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The map label for this gene is mprF [H]

Identifier: 209550802

GI number: 209550802

Start: 3312563

End: 3315166

Strand: Reverse

Name: mprF [H]

Synonym: Rleg2_3226

Alternate gene names: 209550802

Gene position: 3315166-3312563 (Counterclockwise)

Preceding gene: 209550804

Following gene: 209550801

Centisome position: 73.05

GC content: 62.48

Gene sequence:

>2604_bases
ATGTCGGGTCACGACAATTTGAAAGAGATCGAAGACGCGGAGGGATTTTCTTTTCGCGGTTTATTCAGACGTTACAGAAC
ACCGCTGACGGCAGCGGCGACACTCGTGGTCTTCTGCCTCGTCGGCTATGCGATCATGCAGCTCACCAACGAAGTGCGCT
ATGACGATGTCGTCGATGCGCTGGCGGCGACCAGGCCGAGCGCCATCCTGCTTGCCCTGTTCTTTACCGCACTCAGTTTC
CTCTCGCTGGTTTTCTACGATCTCAACGCCATCGAATATATCGGCAAGAAGCTACCCTTTCCGCATGTGGCGCTGACGGC
GTTCAGCGCCTATGCCGTCGGCAATACCGCCGGTTTCGGCGCGCTTTCGGGTGGCGCGATCCGCTACCGCGCCTATACGC
GCCTCGGGCTTTCGCCCGAAGATATCGGACGCATCATCGCCTTCGTCACGCTTTCCTTCGGTCTCGGGCTTGCGGGAGTG
GGAGCGATCGCCCTGATCATCATCGCAGACGAGATCGGCCCGCTGATCGGCGTCAGCCCCTTCCTGCTGCGGTTGATCGC
CGGCTCGATCGTCGCCATTCTGGGCGCCGTGATGATCATCGGCCGGGATGGGCGCGTGCTCGATCTCGGCCCTGTCGCAA
TCCGCCTGCCGGATTCGCGCACCTGGTCGCGGCAGTTCCTCGTCACCGCCTTCGATATCGCCGCTTCGGCGTCCGTGCTT
TACGTGCTGCTGCCGCAGACGGCCATCGGCTGGCCGGTCTTCCTGGCCGTCTATGCGATCGCCGTCGGTCTCGGCGTACT
CAGCCACGTTCCGGCCGGGCTCGGCGTGTTCGAAACCGTGATCATCGCCTCGCTCGGCAGCGCGGTGAACATCGATGCGG
TGCTCGGATCGCTGGTGCTCTACCGTCTGATCTATCATGTGCTGCCGCTCTTGATCGCCGTGCTTGCGGTTTCGGCGACG
GAACTGCGCCGTTTCGTCGACCATCCGGCCGCCTCCAGCGTCCGGCGCATCGGCGGAAGGCTGATGCCGCAGCTATTGTC
GGCGCTTGCACTGCTGCTCGGCGTCATGCTGATCTTTTCGAGCGTCACACCGACACCGGACCAGAACCTCGAATTCCTTT
CCAACTATCTGCCGCTGCCGATGGTGGAAGGGGCGCATTTCCTTTCCAGCCTGCTCGGGCTCGCGCTCGTCGTCGCTGCG
CGCGGGCTCGGCCAGCGGCTCGACGGCGCTTGGTGGGTGGCGGTATTTTCGGCCCTTGCCGCACTGACTTTGTCGCTCTT
GAAAGCGATCGCGCTCGTCGAAGCCGCCTTTCTCGCCTTCCTCATCTTCGGGCTCTTCGTCAGCCGGCGGCTCTTTACCC
GCCATGCCTCGCTGCTCAATCAGGCGCTGACGGCGTCCTGGCTGATGGCGATCGCGGTCATCGTCGTCGGTGCCGTCGTC
ATCCTGCTCTTCGTCTATCGCGATGTCGAATACAGCAACCAGCTCTGGTGGCAGTTCGAGTTTACCGCCGAAGCGCCGCG
TGGCTTACGCGCCGTGCTCGGCATCACCATCATCTCGTCGGCGATCGCCATCTTCAGCCTGCTCCGGCCGGCCACCTTCC
GGCCGGAACCGGCGACGGAGGAGGCACTGGCGCGTGCCGTCGAGATCGTCGGCAAGCAGGGCAATGCCGATGCCAATCTG
GTGCGCATGGGCGACAAGAGCATCATGTTCTCGGAAAAGGGTGACGCCTTCATCATGTACGGCCGGCAGGGCCGGTCCTG
GATCGCGCTGTTCGACCCGGTCGGCGAACATCGCGCCGGGCAGGAGCTCGTCTGGCGTTTCGTCGAAGCAGCGCGCGCCG
CCGGCTGCCGCGCCGTGTTCTATCAGATCTCGCCGTCGCTGCTTTCCCATTGCGCCGATGCCGGCCTTCGCGCCTTCAAG
CTCGGCGAACTGGCAGTGACCGATCTTAGAACCTTCGAGATGAAGGGCGGCAAATGGGCGAACCTTCGCCAGACCGCGAG
CCGCGCCCAGCGCGACGGGCTGGAATTTGCCGTTGTCGAACCTGAGGACGTGCCCTCGGTCATCGATGATCTCTCCGCTG
TCTCCACGGCCTGGCTCGAGCATCACAATGCCAAGGAAAAGGGCTTTTCACTCGGCGCCTTCGATCCCGATTACGTCTCT
TCCCAGCCGGTCGGTATCCTGAAAAAGGACGGCAGGATCGTTGCCTTCGCCAATATCCTCGTCACCGAATCCAGGCAGGA
GGGCACGATCGATCTCATGCGCTTCTCGCCGGACGCGCCGAAGGGCTCGATGGACTTTCTCTTCGTGCAGATCATGGAAT
ATCTGCGCGGCCAGGGTTTCACCCACTTCAATCTCGGCATGGCGCCTCTCTCCGGCATGTCGAAACGCGAGGCGGCGCCC
GTCTGGGACCGCATCGGCAGCACCGTCTTCGAACACGGCGAGCGCTTCTACAACTTCAAAGGCCTGCGGGCATTCAAATC
AAAATTTCATCCGCACTGGCAACCGCGCTATCTTGCGGTCTCCGGAGGGGGCAATCCGATGATCGCGTTGATGGACGCGA
CATTTCTGATCGGGGGCGGATTGAAAGGGGTAGTGAGAAAATGA

Upstream 100 bases:

>100_bases
TGGGGTTGCGGCGGCATGTCTTCATAGAGCTTCTATGCCTCTCATGTTACGTCTTTCAAAAATGATTCCCGCCGAGCTGG
CAATTGCAGCAGGATAAGAG

Downstream 100 bases:

>100_bases
TCAGGACAATCCTTCTTGCCACGGCATGCGCCTGCATGCTCGCGGCCGCACCTGCACGCGCCGCTGAGGAAACCACGCAG
AGCTTTGAAACCGGGCTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: Lysylphosphatidylglycerol synthase; LPG synthase; Multiple peptide resistance factor [H]

Number of amino acids: Translated: 867; Mature: 866

Protein sequence:

>867_residues
MSGHDNLKEIEDAEGFSFRGLFRRYRTPLTAAATLVVFCLVGYAIMQLTNEVRYDDVVDALAATRPSAILLALFFTALSF
LSLVFYDLNAIEYIGKKLPFPHVALTAFSAYAVGNTAGFGALSGGAIRYRAYTRLGLSPEDIGRIIAFVTLSFGLGLAGV
GAIALIIIADEIGPLIGVSPFLLRLIAGSIVAILGAVMIIGRDGRVLDLGPVAIRLPDSRTWSRQFLVTAFDIAASASVL
YVLLPQTAIGWPVFLAVYAIAVGLGVLSHVPAGLGVFETVIIASLGSAVNIDAVLGSLVLYRLIYHVLPLLIAVLAVSAT
ELRRFVDHPAASSVRRIGGRLMPQLLSALALLLGVMLIFSSVTPTPDQNLEFLSNYLPLPMVEGAHFLSSLLGLALVVAA
RGLGQRLDGAWWVAVFSALAALTLSLLKAIALVEAAFLAFLIFGLFVSRRLFTRHASLLNQALTASWLMAIAVIVVGAVV
ILLFVYRDVEYSNQLWWQFEFTAEAPRGLRAVLGITIISSAIAIFSLLRPATFRPEPATEEALARAVEIVGKQGNADANL
VRMGDKSIMFSEKGDAFIMYGRQGRSWIALFDPVGEHRAGQELVWRFVEAARAAGCRAVFYQISPSLLSHCADAGLRAFK
LGELAVTDLRTFEMKGGKWANLRQTASRAQRDGLEFAVVEPEDVPSVIDDLSAVSTAWLEHHNAKEKGFSLGAFDPDYVS
SQPVGILKKDGRIVAFANILVTESRQEGTIDLMRFSPDAPKGSMDFLFVQIMEYLRGQGFTHFNLGMAPLSGMSKREAAP
VWDRIGSTVFEHGERFYNFKGLRAFKSKFHPHWQPRYLAVSGGGNPMIALMDATFLIGGGLKGVVRK

Sequences:

>Translated_867_residues
MSGHDNLKEIEDAEGFSFRGLFRRYRTPLTAAATLVVFCLVGYAIMQLTNEVRYDDVVDALAATRPSAILLALFFTALSF
LSLVFYDLNAIEYIGKKLPFPHVALTAFSAYAVGNTAGFGALSGGAIRYRAYTRLGLSPEDIGRIIAFVTLSFGLGLAGV
GAIALIIIADEIGPLIGVSPFLLRLIAGSIVAILGAVMIIGRDGRVLDLGPVAIRLPDSRTWSRQFLVTAFDIAASASVL
YVLLPQTAIGWPVFLAVYAIAVGLGVLSHVPAGLGVFETVIIASLGSAVNIDAVLGSLVLYRLIYHVLPLLIAVLAVSAT
ELRRFVDHPAASSVRRIGGRLMPQLLSALALLLGVMLIFSSVTPTPDQNLEFLSNYLPLPMVEGAHFLSSLLGLALVVAA
RGLGQRLDGAWWVAVFSALAALTLSLLKAIALVEAAFLAFLIFGLFVSRRLFTRHASLLNQALTASWLMAIAVIVVGAVV
ILLFVYRDVEYSNQLWWQFEFTAEAPRGLRAVLGITIISSAIAIFSLLRPATFRPEPATEEALARAVEIVGKQGNADANL
VRMGDKSIMFSEKGDAFIMYGRQGRSWIALFDPVGEHRAGQELVWRFVEAARAAGCRAVFYQISPSLLSHCADAGLRAFK
LGELAVTDLRTFEMKGGKWANLRQTASRAQRDGLEFAVVEPEDVPSVIDDLSAVSTAWLEHHNAKEKGFSLGAFDPDYVS
SQPVGILKKDGRIVAFANILVTESRQEGTIDLMRFSPDAPKGSMDFLFVQIMEYLRGQGFTHFNLGMAPLSGMSKREAAP
VWDRIGSTVFEHGERFYNFKGLRAFKSKFHPHWQPRYLAVSGGGNPMIALMDATFLIGGGLKGVVRK
>Mature_866_residues
SGHDNLKEIEDAEGFSFRGLFRRYRTPLTAAATLVVFCLVGYAIMQLTNEVRYDDVVDALAATRPSAILLALFFTALSFL
SLVFYDLNAIEYIGKKLPFPHVALTAFSAYAVGNTAGFGALSGGAIRYRAYTRLGLSPEDIGRIIAFVTLSFGLGLAGVG
AIALIIIADEIGPLIGVSPFLLRLIAGSIVAILGAVMIIGRDGRVLDLGPVAIRLPDSRTWSRQFLVTAFDIAASASVLY
VLLPQTAIGWPVFLAVYAIAVGLGVLSHVPAGLGVFETVIIASLGSAVNIDAVLGSLVLYRLIYHVLPLLIAVLAVSATE
LRRFVDHPAASSVRRIGGRLMPQLLSALALLLGVMLIFSSVTPTPDQNLEFLSNYLPLPMVEGAHFLSSLLGLALVVAAR
GLGQRLDGAWWVAVFSALAALTLSLLKAIALVEAAFLAFLIFGLFVSRRLFTRHASLLNQALTASWLMAIAVIVVGAVVI
LLFVYRDVEYSNQLWWQFEFTAEAPRGLRAVLGITIISSAIAIFSLLRPATFRPEPATEEALARAVEIVGKQGNADANLV
RMGDKSIMFSEKGDAFIMYGRQGRSWIALFDPVGEHRAGQELVWRFVEAARAAGCRAVFYQISPSLLSHCADAGLRAFKL
GELAVTDLRTFEMKGGKWANLRQTASRAQRDGLEFAVVEPEDVPSVIDDLSAVSTAWLEHHNAKEKGFSLGAFDPDYVSS
QPVGILKKDGRIVAFANILVTESRQEGTIDLMRFSPDAPKGSMDFLFVQIMEYLRGQGFTHFNLGMAPLSGMSKREAAPV
WDRIGSTVFEHGERFYNFKGLRAFKSKFHPHWQPRYLAVSGGGNPMIALMDATFLIGGGLKGVVRK

Specific function: Catalyzes the transfer of a lysyl group from L-lysyl- tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), a major component of the bacterial membrane with a positive net charge. LPG synthesis contributes

COG id: COG2898

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LPG synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787006, Length=277, Percent_Identity=27.0758122743682, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR022791 [H]

Pfam domain/function: PF03706 UPF0104 [H]

EC number: =2.3.2.3 [H]

Molecular weight: Translated: 93874; Mature: 93743

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHDNLKEIEDAEGFSFRGLFRRYRTPLTAAATLVVFCLVGYAIMQLTNEVRYDDVVDA
CCCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAATRPSAILLALFFTALSFLSLVFYDLNAIEYIGKKLPFPHVALTAFSAYAVGNTAGFG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
ALSGGAIRYRAYTRLGLSPEDIGRIIAFVTLSFGLGLAGVGAIALIIIADEIGPLIGVSP
CCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
FLLRLIAGSIVAILGAVMIIGRDGRVLDLGPVAIRLPDSRTWSRQFLVTAFDIAASASVL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCEE
YVLLPQTAIGWPVFLAVYAIAVGLGVLSHVPAGLGVFETVIIASLGSAVNIDAVLGSLVL
EEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
YRLIYHVLPLLIAVLAVSATELRRFVDHPAASSVRRIGGRLMPQLLSALALLLGVMLIFS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTPTPDQNLEFLSNYLPLPMVEGAHFLSSLLGLALVVAARGLGQRLDGAWWVAVFSALA
CCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
ALTLSLLKAIALVEAAFLAFLIFGLFVSRRLFTRHASLLNQALTASWLMAIAVIVVGAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLFVYRDVEYSNQLWWQFEFTAEAPRGLRAVLGITIISSAIAIFSLLRPATFRPEPATE
HHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EALARAVEIVGKQGNADANLVRMGDKSIMFSEKGDAFIMYGRQGRSWIALFDPVGEHRAG
HHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCC
QELVWRFVEAARAAGCRAVFYQISPSLLSHCADAGLRAFKLGELAVTDLRTFEMKGGKWA
HHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHEECCHHHHHHHHEECCCCCCC
NLRQTASRAQRDGLEFAVVEPEDVPSVIDDLSAVSTAWLEHHNAKEKGFSLGAFDPDYVS
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
SQPVGILKKDGRIVAFANILVTESRQEGTIDLMRFSPDAPKGSMDFLFVQIMEYLRGQGF
CCCCCEEECCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
THFNLGMAPLSGMSKREAAPVWDRIGSTVFEHGERFYNFKGLRAFKSKFHPHWQPRYLAV
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCCCEEEE
SGGGNPMIALMDATFLIGGGLKGVVRK
ECCCCCEEEEEHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SGHDNLKEIEDAEGFSFRGLFRRYRTPLTAAATLVVFCLVGYAIMQLTNEVRYDDVVDA
CCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAATRPSAILLALFFTALSFLSLVFYDLNAIEYIGKKLPFPHVALTAFSAYAVGNTAGFG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
ALSGGAIRYRAYTRLGLSPEDIGRIIAFVTLSFGLGLAGVGAIALIIIADEIGPLIGVSP
CCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHH
FLLRLIAGSIVAILGAVMIIGRDGRVLDLGPVAIRLPDSRTWSRQFLVTAFDIAASASVL
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCEE
YVLLPQTAIGWPVFLAVYAIAVGLGVLSHVPAGLGVFETVIIASLGSAVNIDAVLGSLVL
EEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
YRLIYHVLPLLIAVLAVSATELRRFVDHPAASSVRRIGGRLMPQLLSALALLLGVMLIFS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTPTPDQNLEFLSNYLPLPMVEGAHFLSSLLGLALVVAARGLGQRLDGAWWVAVFSALA
CCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
ALTLSLLKAIALVEAAFLAFLIFGLFVSRRLFTRHASLLNQALTASWLMAIAVIVVGAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLFVYRDVEYSNQLWWQFEFTAEAPRGLRAVLGITIISSAIAIFSLLRPATFRPEPATE
HHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
EALARAVEIVGKQGNADANLVRMGDKSIMFSEKGDAFIMYGRQGRSWIALFDPVGEHRAG
HHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCEEEEECCCCCCEEEEECCCCCCCCC
QELVWRFVEAARAAGCRAVFYQISPSLLSHCADAGLRAFKLGELAVTDLRTFEMKGGKWA
HHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHCCCHHHEECCHHHHHHHHEECCCCCCC
NLRQTASRAQRDGLEFAVVEPEDVPSVIDDLSAVSTAWLEHHNAKEKGFSLGAFDPDYVS
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
SQPVGILKKDGRIVAFANILVTESRQEGTIDLMRFSPDAPKGSMDFLFVQIMEYLRGQGF
CCCCCEEECCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
THFNLGMAPLSGMSKREAAPVWDRIGSTVFEHGERFYNFKGLRAFKSKFHPHWQPRYLAV
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHHCCCCCCCCEEEE
SGGGNPMIALMDATFLIGGGLKGVVRK
ECCCCCEEEEEHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11342591; 12496209 [H]