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The map label for this gene is proP [H]

Identifier: 209551473

GI number: 209551473

Start: 4042562

End: 4044448

Strand: Reverse

Name: proP [H]

Synonym: Rleg2_3902

Alternate gene names: 209551473

Gene position: 4044448-4042562 (Counterclockwise)

Preceding gene: 209551474

Following gene: 209551470

Centisome position: 89.13

GC content: 61.79

Gene sequence:

>1887_bases
ATGGCAAATGTCGCAAGCATCGACGGCGCGAAGGCCGGTCCGATGACCAGCGAAGAGAAGAAGGTGATCTTCGCCTCTTC
GCTCGGAACCGTTTTCGAATGGTATGATTTCTATCTCTATGGTTCGCTCGCCACCTATATCGGCGCGACATATTTCACCC
AATATCCCGAGGCTACACGCAACATCTTCACGCTGCTCGCCTTTGCCGCCGGCTTCCTGGTGCGCCCGTTCGGCGCGCTG
GTATTCGGCCGTCTCGGCGACCTGGTTGGCCGGAAGTACACCTTCCTGGTGACGATCATGATCATGGGTCTGTCGACCTT
CCTCGTCGGCATCCTGCCGGGTGCCGCCACCATCGGCATCGCAGCCCCGATCATCCTGATCGCGCTGCGCCTGCTCCAAG
GTCTGGCGTTGGGCGGTGAATATGGCGGTGCGGCAACCTATGTCGCCGAACATGCGCCGAACGGGCGCCGCGGCTACTTC
ACCTCATGGATCCAGACGACGGCGACGCTCGGCCTGTTCCTGTCGCTGATCGTTATCGTCCTGGTTCAATATCTGATGGG
TGCGGCTCAATTCGCCGCCTGGGGCTGGCGTATCCCGTTCCTGGTCTCGGTCGTGCTGCTCGGCATTTCCGTCTGGATCC
GTCTGAAGATGAACGAATCGCCGGCGTTCCAGCGCATGAAGGCGGAAGGCAAGGGCTCCAAGGCGCCGCTGACCGAAGCT
TTCGGGACCTGGAGAAACGCCAAGATTGCGATCATCGCGCTTCTCGGCGCCACCATGGGCCAGGCGGTCGTCTGGTACGG
CGGCCAGTTCTATGCGCTGTTCTTCCTGCAGAACGTGCTGAAGGTAGACCTGCAGTCGGCCAATATCATGGTCGCCATCG
CGCTCTTCCTCGCTACGCCGTTCTTCGTCATCTTCGGCGGTCTCTCCGACAAGATCGGTCGCAAGCCGATCATCATGGCA
GGCCTTCTCATCGCAGCGGTGACGTATAATCCGCTGTTCAAGGCGATGACCTGGACGGCGAACCCGGCGCTTGCCGAAGC
ACAGGCCTCGATCCGGGCAACGGTCACGGCCGACCCGGCGGATTGCAAATTCCAGTTCAATCCGACCGGCACGTCGAAGT
TCACCAGCTCCTGCGATGTGGCGACGGCGTTCCTGACGAAGAACTCGGTGCCTTATGATGTCGTGTCCGGTCCCGCCGGT
CAGCCGGCAACGGTAAAGGTCGGCAACGAGACGATCCCGAGCTTCGACGTCGTTGCCGCCGGCGACAAGGCCAAGGGCAT
GACCGCCGCCTTCGAAAAGGGCGTCAACATCGCGCTCCACGATGCCGGCTATCCCCTGAAGCGCGCCGCCGCCAAGGTGC
CGGATGCCAAGCTCGATGCTTTCATCGCAGCCAATCCGGAACTGTCGCTCAATGCCGATACCGTGCGTGCCGGCGAGAAG
GAAACCATGCCGGCGGCCAAGCTGGTCGAGACCAAGCTGCTGACGGCGGATGAGGCCAACGGCGTCACCGACATGGCGGT
CTACAACGTCGCCAATGGCGGCACCTTCGCCATGACCGCCGATCCCGCCCGCGTCAACTGGATCGGCACGATCGCAATCC
TGTTCGTGCTCGTCCTCTATGTGACGATGGTCTACGGCCCGATCGCCGCTCTGCTGGTCGAGCTCTTCCCGACCCGCATC
CGCTATACCGGCATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGTGGCCTGCTGCCGGCAACGGCCTTCGCGAT
GAGCGCTGCCGCGGGCGATATCTATTACGGTCTCTGGTACCCGATCGTCTTTGCGTCGATCACCCTGGTGATCGGCCTGA
TCTTCCTGCCGGAAACGAAGAACAGAGATATCCACGCCATGGACTGA

Upstream 100 bases:

>100_bases
TGCCGTTTCTGGCACACTCTCGTCAGGCGTGTCGCGGCCAGGGGTCTGCTTTGAGGAGAGTAGGTCGCGCGCGTGCTCAT
CACTCTGTGGAGGACAGACA

Downstream 100 bases:

>100_bases
GCGGCGCTTCAGCGCTTGAGATAATGGCCCGGCGCTTACAAGGCGCCGGGCTTTTTGATGTCGGGAAAGAAGTGCTTCGC
AATCGGCCAGCCATCCGGCG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MANVASIDGAKAGPMTSEEKKVIFASSLGTVFEWYDFYLYGSLATYIGATYFTQYPEATRNIFTLLAFAAGFLVRPFGAL
VFGRLGDLVGRKYTFLVTIMIMGLSTFLVGILPGAATIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPNGRRGYF
TSWIQTTATLGLFLSLIVIVLVQYLMGAAQFAAWGWRIPFLVSVVLLGISVWIRLKMNESPAFQRMKAEGKGSKAPLTEA
FGTWRNAKIAIIALLGATMGQAVVWYGGQFYALFFLQNVLKVDLQSANIMVAIALFLATPFFVIFGGLSDKIGRKPIIMA
GLLIAAVTYNPLFKAMTWTANPALAEAQASIRATVTADPADCKFQFNPTGTSKFTSSCDVATAFLTKNSVPYDVVSGPAG
QPATVKVGNETIPSFDVVAAGDKAKGMTAAFEKGVNIALHDAGYPLKRAAAKVPDAKLDAFIAANPELSLNADTVRAGEK
ETMPAAKLVETKLLTADEANGVTDMAVYNVANGGTFAMTADPARVNWIGTIAILFVLVLYVTMVYGPIAALLVELFPTRI
RYTGMSLPYHIGNGWFGGLLPATAFAMSAAAGDIYYGLWYPIVFASITLVIGLIFLPETKNRDIHAMD

Sequences:

>Translated_628_residues
MANVASIDGAKAGPMTSEEKKVIFASSLGTVFEWYDFYLYGSLATYIGATYFTQYPEATRNIFTLLAFAAGFLVRPFGAL
VFGRLGDLVGRKYTFLVTIMIMGLSTFLVGILPGAATIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPNGRRGYF
TSWIQTTATLGLFLSLIVIVLVQYLMGAAQFAAWGWRIPFLVSVVLLGISVWIRLKMNESPAFQRMKAEGKGSKAPLTEA
FGTWRNAKIAIIALLGATMGQAVVWYGGQFYALFFLQNVLKVDLQSANIMVAIALFLATPFFVIFGGLSDKIGRKPIIMA
GLLIAAVTYNPLFKAMTWTANPALAEAQASIRATVTADPADCKFQFNPTGTSKFTSSCDVATAFLTKNSVPYDVVSGPAG
QPATVKVGNETIPSFDVVAAGDKAKGMTAAFEKGVNIALHDAGYPLKRAAAKVPDAKLDAFIAANPELSLNADTVRAGEK
ETMPAAKLVETKLLTADEANGVTDMAVYNVANGGTFAMTADPARVNWIGTIAILFVLVLYVTMVYGPIAALLVELFPTRI
RYTGMSLPYHIGNGWFGGLLPATAFAMSAAAGDIYYGLWYPIVFASITLVIGLIFLPETKNRDIHAMD
>Mature_627_residues
ANVASIDGAKAGPMTSEEKKVIFASSLGTVFEWYDFYLYGSLATYIGATYFTQYPEATRNIFTLLAFAAGFLVRPFGALV
FGRLGDLVGRKYTFLVTIMIMGLSTFLVGILPGAATIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPNGRRGYFT
SWIQTTATLGLFLSLIVIVLVQYLMGAAQFAAWGWRIPFLVSVVLLGISVWIRLKMNESPAFQRMKAEGKGSKAPLTEAF
GTWRNAKIAIIALLGATMGQAVVWYGGQFYALFFLQNVLKVDLQSANIMVAIALFLATPFFVIFGGLSDKIGRKPIIMAG
LLIAAVTYNPLFKAMTWTANPALAEAQASIRATVTADPADCKFQFNPTGTSKFTSSCDVATAFLTKNSVPYDVVSGPAGQ
PATVKVGNETIPSFDVVAAGDKAKGMTAAFEKGVNIALHDAGYPLKRAAAKVPDAKLDAFIAANPELSLNADTVRAGEKE
TMPAAKLVETKLLTADEANGVTDMAVYNVANGGTFAMTADPARVNWIGTIAILFVLVLYVTMVYGPIAALLVELFPTRIR
YTGMSLPYHIGNGWFGGLLPATAFAMSAAAGDIYYGLWYPIVFASITLVIGLIFLPETKNRDIHAMD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=332, Percent_Identity=34.6385542168675, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788292, Length=334, Percent_Identity=34.4311377245509, Blast_Score=183, Evalue=4e-47,
Organism=Escherichia coli, GI1788942, Length=330, Percent_Identity=34.5454545454545, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789941, Length=367, Percent_Identity=33.2425068119891, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=26.0089686098655, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=24.2902208201893, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 67086; Mature: 66955

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVASIDGAKAGPMTSEEKKVIFASSLGTVFEWYDFYLYGSLATYIGATYFTQYPEATR
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
NIFTLLAFAAGFLVRPFGALVFGRLGDLVGRKYTFLVTIMIMGLSTFLVGILPGAATIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AAPIILIALRLLQGLALGGEYGGAATYVAEHAPNGRRGYFTSWIQTTATLGLFLSLIVIV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVQYLMGAAQFAAWGWRIPFLVSVVLLGISVWIRLKMNESPAFQRMKAEGKGSKAPLTEA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCHHHH
FGTWRNAKIAIIALLGATMGQAVVWYGGQFYALFFLQNVLKVDLQSANIMVAIALFLATP
HCCCCCCCEEEEEEHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FFVIFGGLSDKIGRKPIIMAGLLIAAVTYNPLFKAMTWTANPALAEAQASIRATVTADPA
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCC
DCKFQFNPTGTSKFTSSCDVATAFLTKNSVPYDVVSGPAGQPATVKVGNETIPSFDVVAA
CCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEC
GDKAKGMTAAFEKGVNIALHDAGYPLKRAAAKVPDAKLDAFIAANPELSLNADTVRAGEK
CCCCCCCHHHHHCCCEEEEECCCCCHHHHHHCCCCCHHCEEEECCCCCCCCCHHHCCCCC
ETMPAAKLVETKLLTADEANGVTDMAVYNVANGGTFAMTADPARVNWIGTIAILFVLVLY
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHH
VTMVYGPIAALLVELFPTRIRYTGMSLPYHIGNGWFGGLLPATAFAMSAAAGDIYYGLWY
HHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
PIVFASITLVIGLIFLPETKNRDIHAMD
HHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANVASIDGAKAGPMTSEEKKVIFASSLGTVFEWYDFYLYGSLATYIGATYFTQYPEATR
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
NIFTLLAFAAGFLVRPFGALVFGRLGDLVGRKYTFLVTIMIMGLSTFLVGILPGAATIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AAPIILIALRLLQGLALGGEYGGAATYVAEHAPNGRRGYFTSWIQTTATLGLFLSLIVIV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVQYLMGAAQFAAWGWRIPFLVSVVLLGISVWIRLKMNESPAFQRMKAEGKGSKAPLTEA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCHHHH
FGTWRNAKIAIIALLGATMGQAVVWYGGQFYALFFLQNVLKVDLQSANIMVAIALFLATP
HCCCCCCCEEEEEEHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FFVIFGGLSDKIGRKPIIMAGLLIAAVTYNPLFKAMTWTANPALAEAQASIRATVTADPA
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCC
DCKFQFNPTGTSKFTSSCDVATAFLTKNSVPYDVVSGPAGQPATVKVGNETIPSFDVVAA
CCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEC
GDKAKGMTAAFEKGVNIALHDAGYPLKRAAAKVPDAKLDAFIAANPELSLNADTVRAGEK
CCCCCCCHHHHHCCCEEEEECCCCCHHHHHHCCCCCHHCEEEECCCCCCCCCHHHCCCCC
ETMPAAKLVETKLLTADEANGVTDMAVYNVANGGTFAMTADPARVNWIGTIAILFVLVLY
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHH
VTMVYGPIAALLVELFPTRIRYTGMSLPYHIGNGWFGGLLPATAFAMSAAAGDIYYGLWY
HHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
PIVFASITLVIGLIFLPETKNRDIHAMD
HHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]