818428184, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI66932984, Length=368, Percent_Identity=27.9891304347826, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1788654, Length=373, Percent_Identity=31.9034852546917, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17553150, Length=446, Percent_Identity=28.0269058295964, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=443, Percent_Identity=27.313769751693, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=443, Percent_Identity=27.313769751693, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=433, Percent_Identity=29.0993071593533, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=326, Percent_Identity=26.3803680981595, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641571, Length=318, Percent_Identity=27.9874213836478, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=30.8988764044944, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47391; Mature: 47260

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00018 EF_HAND_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAQEIDKLMGLHPKGFDLSLDRITRLLDVLGNPHRKLPPVIHVAGTNGKGSVTAFCR
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
ALLEAGGYSAHVHTSPHLVNWHERYRIGVAGGRGQLVDDAVFAEALRRVADANAGQHITV
HHHHCCCCEEEEECCCCEECCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEHH
FEILTAVTFILFSEQPADAAIIEVGLGGRFDATNVITDPGVSVIMPISLDHQPYLGDRVE
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEE
LIAAEKAGIMKPGLPVVIGHQEYDAALDVLMSTAERLHCPSAVFGQDFMAHEEYGRLVYQ
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
DEFGLADLPLPRLPGRHQYANAAAAIRAVKAAGFTVTETMMEKAMNSVEWPGRLQRLGEG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
RLLQHAPAGAEIWIDGGHNPGAGEVIAEAMANFEERQSRPLFLIIGMINTKDPVGYFKAF
CEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
AGLVEKVFCVPIRNSDAMIDPVILSNAAYDAGLVAEPMSTVGDALEAIKAVLDPEGLPPR
HHHHHHEEEEEECCCCCEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
ILVGGSLYLVGDVLSDNGTPPK
EEECCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SEAAQEIDKLMGLHPKGFDLSLDRITRLLDVLGNPHRKLPPVIHVAGTNGKGSVTAFCR
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
ALLEAGGYSAHVHTSPHLVNWHERYRIGVAGGRGQLVDDAVFAEALRRVADANAGQHITV
HHHHCCCCEEEEECCCCEECCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEHH
FEILTAVTFILFSEQPADAAIIEVGLGGRFDATNVITDPGVSVIMPISLDHQPYLGDRVE
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEE
LIAAEKAGIMKPGLPVVIGHQEYDAALDVLMSTAERLHCPSAVFGQDFMAHEEYGRLVYQ
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
DEFGLADLPLPRLPGRHQYANAAAAIRAVKAAGFTVTETMMEKAMNSVEWPGRLQRLGEG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
RLLQHAPAGAEIWIDGGHNPGAGEVIAEAMANFEERQSRPLFLIIGMINTKDPVGYFKAF
CEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
AGLVEKVFCVPIRNSDAMIDPVILSNAAYDAGLVAEPMSTVGDALEAIKAVLDPEGLPPR
HHHHHHEEEEEECCCCCEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
ILVGGSLYLVGDVLSDNGTPPK
EEECCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]