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The map label for this gene is mcl2 [H]

Identifier: 209551466

GI number: 209551466

Start: 4036733

End: 4037641

Strand: Reverse

Name: mcl2 [H]

Synonym: Rleg2_3895

Alternate gene names: 209551466

Gene position: 4037641-4036733 (Counterclockwise)

Preceding gene: 209551470

Following gene: 209551465

Centisome position: 88.98

GC content: 61.17

Gene sequence:

>909_bases
ATGAGCCAAACCATCCCTCGCCGTTCGTTAAATCTGCGCCGCTCGGTGCTGAGCGTGCCCGCAATCAATCTCCGGGCGCT
CGAAAAAAGCCATTCGCTTGATTGCGATGCGGTTATTTTCGATCTGGAGGATTCCGTTTCGCCCGAGAAGAAGGAGCAAG
CGCGGGAAAATCTGCGCGCCTTTTTTGCCGGCCCGCCGCTTGAGGGCAAGGAAAGGATCATCCGCATCAATTCTTTGTCA
TCCGAATTCGGGCCGGCGGACCTGGATCTGGTCAAGGCGCTTTTGCCCGATGCCGTTCTCCTTCCCAAGGTCGACGGACC
TCGAGATATCACCGATATGGGCGATCTGCTCGCCGATGCGGATGCGCCGGAGGAATTGCGCATCTGGGCGATGATCGAGA
CGCCGCGCGGCGTGCTGAATGCCGGAGCGATCGCCGAATCCGGCCGCACGCCGGGCTCGCGGCTCGATTGCCTCGTCGTC
GGGCTCAACGATCTGCGCAAGGAAACAGGCGTCCTACCGCAGCCGGGACGAACCTATCTCGTGCCGTGGCTGATGCAGGT
CGTCCTGGCGGTCAGCGCCTACGGGCTCGATGCGATCGACAGCGTCTTCAACGATTTCAGGGACGAGCAGGGTTTCGATG
CCGAATGCCTGCAGGGCCGGGCCATGGGCTTTGCCGGCAAGATGCTGATCCATCCGGCGCAGATCGAGGCCGCCAATCGG
CATTTCGGCCCGGATCCGGCAGCGATTGCGGAAGCGGAGGCGATCATCGCTGCCTTTGCCGATCCGGCTTCCGACGGGCT
GAATGTCATCAATTCAGGCGGGCGGATGATCGAGCGGCTGCATCTTGTCCAAGCCGAAGCTCTGGTTCATAAAGCTCGCC
TGATTTCTGCAAGAAAGCCCGCCCGATGA

Upstream 100 bases:

>100_bases
TTCTTCGGAAAGGAATGTGGTCCTTCTTTTTCCTCTCTGCAAGGCAAGCATGGCTTTTTGTTGTCTTGCCGGGCTTTGCG
AAGCTTGGCACTAATGATCG

Downstream 100 bases:

>100_bases
AACTCTACCGCTTCCTGACCGGTCCCGACGATGCTTCCTTCTGCCACAAGGTCACCGCCGCCCTCAACAAGGGCTGGTCG
CTGGAGGGCTCGCCGACCTA

Product: citrate (pro-3S)-lyase

Products: acetyl-CoA; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSQTIPRRSLNLRRSVLSVPAINLRALEKSHSLDCDAVIFDLEDSVSPEKKEQARENLRAFFAGPPLEGKERIIRINSLS
SEFGPADLDLVKALLPDAVLLPKVDGPRDITDMGDLLADADAPEELRIWAMIETPRGVLNAGAIAESGRTPGSRLDCLVV
GLNDLRKETGVLPQPGRTYLVPWLMQVVLAVSAYGLDAIDSVFNDFRDEQGFDAECLQGRAMGFAGKMLIHPAQIEAANR
HFGPDPAAIAEAEAIIAAFADPASDGLNVINSGGRMIERLHLVQAEALVHKARLISARKPAR

Sequences:

>Translated_302_residues
MSQTIPRRSLNLRRSVLSVPAINLRALEKSHSLDCDAVIFDLEDSVSPEKKEQARENLRAFFAGPPLEGKERIIRINSLS
SEFGPADLDLVKALLPDAVLLPKVDGPRDITDMGDLLADADAPEELRIWAMIETPRGVLNAGAIAESGRTPGSRLDCLVV
GLNDLRKETGVLPQPGRTYLVPWLMQVVLAVSAYGLDAIDSVFNDFRDEQGFDAECLQGRAMGFAGKMLIHPAQIEAANR
HFGPDPAAIAEAEAIIAAFADPASDGLNVINSGGRMIERLHLVQAEALVHKARLISARKPAR
>Mature_301_residues
SQTIPRRSLNLRRSVLSVPAINLRALEKSHSLDCDAVIFDLEDSVSPEKKEQARENLRAFFAGPPLEGKERIIRINSLSS
EFGPADLDLVKALLPDAVLLPKVDGPRDITDMGDLLADADAPEELRIWAMIETPRGVLNAGAIAESGRTPGSRLDCLVVG
LNDLRKETGVLPQPGRTYLVPWLMQVVLAVSAYGLDAIDSVFNDFRDEQGFDAECLQGRAMGFAGKMLIHPAQIEAANRH
FGPDPAAIAEAEAIIAAFADPASDGLNVINSGGRMIERLHLVQAEALVHKARLISARKPAR

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=296, Percent_Identity=33.445945945946, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI87081764, Length=304, Percent_Identity=29.9342105263158, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557458, Length=293, Percent_Identity=29.3515358361775, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.34; 4.1.3.6

Molecular weight: Translated: 32722; Mature: 32591

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTIPRRSLNLRRSVLSVPAINLRALEKSHSLDCDAVIFDLEDSVSPEKKEQARENLRA
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
FFAGPPLEGKERIIRINSLSSEFGPADLDLVKALLPDAVLLPKVDGPRDITDMGDLLADA
HHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCC
DAPEELRIWAMIETPRGVLNAGAIAESGRTPGSRLDCLVVGLNDLRKETGVLPQPGRTYL
CCCCCEEEEEEEECCCCHHHCCCHHHCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCEEH
VPWLMQVVLAVSAYGLDAIDSVFNDFRDEQGFDAECLQGRAMGFAGKMLIHPAQIEAANR
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHEECHHHHHHCCC
HFGPDPAAIAEAEAIIAAFADPASDGLNVINSGGRMIERLHLVQAEALVHKARLISARKP
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AR
CC
>Mature Secondary Structure 
SQTIPRRSLNLRRSVLSVPAINLRALEKSHSLDCDAVIFDLEDSVSPEKKEQARENLRA
CCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
FFAGPPLEGKERIIRINSLSSEFGPADLDLVKALLPDAVLLPKVDGPRDITDMGDLLADA
HHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHCC
DAPEELRIWAMIETPRGVLNAGAIAESGRTPGSRLDCLVVGLNDLRKETGVLPQPGRTYL
CCCCCEEEEEEEECCCCHHHCCCHHHCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCEEH
VPWLMQVVLAVSAYGLDAIDSVFNDFRDEQGFDAECLQGRAMGFAGKMLIHPAQIEAANR
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHEECHHHHHHCCC
HFGPDPAAIAEAEAIIAAFADPASDGLNVINSGGRMIERLHLVQAEALVHKARLISARKP
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-citryl-CoA

Specific reaction: (3S)-citryl-CoA = acetyl-CoA + oxaloacetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA