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The map label for this gene is trpA [H]

Identifier: 209551526

GI number: 209551526

Start: 4103500

End: 4104339

Strand: Direct

Name: trpA [H]

Synonym: Rleg2_3955

Alternate gene names: 209551526

Gene position: 4103500-4104339 (Clockwise)

Preceding gene: 209551525

Following gene: 209551527

Centisome position: 90.43

GC content: 61.55

Gene sequence:

>840_bases
ATGACCGCACGCATGGACAAACGCTTTGCCGAGCTGAAGGCCGAGGGCCGTCCGGCGCTTGTGACCTATTTCATGGGCGG
CGATCCTGATTACGATACCTCGCTCGGCATCATGAAGGCGCTGCCGGAAGCAGGCTCCGACATCATCGAACTCGGCATGC
CCTTTTCCGATCCGATGGCCGACGGACCGGCGATCCAGCTCGCTGGTCAGCGCGCGCTGAAAGGCGGTCAGACGCTGAAG
AAGACGCTGCAGCTGGCGGCCGATTTCCGCAAAACCGATGACGCGACGCCGATCGTGATGATGGGCTATTACAACCCGAT
CTATATTTATGGCGTCGAAAAATTCCTCGACGACGCGATTGCTGCCGGCATCGACGGTCTGATCGTCGTCGATCTGCCGC
CTGAGATGGATGACGAGCTCTGCATTCCGGCGATCCGCAAAGGCATCAATTTCATCCGCCTGGCGACGCCGACGACGGAT
GAGAAGCGCCTGCCGAAGGTTTTGAAGAACACCTCCGGCTTCGTCTATTATGTCTCGATGAACGGCATCACCGGTTCGGC
GCTGCCGGATCCGTCGCTGGTTTCGGGCGCGGTCGAACGCATCAAGCAGCACACGCAACTGCCCGTCTGCGTCGGCTTCG
GCGTCAAGACGGCGGAACATGCAAAGGTCATCGGCGGTTCGGCCGATGGCGTCGTCGTCGGCACCGCCATCGTCAATCAG
GTCGCGACCAGCCTGACGAGTGACGGCAAGGCGACGGCGGACACGGTTCAGGCCGTTGCCACGCTGGTGCGCGGCCTTTC
CACGGGAACACGTTCGGCGCGCCTTGTTGCTGCCGAATAG

Upstream 100 bases:

>100_bases
GAAGATGGGCAAGGACGAGATCATCCTGATGAACCTCTCCGGCCGCGGCGACAAGGATATCTTCACCGTCGGCAAGATTC
TGGGAATGGGACTGTAAGAC

Downstream 100 bases:

>100_bases
TTTCCCCACATTGGCGTCAAAGCATGTCGCGCAAAACTGTGCAGCGGTTTAGCGGGAACACAAAGCCGGCAAGCGATCTT
GAAAGATCGTGACGCGCTTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTARMDKRFAELKAEGRPALVTYFMGGDPDYDTSLGIMKALPEAGSDIIELGMPFSDPMADGPAIQLAGQRALKGGQTLK
KTLQLAADFRKTDDATPIVMMGYYNPIYIYGVEKFLDDAIAAGIDGLIVVDLPPEMDDELCIPAIRKGINFIRLATPTTD
EKRLPKVLKNTSGFVYYVSMNGITGSALPDPSLVSGAVERIKQHTQLPVCVGFGVKTAEHAKVIGGSADGVVVGTAIVNQ
VATSLTSDGKATADTVQAVATLVRGLSTGTRSARLVAAE

Sequences:

>Translated_279_residues
MTARMDKRFAELKAEGRPALVTYFMGGDPDYDTSLGIMKALPEAGSDIIELGMPFSDPMADGPAIQLAGQRALKGGQTLK
KTLQLAADFRKTDDATPIVMMGYYNPIYIYGVEKFLDDAIAAGIDGLIVVDLPPEMDDELCIPAIRKGINFIRLATPTTD
EKRLPKVLKNTSGFVYYVSMNGITGSALPDPSLVSGAVERIKQHTQLPVCVGFGVKTAEHAKVIGGSADGVVVGTAIVNQ
VATSLTSDGKATADTVQAVATLVRGLSTGTRSARLVAAE
>Mature_278_residues
TARMDKRFAELKAEGRPALVTYFMGGDPDYDTSLGIMKALPEAGSDIIELGMPFSDPMADGPAIQLAGQRALKGGQTLKK
TLQLAADFRKTDDATPIVMMGYYNPIYIYGVEKFLDDAIAAGIDGLIVVDLPPEMDDELCIPAIRKGINFIRLATPTTDE
KRLPKVLKNTSGFVYYVSMNGITGSALPDPSLVSGAVERIKQHTQLPVCVGFGVKTAEHAKVIGGSADGVVVGTAIVNQV
ATSLTSDGKATADTVQAVATLVRGLSTGTRSARLVAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=274, Percent_Identity=31.021897810219, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=43.0962343096234, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29466; Mature: 29335

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARMDKRFAELKAEGRPALVTYFMGGDPDYDTSLGIMKALPEAGSDIIELGMPFSDPMA
CCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCC
DGPAIQLAGQRALKGGQTLKKTLQLAADFRKTDDATPIVMMGYYNPIYIYGVEKFLDDAI
CCCEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHH
AAGIDGLIVVDLPPEMDDELCIPAIRKGINFIRLATPTTDEKRLPKVLKNTSGFVYYVSM
HCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEE
NGITGSALPDPSLVSGAVERIKQHTQLPVCVGFGVKTAEHAKVIGGSADGVVVGTAIVNQ
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEECCCCCCEEEHHHHHHH
VATSLTSDGKATADTVQAVATLVRGLSTGTRSARLVAAE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
TARMDKRFAELKAEGRPALVTYFMGGDPDYDTSLGIMKALPEAGSDIIELGMPFSDPMA
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCC
DGPAIQLAGQRALKGGQTLKKTLQLAADFRKTDDATPIVMMGYYNPIYIYGVEKFLDDAI
CCCEEEECCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHH
AAGIDGLIVVDLPPEMDDELCIPAIRKGINFIRLATPTTDEKRLPKVLKNTSGFVYYVSM
HCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEE
NGITGSALPDPSLVSGAVERIKQHTQLPVCVGFGVKTAEHAKVIGGSADGVVVGTAIVNQ
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEECCCCCCEEEHHHHHHH
VATSLTSDGKATADTVQAVATLVRGLSTGTRSARLVAAE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA