Click here to switch to the map view.

The map label for this gene is ybiO [H]

Identifier: 209550804

GI number: 209550804

Start: 3315787

End: 3317901

Strand: Reverse

Name: ybiO [H]

Synonym: Rleg2_3228

Alternate gene names: 209550804

Gene position: 3317901-3315787 (Counterclockwise)

Preceding gene: 209550805

Following gene: 209550802

Centisome position: 73.11

GC content: 63.69

Gene sequence:

>2115_bases
ATGGAGCGTGACGCGGCAGGGGTCTTGAAGGCCGCCATATCGGGAATATTGGCACTCATCGTGCTTCTTGGGGGGATCTC
GCCAAGCTTTGCCCAGACATCGCCGGCGCAAGCGCCGGCCGCGGCGGCTCCGCCGGCGCAGGTGCGTGAGATGATGCAGC
TCATGCAGACGCCCGAAGTTAAGCAGTGGATGTCGACCCAGATGGCGACGGCACCTGCGGGCGGTTCCGCGGCTGCGGAT
CAGATGTCGGTGCTGTCTGGCCTGCTGCAGCATGCCCGCCGGCACGTCGCGATGGTCTCGGATGCGGCGATGACGCTGCC
CTTTCAGATCGGCAATGCGTCCTCGCTGTTCCTAGGTGAAATCGCCGCGAACGGCTGGCCGCGCATGGTGGCGCAGTTCC
TGGCCATCTTCCTGCTCGGCGCGATGGTCGAGAGTCTTGCCCGTTATGCCTTCCGCCGCCGCCGTCGTCGACTGGCCGCG
ATGGCCGGCATGCATCTGGCGCCGCGGGTCATACCGGCGCTGATCTTCGCGGCGGCAACGGTGCTTGCGCTTCTGAGCCT
CGGCTGGCCGCCGCTCAGCCAGAGTATCGCGATTGTCTGCGTTGTCGCGCTCATCGTTCAACGCTTCACCATCTGCCTCG
GCGGCGTGCTGGTCGATCTCATCGGGCTGGCGCGGGCCGAGGAAGAACGGCAGGGCATTACCGCTCCCACCATGCCAGGG
CGTGTCGTTGCACTCTTCTGGTACCGCCGCTGCGCGACCTTCGTCATCTATTTCATGCTTGGCTGGGCGGCGATCCAGTC
GATGGAGCCGCTTGGCTATTCCCCGAGCGCGCGGCTCCTCGTCGGCTACATCCTCGGTATCGGCCTGCTGCTGATTGCGA
TCGAAGCGGTCTGGTCGCGGCCGCACAAGGACGAGAAGCGCGGTCACGGGACCTCCTGGGCGCTCACCGTCTATTTTCTG
TTGCTCTGGAGCCTCTGGGTCGCAGGCTTCAACTGGCTGCTCTGGCTCGGCATCTATGTGCTGCTGCTGCCGCGCGTGCT
TGCCGTCTCGACGCTTGCGGTAAAATCGCTGCAGCAGACCGAGGCGAGCTTCCTGGCGACGCGCCGCATTGCGACCGTGC
TTCTCGACCGGGGTGTGCGGGCGCTGATCATTGCCGTTGCCGCCGTCTGGCTCGGGCATATGCTCGGCGTCGGCGCCGAT
ACGATGGCCGCCGGCGAGACGATGGTGGACAAGATCGCCCGCGGCGTCATCGGCGGCATCGTCATCCTGCTTGCCGCCGA
TCTGCTCTGGCATGTCATCAAAGCCTATATCGACGGCAAGCTGCTCGATTCTTCCGTGGATGGCGGAGCAACGGATGAGG
AGAGGGCCAGACGCGCACGGATACAGACGCTGTTGCCGATCTTCCGCAATATCCTGGCCGTCGTCATCGCCGTCATCGCC
GTTTTGATGGTGCTCTCCGGGCTCGGCATCGAGATCGGACCGCTGATCGCCGGCGCCGGCGTCGTTGGCGTCGCGGTCGG
TTTCGGTGCGCAGACGATCGTCAAGGATGTCATCAGCGGCATGTTCTATCTTTGGGACGATGCCTTCCGTATCGGCGAAT
ATATCGAAAGCGGCAGCCACAAGGGCGTGGTCGAGGCTTTCAGCCTGCGCTCGGTGAAGCTCAGGCATCATCGCGGGCCG
CTGACGACGGTGCCGTTCGGCGAACTCGGCGCGGTGAAGAACCTCAACCGTGACTGGACGATCGACAAGATCAGCCTCAA
CGTCAAATACGACACCGATCTCGTCAAGGCGAAGAAGGTGATCAAGCAGATCGGCCAGACGCTGCTCGAAAATCCGGAAT
TCGGCCCTCATATCATCGAGACGCTGAAGATGAAGGGTGTCGAGCAGTTCGGAGAATTCGCGATCGAAATCCGGCTGTCG
ATGATGACGAAGCCCGGCGAGCAATTCGTCATCCGCCGCAATGCGCTGGCGATGATCCGCAACGCCTTCAAGGAAAACGG
CATCGAATTCGCCGTGCCGACTGTGCAGGTGGCGGGTGATCGCGATGCGGAGGTGGATGCTGCCGTGGCGCGCTACGCCG
CCCATGCGCGGGCGAATGGCGAACCGGCGGCATAA

Upstream 100 bases:

>100_bases
CCTACCGCTTGCTGGCGGCAGCGGTCGCGAGTATCCATTGCCCAGAGGGAAATGTCGGGGTGCTTCGTCCAAGCGGCCGA
CACTCCATTGGAGGGCGGTG

Downstream 100 bases:

>100_bases
GCCGCGTCACATCGGGCCGGCCGCTGTAACCAGCAGGCCGCCGATGATCGCCATGTTGGTTTTCCAGGTATTGATCGCGC
TTTCGCGGGCCGTTCCCTGC

Product: MscS Mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MERDAAGVLKAAISGILALIVLLGGISPSFAQTSPAQAPAAAAPPAQVREMMQLMQTPEVKQWMSTQMATAPAGGSAAAD
QMSVLSGLLQHARRHVAMVSDAAMTLPFQIGNASSLFLGEIAANGWPRMVAQFLAIFLLGAMVESLARYAFRRRRRRLAA
MAGMHLAPRVIPALIFAAATVLALLSLGWPPLSQSIAIVCVVALIVQRFTICLGGVLVDLIGLARAEEERQGITAPTMPG
RVVALFWYRRCATFVIYFMLGWAAIQSMEPLGYSPSARLLVGYILGIGLLLIAIEAVWSRPHKDEKRGHGTSWALTVYFL
LLWSLWVAGFNWLLWLGIYVLLLPRVLAVSTLAVKSLQQTEASFLATRRIATVLLDRGVRALIIAVAAVWLGHMLGVGAD
TMAAGETMVDKIARGVIGGIVILLAADLLWHVIKAYIDGKLLDSSVDGGATDEERARRARIQTLLPIFRNILAVVIAVIA
VLMVLSGLGIEIGPLIAGAGVVGVAVGFGAQTIVKDVISGMFYLWDDAFRIGEYIESGSHKGVVEAFSLRSVKLRHHRGP
LTTVPFGELGAVKNLNRDWTIDKISLNVKYDTDLVKAKKVIKQIGQTLLENPEFGPHIIETLKMKGVEQFGEFAIEIRLS
MMTKPGEQFVIRRNALAMIRNAFKENGIEFAVPTVQVAGDRDAEVDAAVARYAAHARANGEPAA

Sequences:

>Translated_704_residues
MERDAAGVLKAAISGILALIVLLGGISPSFAQTSPAQAPAAAAPPAQVREMMQLMQTPEVKQWMSTQMATAPAGGSAAAD
QMSVLSGLLQHARRHVAMVSDAAMTLPFQIGNASSLFLGEIAANGWPRMVAQFLAIFLLGAMVESLARYAFRRRRRRLAA
MAGMHLAPRVIPALIFAAATVLALLSLGWPPLSQSIAIVCVVALIVQRFTICLGGVLVDLIGLARAEEERQGITAPTMPG
RVVALFWYRRCATFVIYFMLGWAAIQSMEPLGYSPSARLLVGYILGIGLLLIAIEAVWSRPHKDEKRGHGTSWALTVYFL
LLWSLWVAGFNWLLWLGIYVLLLPRVLAVSTLAVKSLQQTEASFLATRRIATVLLDRGVRALIIAVAAVWLGHMLGVGAD
TMAAGETMVDKIARGVIGGIVILLAADLLWHVIKAYIDGKLLDSSVDGGATDEERARRARIQTLLPIFRNILAVVIAVIA
VLMVLSGLGIEIGPLIAGAGVVGVAVGFGAQTIVKDVISGMFYLWDDAFRIGEYIESGSHKGVVEAFSLRSVKLRHHRGP
LTTVPFGELGAVKNLNRDWTIDKISLNVKYDTDLVKAKKVIKQIGQTLLENPEFGPHIIETLKMKGVEQFGEFAIEIRLS
MMTKPGEQFVIRRNALAMIRNAFKENGIEFAVPTVQVAGDRDAEVDAAVARYAAHARANGEPAA
>Mature_704_residues
MERDAAGVLKAAISGILALIVLLGGISPSFAQTSPAQAPAAAAPPAQVREMMQLMQTPEVKQWMSTQMATAPAGGSAAAD
QMSVLSGLLQHARRHVAMVSDAAMTLPFQIGNASSLFLGEIAANGWPRMVAQFLAIFLLGAMVESLARYAFRRRRRRLAA
MAGMHLAPRVIPALIFAAATVLALLSLGWPPLSQSIAIVCVVALIVQRFTICLGGVLVDLIGLARAEEERQGITAPTMPG
RVVALFWYRRCATFVIYFMLGWAAIQSMEPLGYSPSARLLVGYILGIGLLLIAIEAVWSRPHKDEKRGHGTSWALTVYFL
LLWSLWVAGFNWLLWLGIYVLLLPRVLAVSTLAVKSLQQTEASFLATRRIATVLLDRGVRALIIAVAAVWLGHMLGVGAD
TMAAGETMVDKIARGVIGGIVILLAADLLWHVIKAYIDGKLLDSSVDGGATDEERARRARIQTLLPIFRNILAVVIAVIA
VLMVLSGLGIEIGPLIAGAGVVGVAVGFGAQTIVKDVISGMFYLWDDAFRIGEYIESGSHKGVVEAFSLRSVKLRHHRGP
LTTVPFGELGAVKNLNRDWTIDKISLNVKYDTDLVKAKKVIKQIGQTLLENPEFGPHIIETLKMKGVEQFGEFAIEIRLS
MMTKPGEQFVIRRNALAMIRNAFKENGIEFAVPTVQVAGDRDAEVDAAVARYAAHARANGEPAA

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI87081787, Length=274, Percent_Identity=32.1167883211679, Blast_Score=119, Evalue=9e-28,
Organism=Escherichia coli, GI1789291, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 75935; Mature: 75935

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDAAGVLKAAISGILALIVLLGGISPSFAQTSPAQAPAAAAPPAQVREMMQLMQTPEV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KQWMSTQMATAPAGGSAAADQMSVLSGLLQHARRHVAMVSDAAMTLPFQIGNASSLFLGE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
IAANGWPRMVAQFLAIFLLGAMVESLARYAFRRRRRRLAAMAGMHLAPRVIPALIFAAAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VLALLSLGWPPLSQSIAIVCVVALIVQRFTICLGGVLVDLIGLARAEEERQGITAPTMPG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RVVALFWYRRCATFVIYFMLGWAAIQSMEPLGYSPSARLLVGYILGIGLLLIAIEAVWSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PHKDEKRGHGTSWALTVYFLLLWSLWVAGFNWLLWLGIYVLLLPRVLAVSTLAVKSLQQT
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASFLATRRIATVLLDRGVRALIIAVAAVWLGHMLGVGADTMAAGETMVDKIARGVIGGI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHH
VILLAADLLWHVIKAYIDGKLLDSSVDGGATDEERARRARIQTLLPIFRNILAVVIAVIA
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLMVLSGLGIEIGPLIAGAGVVGVAVGFGAQTIVKDVISGMFYLWDDAFRIGEYIESGSH
HHHHHHCCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGVVEAFSLRSVKLRHHRGPLTTVPFGELGAVKNLNRDWTIDKISLNVKYDTDLVKAKKV
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHHH
IKQIGQTLLENPEFGPHIIETLKMKGVEQFGEFAIEIRLSMMTKPGEQFVIRRNALAMIR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NAFKENGIEFAVPTVQVAGDRDAEVDAAVARYAAHARANGEPAA
HHHHHCCCEEEECEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERDAAGVLKAAISGILALIVLLGGISPSFAQTSPAQAPAAAAPPAQVREMMQLMQTPEV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
KQWMSTQMATAPAGGSAAADQMSVLSGLLQHARRHVAMVSDAAMTLPFQIGNASSLFLGE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
IAANGWPRMVAQFLAIFLLGAMVESLARYAFRRRRRRLAAMAGMHLAPRVIPALIFAAAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VLALLSLGWPPLSQSIAIVCVVALIVQRFTICLGGVLVDLIGLARAEEERQGITAPTMPG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
RVVALFWYRRCATFVIYFMLGWAAIQSMEPLGYSPSARLLVGYILGIGLLLIAIEAVWSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PHKDEKRGHGTSWALTVYFLLLWSLWVAGFNWLLWLGIYVLLLPRVLAVSTLAVKSLQQT
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EASFLATRRIATVLLDRGVRALIIAVAAVWLGHMLGVGADTMAAGETMVDKIARGVIGGI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHH
VILLAADLLWHVIKAYIDGKLLDSSVDGGATDEERARRARIQTLLPIFRNILAVVIAVIA
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLMVLSGLGIEIGPLIAGAGVVGVAVGFGAQTIVKDVISGMFYLWDDAFRIGEYIESGSH
HHHHHHCCCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGVVEAFSLRSVKLRHHRGPLTTVPFGELGAVKNLNRDWTIDKISLNVKYDTDLVKAKKV
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHHH
IKQIGQTLLENPEFGPHIIETLKMKGVEQFGEFAIEIRLSMMTKPGEQFVIRRNALAMIR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NAFKENGIEFAVPTVQVAGDRDAEVDAAVARYAAHARANGEPAA
HHHHHCCCEEEECEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]