Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 209551541

GI number: 209551541

Start: 4124898

End: 4126508

Strand: Direct

Name: pckA [H]

Synonym: Rleg2_3970

Alternate gene names: 209551541

Gene position: 4124898-4126508 (Clockwise)

Preceding gene: 209551528

Following gene: 209551542

Centisome position: 90.9

GC content: 63.19

Gene sequence:

>1611_bases
ATGGAAATGTTCGGAGTTCATAACCCGGCAACAGAGCTGGCAACGGTTGGATTGGGCGGCGCAGCCAGCGTTCGCTACAA
CTTTTCCGCCGCCGCGCTCTATGAAGAATCGATCCGCCGGGGCGAAGCCGAACTGACCGCTCAGGGTGCGCTGCGGGCTA
TCACCGGCCAGCACACCGGCCGTTCGCCGCGTGACAAGTTCGTCGTACGCGACAGCAATACCGATGGCGAAATCTGGTGG
GACAACAACAAGCCGCTTTCGCCGGAACATTTCGCGCTGCTGCGCGCGGATATGCTGGCGCACGCTGCGGGGAAAGACCT
GTTCGTTCAGGATCTCATCGGCGGTGCCGAGGAAGGCCACGCTCTGCCGACCCGTGTCGTCACCGAATTTGCCTGGCACT
CGCTGTTCATCCGCAATCTGCTGATTCGCCCCGACGCCGCAGCGCTGCCGAGCTTTGCGCCGAAGCTGACGATCATCGAT
CTGCCGAGCTTCAAGGCCGATCCGGCCCGCCACGGCTGCCGCTCGGAAACGGTGATCGCCTGCGACCTGACCAATGGTCT
CGTCCTCATCGGCGGCACCTCCTATGCCGGTGAAATGAAAAAGTCGGTCTTTACCGTTCTCAACTACCTGCTGCCCGCCA
AGGGCGTGATGCCGATGCACTGCTCGGCCAATGTCGGCCCCAACGGTGACGCCGCAGTGTTCTTCGGCCTTTCCGGCACC
GGCAAGACGACGCTGTCGGCCGACCCGGCCCGCACGCTGATCGGCGATGACGAACACGGCTGGAGCGAAAACGGCATCTT
CAACTTCGAAGGCGGCTGCTACGCCAAGACCATCCGCCTGTCGGCCGAAGCCGAGCCGGAAATCTATGCGACGACCCAGC
GCTTCGGCACCGTGCTCGAAAACGTCGTGCTGAACGAGCGTCGCGAGCCGAATTTCGATGACGGCTCGCTGACCGAGAAC
ACCCGCTGCGCCTATCCGATGGATTTCATTCCGAACGCTTCAGAAACCGGCCGGGCCGGGCATCCGAAGACGATCATCAT
GCTGACCGCCGATGCCTTCGGCGTCATGCCGCCGATCGCCCGTTTGACGCCCGATCAGGCGATGTATCACTTCCTCTCCG
GCTACACCGCCAAGGTGGCCGGCACCGAAAAGGGTGTCGTCGAGCCGGAAGCAACCTTCTCCACCTGCTTCGGCGCGCCT
TTCATGCCGCGGCATCCGGCTGAATACGGCAATCTGCTCAAGGAACTGATCAGCCGCCACGGCGTCGAATGCTGGCTGGT
GAATACCGGTTGGACCGGCGGCGCCTACGGCACTGGCAAGCGCATGCCGATCAAAGCGACACGCGCGCTGCTCGCAGCCG
CCCTGACCGGCGAACTCGGCCAGGTCGAATTCCGTGCGGATACCAATTTCGGCTTCGCCGTGCCGGTCTCCGTCAACGGC
GTCGACAGCAGCATTCTCGACCCGCGCTCGACCTGGGCCGACAAGGCAGCCTATGACGCACAGGCCGAAAAGCTGGTCTC
GATGTTCATCGCCAACTTCGCGAAATTCGAAGGTCACGTCGATGGCGGCGTCCGCGACGCGGCCCCAGGCGTGAAGGTCG
CGGCCGAATAA

Upstream 100 bases:

>100_bases
ACTATATTTTTTCAAGTTTCGTCTCTTGCGTTTTAAAATTTCTCCCCGTATTTTCGCGATTAGTTTTAACGGCAACGAGC
CTGAGCGAAGGGAACTAGCC

Downstream 100 bases:

>100_bases
AACGAATAATCTTTGACTCACGTGAAACCCGGCATCGCAGGATTGCCGGGTTTTTCGGTTGACGGCCGATATTTTCAACG
GGCTGCCGATGTGAGATAGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MEMFGVHNPATELATVGLGGAASVRYNFSAAALYEESIRRGEAELTAQGALRAITGQHTGRSPRDKFVVRDSNTDGEIWW
DNNKPLSPEHFALLRADMLAHAAGKDLFVQDLIGGAEEGHALPTRVVTEFAWHSLFIRNLLIRPDAAALPSFAPKLTIID
LPSFKADPARHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPAKGVMPMHCSANVGPNGDAAVFFGLSGT
GKTTLSADPARTLIGDDEHGWSENGIFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLNERREPNFDDGSLTEN
TRCAYPMDFIPNASETGRAGHPKTIIMLTADAFGVMPPIARLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAP
FMPRHPAEYGNLLKELISRHGVECWLVNTGWTGGAYGTGKRMPIKATRALLAAALTGELGQVEFRADTNFGFAVPVSVNG
VDSSILDPRSTWADKAAYDAQAEKLVSMFIANFAKFEGHVDGGVRDAAPGVKVAAE

Sequences:

>Translated_536_residues
MEMFGVHNPATELATVGLGGAASVRYNFSAAALYEESIRRGEAELTAQGALRAITGQHTGRSPRDKFVVRDSNTDGEIWW
DNNKPLSPEHFALLRADMLAHAAGKDLFVQDLIGGAEEGHALPTRVVTEFAWHSLFIRNLLIRPDAAALPSFAPKLTIID
LPSFKADPARHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPAKGVMPMHCSANVGPNGDAAVFFGLSGT
GKTTLSADPARTLIGDDEHGWSENGIFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLNERREPNFDDGSLTEN
TRCAYPMDFIPNASETGRAGHPKTIIMLTADAFGVMPPIARLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAP
FMPRHPAEYGNLLKELISRHGVECWLVNTGWTGGAYGTGKRMPIKATRALLAAALTGELGQVEFRADTNFGFAVPVSVNG
VDSSILDPRSTWADKAAYDAQAEKLVSMFIANFAKFEGHVDGGVRDAAPGVKVAAE
>Mature_536_residues
MEMFGVHNPATELATVGLGGAASVRYNFSAAALYEESIRRGEAELTAQGALRAITGQHTGRSPRDKFVVRDSNTDGEIWW
DNNKPLSPEHFALLRADMLAHAAGKDLFVQDLIGGAEEGHALPTRVVTEFAWHSLFIRNLLIRPDAAALPSFAPKLTIID
LPSFKADPARHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPAKGVMPMHCSANVGPNGDAAVFFGLSGT
GKTTLSADPARTLIGDDEHGWSENGIFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLNERREPNFDDGSLTEN
TRCAYPMDFIPNASETGRAGHPKTIIMLTADAFGVMPPIARLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAP
FMPRHPAEYGNLLKELISRHGVECWLVNTGWTGGAYGTGKRMPIKATRALLAAALTGELGQVEFRADTNFGFAVPVSVNG
VDSSILDPRSTWADKAAYDAQAEKLVSMFIANFAKFEGHVDGGVRDAAPGVKVAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=525, Percent_Identity=48.7619047619048, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322950, Length=516, Percent_Identity=45.1550387596899, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 57522; Mature: 57522

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMFGVHNPATELATVGLGGAASVRYNFSAAALYEESIRRGEAELTAQGALRAITGQHTG
CCCCCCCCCHHHHHEECCCCCEEEEECCHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCCC
RSPRDKFVVRDSNTDGEIWWDNNKPLSPEHFALLRADMLAHAAGKDLFVQDLIGGAEEGH
CCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
ALPTRVVTEFAWHSLFIRNLLIRPDAAALPSFAPKLTIIDLPSFKADPARHGCRSETVIA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEE
CDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPAKGVMPMHCSANVGPNGDAAVFFGLSGT
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCC
GKTTLSADPARTLIGDDEHGWSENGIFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLE
CCCEECCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLNERREPNFDDGSLTENTRCAYPMDFIPNASETGRAGHPKTIIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAPFMPRHPAEYGNLLKELISRH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GVECWLVNTGWTGGAYGTGKRMPIKATRALLAAALTGELGQVEFRADTNFGFAVPVSVNG
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECC
VDSSILDPRSTWADKAAYDAQAEKLVSMFIANFAKFEGHVDGGVRDAAPGVKVAAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MEMFGVHNPATELATVGLGGAASVRYNFSAAALYEESIRRGEAELTAQGALRAITGQHTG
CCCCCCCCCHHHHHEECCCCCEEEEECCHHHHHHHHHHHCCCHHEEHHHHHHHHCCCCCC
RSPRDKFVVRDSNTDGEIWWDNNKPLSPEHFALLRADMLAHAAGKDLFVQDLIGGAEEGH
CCCCCCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
ALPTRVVTEFAWHSLFIRNLLIRPDAAALPSFAPKLTIIDLPSFKADPARHGCRSETVIA
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEE
CDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPAKGVMPMHCSANVGPNGDAAVFFGLSGT
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCC
GKTTLSADPARTLIGDDEHGWSENGIFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLE
CCCEECCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLNERREPNFDDGSLTENTRCAYPMDFIPNASETGRAGHPKTIIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RLTPDQAMYHFLSGYTAKVAGTEKGVVEPEATFSTCFGAPFMPRHPAEYGNLLKELISRH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
GVECWLVNTGWTGGAYGTGKRMPIKATRALLAAALTGELGQVEFRADTNFGFAVPVSVNG
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECC
VDSSILDPRSTWADKAAYDAQAEKLVSMFIANFAKFEGHVDGGVRDAAPGVKVAAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA