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The map label for this gene is ilvA [H]

Identifier: 209551470

GI number: 209551470

Start: 4039322

End: 4040254

Strand: Reverse

Name: ilvA [H]

Synonym: Rleg2_3899

Alternate gene names: 209551470

Gene position: 4040254-4039322 (Counterclockwise)

Preceding gene: 209551473

Following gene: 209551466

Centisome position: 89.03

GC content: 62.81

Gene sequence:

>933_bases
ATGCCTTTCGCACCCCTCCACCTCGACACGCCCCTGGTTCAGACAGCGCCTGGCTACAGTGCCAGCGGCAAGCTGCTCTG
GCTGAAGCTCGATGCGCTGCAGCCTTCCGGCAGCTTCAAGCTGCGTGGCGTCGGACGGCTTTGCCAGCACGAGGTGGAGA
GGGGCGCGCGCGAAATCTTCTGTGCGTCCGGCGGCAATGCCGGCATTGCCGCGGCCTATGCCGGCCGGGCGCTCGGCGTG
CCGGTCACCATCGTCGTGCCGGAGACGACGACCGCCGATGTCCGGCAGACGATCGCCGCAACCGGGGCGAATGTCCTCGT
CCATGGGTCTGTCTTCGATGAAGCCAATGCGCATGCGGTCGAACTCGCAGAGAGCCGCAAGGCGAGTTATGTGCATCCCT
TCGACCATCCGCTTCTGTGGGATGGTCATGCGACACTGATCGACGAGGTGGTGGTGAAGGGTGCAAAATTCGATTGCGTT
ATCACCAGCGTCGGCGGCGGCGGGCTGCTTGCCGGCATTGTCGAGGGGTTGAAGCGCAATGGGCTTGCCGATGTGCCGGT
GATCGCCGTTGAAACCGAGGGTGCGGCTTCGCTCCATGAAAGCCTCAAGGCAAACGAGCGCATTACGCTCCCCGCCATCA
GCTCGATTGCCACTTCGCTCGGGGCGCGGCAGGTGGCACAGCATGTCTTTGACCTGCCGAAACAGCATCCGATCGAAAGC
GTTCTCGTCAGCGACGCCGATGCGGTTGCTGCCTGCCTGAAATTTGCCGATGCGCAGCGCATTCTGGTCGAGCCGGCCTG
CGGTGCAGCACTTGCCGTTGCCGATGTGCATGCCGGGCTGCTTGCCCGCTTTGACAATCCGCTAATCGAAGTCTGCGGCG
GCATCGGCGTATCGCTCGAAAAGCTGAGACTCTGGAAAGAGAAGTTTCTCTGA

Upstream 100 bases:

>100_bases
AGGTGAATCGGTACGCTTCGCTCTCAGTCGTGATGATTGCTCCTGCCCCTGTAAACGAGCTAGAACAGCGTCCTCCCTTT
TATGTCCCAGGATGTTTGCC

Downstream 100 bases:

>100_bases
CCTGATATCAAAGAAAAAGCCGGGCGAAGGCCCGGCTTGATCGAGAATTTGGAGAGCGCTTAAGCTGCCCAGGGGTGCGA
AGCGGCGTTCTTCTTTTCGA

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPFAPLHLDTPLVQTAPGYSASGKLLWLKLDALQPSGSFKLRGVGRLCQHEVERGAREIFCASGGNAGIAAAYAGRALGV
PVTIVVPETTTADVRQTIAATGANVLVHGSVFDEANAHAVELAESRKASYVHPFDHPLLWDGHATLIDEVVVKGAKFDCV
ITSVGGGGLLAGIVEGLKRNGLADVPVIAVETEGAASLHESLKANERITLPAISSIATSLGARQVAQHVFDLPKQHPIES
VLVSDADAVAACLKFADAQRILVEPACGAALAVADVHAGLLARFDNPLIEVCGGIGVSLEKLRLWKEKFL

Sequences:

>Translated_310_residues
MPFAPLHLDTPLVQTAPGYSASGKLLWLKLDALQPSGSFKLRGVGRLCQHEVERGAREIFCASGGNAGIAAAYAGRALGV
PVTIVVPETTTADVRQTIAATGANVLVHGSVFDEANAHAVELAESRKASYVHPFDHPLLWDGHATLIDEVVVKGAKFDCV
ITSVGGGGLLAGIVEGLKRNGLADVPVIAVETEGAASLHESLKANERITLPAISSIATSLGARQVAQHVFDLPKQHPIES
VLVSDADAVAACLKFADAQRILVEPACGAALAVADVHAGLLARFDNPLIEVCGGIGVSLEKLRLWKEKFL
>Mature_309_residues
PFAPLHLDTPLVQTAPGYSASGKLLWLKLDALQPSGSFKLRGVGRLCQHEVERGAREIFCASGGNAGIAAAYAGRALGVP
VTIVVPETTTADVRQTIAATGANVLVHGSVFDEANAHAVELAESRKASYVHPFDHPLLWDGHATLIDEVVVKGAKFDCVI
TSVGGGGLLAGIVEGLKRNGLADVPVIAVETEGAASLHESLKANERITLPAISSIATSLGARQVAQHVFDLPKQHPIESV
LVSDADAVAACLKFADAQRILVEPACGAALAVADVHAGLLARFDNPLIEVCGGIGVSLEKLRLWKEKFL

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI33469958, Length=315, Percent_Identity=45.0793650793651, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI19923959, Length=313, Percent_Identity=44.0894568690096, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI11345492, Length=276, Percent_Identity=28.9855072463768, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1790207, Length=303, Percent_Identity=33.003300330033, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17508781, Length=277, Percent_Identity=28.5198555956679, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71991565, Length=264, Percent_Identity=27.6515151515151, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537387, Length=241, Percent_Identity=26.9709543568465, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319788, Length=287, Percent_Identity=39.7212543554007, Blast_Score=203, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322024, Length=199, Percent_Identity=38.6934673366834, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320930, Length=312, Percent_Identity=31.7307692307692, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322023, Length=116, Percent_Identity=36.2068965517241, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322631, Length=261, Percent_Identity=23.7547892720307, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355833, Length=243, Percent_Identity=30.0411522633745, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 32373; Mature: 32241

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFAPLHLDTPLVQTAPGYSASGKLLWLKLDALQPSGSFKLRGVGRLCQHEVERGAREIF
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEECHHHHHHHHHHHCCHHEEE
CASGGNAGIAAAYAGRALGVPVTIVVPETTTADVRQTIAATGANVLVHGSVFDEANAHAV
EECCCCCCEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
ELAESRKASYVHPFDHPLLWDGHATLIDEVVVKGAKFDCVITSVGGGGLLAGIVEGLKRN
HHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHC
GLADVPVIAVETEGAASLHESLKANERITLPAISSIATSLGARQVAQHVFDLPKQHPIES
CCCCCCEEEEECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VLVSDADAVAACLKFADAQRILVEPACGAALAVADVHAGLLARFDNPLIEVCGGIGVSLE
HHHCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHH
KLRLWKEKFL
HHHHHHHHCC
>Mature Secondary Structure 
PFAPLHLDTPLVQTAPGYSASGKLLWLKLDALQPSGSFKLRGVGRLCQHEVERGAREIF
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEECHHHHHHHHHHHCCHHEEE
CASGGNAGIAAAYAGRALGVPVTIVVPETTTADVRQTIAATGANVLVHGSVFDEANAHAV
EECCCCCCEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHH
ELAESRKASYVHPFDHPLLWDGHATLIDEVVVKGAKFDCVITSVGGGGLLAGIVEGLKRN
HHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHC
GLADVPVIAVETEGAASLHESLKANERITLPAISSIATSLGARQVAQHVFDLPKQHPIES
CCCCCCEEEEECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VLVSDADAVAACLKFADAQRILVEPACGAALAVADVHAGLLARFDNPLIEVCGGIGVSLE
HHHCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHH
KLRLWKEKFL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]